Colorimetric methods using synthetic and alternative reagents for pollen viability of Bixa orellana L.
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/5817 |
Resumo: | The study of pollen viability is essential for genetic improvement, as it shows the fertility of species. Thus, estimates of pollen grains of B. orellana L., in viable and non-viable, are necessary in order to search for efficient, safe and preferably, accessible and easy-to-manipulate dyes to distinguish the reproductive potential of male gametes in the annatto. The objective was to evaluate the potential of five colorimetric tests, three synthetic and two alternative reagents, in order to estimate the pollen viability of B. orellana L. A mix of 50 flower buds in the pre-anthesis stage was collected in different locations, in the municipality of Alta Floresta - MT. In the laboratory, the pollen grains were tested with three synthetic dyes: Alexander reagent, 2% Lugol and Roeser method (Astra blue + Fuchsin); Two more natural, extracted from the red poppy flower (Hibisco sp.) And from the Jatobá bark (Hymenaea courbaril L.). Pollen viability was estimated by the percentage of viable and non-viable pollen grains. A completely randomized design was used, consisting of 5 treatments and 10 repetitions. The data were submitted to the Tukey test at 5% probability. The synthetic dyes Reagent de alexander and Lugol 2% obtained the highest averages, however, they did not differ by the average test of the alternative dye of Hibisco sp. which proved to be suitable and recommended for use in cytogenetics due to its low cost, ease of acquisition and handling, making it a strategic alternative when compared to conventional synthetic dyes. |
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Colorimetric methods using synthetic and alternative reagents for pollen viability of Bixa orellana L.Métodos colorimétricos que utilizan reactivos sintéticos y alternativos para la viabilidad del polen de Bixa orellana L.Métodos colorimétricos sob uso de reagentes sintéticos e alternativos para viabilidade polínica de Bixa orellana L.Azul-de-astraFucsinaLugolReagente de alexanderUrucum.Ostra azulFucsinaLugolAlexander reactivoAnnatto.Oyster blueFuchsinLugolAlexander reactiveAnnatto.The study of pollen viability is essential for genetic improvement, as it shows the fertility of species. Thus, estimates of pollen grains of B. orellana L., in viable and non-viable, are necessary in order to search for efficient, safe and preferably, accessible and easy-to-manipulate dyes to distinguish the reproductive potential of male gametes in the annatto. The objective was to evaluate the potential of five colorimetric tests, three synthetic and two alternative reagents, in order to estimate the pollen viability of B. orellana L. A mix of 50 flower buds in the pre-anthesis stage was collected in different locations, in the municipality of Alta Floresta - MT. In the laboratory, the pollen grains were tested with three synthetic dyes: Alexander reagent, 2% Lugol and Roeser method (Astra blue + Fuchsin); Two more natural, extracted from the red poppy flower (Hibisco sp.) And from the Jatobá bark (Hymenaea courbaril L.). Pollen viability was estimated by the percentage of viable and non-viable pollen grains. A completely randomized design was used, consisting of 5 treatments and 10 repetitions. The data were submitted to the Tukey test at 5% probability. The synthetic dyes Reagent de alexander and Lugol 2% obtained the highest averages, however, they did not differ by the average test of the alternative dye of Hibisco sp. which proved to be suitable and recommended for use in cytogenetics due to its low cost, ease of acquisition and handling, making it a strategic alternative when compared to conventional synthetic dyes.El estudio de la viabilidad del polen es esencial para la mejora genética, ya que muestra la fertilidad de las especies. Por lo tanto, las estimaciones de los granos de polen de B. orellana L., en viables y no viables, son necesarios para buscar tintes eficientes, seguros y preferiblemente accesibles y fáciles de manejar para distinguir el potencial reproductivo de los gametos masculinos en el annatto El objetivo fue evaluar el potencial de cinco pruebas colorimétricas, tres reactivos sintéticos y dos reactivos alternativos, para estimar la viabilidad del polen de B. orellana L. Se recolectó una mezcla de 50 botones florales en la etapa previa a la antesis en diferentes lugares, en el municipio de Alta Floresta - MT. En el laboratorio, los granos de polen se analizaron con tres colorantes sintéticos: reactivo Alexander, 2% de método Lugol y Roeser (azul Astra + fucsina); Dos más naturales, extraídos de la flor de amapola roja (Hibisco sp.) Y de la corteza de Jatobá (Hymenaea courbaril L.). La viabilidad del polen se estimó por el porcentaje de granos de polen viables y no viables. Se utilizó un diseño completamente al azar, que consta de 5 tratamientos y 10 repeticiones. Los datos se sometieron a la prueba de Tukey con una probabilidad del 5%. Los colorantes