Evaluation of the antifungal potential of Cymbopogon citratus essential oil in the control of the fungus Aspergillus brasiliensis
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/4697 |
Resumo: | The objective of this work was to evaluate the antifungal activity of the essential oil of Cymbopogon citratus in the control of Aspergillus brasiliensis in analysis in vitro and in stored corn kernels. Two in vitro tests were carried out. One of them, by diffusion in agar and the other by microdilution in wells. The concentrations used for the agar diffusion assay were 0.2; 0.4; 0.6; 0.8 and 1.0 µL mL-1. The concentrations used for the microdilution test were: 0.1; 0.2; 0.3; 0.4; 0.5; 0.6; 0.7; 0.8; 0.9; 1.0 and 1.2 µL mL-1. The mycelial growth was evaluated over time, using the Scott-Knott test at 5% significance. From the results obtained, it was verified that the application of the essential oil of Cymbopogon citratus interferes significantly in the fungal development of the species Aspergillus brasiliensis. The microdilution test in wells was used in a qualitative way, indicating absence of growth in doses from 0.8 µL mL-1. For the corn grain test, the essential oil dosages of 0.5; 0.8 and 1.0 µL mL-1 and were stored for 42 days. There was no significant difference in the different doses of essential oil over the storage period for this assay. |
id |
UNIFEI_8e20861833e82c5ad76c860631452d51 |
---|---|
oai_identifier_str |
oai:ojs.pkp.sfu.ca:article/4697 |
network_acronym_str |
UNIFEI |
network_name_str |
Research, Society and Development |
repository_id_str |
|
spelling |
Evaluation of the antifungal potential of Cymbopogon citratus essential oil in the control of the fungus Aspergillus brasiliensisEvaluación del potencial antifúngico del aceite esencial de Cymbopogon citratus en el control del hongo Aspergillus brasiliensisAvaliação do potencial antifúngico do óleo essencial de Cymbopogon citratus no controle do fungo Aspergillus brasiliensisCapim-limãoGrãos armazenadosFungicida natural.LemongrassStored grainsNatural fungicide.LimoncilloGranos almacenadosFungicida natural.The objective of this work was to evaluate the antifungal activity of the essential oil of Cymbopogon citratus in the control of Aspergillus brasiliensis in analysis in vitro and in stored corn kernels. Two in vitro tests were carried out. One of them, by diffusion in agar and the other by microdilution in wells. The concentrations used for the agar diffusion assay were 0.2; 0.4; 0.6; 0.8 and 1.0 µL mL-1. The concentrations used for the microdilution test were: 0.1; 0.2; 0.3; 0.4; 0.5; 0.6; 0.7; 0.8; 0.9; 1.0 and 1.2 µL mL-1. The mycelial growth was evaluated over time, using the Scott-Knott test at 5% significance. From the results obtained, it was verified that the application of the essential oil of Cymbopogon citratus interferes significantly in the fungal development of the species Aspergillus brasiliensis. The microdilution test in wells was used in a qualitative way, indicating absence of growth in doses from 0.8 µL mL-1. For the corn grain test, the essential oil dosages of 0.5; 0.8 and 1.0 µL mL-1 and were stored for 42 days. There was no significant difference in the different doses of essential oil over the storage period for this assay.El objetivo de este trabajo fue evaluar la actividad antifúngica del aceite esencial de Cymbopogon citratus en el control de Aspergillus brasiliensis en análisis in vitro y en granos de maíz almacenados. Se realizaron dos pruebas in vitro, una por difusión en agar y la otra por microdilución en pozos. Las concentraciones utilizadas para la prueba de difusión en agar fueron 0.2; 0.4; 0.6; 0.8 y 1.0 µL mL-1. Para la prueba de microdilución en pozos, se usaron dosis de 0.1; 0.2; 0.3; 0.4; 0,5; 0.6; 0,7; 0.8; 0.9; 1.0 y 1.2 µL mL-1. El crecimiento micelial se evaluó con el tiempo, utilizando la prueba de Scott-Knott con un 5% de importancia para el análisis de resultados. De los resultados obtenidos, se descubrió que la aplicación del aceite esencial de Cymbopogon citratus interfiere significativamente con el desarrollo fúngico de la especie Aspergillus brasiliensis. La prueba de microdilución en pozos se usó cualitativamente, lo que indica la ausencia de crecimiento en dosis de 0.8 µL mL-1. Para la prueba en granos de maíz, se usaron dosis de aceite esencial de 0.5; 0.8 y 1.0 µL mL-1, habiendo sido almacenado por un período de 42 días. No hubo diferencias significativas en las diferentes dosis de aceite esencial durante el período de almacenamiento para este ensayo.O objetivo deste trabalho foi avaliar a atividade antifúngica do óleo essencial de Cymbopogon citratus no controle de Aspergillus brasiliensis em análises in vitro e em grãos de milho armazenados. Foram realizados dois testes in vitro, sendo um por difusão em ágar e o outro por microdiluição em poços. As concentrações utilizadas para o ensaio por difusão em ágar foram de 0,2; 0,4; 0,6; 0,8 e 1,0 µL mL-1. Para o teste da microdiluição em poços foram utilizadas as doses de 0,1; 0,2; 0,3; 0,4; 0,5; 0,6; 0,7; 0,8; 0,9; 1,0 e 1,2 µL mL-1. Foi avaliado o crescimento micelial ao longo do tempo, tendo sido utilizado o teste de Scott-Knott à 5% de significância para a análise dos resultados. Pelos resultados obtidos, verificou-se que a aplicação do óleo essencial de Cymbopogon citratus interfere significativamente no