The role of local irradiation timing on the osseointegration process in implants

Detalhes bibliográficos
Autor(a) principal: Vasconcellos, Luana Marotta Reis de
Data de Publicação: 2022
Outros Autores: Kamezawa, Leonardo Silva Gomes, Costa, Bruno César Almeida, Theodoro, Ana Luisa, Silva, Camila Duarte da, Tango, Estela Kaminagakura, Silva, Leonardo Alvares Sobral, Federico, Claudio Antonio, Tango, Rubens Nisie
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/29923
Resumo: Purpose: The objective of this study was to evaluate the effects of Gamma irradiation by simulating a total dose of conventional head and neck cancer radiotherapy on bone remodeling and osseointegration of implants in femurs of rats.Materials and Methods: Sixty rats received threaded titanium (Ti6Al4V) implants in both femurs. The animals were divided: No-Irradiation (N-Ir): N-Ir group with implant solely, Early-Irradiation (E-Ir): implant + irradiation after 24hrs.; Late-Irradiation (L-Ir): implant + irradiation after 4 weeks and Previous-Irradiation (P-Ir): irradiation + implant after 4 weeks. Irradiation was fractioned in two sessions of 15 Gy each, totaling 30 Gy. The animals were euthanized at 3 days, 2 and 7 weeks after treatment, which were chosen from previous studies. Runx2 immunohistochemical markers and Picrosirius red stain, histomorphometric analysis (BIC and BAFO) and reverse torque test were used to evaluate the implant/bone interface and surrounding bone. Data of Runx-2 (%), picrosirius red stain (%), BIC and BAFO (%), and reverse torque (N/cm) was submitted to ANOVA and Tukey tests for comparison among groups, both with α=0.05. Results: The results showed that Runx-2 expression was different in irradiated groups when compared to N-Ir group. In general, the collagen fiber analyses in the irradiated groups differed from the N-Ir group. Regarding BIC, BAFO, and the reverse torque test, the L-Ir group demonstrated results similar to the N-Ir group, while the others groups were impaired by radiotherapy. Conclusions: Ionizing radiation negatively affected bone remodeling, and its effect depends on when radiotherapy is applied, with a delay of osseointegration when applied previously or close to surgery.
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spelling The role of local irradiation timing on the osseointegration process in implantsEl papel del tiempo de irradiación local en el proceso de osteointegración en implantesO papel do tempo de irradiação local no processo de osseointegração em implantesOsseointegrationRadiotherapyImmunohistochemistryTorque.OsteointegraciónRadioterapiaInmunohistoquímicaTorsíon.OsseointegraçãoRadioterapiaImuno-histoquímicaTorque.Purpose: The objective of this study was to evaluate the effects of Gamma irradiation by simulating a total dose of conventional head and neck cancer radiotherapy on bone remodeling and osseointegration of implants in femurs of rats.Materials and Methods: Sixty rats received threaded titanium (Ti6Al4V) implants in both femurs. The animals were divided: No-Irradiation (N-Ir): N-Ir group with implant solely, Early-Irradiation (E-Ir): implant + irradiation after 24hrs.; Late-Irradiation (L-Ir): implant + irradiation after 4 weeks and Previous-Irradiation (P-Ir): irradiation + implant after 4 weeks. Irradiation was fractioned in two sessions of 15 Gy each, totaling 30 Gy. The animals were euthanized at 3 days, 2 and 7 weeks after treatment, which were chosen from previous studies. Runx2 immunohistochemical markers and Picrosirius red stain, histomorphometric analysis (BIC and BAFO) and reverse torque test were used to evaluate the implant/bone interface and surrounding bone. Data of Runx-2 (%), picrosirius red stain (%), BIC and BAFO (%), and reverse torque (N/cm) was submitted to ANOVA and Tukey tests for comparison among groups, both with α=0.05. Results: The results showed that Runx-2 expression was different in irradiated groups when compared to N-Ir group. In general, the collagen fiber analyses in the irradiated groups differed from the N-Ir group. Regarding BIC, BAFO, and the reverse torque test, the L-Ir group demonstrated results similar to the N-Ir group, while the others groups were impaired by radiotherapy. Conclusions: Ionizing radiation negatively affected bone remodeling, and its effect depends on when radiotherapy is applied, with a delay of osseointegration when applied previously or close to surgery.Propósito: El objetivo de este estudio fue evaluar los efectos de la radiación Gamma mediante la simulación de una dosis total de radioterapia convencional para el cáncer de cabeza y cuello en la remodelación ósea y la osteointegración de implantes en fémures de ratas.Materiales y Métodos: Sesenta ratas recibieron implantes roscados de titanio (Ti6Al4V) en ambos fémures. Los animales se dividieron: Sin Irradiación (N-Ir): grupo