N-acetylcysteine supplementation remodels thiol-related biochemical pathways towards decreased oxidation in diabetic submandibular glands
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/13169 |
Resumo: | Secondary disorders in consequences to diabetes involves the development of several diseases in the oral cavity, as periodontitis, xerostomy, infection by diverse pathogens and dysfunctions on the salivary secretion. These alterations occur partially, in consequence of the oxidative stress occasioned by hyperglycemia, and are important in patients undiagnosed or that have flaws in their therapeutic process. The aim of this work was to evaluate biochemical alterations of submandibular glands in response to oxidative stress during diabetes mellitus, and verify the effects of N-acetylcystein supplementation to diabetic rats, specially on the regulation of modifications related to glutathione and thiol proteins. For this purpose, the levels of some oxidative stress markers and the occurrence of the post-translational event of S-glutathionylation were evaluated. The a-amilase degranulation by isolated acinar cells and glandular relative weight was also measured for each experimental group. The compound was able to decrease the lipoperoxidation and proteic oxidation observed in the submandibular gland of diabetic rats, preventing the decrease of the tecidual reducing power and increasing the occurrence of the post-translational process of S-glutathionylation. The diabetic condition increases the degranulation of a-amilase and the glandular weight, but the supplementation with N-acetylcystein did not affect these events. Together these findings may help to elucidate the status of oxidative stress on salivary glands and suggest new therapeutic strategies employing antioxidants of low molecular weight to prevent oral and systemic dysfunctions related to diabetes. |
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N-acetylcysteine supplementation remodels thiol-related biochemical pathways towards decreased oxidation in diabetic submandibular glandsLa suplementación con N-acetilcisteína remodela las vías bioquímicas relacionadas con el tiol hacia una oxidación disminuida en las glândulas submandibulares diabéticasA suplementação de N-acetilcisteína remodela as vias bioquímicas relacionadas ao tiol em direção à redução da oxidação nas glândulas submandibulares diabéticasDiabetes MellitusSubmandibular glandOxidative stressN-acetylcystein.Diabetes MellitusGlándula submandibularEstrés oxidativoN-acetilcisteína.Diabetes MellitusGlândula submandibularEstresse oxidativoN-acetilcisteína.Secondary disorders in consequences to diabetes involves the development of several diseases in the oral cavity, as periodontitis, xerostomy, infection by diverse pathogens and dysfunctions on the salivary secretion. These alterations occur partially, in consequence of the oxidative stress occasioned by hyperglycemia, and are important in patients undiagnosed or that have flaws in their therapeutic process. The aim of this work was to evaluate biochemical alterations of submandibular glands in response to oxidative stress during diabetes mellitus, and verify the effects of N-acetylcystein supplementation to diabetic rats, specially on the regulation of modifications related to glutathione and thiol proteins. For this purpose, the levels of some oxidative stress markers and the occurrence of the post-translational event of S-glutathionylation were evaluated. The a-amilase degranulation by isolated acinar cells and glandular relative weight was also measured for each experimental group. The compound was able to decrease the lipoperoxidation and proteic oxidation observed in the submandibular gland of diabetic rats, preventing the decrease of the tecidual reducing power and increasing the occurrence of the post-translational process of S-glutathionylation. The diabetic condition increases the degranulation of a-amilase and the glandular weight, but the supplementation with N-acetylcystein did not affect these events. Together these findings may help to elucidate the status of oxidative stress on salivary glands and suggest new therapeutic strategies employing antioxidants of low molecular weight to prevent oral and systemic dysfunctions related to diabetes.Los trastornos secundarios que resultan de la diabetes implican el desarrollo de diversas enfermedades en la cavidad bucal, como periodontitis, xerostomía, infección por diversos patógenos y disfunciones en la secreción salival. Estos cambios ocurren parcialmente, debido al estrés oxidativo causado por la hiperglucemia, y son importantes en pacientes que no están diagnosticados o tienen fallas en el proceso terapéutico. El objetivo de este trabajo fue evaluar las alteraciones bioquímicas de las glándulas