The Nobel Prize in Physiology 1977: A literature review of the Radioimmunoassay Technique
Autor(a) principal: | |
---|---|
Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/42090 |
Resumo: | The first study about radioimmunoassay was published in 1960 by 2 scientists, who created this new method to quantify human insulin, at a time when studies on diabetes and its pathophysiology were beginning to stand out. Years later, due to its strong relevance to the scientific world, such work was awarded the Nobel Prize in Physiology in 1977. Thus, the present study aims to expose the historical context of the discovery, the initial studies on diseases whose immunological origin was still unknown, the beginning of radioimmunoassay techniques and their potential benefits and effects for science. Therefore, a narrative review of the literature was carried out using the PubMed, Scielo and Google Scholar databases in order to find the theoretical bases that underlie the objective of the present study. The study revealed that through the use of radioisotopes for insulin labeling and the use of antibodies from animals, it was possible to quantify hormones at low blood concentrations, based on the graphic of the proportion between free marked insulin and insulin combined with antibodies. Such discovery was important to understand the pathophysiology of many diseases, although on the other hand it presents its risks because it involves radioactive material and because it is now in disuse due to the creation of methods such as ELISA. It is concluded that due to the historical and scientific importance of the RIA, this method proved to be worthy of the most renowned scientific award in the world. |
id |
UNIFEI_84296752d7f57d20d9943fb92c653375 |
---|---|
oai_identifier_str |
oai:ojs.pkp.sfu.ca:article/42090 |
network_acronym_str |
UNIFEI |
network_name_str |
Research, Society and Development |
repository_id_str |
|
spelling |
The Nobel Prize in Physiology 1977: A literature review of the Radioimmunoassay TechniqueEl Premio Nobel de Fisiología 1977: Una revisión de la literatura acerca de la Técnica de RadioinmunoensayoO Prêmio Nobel de Fisiologia de 1977: Uma revisão da literatura sobre a Técnica de RadioimunoensaioRadioimmunoassayNobelPhysiologyInsulin.RadioinmunoensayoNobelFisiologíaInsulina.RadioimunoensaioNobelFisiologiaInsulina.The first study about radioimmunoassay was published in 1960 by 2 scientists, who created this new method to quantify human insulin, at a time when studies on diabetes and its pathophysiology were beginning to stand out. Years later, due to its strong relevance to the scientific world, such work was awarded the Nobel Prize in Physiology in 1977. Thus, the present study aims to expose the historical context of the discovery, the initial studies on diseases whose immunological origin was still unknown, the beginning of radioimmunoassay techniques and their potential benefits and effects for science. Therefore, a narrative review of the literature was carried out using the PubMed, Scielo and Google Scholar databases in order to find the theoretical bases that underlie the objective of the present study. The study revealed that through the use of radioisotopes for insulin labeling and the use of antibodies from animals, it was possible to quantify hormones at low blood concentrations, based on the graphic of the proportion between free marked insulin and insulin combined with antibodies. Such discovery was important to understand the pathophysiology of many diseases, although on the other hand it presents its risks because it involves radioactive material and because it is now in disuse due to the creation of methods such as ELISA. It is concluded that due to the historical and scientific importance of the RIA, this method proved to be worthy of the most renowned scientific award in the world.El primer estudio sobre radioinmunoensayo fue publicado en 1960 por 2 científicos, que crearon este nuevo método para cuantificar la insulina humana, en un momento en que empezaban a destacar los estudios sobre la diabetes y su fisiopatología. Años más tarde, por su gran relevancia para la comunidad científica, este trabajo fue galardonado con el Premio Nobel de Fisiología en 1977. Así, el presente estudio pretende exponer el contexto histórico del descubrimiento, los estudios iniciales sobre enfermedades cuyo origen inmunológico aún era desconocido, el comienzo de las técnicas de radioinmunoensayo y sus posibles beneficios y efectos para la ciencia. Así, se realizó una revisión narrativa de la literatura en las bases de datos PubMed, Scielo y Google Scholar, con el fin de encontrar las bases teóricas que sustentan el objetivo del presente estudio. El estudio reveló que mediante el uso de radioisótopos para el marcaje de insulina y el uso de anticuerpos de animales, fue posible cuantificar hormonas a bajas concentraciones en sangre, con base en el gráfico de la proporción entre insulina libre marcada e insulina combinada con anticuerpos. Esta descoberta ayudó a comprender la fisiopatología de muchas enfermedades, aunque por otro lado presenta sus riesgos por tratarse de material radiactivo y por estar en desuso debido a la creación de métodos como ELISA. Se concluye que debido a la importancia histórica y científica del RIA, este método resultó ser merecedor del premio científico más renombrado del mundo.O primeiro estudo sobre radioimunoensaio (RIA) foi publicado em 1960 por 2 cientistas, que criaram este novo método para quantificar a insulina humana, em uma época em que os estudos sobre a diabetes e a sua fisiopatologia começavam a se destacar. Anos mais tarde, por sua grande relevância para o meio científico, tal trabalho foi agraciado com o Prêmio Nobel de Fisiologia em 1977. Assim, o presente estudo visa expor o contexto histórico da descoberta, os estudos iniciais sobre doenças cuja origem imunológica ainda era desconhecida, o início das técnicas de radioimunoensaio e seus potenciais benefícios e efeitos para a ciência. Para tanto, foi realizada uma revisão narrativa da literatura nas bases de dados PubMed, Scielo e Google Acadêmico, a fim de encontrar as bases teóricas que fundamentam o objetivo