Stem cell markers expression evaluation in Oral Squamous Cell Carcinoma
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/20840 |
Resumo: | Oral carcinogenesis is a highly complex process. Molecular studies demonstrate that some oral cancers progress from pre-neoplastic lesions. Cancer stem cells (CSCs), a small cellular group within the tumor, have currently received much attention by its self-renewal and tumor differentiation ability. It is considered that CSCs may be responsible for the relapse and metastasis of cancer. CSCs have been reported to express common embryonic stem cells markers such as Nestin and Nanog. The aim of the present study was to identify the presence of Nestin and Nanog markers in patients with oral squamous cell carcinoma (OSCC). Nine samples were collected from patients diagnosed with OSCC and two healthy oral mucosa were used as control. The markers expression in nine OSCC was determined by end-point PCR. The OSCC population analyzed was mostly composed of black and white males, with a mean age of 70.6 years, that reported alcohol and tobacco consumption. Nestin and Nanog stem cell markers were expressed in all OSCCs and normal samples analyzed. As these two markers have already been related to the mechanism of metastasis and relapses in cancer, its expression determination could help to understand the aggressive and invasive nature of OSCC. |
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Stem cell markers expression evaluation in Oral Squamous Cell CarcinomaEvaluación de la expresión de marcadores de células madre en el Carcinoma de Células Escamosas OralAvaliação da expressão de marcadores de células-tronco no Carcinoma de Células Escamosas OralCarcinoma de células escamosas orales, células madre, Nestin, NanogCarcinoma de células escamosas oralesCélulas madreNestinNanog.Carcinoma espinocelular oralCélulas-troncoNestinNanog.Oral squamous cell carcinoma, stem cells, Nestin, NanogOral squamous cell carcinomaStem cellsNestinNanog. Oral carcinogenesis is a highly complex process. Molecular studies demonstrate that some oral cancers progress from pre-neoplastic lesions. Cancer stem cells (CSCs), a small cellular group within the tumor, have currently received much attention by its self-renewal and tumor differentiation ability. It is considered that CSCs may be responsible for the relapse and metastasis of cancer. CSCs have been reported to express common embryonic stem cells markers such as Nestin and Nanog. The aim of the present study was to identify the presence of Nestin and Nanog markers in patients with oral squamous cell carcinoma (OSCC). Nine samples were collected from patients diagnosed with OSCC and two healthy oral mucosa were used as control. The markers expression in nine OSCC was determined by end-point PCR. The OSCC population analyzed was mostly composed of black and white males, with a mean age of 70.6 years, that reported alcohol and tobacco consumption. Nestin and Nanog stem cell markers were expressed in all OSCCs and normal samples analyzed. As these two markers have already been related to the mechanism of metastasis and relapses in cancer, its expression determination could help to understand the aggressive and invasive nature of OSCC.La carcinogénesis oral es un proceso muy complejo. Estudios moleculares demuestran que algunos cánceres orales progresan a partir de lesiones pre-neoplásicas. Las células madre cancerosas (CSCs), un pequeño grupo celular dentro del tumor, actualmente han recibido mucha atención por su capacidad de auto-renovación y diferenciación tumoral. Se considera que las CSCs pueden ser responsables de la recaída y metástasis del cáncer. Se ha informado de que las CSCs expresan marcadores comunes de células madre embrionarias como Nestin y Nanog. El objetivo del presente estudio fue identificar la presencia de marcadores de Nestin y Nanog en pacientes con carcinoma de células escamosas orales (OSCC). Se recogieron nueve muestras de pacientes con diagnóstico de OSCC y se utilizaron como control dos mucosas orales sanas. La expresión de los marcadores en nueve OSCC se determinó mediante PCR de punto final. La población OSCC analizada se componía principalmente de varones negros y blancos, con una edad media de 70,6 años, que reportaron consumo de alcohol y tabaco. Los marcadores de células madre Nestin y Nanog se expresaron en todos los OSCCs y muestras normales analizadas. Como estos dos marcadores ya se han relacionado con el mecanismo de metástasis y recaídas en el cáncer, su determinación de la expresión podría ayudar a entender la naturaleza agresiva e invasiva de OSCC.A carcinogênese oral é um processo altamente complexo. Estudos moleculares demonstram que alguns cânceres orais progridem a partir de lesões pré-neoplásicas. As células estaminais do cancro (Cscs), um grupo celular pequeno dentro do tumor, receberam atualmente muita atenção por sua capacidade auto replicante e da diferenciação do tumor. Considera-se que as Cscs podem ser responsáveis pela recidiva e metástase do câncer. Os Cscs foram relatados para expressar marcadores comuns das células estaminais embrionárias tais como Nestin e Nanog. O objetivo do presente estudo foi identificar a presença de marcadores de Nestin e Nanog em pacientes com carcinoma de células escamoso oral (CCEO). Foram coletadas nove amostras de pacientes diagnosticados com CCEO de boca e duas mucosas orais sadias foram utilizadas como controle. An expressão dos marcadores em nove CCEO foi determinada por PCR em tempo real. A população de CCEO analisada foi majoritariamente