N-Ir con implante solo, Irradiación Temprana (E-Ir): implante + irradiación después de 24 h; Irradiación tardía (L-Ir): implante + irradiación después de 4 semanas e Irradiación previa (P-Ir): irradiación + implante después de 4 semanas. La irradiación se fraccionó en dos sesiones de 15 Gy cada una, totalizando 30 Gy. Los animales fueron sacrificados a los 3 días, 2 y 7 semanas después del tratamiento. Se utilizaron marcadores inmunohistoquímicos Runx2 y tinción roja de Picrosirius, análisis histomorfométrico (BIC y BAFO) y prueba de torsión inversa para evaluar la interfaz implante/hueso y el hueso circundante. Los datos de Runx-2 (%), tinción roja de picrosirius (%), BIC y BAFO (%) y torque inverso (N/cm) se sometieron a las pruebas de ANOVA y Tukey para la comparación entre grupos, ambas con α=0,05. Resultados: Los resultados mostraron que la expresión de Runx-2 fue diferente en los grupos irradiados en comparación con el grupo N-Ir. En general, los análisis de fibra de colágeno en los grupos irradiados diferían del grupo N-Ir. Con respecto a BIC, BAFO y la prueba de torsión inversa, el grupo L-Ir mostró resultados similares al grupo N-Ir, mientras que los otros grupos se vieron afectados por la radioterapia.Conclusiones: Las radiaciones ionizantes afectan negativamente al remodelado óseo, y su efecto depende del momento de aplicación de la radioterapia, con un retraso de la osteointegración cuando se aplica de forma previa o próxima a la cirugía.Objetivo: O objetivo deste estudo foi avaliar os efeitos da irradiação gama simulando uma dose total de radioterapia convencional em câncer de cabeça e pescoço na remodelação óssea e osseointegração de implantes em fêmures de ratos.Materiais e Métodos: Sessenta ratos receberam implantes de titânio rosqueado (Ti6Al4V) em ambos os fêmures. Os animais foram divididos: Sem Irradiação (N-Ir): Grupo N-Ir com implante somente; Irradiação Precoce (E-Ir): implante + irradiação após 24h; Irradiação Tardia (L-Ir): implante + irradiação após 4 semanas e Irradiação Prévia (P-Ir): irradiação + implante após 4 semanas. A irradiação foi fracionada em duas sessões de 15 Gy cada, totalizando 30 Gy. Os animais foram sacrificados aos 3 dias, 2 e 7 semanas após o tratamento. Marcadores imuno-histoquímicos Runx2 e coloração Picrosirius red, análise histomorfométrica (BIC e BAFO) e teste de torque reverso foram utilizados para avaliar a interface implante/osso e osso circundante. Os dados de Runx-2 (%), coloração picrosirius red (%), BIC e BAFO (%) e torque reverso (N/cm) foram submetidos aos testes ANOVA e Tukey para comparação entre grupos, ambos com α=0,05.Resultados: Os resultados mostraram que a expressão de Runx-2 foi diferente nos grupos irradiados quando comparados ao grupo N-Ir. Em geral, as análises de fibras colágenas nos grupos irradiados diferiram do grupo N-Ir. Em relação ao BIC, BAFO e teste de torque reverso, o grupo L-Ir apresentou resultados semelhantes ao grupo N-Ir, enquanto os demais grupos foram prejudicados pela radioterapia.Conclusões: A radiação ionizante afetou negativamente a remodelação óssea, e seu efeito depende de quando a radioterapia é aplicada, com retardo da osseointegração quando aplicada previamente ou próximo à cirurgia.Research, Society and Development2022-06-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2992310.33448/rsd-v11i8.29923Research, Society and Development; Vol. 11 No. 8; e5711829923Research, Society and Development; Vol. 11 Núm. 8; e5711829923Research, Society and Development; v. 11 n. 8; e57118299232525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/29923/26266Copyright (c) 2022 Luana Marotta Reis de Vasconcellos; Leonardo Silva Gomes Kamezawa; Bruno César Almeida Costa; Ana Luisa Theodoro; Camila Duarte da Silva; Estela Kaminagakura Tango; Leonardo Alvares Sobral Silva; Claudio Antonio Federico; Rubens Nisie Tangohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessVasconcellos, Luana Marotta Reis de Kamezawa, Leonardo Silva Gomes Costa, Bruno César Almeida Theodoro, Ana Luisa Silva, Camila Duarte da Tango, Estela Kaminagakura Silva, Leonardo Alvares Sobral Federico, Claudio Antonio Tango, Rubens Nisie 2022-07-01T13:34:06Zoai:ojs.pkp.sfu.ca:article/29923Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:46:51.748018Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv The role of local irradiation timing on the osseointegration process in implants
El papel del tiempo de irradiación local en el proceso de osteointegración en implantes
O papel do tempo de irradiação local no processo de osseointegração em implantes
title The role of local irradiation timing on the osseointegration process in implants
spellingShingle The role of local irradiation timing on the osseointegration process in implants
Vasconcellos, Luana Marotta Reis de
Osseointegration
Radiotherapy
Immunohistochemistry
Torque.