do presente estudo. O estudo revelou que através do uso de radioisótopos para marcação de insulina e o uso de anticorpos provenientes de animais, foi possível quantificar hormônios de baixas concentrações sanguíneas, baseado no gráfico da proporção entre insulina marcada livre e insulina combinada com anticorpos Essa invenção se tornou ferramenta no entendimento da fisiopatologia de muitas outras doenças, embora por outro lado apresenta seus riscos por envolver material radioativo e por hoje estar em desuso devido a criação de métodos como o ELISA. Conclui-se que devido a importância histórica e científica do RIA, tal método mostrou ser digno do prêmio de maior renome científico do mundo.Research, Society and Development2023-06-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/4209010.33448/rsd-v12i6.42090Research, Society and Development; Vol. 12 No. 6; e14712642090Research, Society and Development; Vol. 12 Núm. 6; e14712642090Research, Society and Development; v. 12 n. 6; e147126420902525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/42090/34115Copyright (c) 2023 Matheus dos Santos do Nascimento Carvalho; Adriano Santana de Luna Costa; Allan Vieira Almeida Santana; Rodrigo Santana de Luna Batista; Fernando Pinto de Mesquita Neto; Jefferson Cavalcante de Lima; Adriane Borges Cabralhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCarvalho, Matheus dos Santos do Nascimento Costa, Adriano Santana de Luna Santana, Allan Vieira Almeida Batista, Rodrigo Santana de Luna Mesquita Neto, Fernando Pinto de Lima, Jefferson Cavalcante de Cabral, Adriane Borges 2023-07-06T11:16:27Zoai:ojs.pkp.sfu.ca:article/42090Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2023-07-06T11:16:27Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
The Nobel Prize in Physiology 1977: A literature review of the Radioimmunoassay Technique El Premio Nobel de Fisiología 1977: Una revisión de la literatura acerca de la Técnica de Radioinmunoensayo O Prêmio Nobel de Fisiologia de 1977: Uma revisão da literatura sobre a Técnica de Radioimunoensaio |
title |
The Nobel Prize in Physiology 1977: A literature review of the Radioimmunoassay Technique |
spellingShingle |
The Nobel Prize in Physiology 1977: A literature review of the Radioimmunoassay Technique Carvalho, Matheus dos Santos do Nascimento Radioimmunoassay Nobel Physiology Insulin. Radioinmunoensayo Nobel Fisiología Insulina. Radioimunoensaio Nobel Fisiologia Insulina. |
title_short |
The Nobel Prize in Physiology 1977: A literature review of the Radioimmunoassay Technique |
title_full |
The Nobel Prize in Physiology 1977: A literature review of the Radioimmunoassay Technique |
title_fullStr |
The Nobel Prize in Physiology 1977: A literature review of the Radioimmunoassay Technique |
title_full_unstemmed |
The Nobel Prize in Physiology 1977: A literature review of the Radioimmunoassay Technique |
title_sort |
The Nobel Prize in Physiology 1977: A literature review of the Radioimmunoassay Technique |
author |
Carvalho, Matheus dos Santos do Nascimento |
author_facet |
Carvalho, Matheus dos Santos do Nascimento Costa, Adriano Santana de Luna Santana, Allan Vieira Almeida Batista, Rodrigo Santana de Luna Mesquita Neto, Fernando Pinto de Lima, Jefferson Cavalcante de Cabral, Adriane Borges |
author_role |
author |
author2 |
Costa, Adriano Santana de Luna Santana, Allan Vieira Almeida Batista, Rodrigo Santana de Luna Mesquita Neto, Fernando Pinto de Lima, Jefferson Cavalcante de Cabral, Adriane Borges |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Carvalho, Matheus dos Santos do Nascimento Costa, Adriano Santana de Luna Santana, Allan Vieira Almeida Batista, Rodrigo Santana de Luna Mesquita Neto, Fernando Pinto de Lima, Jefferson Cavalcante de Cabral, Adriane Borges |
dc.subject.por.fl_str_mv |
Radioimmunoassay Nobel Physiology Insulin. Radioinmunoensayo Nobel Fisiología Insulina. Radioimunoensaio Nobel Fisiologia Insulina. |
topic |
Radioimmunoassay Nobel Physiology Insulin. Radioinmunoensayo Nobel Fisiología Insulina. Radioimunoensaio Nobel Fisiologia Insulina. |
description |
The first study about radioimmunoassay was published in 1960 by 2 scientists, who created this new method to quantify human insulin, at a time when studies on diabetes and its pathophysiology were beginning to stand out. Years later, due to its strong relevance to the scientific world, such work was awarded the Nobel Prize in Physiology in 1977. Thus, the present study aims to expose the historical context of the discovery, the initial studies on diseases whose immunological origin was still unknown, the beginning of radioimmunoassay techniques and their potential benefits and effects for science. Therefore, a narrative review of the literature was carried out using the PubMed, Scielo and Google Scholar databases in order to find the theoretical bases that underlie the objective of the present study. The study revealed that through the use of radioisotopes for insulin labeling and the use of antibodies from animals, it was possible to quantify hormones at low blood concentrations, based on the graphic of the proportion between free marked insulin and insulin combined with antibodies. Such discovery was important to understand the pathophysiology of many diseases, although on the other hand it presents its risks because it involves radioactive material and because it is now in disuse due to the creation of methods such as ELISA. It is concluded that due to the historical and scientific importance of the RIA, this method proved to be worthy of the most renowned scientific award in the world. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-06-15 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/42090 10.33448/rsd-v12i6.42090 |
url |
https://rsdjournal.org/index.php/rsd/article/view/42090 |
identifier_str_mv |
10.33448/rsd-v12i6.42090 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/42090/34115 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 12 No. 6; e14712642090 Research, Society and Development; Vol. 12 Núm. 6; e14712642090 Research, Society and Development; v. 12 n. 6; e14712642090 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
_version_ |
1797052626170281984 |