The effects of thermal and ethanolic stress in industrial strains of Saccharomyces cerevisiae
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/9091 |
Resumo: | Saccharomyces cerevisiae is exceptional microorganisms used in biotechnological processes, mainly in the ethanol production chain. Studies of the cellular responses of industrial yeasts under ethanolic and thermal stress in an association are still incipient. This study aimed to evaluate the action of thermal and ethanolic stress in industrial strains of Saccharomyces cerevisiae under different temperatures and concentrations of ethanol, to understand whether these factors influence ethanol production. For cytotoxicity and genotoxicity tests, yeasts were grown in 2% YPD medium incubated for 10 hours at 250 rpm. After growth, the samples were grown in sugarcane juice in concentrations of 5, 10 and 15% ethanol and incubated at 30 and 40 ºC. In Petri dishes containing the solid medium YPD 2% the yeasts were dripped and incubated for 72 hours the cytotoxic action was analyzed by cell growth and genotoxicity through the comet assay and ethanol production by gas chromatography. Cell growth occurred in all conditions, however, at 30 ºC there was inhibition in 10% (v v-1) of ethanol being potentiated in 15% (v v-1), at 40 ºC. The genotoxicity analysis showed an induction of DNA damage in yeasts, however, the FLE yeast was the one with the highest DNA damage index. The yeast Pedra-2 was more tolerant and produced more ethanol, showing to be a tolerant strain concerning the analyzed fermentative interferents. |
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The effects of thermal and ethanolic stress in industrial strains of Saccharomyces cerevisiaeLos efectos del estrés térmico y etanólico en cepas industriales de Saccharomyces cerevisiaeOs efeitos do estresse térmico e etanólico em linhagens industriais de Saccharomyces cerevisiaeFermentationYeastsStress condition.FermentaciónLevadurasCondición de estrés.FermentaçãoLevedurasCondição de estresse.Saccharomyces cerevisiae is exceptional microorganisms used in biotechnological processes, mainly in the ethanol production chain. Studies of the cellular responses of industrial yeasts under ethanolic and thermal stress in an association are still incipient. This study aimed to evaluate the action of thermal and ethanolic stress in industrial strains of Saccharomyces cerevisiae under different temperatures and concentrations of ethanol, to understand whether these factors influence ethanol production. For cytotoxicity and genotoxicity tests, yeasts were grown in 2% YPD medium incubated for 10 hours at 250 rpm. After growth, the samples were grown in sugarcane juice in concentrations of 5, 10 and 15% ethanol and incubated at 30 and 40 ºC. In Petri dishes containing the solid medium YPD 2% the yeasts were dripped and incubated for 72 hours the cytotoxic action was analyzed by cell growth and genotoxicity through the comet assay and ethanol production by gas chromatography. Cell growth occurred in all conditions, however, at 30 ºC there was inhibition in 10% (v v-1) of ethanol being potentiated in 15% (v v-1), at 40 ºC. The genotoxicity analysis showed an induction of DNA damage in yeasts, however, the FLE yeast was the one with the highest DNA damage index. The yeast Pedra-2 was more tolerant and produced more ethanol, showing to be a tolerant strain concerning the analyzed fermentative interferents.Saccharomyces cerevisiae son microorganismos excepcionales utilizados en procesos biotecnológicos, principalmente en la producción de etanol. Estudios de las respuestas celulares de levaduras industriales bajo estrés etanólico y térmico en asociación son todavía incipientes. El objetivo fue evaluar la acción del estrés térmico y etanólico en cepas industriales de Saccharomyces cerevisiae a diferentes temperaturas y concentraciones de etanol, con el fin de comprender si estos factores influyen en la producción de etanol. Para las pruebas de citotoxicidad y genotoxicidad, las levaduras se cultivaron en medio YPD al 2% incubados durante 10 horas a 250 rpm. Las muestras se cultivaron en jugo de caña de azúcar en 5, 10 y 15% de etanol y se incubaron a 30 y 40 ºC. En placas de Petri con el medio sólido YPD 2% se gotearon y se incubaron durante 72 horas, se analizó la acción citotóxica por crecimiento celular y genotoxicidad mediante el ensayo cometa y la producción de etanol mediante cromatografía de gases. El crecimiento celular ocurrió en todas las condiciones, a 30 ºC hubo inhibición en 10% (v v-1) de etanol siendo potenciado en 15% (v v-1) a 40 ºC. El análisis de genotoxicidad mostró una inducción de daño en el ADN en las levaduras. La levadura FLE fue la que presentó el mayor índice de daño en el ADN. La Pe-2 fue más tolerante y produjo más etanol, demostrando ser una cepa tolerante en relación a los interferentes fermentativos analizados.Saccharomyces cerevisiae são microrganismos excepcionais utilizadas nos processos biotecnológicos, principalmente na cadeia produtiva do etanol. Estudos das respostas celulares das leveduras industriais sob estresse etanólico e térmico em associação ainda são incipientes. Este estudo objetivou avaliar a ação do estresse térmico e etanólico em linhagens industriais de Saccharomyces cerevisiae sob diferentes temperaturas e concentrações de etanol, visando entender se estes fatores influenciam na produção de etanol. Para os testes de citotoxicidade e genotoxicidade as leveduras foram crescidas no meio YPD 2% incubadas por 10 horas a 250 rpm. Após crescimento as amostras foram cultivadas