Osteointegración
Radioterapia
Inmunohistoquímica
Torsíon.
Osseointegração
Radioterapia
Imuno-histoquímica
Torque.
title_short The role of local irradiation timing on the osseointegration process in implants
title_full The role of local irradiation timing on the osseointegration process in implants
title_fullStr The role of local irradiation timing on the osseointegration process in implants
title_full_unstemmed The role of local irradiation timing on the osseointegration process in implants
title_sort The role of local irradiation timing on the osseointegration process in implants
author Vasconcellos, Luana Marotta Reis de
author_facet Vasconcellos, Luana Marotta Reis de
Kamezawa, Leonardo Silva Gomes
Costa, Bruno César Almeida
Theodoro, Ana Luisa
Silva, Camila Duarte da
Tango, Estela Kaminagakura
Silva, Leonardo Alvares Sobral
Federico, Claudio Antonio
Tango, Rubens Nisie
author_role author
author2 Kamezawa, Leonardo Silva Gomes
Costa, Bruno César Almeida
Theodoro, Ana Luisa
Silva, Camila Duarte da
Tango, Estela Kaminagakura
Silva, Leonardo Alvares Sobral
Federico, Claudio Antonio
Tango, Rubens Nisie
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Vasconcellos, Luana Marotta Reis de
Kamezawa, Leonardo Silva Gomes
Costa, Bruno César Almeida
Theodoro, Ana Luisa
Silva, Camila Duarte da
Tango, Estela Kaminagakura
Silva, Leonardo Alvares Sobral
Federico, Claudio Antonio
Tango, Rubens Nisie
dc.subject.por.fl_str_mv Osseointegration
Radiotherapy
Immunohistochemistry
Torque.
Osteointegración
Radioterapia
Inmunohistoquímica
Torsíon.
Osseointegração
Radioterapia
Imuno-histoquímica
Torque.
topic Osseointegration
Radiotherapy
Immunohistochemistry
Torque.
Osteointegración
Radioterapia
Inmunohistoquímica
Torsíon.
Osseointegração
Radioterapia
Imuno-histoquímica
Torque.
description Purpose: The objective of this study was to evaluate the effects of Gamma irradiation by simulating a total dose of conventional head and neck cancer radiotherapy on bone remodeling and osseointegration of implants in femurs of rats.Materials and Methods: Sixty rats received threaded titanium (Ti6Al4V) implants in both femurs. The animals were divided: No-Irradiation (N-Ir): N-Ir group with implant solely, Early-Irradiation (E-Ir): implant + irradiation after 24hrs.; Late-Irradiation (L-Ir): implant + irradiation after 4 weeks and Previous-Irradiation (P-Ir): irradiation + implant after 4 weeks. Irradiation was fractioned in two sessions of 15 Gy each, totaling 30 Gy. The animals were euthanized at 3 days, 2 and 7 weeks after treatment, which were chosen from previous studies. Runx2 immunohistochemical markers and Picrosirius red stain, histomorphometric analysis (BIC and BAFO) and reverse torque test were used to evaluate the implant/bone interface and surrounding bone. Data of Runx-2 (%), picrosirius red stain (%), BIC and BAFO (%), and reverse torque (N/cm) was submitted to ANOVA and Tukey tests for comparison among groups, both with α=0.05. Results: The results showed that Runx-2 expression was different in irradiated groups when compared to N-Ir group. In general, the collagen fiber analyses in the irradiated groups differed from the N-Ir group. Regarding BIC, BAFO, and the reverse torque test, the L-Ir group demonstrated results similar to the N-Ir group, while the others groups were impaired by radiotherapy. Conclusions: Ionizing radiation negatively affected bone remodeling, and its effect depends on when radiotherapy is applied, with a delay of osseointegration when applied previously or close to surgery.
publishDate 2022
dc.date.none.fl_str_mv 2022-06-08
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/29923
10.33448/rsd-v11i8.29923
url https://rsdjournal.org/index.php/rsd/article/view/29923
identifier_str_mv 10.33448/rsd-v11i8.29923
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/29923/26266
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 11 No. 8; e5711829923
Research, Society and Development; Vol. 11 Núm. 8; e5711829923
Research, Society and Development; v. 11 n. 8; e5711829923
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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