submandibulares en respuesta al estrés oxidativo durante la diabetes mellitus, y verificar la combinación farmacológica de la suplementación con N-acetilcisteína en ratas diabéticas, especialmente en la regulación de cambios relacionados con el glutatión. y proteínas de tiol. Para ello, se evaluaron los niveles de algunos marcadores de estrés oxidativo y la ocurrencia del evento de S-glutatilación postraduccional. También se midieron la desgranulación de la a-amilasa por las células acinares aisladas y el peso relativo de la glándula para cada grupo experimental. El compuesto fue capaz de disminuir la lipoperoxidación y oxidación proteica observada en la glándula submandibular de ratas diabéticas, evitando la reducción del poder reductor tisular y aumentando la ocurrencia del proceso postraduccional de S-glutatilación. La condición diabética aumenta la desgranulación de a-amilasa y el peso glandular, pero la suplementación con N-acetilcisteína no afectó estos eventos. Juntos, estos hallazgos pueden ayudar a dilucidar el estado de estrés oxidativo en las glándulas salivales y sugerir nuevas estrategias terapéuticas que utilizan antioxidantes de bajo peso molecular para prevenir los trastornos orales y sistémicos relacionados con la diabetes.Desordens secundárias em consequências da diabetes envolvem o desenvolvimento de diversas doenças na cavidade oral, como periodontite, xerostomia, infecção por diversos patógenos e disfunções na secreção salivar. Essas alterações ocorrem parcialmente, em decorrência do estresse oxidativo ocasionado pela hiperglicemia, e são importantes em pacientes não diagnosticados ou que apresentam falhas no processo terapêutico. O objetivo deste trabalho foi avaliar as alterações bioquímicas das glândulas submandibulares em resposta ao estresse oxidativo durante o diabetes mellitus, e verificar o conjunto farmacológico da suplementação de N-acetilcisteína em ratos diabéticos, especialmente na regulação das modificações relacionadas às proteínas glutationa e tiol. Para tanto, foram avaliados os níveis de alguns marcadores de estresse oxidativo e a ocorrência do evento pós-translacional de S-glutationilação. A degranulação da a-amilase por células acinares isoladas e o peso relativo glandular também foram medidos para cada grupo experimental. O composto foi capaz de diminuir a lipoperoxidação e oxidação protéica observadas na glândula submandibular de ratos diabéticos, evitando a diminuição do poder redutor tecidual e aumentando a ocorrência do processo pós-translacional de S-glutationilação. A condição diabética aumenta a degranulação da a-amilase e o peso glandular, mas a suplementação com N-acetilcisteína não afetou esses eventos. Juntos, esses achados podem ajudar a elucidar o estado do estresse oxidativo nas glândulas salivares e sugerir novas estratégias terapêuticas empregando antioxidantes de baixo peso molecular para prevenir disfunções orais e sistêmicas relacionadas ao diabetes.Research, Society and Development2021-03-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1316910.33448/rsd-v10i3.13169Research, Society and Development; Vol. 10 No. 3; e14510313169Research, Society and Development; Vol. 10 Núm. 3; e14510313169Research, Society and Development; v. 10 n. 3; e145103131692525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/13169/11835Copyright (c) 2021 Aluísio Eustáquio de Freitas Miranda Filho; Ana Carolina Guimarães Ribeiro; Nadine Francine Marcula Linhares Nunes; Samuel Nuno Pereira Lima; Vinicio Felipe Brasil Rocha; Francisco Rafael Martins Laurindo; Gérsika Bitencourt Santos; Maísa Ribeiro Pereira Lima Brigagão https://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessMiranda Filho, Aluísio Eustáquio de FreitasRibeiro, Ana Carolina Guimarães Nunes, Nadine Francine Marcula Linhares Lima, Samuel Nuno Pereira Rocha, Vinicio Felipe Brasil Laurindo, Francisco Rafael Martins Santos, Gérsika Bitencourt Brigagão , Maísa Ribeiro Pereira Lima 2021-03-28T12:03:35Zoai:ojs.pkp.sfu.ca:article/13169Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:34:34.583527Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
N-acetylcysteine supplementation remodels thiol-related biochemical pathways towards decreased oxidation in diabetic submandibular glands La suplementación con N-acetilcisteína remodela las vías bioquímicas relacionadas con el tiol hacia una oxidación disminuida en las glândulas submandibulares diabéticas A suplementação de N-acetilcisteína remodela as vias bioquímicas relacionadas ao tiol em direção à redução da oxidação nas glândulas submandibulares diabéticas |
title |
N-acetylcysteine supplementation remodels thiol-related biochemical pathways towards decreased oxidation in diabetic submandibular glands |
spellingShingle |