em caldo de cana nas concentrações de 5, 10 e 15% de etanol e incubadas a 30 e 40 ºC. Em placas de Petri contendo o meio sólido YPD 2% foram gotejadas as leveduras e incubadas por 72 horas. A ação citotóxica foi analisada pelo crescimento celular a genotoxicidade por meio do ensaio cometa e a produção de etanol por cromatografia gasosa. Ocorreu crescimento celular em todas as condições, contudo, em 30 °C houve inibição em 10% (v v-1) de etanol sendo potencializada em 15% (v v-1) a 40 ºC. A análise de genotoxicidade evidenciou indução de danos ao DNA das leveduras, porém a levedura FLE foi quem apresentou maior índice de lesões ao DNA. A levedura Pedra-2 foi mais tolerante e produziu mais etanol, mostrando ser uma linhagem tolerante em relação aos interferentes fermentativos analisados.Research, Society and Development2020-10-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/909110.33448/rsd-v9i10.9091Research, Society and Development; Vol. 9 No. 10; e6819109091Research, Society and Development; Vol. 9 Núm. 10; e6819109091Research, Society and Development; v. 9 n. 10; e68191090912525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/9091/8212Copyright (c) 2020 Larissa Pires Mueller; Maria do Socorro Mascarenhas Santos; Claudia Andrea Lima Cardoso; Margareth Batistotehttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessMueller, Larissa Pires Santos, Maria do Socorro Mascarenhas Cardoso, Claudia Andrea Lima Batistote, Margareth2020-10-31T12:03:23Zoai:ojs.pkp.sfu.ca:article/9091Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:31:25.422751Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
The effects of thermal and ethanolic stress in industrial strains of Saccharomyces cerevisiae Los efectos del estrés térmico y etanólico en cepas industriales de Saccharomyces cerevisiae Os efeitos do estresse térmico e etanólico em linhagens industriais de Saccharomyces cerevisiae |
title |
The effects of thermal and ethanolic stress in industrial strains of Saccharomyces cerevisiae |
spellingShingle |
The effects of thermal and ethanolic stress in industrial strains of Saccharomyces cerevisiae Mueller, Larissa Pires Fermentation Yeasts Stress condition. Fermentación Levaduras Condición de estrés. Fermentação Leveduras Condição de estresse. |
title_short |
The effects of thermal and ethanolic stress in industrial strains of Saccharomyces cerevisiae |
title_full |
The effects of thermal and ethanolic stress in industrial strains of Saccharomyces cerevisiae |
title_fullStr |
The effects of thermal and ethanolic stress in industrial strains of Saccharomyces cerevisiae |
title_full_unstemmed |
The effects of thermal and ethanolic stress in industrial strains of Saccharomyces cerevisiae |
title_sort |
The effects of thermal and ethanolic stress in industrial strains of Saccharomyces cerevisiae |
author |
Mueller, Larissa Pires |
author_facet |
Mueller, Larissa Pires Santos, Maria do Socorro Mascarenhas Cardoso, Claudia Andrea Lima Batistote, Margareth |
author_role |
author |
author2 |
Santos, Maria do Socorro Mascarenhas Cardoso, Claudia Andrea Lima Batistote, Margareth |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Mueller, Larissa Pires Santos, Maria do Socorro Mascarenhas Cardoso, Claudia Andrea Lima Batistote, Margareth |
dc.subject.por.fl_str_mv |
Fermentation Yeasts Stress condition. Fermentación Levaduras Condición de estrés. Fermentação Leveduras Condição de estresse. |
topic |
Fermentation Yeasts Stress condition. Fermentación Levaduras Condición de estrés. Fermentação Leveduras Condição de estresse. |
description |
Saccharomyces cerevisiae is exceptional microorganisms used in biotechnological processes, mainly in the ethanol production chain. Studies of the cellular responses of industrial yeasts under ethanolic and thermal stress in an association are still incipient. This study aimed to evaluate the action of thermal and ethanolic stress in industrial strains of Saccharomyces cerevisiae under different temperatures and concentrations of ethanol, to understand whether these factors influence ethanol production. For cytotoxicity and genotoxicity tests, yeasts were grown in 2% YPD medium incubated for 10 hours at 250 rpm. After growth, the samples were grown in sugarcane juice in concentrations of 5, 10 and 15% ethanol and incubated at 30 and 40 ºC. In Petri dishes containing the solid medium YPD 2% the yeasts were dripped and incubated for 72 hours the cytotoxic action was analyzed by cell growth and genotoxicity through the comet assay and ethanol production by gas chromatography. Cell growth occurred in all conditions, however, at 30 ºC there was inhibition in 10% (v v-1) of ethanol being potentiated in 15% (v v-1), at 40 ºC. The genotoxicity analysis showed an induction of DNA damage in yeasts, however, the FLE yeast was the one with the highest DNA damage index. The yeast Pedra-2 was more tolerant and produced more ethanol, showing to be a tolerant strain concerning the analyzed fermentative interferents. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-10-14 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/9091 10.33448/rsd-v9i10.9091 |
url |
https://rsdjournal.org/index.php/rsd/article/view/9091 |
identifier_str_mv |
10.33448/rsd-v9i10.9091 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/9091/8212 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 9 No. 10; e6819109091 Research, Society and Development; Vol. 9 Núm. 10; e6819109091 Research, Society and Development; v. 9 n. 10; e6819109091 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
_version_ |
1797052661401387008 |