desenvolvimento fúngico da espécie Aspergillus brasiliensis. O teste de microdiluição em poços foi utilizado de forma qualitativa, tendo indicado ausência do crescimento em doses a partir de 0,8 µL mL-1. Para o ensaio nos grãos de milho, foram utilizadas as dosagens de óleo essencial de 0,5; 0,8 e 1,0 µL mL-1, tendo sido armazenados pelo período de 42 dias. Não houve diferença significativa das diferentes doses de óleo essencial ao longo do período de armazenamento, para este ensaio.Research, Society and Development2020-06-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/469710.33448/rsd-v9i7.4697Research, Society and Development; Vol. 9 No. 7; e691974697Research, Society and Development; Vol. 9 Núm. 7; e691974697Research, Society and Development; v. 9 n. 7; e6919746972525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/4697/4089Copyright (c) 2020 Filipe da Silva de Oliveira, Carlos Eduardo de Souza Teodoro, Pedro Amorim Berbert, Ana Paula Martinazzoinfo:eu-repo/semantics/openAccessOliveira, Filipe da Silva deTeodoro, Carlos Eduardo de SouzaBerbert, Pedro AmorimMartinazzo, Ana Paula2020-08-20T18:05:03Zoai:ojs.pkp.sfu.ca:article/4697Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:28:27.782897Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Evaluation of the antifungal potential of Cymbopogon citratus essential oil in the control of the fungus Aspergillus brasiliensis Evaluación del potencial antifúngico del aceite esencial de Cymbopogon citratus en el control del hongo Aspergillus brasiliensis Avaliação do potencial antifúngico do óleo essencial de Cymbopogon citratus no controle do fungo Aspergillus brasiliensis |
title |
Evaluation of the antifungal potential of Cymbopogon citratus essential oil in the control of the fungus Aspergillus brasiliensis |
spellingShingle |
Evaluation of the antifungal potential of Cymbopogon citratus essential oil in the control of the fungus Aspergillus brasiliensis Oliveira, Filipe da Silva de Capim-limão Grãos armazenados Fungicida natural. Lemongrass Stored grains Natural fungicide. Limoncillo Granos almacenados Fungicida natural. |
title_short |
Evaluation of the antifungal potential of Cymbopogon citratus essential oil in the control of the fungus Aspergillus brasiliensis |
title_full |
Evaluation of the antifungal potential of Cymbopogon citratus essential oil in the control of the fungus Aspergillus brasiliensis |
title_fullStr |
Evaluation of the antifungal potential of Cymbopogon citratus essential oil in the control of the fungus Aspergillus brasiliensis |
title_full_unstemmed |
Evaluation of the antifungal potential of Cymbopogon citratus essential oil in the control of the fungus Aspergillus brasiliensis |
title_sort |
Evaluation of the antifungal potential of Cymbopogon citratus essential oil in the control of the fungus Aspergillus brasiliensis |
author |
Oliveira, Filipe da Silva de |
author_facet |
Oliveira, Filipe da Silva de Teodoro, Carlos Eduardo de Souza Berbert, Pedro Amorim Martinazzo, Ana Paula |
author_role |
author |
author2 |
Teodoro, Carlos Eduardo de Souza Berbert, Pedro Amorim Martinazzo, Ana Paula |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Filipe da Silva de Teodoro, Carlos Eduardo de Souza Berbert, Pedro Amorim Martinazzo, Ana Paula |
dc.subject.por.fl_str_mv |
Capim-limão Grãos armazenados Fungicida natural. Lemongrass Stored grains Natural fungicide. Limoncillo Granos almacenados Fungicida natural. |
topic |
Capim-limão Grãos armazenados Fungicida natural. Lemongrass Stored grains Natural fungicide. Limoncillo Granos almacenados Fungicida natural. |
description |
The objective of this work was to evaluate the antifungal activity of the essential oil of Cymbopogon citratus in the control of Aspergillus brasiliensis in analysis in vitro and in stored corn kernels. Two in vitro tests were carried out. One of them, by diffusion in agar and the other by microdilution in wells. The concentrations used for the agar diffusion assay were 0.2; 0.4; 0.6; 0.8 and 1.0 µL mL-1. The concentrations used for the microdilution test were: 0.1; 0.2; 0.3; 0.4; 0.5; 0.6; 0.7; 0.8; 0.9; 1.0 and 1.2 µL mL-1. The mycelial growth was evaluated over time, using the Scott-Knott test at 5% significance. From the results obtained, it was verified that the application of the essential oil of Cymbopogon citratus interferes significantly in the fungal development of the species Aspergillus brasiliensis. The microdilution test in wells was used in a qualitative way, indicating absence of growth in doses from 0.8 µL mL-1. For the corn grain test, the essential oil dosages of 0.5; 0.8 and 1.0 µL mL-1 and were stored for 42 days. There was no significant difference in the different doses of essential oil over the storage period for this assay. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-06-04 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/4697 10.33448/rsd-v9i7.4697 |
url |
https://rsdjournal.org/index.php/rsd/article/view/4697 |
identifier_str_mv |
10.33448/rsd-v9i7.4697 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/4697/4089 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 9 No. 7; e691974697 Research, Society and Development; Vol. 9 Núm. 7; e691974697 Research, Society and Development; v. 9 n. 7; e691974697 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
_version_ |
1797052650655580160 |