composta por homens negros e brancos, com idade média de 70,6 anos, que referiram consumo de álcool e tabaco. Marcadores de células-tronco de Nestin e Nanog foram expressos em todos os CCEO e amostras normais analisadas. Como esses dois marcadpres já foram relacionados ao mecanismo de metástase e recidivas no cancer, sua determinação de expressão poderia ajudar a entender a natureza agressiva e invasiva do CCEO.Research, Society and Development2021-10-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2084010.33448/rsd-v10i13.20840Research, Society and Development; Vol. 10 No. 13; e314101320840Research, Society and Development; Vol. 10 Núm. 13; e314101320840Research, Society and Development; v. 10 n. 13; e3141013208402525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/20840/18954Copyright (c) 2021 Julio Cesar Ramos Cadilho; Silvia Maria de Carvalho Lyra; Ana Beatriz Machado Lima; Carina Maciel da Silva Boghossian; Cláudia Maria Pereirahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCadilho, Julio Cesar Ramos Lyra, Silvia Maria de CarvalhoLima, Ana Beatriz MachadoBoghossian, Carina Maciel da SilvaPereira, Cláudia Maria2021-11-21T18:26:28Zoai:ojs.pkp.sfu.ca:article/20840Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:40:22.309617Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Stem cell markers expression evaluation in Oral Squamous Cell Carcinoma Evaluación de la expresión de marcadores de células madre en el Carcinoma de Células Escamosas Oral Avaliação da expressão de marcadores de células-tronco no Carcinoma de Células Escamosas Oral |
title |
Stem cell markers expression evaluation in Oral Squamous Cell Carcinoma |
spellingShingle |
Stem cell markers expression evaluation in Oral Squamous Cell Carcinoma Cadilho, Julio Cesar Ramos Carcinoma de células escamosas orales, células madre, Nestin, Nanog Carcinoma de células escamosas orales Células madre Nestin Nanog. Carcinoma espinocelular oral Células-tronco Nestin Nanog. Oral squamous cell carcinoma, stem cells, Nestin, Nanog Oral squamous cell carcinoma Stem cells Nestin Nanog. |
title_short |
Stem cell markers expression evaluation in Oral Squamous Cell Carcinoma |
title_full |
Stem cell markers expression evaluation in Oral Squamous Cell Carcinoma |
title_fullStr |
Stem cell markers expression evaluation in Oral Squamous Cell Carcinoma |
title_full_unstemmed |
Stem cell markers expression evaluation in Oral Squamous Cell Carcinoma |
title_sort |
Stem cell markers expression evaluation in Oral Squamous Cell Carcinoma |
author |
Cadilho, Julio Cesar Ramos |
author_facet |
Cadilho, Julio Cesar Ramos Lyra, Silvia Maria de Carvalho Lima, Ana Beatriz Machado Boghossian, Carina Maciel da Silva Pereira, Cláudia Maria |
author_role |
author |
author2 |
Lyra, Silvia Maria de Carvalho Lima, Ana Beatriz Machado Boghossian, Carina Maciel da Silva Pereira, Cláudia Maria |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Cadilho, Julio Cesar Ramos Lyra, Silvia Maria de Carvalho Lima, Ana Beatriz Machado Boghossian, Carina Maciel da Silva Pereira, Cláudia Maria |
dc.subject.por.fl_str_mv |
Carcinoma de células escamosas orales, células madre, Nestin, Nanog Carcinoma de células escamosas orales Células madre Nestin Nanog. Carcinoma espinocelular oral Células-tronco Nestin Nanog. Oral squamous cell carcinoma, stem cells, Nestin, Nanog Oral squamous cell carcinoma Stem cells Nestin Nanog. |
topic |
Carcinoma de células escamosas orales, células madre, Nestin, Nanog Carcinoma de células escamosas orales Células madre Nestin Nanog. Carcinoma espinocelular oral Células-tronco Nestin Nanog. Oral squamous cell carcinoma, stem cells, Nestin, Nanog Oral squamous cell carcinoma Stem cells Nestin Nanog. |
description |
Oral carcinogenesis is a highly complex process. Molecular studies demonstrate that some oral cancers progress from pre-neoplastic lesions. Cancer stem cells (CSCs), a small cellular group within the tumor, have currently received much attention by its self-renewal and tumor differentiation ability. It is considered that CSCs may be responsible for the relapse and metastasis of cancer. CSCs have been reported to express common embryonic stem cells markers such as Nestin and Nanog. The aim of the present study was to identify the presence of Nestin and Nanog markers in patients with oral squamous cell carcinoma (OSCC). Nine samples were collected from patients diagnosed with OSCC and two healthy oral mucosa were used as control. The markers expression in nine OSCC was determined by end-point PCR. The OSCC population analyzed was mostly composed of black and white males, with a mean age of 70.6 years, that reported alcohol and tobacco consumption. Nestin and Nanog stem cell markers were expressed in all OSCCs and normal samples analyzed. As these two markers have already been related to the mechanism of metastasis and relapses in cancer, its expression determination could help to understand the aggressive and invasive nature of OSCC. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-14 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/20840 10.33448/rsd-v10i13.20840 |
url |
https://rsdjournal.org/index.php/rsd/article/view/20840 |
identifier_str_mv |
10.33448/rsd-v10i13.20840 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/20840/18954 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 13; e314101320840 Research, Society and Development; Vol. 10 Núm. 13; e314101320840 Research, Society and Development; v. 10 n. 13; e314101320840 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
_version_ |
1797052756521910272 |