Quantification of animal species using quantitative real-time PCR (qPCR) to verify fraud in meat products
Autor(a) principal: | |
---|---|
Data de Publicação: | 2023 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/38972 |
Resumo: | Food labeling is fundamental to avoid eating a specific kind of meat for religious and cultural reasons, but mainly to avoid fraud with the purpose of obtaining financial advantages. The real-time qPCR method is commonly used to quantify DNA in a given sample by relative quantification (concentration of specific target DNA over concentration of endogenous DNA). In studies that use qPCR to quantify contaminating species, normalization is performed using a single-copy reference gene, such as myostatin, present in most mammals and birds. This study aimed to standardize the real-time qPCR technique to obtain relative quantitative results in rigged beef. For method standardization, the limit of detection (LD) resulted in a Cq fluorescence signal of 36.80 cycles in at least 9 out of 10 repetitions (90%) at a concentration of 0.008% in horse and Cq of 36.60 cycles in at least 7 of 10 repetitions (70%) at a concentration of 0.0005% in chicken. The lowest concentration at which the relative standard deviation (RSD) was ≤25% was between 0.125% and 0.031% for both horse and chicken. Even using standard quantitation curves containing 50% meat blends or 100% pure target meat, curve slope, amplification efficiency, and linear correlation were within recommended acceptance criteria. The test is sensitive and an alternative in the routine inspection of products for both human and animal consumption. |
id |
UNIFEI_7eac08e1943919c3a8d619b2b283341e |
---|---|
oai_identifier_str |
oai:ojs.pkp.sfu.ca:article/38972 |
network_acronym_str |
UNIFEI |
network_name_str |
Research, Society and Development |
repository_id_str |
|
spelling |
Quantification of animal species using quantitative real-time PCR (qPCR) to verify fraud in meat productsCuantificación de especies animales mediante PCR cuantitativa en tiempo real (qPCR) para verificar el fraude en productos cárnicosQuantificação de espécies animais utilizando PCR em tempo real quantitativa (qPCR) para verificar fraudes em produtos cárneos Real-time qPCRValidationBeef and chicken meatMyostatin.qPCR en tiempo realValidaciónCarne de res y polloMiostatina.Real-time qPCRValidaçãoCarne bovina e frangoMiostatina. Food labeling is fundamental to avoid eating a specific kind of meat for religious and cultural reasons, but mainly to avoid fraud with the purpose of obtaining financial advantages. The real-time qPCR method is commonly used to quantify DNA in a given sample by relative quantification (concentration of specific target DNA over concentration of endogenous DNA). In studies that use qPCR to quantify contaminating species, normalization is performed using a single-copy reference gene, such as myostatin, present in most mammals and birds. This study aimed to standardize the real-time qPCR technique to obtain relative quantitative results in rigged beef. For method standardization, the limit of detection (LD) resulted in a Cq fluorescence signal of 36.80 cycles in at least 9 out of 10 repetitions (90%) at a concentration of 0.008% in horse and Cq of 36.60 cycles in at least 7 of 10 repetitions (70%) at a concentration of 0.0005% in chicken. The lowest concentration at which the relative standard deviation (RSD) was ≤25% was between 0.125% and 0.031% for both horse and chicken. Even using standard quantitation curves containing 50% meat blends or 100% pure target meat, curve slope, amplification efficiency, and linear correlation were within recommended acceptance criteria. The test is sensitive and an alternative in the routine inspection of products for both human and animal consumption.El etiquetado de los alimentos es fundamental para evitar el consumo de un determinado tipo de carne por motivos religiosos y culturales, pero principalmente para evitar fraudes con el fin de obtener ventajas económicas. El método de qPCR en tiempo real se usa comúnmente para cuantificar el ADN en una muestra determinada mediante la cuantificación relativa (concentración de ADN diana específica sobre la concentración de ADN endógeno). En estudios que usan qPCR para cuantificar especies contaminantes, la normalización se realiza usando un gen de referencia de una sola copia, como la miostatina, presente en la mayoría de los mamíferos y aves. Este estudio tuvo como objetivo estandarizar la técnica de qPCR en tiempo real para obtener resultados cuantitativos relativos en carne de res manipulada. Para la estandarización del método, el límite de detección (LD) resultó en una señal de fluorescencia Cq de 36,80 ciclos en al menos 9 de 10 repeticiones (90%) a una concentración de 0,008% en caballo y Cq de 36,60 ciclos en al menos 7 de 10 repeticiones (70%) a una concentración de 0,0005% en pollo. La concentración más baja a la que la desviación estándar relativa (RSD) fue ≤25% estuvo entre 0,125% y 0,031% tanto para caballos como para pollos. Incluso usando curvas de cuantificación estándar que contenían 50 % de mezclas de carne o 100 % de carne objetivo pura, la pendiente de la curva, la eficiencia de amplificación y la correlación lineal estuvieron dentro de los criterios de aceptación recomendados. La prueba es sensible y una alternativa en la inspección de rutina de productos tanto para consumo humano como animal.A rotulagem de alimentos é fundamental para evitar a ingestão de uma espécie de carne específica por motivos religiosos e razões culturais, mas, principalmente, para evitar fraudes com o propósito de obter vantagens financeiras. O método real-time qPCR é comumente utilizado para quantificar o DNA em uma determinada amostra por quantificação relativa (concentração do DNA alvo específico sobre a concentração do DNA endógeno). Em estudos que usam qPCR para quantificar