Evaluation of the antifungal potential of Cymbopogon citratus essential oil in the control of the fungus Aspergillus brasiliensis

Detalhes bibliográficos
Autor(a) principal: Oliveira, Filipe da Silva de
Data de Publicação: 2020
Outros Autores: Teodoro, Carlos Eduardo de Souza, Berbert, Pedro Amorim, Martinazzo, Ana Paula
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/4697
Resumo: The objective of this work was to evaluate the antifungal activity of the essential oil of Cymbopogon citratus in the control of Aspergillus brasiliensis in analysis in vitro and in stored corn kernels. Two in vitro tests were carried out. One of them, by diffusion in agar and the other by microdilution in wells. The concentrations used for the agar diffusion assay were 0.2; 0.4; 0.6; 0.8 and 1.0 µL mL-1. The concentrations used for the microdilution test were: 0.1; 0.2; 0.3; 0.4; 0.5; 0.6; 0.7; 0.8; 0.9; 1.0 and 1.2 µL   mL-1. The mycelial growth was evaluated over time, using the Scott-Knott test at 5% significance. From the results obtained, it was verified that the application of the essential oil of Cymbopogon citratus interferes significantly in the fungal development of the species Aspergillus brasiliensis. The microdilution test in wells was used in a qualitative way, indicating absence of growth in doses from 0.8 µL mL-1. For the corn grain test, the essential oil dosages of 0.5; 0.8 and 1.0 µL mL-1 and were stored for 42 days. There was no significant difference in the different doses of essential oil over the storage period for this assay.
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spelling Evaluation of the antifungal potential of Cymbopogon citratus essential oil in the control of the fungus Aspergillus brasiliensisEvaluación del potencial antifúngico del aceite esencial de Cymbopogon citratus en el control del hongo Aspergillus brasiliensisAvaliação do potencial antifúngico do óleo essencial de Cymbopogon citratus no controle do fungo Aspergillus brasiliensisCapim-limãoGrãos armazenadosFungicida natural.LemongrassStored grainsNatural fungicide.LimoncilloGranos almacenadosFungicida natural.The objective of this work was to evaluate the antifungal activity of the essential oil of Cymbopogon citratus in the control of Aspergillus brasiliensis in analysis in vitro and in stored corn kernels. Two in vitro tests were carried out. One of them, by diffusion in agar and the other by microdilution in wells. The concentrations used for the agar diffusion assay were 0.2; 0.4; 0.6; 0.8 and 1.0 µL mL-1. The concentrations used for the microdilution test were: 0.1; 0.2; 0.3; 0.4; 0.5; 0.6; 0.7; 0.8; 0.9; 1.0 and 1.2 µL   mL-1. The mycelial growth was evaluated over time, using the Scott-Knott test at 5% significance. From the results obtained, it was verified that the application of the essential oil of Cymbopogon citratus interferes significantly in the fungal development of the species Aspergillus brasiliensis. The microdilution test in wells was used in a qualitative way, indicating absence of growth in doses from 0.8 µL mL-1. For the corn grain test, the essential oil dosages of 0.5; 0.8 and 1.0 µL mL-1 and were stored for 42 days. There was no significant difference in the different doses of essential oil over the storage period for this assay.El objetivo de este trabajo fue evaluar la actividad antifúngica del aceite esencial de Cymbopogon citratus en el control de Aspergillus brasiliensis en análisis in vitro y en granos de maíz almacenados. Se realizaron dos pruebas in vitro, una por difusión en agar y la otra por microdilución en pozos. Las concentraciones utilizadas para la prueba de difusión en agar fueron 0.2; 0.4; 0.6; 0.8 y 1.0 µL mL-1. Para la prueba de microdilución en pozos, se usaron dosis de 0.1; 0.2; 0.3; 0.4; 0,5; 0.6; 0,7; 0.8; 0.9; 1.0 y 1.2 µL mL-1. El crecimiento micelial se evaluó con el tiempo, utilizando la prueba de Scott-Knott con un 5% de importancia para el análisis de resultados. De los resultados obtenidos, se descubrió que la aplicación del aceite esencial de Cymbopogon citratus interfiere significativamente con el desarrollo fúngico de la especie Aspergillus brasiliensis. La prueba de microdilución en pozos se usó cualitativamente, lo que indica la ausencia de crecimiento en dosis de 0.8 µL mL-1. Para la prueba en granos de maíz, se usaron dosis de aceite esencial de 0.5; 0.8 y 1.0 µL mL-1, habiendo sido almacenado por un período de 42 días. No hubo diferencias significativas en las diferentes dosis de aceite esencial durante el período de almacenamiento para este ensayo.O objetivo deste trabalho foi avaliar a atividade antifúngica do óleo essencial de Cymbopogon citratus no controle de Aspergillus brasiliensis em análises in vitro e em grãos de milho armazenados. Foram realizados dois testes in vitro, sendo um por difusão em ágar e o outro por microdiluição em poços. As concentrações utilizadas para o ensaio por difusão em ágar foram de 0,2; 0,4; 0,6; 0,8 e 1,0 µL mL-1. Para o teste da microdiluição em poços foram utilizadas as doses de 0,1; 0,2; 0,3; 0,4; 0,5; 0,6; 0,7; 0,8; 0,9; 1,0 e 1,2 µL mL-1. Foi avaliado o crescimento micelial ao longo do tempo, tendo sido utilizado o teste de Scott-Knott à 5% de significância para a análise dos resultados. Pelos resultados obtidos, verificou-se que a aplicação do óleo essencial de Cymbopogon citratus interfere