In vitro biocompatibility analysis of two types of titanium surfaces treated by Electric Discharge Machining (EDM)

Detalhes bibliográficos
Autor(a) principal: Garcia, Rogério Ferreira
Data de Publicação: 2021
Outros Autores: Lima, Isabela Lemos de, Castro, Paloma Soares de, Goulart, Luiz Ricardo, Alonso-Goulart, Vivian, Rocha, Flaviana Soares, Raslan, Alberto Arnaldo, Filice , Letícia de Souza Castro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/17474
Resumo: The aim of the present work was to assess the biological viability of two titanium surfaces treated by electric discharge machining (EDM) using water or hydroxyapatite as modifying agents and compare them to a machined titanium surface without modifying agent as a control. The in vitro MTT, total protein, alkaline phosphatase and alizarin red assays and scanning electron microscopy were applied to analyze pre-osteoblastic MC3T3-E1 cells after 7, 14 and 21 days of cell culture on the titanium surfaces. The results showed the presence of cellular activity in all surfaces and the formation of bone matrix, with no discrepancy among the groups. All tested surfaces were capable of inducing bone formation. In the topographic analysis of the surface, EDM failed to modify the surface of the discs homogeneously. Thus, EDM is a low-cost, biocompatible technique that favors osseointegration, but which still needs to be improved.
id UNIFEI_88659fe3ccbda2fca4b93430e4b12906
oai_identifier_str oai:ojs.pkp.sfu.ca:article/17474
network_acronym_str UNIFEI
network_name_str Research, Society and Development
repository_id_str
spelling In vitro biocompatibility analysis of two types of titanium surfaces treated by Electric Discharge Machining (EDM) Análisis in vitro de biocompatibilidad de dos tipos de superficies de titanio tratadas mediante mecanizado por descarga eléctrica (EDM) Análise in vitro da biocompatibilidade de dois tipos de superfícies de titânio tratadas por descarga elétrica (EDM)Medicina regenerativaTitanioEDMHidroxiapatitaMC3T3-E1.Medicina regenerativaTitânioEDMHidroxiapatitaMC3T3-E1.Regenerative medicineTitaniumEDMHydroxyapatiteMC3T3-E1.The aim of the present work was to assess the biological viability of two titanium surfaces treated by electric discharge machining (EDM) using water or hydroxyapatite as modifying agents and compare them to a machined titanium surface without modifying agent as a control. The in vitro MTT, total protein, alkaline phosphatase and alizarin red assays and scanning electron microscopy were applied to analyze pre-osteoblastic MC3T3-E1 cells after 7, 14 and 21 days of cell culture on the titanium surfaces. The results showed the presence of cellular activity in all surfaces and the formation of bone matrix, with no discrepancy among the groups. All tested surfaces were capable of inducing bone formation. In the topographic analysis of the surface, EDM failed to modify the surface of the discs homogeneously. Thus, EDM is a low-cost, biocompatible technique that favors osseointegration, but which still needs to be improved.El objetivo del presente trabajo fue evaluar la viabilidad biológica de dos superficies de titanio tratadas mediante mecanizado por descarga eléctrica (EDM) utilizando agua o hidroxiapatita como agentes modificadores y comparándolas con una superficie mecanizada de titanio sin agente modificador como control. Se aplicaron ensayos in vitro de MTT, proteína total, fosfatasa alcalina y rojo de alizarina y microscopía electrónica de barrido para analizar células MC3T3-E1 preosteoblásticas después de 7, 14 y 21 días de cultivo celular en las superficies de titanio. Los resultados permitieron verificar la presencia de actividad celular en todas las superficies y la formación de matriz ósea, sin discrepancia entre los grupos. Todos los tipos de superficies testadas pueden inducir la formación de hueso. En el análisis topográfico de la superficie, el EDM no logró modificar la superficie de los discos de manera homogénea. Por tanto, la electroerosión es una técnica biocompatible de bajo coste que favorece la osteointegración, pero que todavía necesita ser mejorada.O objetivo do presente trabalho foi avaliar a biocompatibilidade de duas superfícies de titânio tratadas por descarga elétrica (EDM) utilizando água ou hidroxiapatita como agentes modificadores e comparando-as a uma superfície usinada de titânio sem agente modificador como controle. Foram realizados ensaios in vitro de MTT, proteína total, fosfatase alcalina e vermelho de alizarina, além de microscopia eletrônica de varredura para analisar o comportamento das células MC3T3-E1 pré-osteoblásticas após 7, 14 e 21 dias de cultivo celular nas superfícies de titânio. Os resultados permitiram verificar a presença de atividade celular em todas as superfícies e a formação de matriz óssea, não havendo discrepância entre os grupos. Todos as superfícies testadas foram capazes de induzir a formação óssea. Na análise topográfica da superfície, o EDM não conseguiu modificar a superfície dos discos de maneira homogênea. Assim, o EDM é uma técnica de baixo custo, biocompatível, que favorece a osteointegração, mas