espécies contaminantes, a normalização é realizada usando um gene de referência de cópia única, como a miostatina, presente na maioria dos mamíferos e das aves. Esse estudo teve por objetivo padronizar a técnica real-time qPCR para a obtenção de resultados quantitativos relativos em carnes bovinas fraudadas. Para a padronização do método, o limite de detecção (LD) resultou em um sinal de fluorescência Cq de 36.80 ciclos em pelo menos 9 de 10 repetições (90%) na concentração de 0,008% em cavalo e Cq de 36.60 ciclos em pelo menos 7 de 10 repetições (70%) na concentração de 0,0005% em frango. A concentração mais baixa em que o desvio padrão relativo (RSD) foi ≤25% estava entre 0,125% e 0,031% tanto para cavalo quanto para frango. Mesmo usando curvas padrões de quantificação contendo 50% de misturas de carnes ou 100% de carne pura do alvo, a inclinação da curva, a eficiência de amplificação e a correlação linear estiveram dentro do critério de aceitação recomendado. O ensaio é sensível e uma alternativa na rotina de fiscalização de produtos tanto para alimentação humana quanto animal.Research, Society and Development2023-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/3897210.33448/rsd-v12i1.38972Research, Society and Development; Vol. 12 No. 1; e1512138972Research, Society and Development; Vol. 12 Núm. 1; e1512138972Research, Society and Development; v. 12 n. 1; e15121389722525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/38972/32332Copyright (c) 2023 Hans Fröder; Eléia Righihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessFröder, HansRighi, Eléia2023-01-13T10:30:42Zoai:ojs.pkp.sfu.ca:article/38972Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2023-01-13T10:30:42Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Quantification of animal species using quantitative real-time PCR (qPCR) to verify fraud in meat products Cuantificación de especies animales mediante PCR cuantitativa en tiempo real (qPCR) para verificar el fraude en productos cárnicos Quantificação de espécies animais utilizando PCR em tempo real quantitativa (qPCR) para verificar fraudes em produtos cárneos |
title |
Quantification of animal species using quantitative real-time PCR (qPCR) to verify fraud in meat products |
spellingShingle |
Quantification of animal species using quantitative real-time PCR (qPCR) to verify fraud in meat products Fröder, Hans Real-time qPCR Validation Beef and chicken meat Myostatin. qPCR en tiempo real Validación Carne de res y pollo Miostatina. Real-time qPCR Validação Carne bovina e frango Miostatina. |
title_short |
Quantification of animal species using quantitative real-time PCR (qPCR) to verify fraud in meat products |
title_full |
Quantification of animal species using quantitative real-time PCR (qPCR) to verify fraud in meat products |
title_fullStr |
Quantification of animal species using quantitative real-time PCR (qPCR) to verify fraud in meat products |
title_full_unstemmed |
Quantification of animal species using quantitative real-time PCR (qPCR) to verify fraud in meat products |
title_sort |
Quantification of animal species using quantitative real-time PCR (qPCR) to verify fraud in meat products |
author |
Fröder, Hans |
author_facet |
Fröder, Hans Righi, Eléia |
author_role |
author |
author2 |
Righi, Eléia |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Fröder, Hans Righi, Eléia |
dc.subject.por.fl_str_mv |
Real-time qPCR Validation Beef and chicken meat Myostatin. qPCR en tiempo real Validación Carne de res y pollo Miostatina. Real-time qPCR Validação Carne bovina e frango Miostatina. |
topic |
Real-time qPCR Validation Beef and chicken meat Myostatin. qPCR en tiempo real Validación Carne de res y pollo Miostatina. Real-time qPCR Validação Carne bovina e frango Miostatina. |
description |
Food labeling is fundamental to avoid eating a specific kind of meat for religious and cultural reasons, but mainly to avoid fraud with the purpose of obtaining financial advantages. The real-time qPCR method is commonly used to quantify DNA in a given sample by relative quantification (concentration of specific target DNA over concentration of endogenous DNA). In studies that use qPCR to quantify contaminating species, normalization is performed using a single-copy reference gene, such as myostatin, present in most mammals and birds. This study aimed to standardize the real-time qPCR technique to obtain relative quantitative results in rigged beef. For method standardization, the limit of detection (LD) resulted in a Cq fluorescence signal of 36.80 cycles in at least 9 out of 10 repetitions (90%) at a concentration of 0.008% in horse and Cq of 36.60 cycles in at least 7 of 10 repetitions (70%) at a concentration of 0.0005% in chicken. The lowest concentration at which the relative standard deviation (RSD) was ≤25% was between 0.125% and 0.031% for both horse and chicken. Even using standard quantitation curves containing 50% meat blends or 100% pure target meat, curve slope, amplification efficiency, and linear correlation were within recommended acceptance criteria. The test is sensitive and an alternative in the routine inspection of products for both human and animal consumption. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/38972 10.33448/rsd-v12i1.38972 |
url |
https://rsdjournal.org/index.php/rsd/article/view/38972 |
identifier_str_mv |
10.33448/rsd-v12i1.38972 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/38972/32332 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 Hans Fröder; Eléia Righi https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 Hans Fröder; Eléia Righi https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 12 No. 1; e1512138972 Research, Society and Development; Vol. 12 Núm. 1; e1512138972 Research, Society and Development; v. 12 n. 1; e1512138972 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
_version_ |
1797052614484951040 |