significativamente no desenvolvimento fúngico da espécie Aspergillus brasiliensis. O teste de microdiluição em poços foi utilizado de forma qualitativa, tendo indicado ausência do crescimento em doses a partir de 0,8 µL mL-1. Para o ensaio nos grãos de milho, foram utilizadas as dosagens de óleo essencial de 0,5; 0,8 e 1,0 µL mL-1, tendo sido armazenados pelo período de 42 dias. Não houve diferença significativa das diferentes doses de óleo essencial ao longo do período de armazenamento, para este ensaio.Research, Society and Development2020-06-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/469710.33448/rsd-v9i7.4697Research, Society and Development; Vol. 9 No. 7; e691974697Research, Society and Development; Vol. 9 Núm. 7; e691974697Research, Society and Development; v. 9 n. 7; e6919746972525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/4697/4089Copyright (c) 2020 Filipe da Silva de Oliveira, Carlos Eduardo de Souza Teodoro, Pedro Amorim Berbert, Ana Paula Martinazzoinfo:eu-repo/semantics/openAccessOliveira, Filipe da Silva deTeodoro, Carlos Eduardo de SouzaBerbert, Pedro AmorimMartinazzo, Ana Paula2020-08-20T18:05:03Zoai:ojs.pkp.sfu.ca:article/4697Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:28:27.782897Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Evaluation of the antifungal potential of Cymbopogon citratus essential oil in the control of the fungus Aspergillus brasiliensis
Evaluación del potencial antifúngico del aceite esencial de Cymbopogon citratus en el control del hongo Aspergillus brasiliensis
Avaliação do potencial antifúngico do óleo essencial de Cymbopogon citratus no controle do fungo Aspergillus brasiliensis
title Evaluation of the antifungal potential of Cymbopogon citratus essential oil in the control of the fungus Aspergillus brasiliensis
spellingShingle Evaluation of the antifungal potential of Cymbopogon citratus essential oil in the control of the fungus Aspergillus brasiliensis
Oliveira, Filipe da Silva de
Capim-limão
Grãos armazenados
Fungicida natural.
Lemongrass
Stored grains
Natural fungicide.
Limoncillo
Granos almacenados
Fungicida natural.
title_short Evaluation of the antifungal potential of Cymbopogon citratus essential oil in the control of the fungus Aspergillus brasiliensis
title_full Evaluation of the antifungal potential of Cymbopogon citratus essential oil in the control of the fungus Aspergillus brasiliensis
title_fullStr Evaluation of the antifungal potential of Cymbopogon citratus essential oil in the control of the fungus Aspergillus brasiliensis
title_full_unstemmed Evaluation of the antifungal potential of Cymbopogon citratus essential oil in the control of the fungus Aspergillus brasiliensis
title_sort Evaluation of the antifungal potential of Cymbopogon citratus essential oil in the control of the fungus Aspergillus brasiliensis
author Oliveira, Filipe da Silva de
author_facet Oliveira, Filipe da Silva de
Teodoro, Carlos Eduardo de Souza
Berbert, Pedro Amorim
Martinazzo, Ana Paula
author_role author
author2 Teodoro, Carlos Eduardo de Souza
Berbert, Pedro Amorim
Martinazzo, Ana Paula
author2_role author
author
author
dc.contributor.author.fl_str_mv Oliveira, Filipe da Silva de
Teodoro, Carlos Eduardo de Souza
Berbert, Pedro Amorim
Martinazzo, Ana Paula
dc.subject.por.fl_str_mv Capim-limão
Grãos armazenados
Fungicida natural.
Lemongrass
Stored grains
Natural fungicide.
Limoncillo
Granos almacenados
Fungicida natural.
topic Capim-limão
Grãos armazenados
Fungicida natural.
Lemongrass
Stored grains
Natural fungicide.
Limoncillo
Granos almacenados
Fungicida natural.
description The objective of this work was to evaluate the antifungal activity of the essential oil of Cymbopogon citratus in the control of Aspergillus brasiliensis in analysis in vitro and in stored corn kernels. Two in vitro tests were carried out. One of them, by diffusion in agar and the other by microdilution in wells. The concentrations used for the agar diffusion assay were 0.2; 0.4; 0.6; 0.8 and 1.0 µL mL-1. The concentrations used for the microdilution test were: 0.1; 0.2; 0.3; 0.4; 0.5; 0.6; 0.7; 0.8; 0.9; 1.0 and 1.2 µL   mL-1. The mycelial growth was evaluated over time, using the Scott-Knott test at 5% significance. From the results obtained, it was verified that the application of the essential oil of Cymbopogon citratus interferes significantly in the fungal development of the species Aspergillus brasiliensis. The microdilution test in wells was used in a qualitative way, indicating absence of growth in doses from 0.8 µL mL-1. For the corn grain test, the essential oil dosages of 0.5; 0.8 and 1.0 µL mL-1 and were stored for 42 days. There was no significant difference in the different doses of essential oil over the storage period for this assay.
publishDate 2020
dc.date.none.fl_str_mv 2020-06-04
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/4697
10.33448/rsd-v9i7.4697
url https://rsdjournal.org/index.php/rsd/article/view/4697
identifier_str_mv 10.33448/rsd-v9i7.4697
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/4697/4089
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 9 No. 7; e691974697
Research, Society and Development; Vol. 9 Núm. 7; e691974697
Research, Society and Development; v. 9 n. 7; e691974697
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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