que ainda precisa ser aprimorada.Research, Society and Development2021-07-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1747410.33448/rsd-v10i8.17474Research, Society and Development; Vol. 10 No. 8; e44710817474Research, Society and Development; Vol. 10 Núm. 8; e44710817474Research, Society and Development; v. 10 n. 8; e447108174742525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/17474/15667Copyright (c) 2021 Rogério Ferreira Garcia; Isabela Lemos de Lima; Paloma Soares de Castro; Luiz Ricardo Goulart; Vivian Alonso-Goulart; Flaviana Soares Rocha; Alberto Arnaldo Raslan; Letícia de Souza Castro Filice https://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessGarcia, Rogério Ferreira Lima, Isabela Lemos de Castro, Paloma Soares de Goulart, Luiz Ricardo Alonso-Goulart, Vivian Rocha, Flaviana SoaresRaslan, Alberto Arnaldo Filice , Letícia de Souza Castro 2021-08-21T18:46:59Zoai:ojs.pkp.sfu.ca:article/17474Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:37:47.996500Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv In vitro biocompatibility analysis of two types of titanium surfaces treated by Electric Discharge Machining (EDM)
Análisis in vitro de biocompatibilidad de dos tipos de superficies de titanio tratadas mediante mecanizado por descarga eléctrica (EDM)
Análise in vitro da biocompatibilidade de dois tipos de superfícies de titânio tratadas por descarga elétrica (EDM)
title In vitro biocompatibility analysis of two types of titanium surfaces treated by Electric Discharge Machining (EDM)
spellingShingle In vitro biocompatibility analysis of two types of titanium surfaces treated by Electric Discharge Machining (EDM)
Garcia, Rogério Ferreira
Medicina regenerativa
Titanio
EDM
Hidroxiapatita
MC3T3-E1.
Medicina regenerativa
Titânio
EDM
Hidroxiapatita
MC3T3-E1.
Regenerative medicine
Titanium
EDM
Hydroxyapatite
MC3T3-E1.
title_short In vitro biocompatibility analysis of two types of titanium surfaces treated by Electric Discharge Machining (EDM)
title_full In vitro biocompatibility analysis of two types of titanium surfaces treated by Electric Discharge Machining (EDM)
title_fullStr In vitro biocompatibility analysis of two types of titanium surfaces treated by Electric Discharge Machining (EDM)
title_full_unstemmed In vitro biocompatibility analysis of two types of titanium surfaces treated by Electric Discharge Machining (EDM)
title_sort In vitro biocompatibility analysis of two types of titanium surfaces treated by Electric Discharge Machining (EDM)
author Garcia, Rogério Ferreira
author_facet Garcia, Rogério Ferreira
Lima, Isabela Lemos de
Castro, Paloma Soares de
Goulart, Luiz Ricardo
Alonso-Goulart, Vivian
Rocha, Flaviana Soares
Raslan, Alberto Arnaldo
Filice , Letícia de Souza Castro
author_role author
author2 Lima, Isabela Lemos de
Castro, Paloma Soares de
Goulart, Luiz Ricardo
Alonso-Goulart, Vivian
Rocha, Flaviana Soares
Raslan, Alberto Arnaldo
Filice , Letícia de Souza Castro
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Garcia, Rogério Ferreira
Lima, Isabela Lemos de
Castro, Paloma Soares de
Goulart, Luiz Ricardo
Alonso-Goulart, Vivian
Rocha, Flaviana Soares
Raslan, Alberto Arnaldo
Filice , Letícia de Souza Castro
dc.subject.por.fl_str_mv Medicina regenerativa
Titanio
EDM
Hidroxiapatita
MC3T3-E1.
Medicina regenerativa
Titânio
EDM
Hidroxiapatita
MC3T3-E1.
Regenerative medicine
Titanium
EDM
Hydroxyapatite
MC3T3-E1.
topic Medicina regenerativa
Titanio
EDM
Hidroxiapatita
MC3T3-E1.
Medicina regenerativa
Titânio
EDM
Hidroxiapatita
MC3T3-E1.
Regenerative medicine
Titanium
EDM
Hydroxyapatite
MC3T3-E1.
description The aim of the present work was to assess the biological viability of two titanium surfaces treated by electric discharge machining (EDM) using water or hydroxyapatite as modifying agents and compare them to a machined titanium surface without modifying agent as a control. The in vitro MTT, total protein, alkaline phosphatase and alizarin red assays and scanning electron microscopy were applied to analyze pre-osteoblastic MC3T3-E1 cells after 7, 14 and 21 days of cell culture on the titanium surfaces. The results showed the presence of cellular activity in all surfaces and the formation of bone matrix, with no discrepancy among the groups. All tested surfaces were capable of inducing bone formation. In the topographic analysis of the surface, EDM failed to modify the surface of the discs homogeneously. Thus, EDM is a low-cost, biocompatible technique that favors osseointegration, but which still needs to be improved.
publishDate 2021
dc.date.none.fl_str_mv 2021-07-15
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/17474
10.33448/rsd-v10i8.17474
url https://rsdjournal.org/index.php/rsd/article/view/17474
identifier_str_mv 10.33448/rsd-v10i8.17474
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/17474/15667
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 8; e44710817474
Research, Society and Development; Vol. 10 Núm. 8; e44710817474
Research, Society and Development; v. 10 n. 8; e44710817474
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052751689023488

Registros relacionados