N-acetylcysteine supplementation remodels thiol-related biochemical pathways towards decreased oxidation in diabetic submandibular glands Miranda Filho, Aluísio Eustáquio de Freitas Diabetes Mellitus Submandibular gland Oxidative stress N-acetylcystein. Diabetes Mellitus Glándula submandibular Estrés oxidativo N-acetilcisteína. Diabetes Mellitus Glândula submandibular Estresse oxidativo N-acetilcisteína. |
title_short |
N-acetylcysteine supplementation remodels thiol-related biochemical pathways towards decreased oxidation in diabetic submandibular glands |
title_full |
N-acetylcysteine supplementation remodels thiol-related biochemical pathways towards decreased oxidation in diabetic submandibular glands |
title_fullStr |
N-acetylcysteine supplementation remodels thiol-related biochemical pathways towards decreased oxidation in diabetic submandibular glands |
title_full_unstemmed |
N-acetylcysteine supplementation remodels thiol-related biochemical pathways towards decreased oxidation in diabetic submandibular glands |
title_sort |
N-acetylcysteine supplementation remodels thiol-related biochemical pathways towards decreased oxidation in diabetic submandibular glands |
author |
Miranda Filho, Aluísio Eustáquio de Freitas |
author_facet |
Miranda Filho, Aluísio Eustáquio de Freitas Ribeiro, Ana Carolina Guimarães Nunes, Nadine Francine Marcula Linhares Lima, Samuel Nuno Pereira Rocha, Vinicio Felipe Brasil Laurindo, Francisco Rafael Martins Santos, Gérsika Bitencourt Brigagão , Maísa Ribeiro Pereira Lima |
author_role |
author |
author2 |
Ribeiro, Ana Carolina Guimarães Nunes, Nadine Francine Marcula Linhares Lima, Samuel Nuno Pereira Rocha, Vinicio Felipe Brasil Laurindo, Francisco Rafael Martins Santos, Gérsika Bitencourt Brigagão , Maísa Ribeiro Pereira Lima |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Miranda Filho, Aluísio Eustáquio de Freitas Ribeiro, Ana Carolina Guimarães Nunes, Nadine Francine Marcula Linhares Lima, Samuel Nuno Pereira Rocha, Vinicio Felipe Brasil Laurindo, Francisco Rafael Martins Santos, Gérsika Bitencourt Brigagão , Maísa Ribeiro Pereira Lima |
dc.subject.por.fl_str_mv |
Diabetes Mellitus Submandibular gland Oxidative stress N-acetylcystein. Diabetes Mellitus Glándula submandibular Estrés oxidativo N-acetilcisteína. Diabetes Mellitus Glândula submandibular Estresse oxidativo N-acetilcisteína. |
topic |
Diabetes Mellitus Submandibular gland Oxidative stress N-acetylcystein. Diabetes Mellitus Glándula submandibular Estrés oxidativo N-acetilcisteína. Diabetes Mellitus Glândula submandibular Estresse oxidativo N-acetilcisteína. |
description |
Secondary disorders in consequences to diabetes involves the development of several diseases in the oral cavity, as periodontitis, xerostomy, infection by diverse pathogens and dysfunctions on the salivary secretion. These alterations occur partially, in consequence of the oxidative stress occasioned by hyperglycemia, and are important in patients undiagnosed or that have flaws in their therapeutic process. The aim of this work was to evaluate biochemical alterations of submandibular glands in response to oxidative stress during diabetes mellitus, and verify the effects of N-acetylcystein supplementation to diabetic rats, specially on the regulation of modifications related to glutathione and thiol proteins. For this purpose, the levels of some oxidative stress markers and the occurrence of the post-translational event of S-glutathionylation were evaluated. The a-amilase degranulation by isolated acinar cells and glandular relative weight was also measured for each experimental group. The compound was able to decrease the lipoperoxidation and proteic oxidation observed in the submandibular gland of diabetic rats, preventing the decrease of the tecidual reducing power and increasing the occurrence of the post-translational process of S-glutathionylation. The diabetic condition increases the degranulation of a-amilase and the glandular weight, but the supplementation with N-acetylcystein did not affect these events. Together these findings may help to elucidate the status of oxidative stress on salivary glands and suggest new therapeutic strategies employing antioxidants of low molecular weight to prevent oral and systemic dysfunctions related to diabetes. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-03-10 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/13169 10.33448/rsd-v10i3.13169 |
url |
https://rsdjournal.org/index.php/rsd/article/view/13169 |
identifier_str_mv |
10.33448/rsd-v10i3.13169 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/13169/11835 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 3; e14510313169 Research, Society and Development; Vol. 10 Núm. 3; e14510313169 Research, Society and Development; v. 10 n. 3; e14510313169 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052784040738816 |