sintéticos Reactivo de alexander y Lugol 2% obtuvieron los promedios más altos, sin embargo, no difirieron en la prueba promedio del colorante alternativo de Hibisco sp. que demostró ser adecuado y recomendado para su uso en citogenética debido a su bajo costo, facilidad de adquisición y manejo, lo que lo convierte en una alternativa estratégica en comparación con los colorantes sintéticos convencionales.O estudo de viabilidade polínica é essencial para o melhoramento genético, pois evidencia a fertilidade das espécies. Assim, testes estimativos de grãos de pólens de B. orellana L., em viáveis e inviáveis, mostram-se necessários a fim de se buscar corantes eficientes, seguros e preferencialmente, acessíveis e de fácil manipulação para distinção do potencial reprodutivo dos gametas masculinos do urucum. Objetivou-se avaliar a potencialidade de cinco testes colorimétricos, três reagentes sintéticos e dois alternativos, com o intuito de estimar a viabilidade polínica da B. orellana L. Um mix de 50 botões florais em estádio de pré-antese foi coletado em diferentes localidades, no município de Alta Floresta - MT. Em laboratório, os grãos de pólens foram testados com três corantes sintéticos: Reagente de alexander, Lugol 2% e método de Roeser (Azul-de-astra + Fucsina); Mais dois naturais, extraídos da flor de papoula vermelha (Hibisco sp.) e da casca de Jatobá (Hymenaea courbaril L.). A viabilidade polínica foi estimada pela percentagem de grãos de pólen viáveis e inviáveis. Foi utilizado o delineamento inteiramente casualizado, composto por 5 tratamentos e 10 repetições. Os dados foram submetidos ao teste de Tukey a 5% de probabilidade. Os corantes sintéticos Reagente de alexander e Lugol 2% obtiveram as maiores médias, no entanto, não diferiram pelo teste de média do corante alternativo de Hibisco sp. que demonstrou ser adequado e recomendável ao uso na citogenética pelo baixo custo, facilidade de aquisição e manuseio, tornando-se uma alternativa estratégica quando comparado aos corantes sintéticos convencionais.Research, Society and Development2020-07-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/581710.33448/rsd-v9i8.5817Research, Society and Development; Vol. 9 No. 8; e330985817Research, Society and Development; Vol. 9 Núm. 8; e330985817Research, Society and Development; v. 9 n. 8; e3309858172525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/5817/4863Copyright (c) 2020 Thatielen Furini, Maicon de Souza Pecegueiro, Fernando Herrmann, Jean Carlos Silva, Joelson de Oliveira Barros, Samiele Camargo de Oliveira Domingues, Isane Vera Karsburginfo:eu-repo/semantics/openAccessFurini, ThatielenPecegueiro, Maicon de SouzaHerrmann, FernandoSilva, Jean CarlosBarros, Joelson de OliveiraDomingues, Samiele Camargo de OliveiraKarsburg, Isane Vera2020-08-20T18:00:17Zoai:ojs.pkp.sfu.ca:article/5817Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:29:12.980530Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Colorimetric methods using synthetic and alternative reagents for pollen viability of Bixa orellana L. Métodos colorimétricos que utilizan reactivos sintéticos y alternativos para la viabilidad del polen de Bixa orellana L. Métodos colorimétricos sob uso de reagentes sintéticos e alternativos para viabilidade polínica de Bixa orellana L. |
title |
Colorimetric methods using synthetic and alternative reagents for pollen viability of Bixa orellana L. |
spellingShingle |
Colorimetric methods using synthetic and alternative reagents for pollen viability of Bixa orellana L. Furini, Thatielen Azul-de-astra Fucsina Lugol Reagente de alexander Urucum. Ostra azul Fucsina Lugol Alexander reactivo Annatto. Oyster blue Fuchsin Lugol Alexander reactive Annatto. |
title_short |
Colorimetric methods using synthetic and alternative reagents for pollen viability of Bixa orellana L. |
title_full |
Colorimetric methods using synthetic and alternative reagents for pollen viability of Bixa orellana L. |
title_fullStr |
Colorimetric methods using synthetic and alternative reagents for pollen viability of Bixa orellana L. |
title_full_unstemmed |
Colorimetric methods using synthetic and alternative reagents for pollen viability of Bixa orellana L. |
title_sort |
Colorimetric methods using synthetic and alternative reagents for pollen viability of Bixa orellana L. |
author |
Furini, Thatielen |
author_facet |
Furini, Thatielen Pecegueiro, Maicon de Souza Herrmann, Fernando Silva, Jean Carlos Barros, Joelson de Oliveira Domingues, Samiele Camargo de Oliveira Karsburg, Isane Vera |
author_role |
author |
author2 |
Pecegueiro, Maicon de Souza Herrmann, Fernando Silva, Jean Carlos Barros, Joelson de Oliveira Domingues, Samiele Camargo de Oliveira Karsburg, Isane Vera |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Furini, Thatielen Pecegueiro, Maicon de Souza Herrmann, Fernando Silva, Jean Carlos Barros, Joelson de Oliveira Domingues, Samiele Camargo de Oliveira Karsburg, Isane Vera |
dc.subject.por.fl_str_mv |
Azul-de-astra Fucsina Lugol Reagente de alexander Urucum. Ostra azul Fucsina Lugol Alexander reactivo Annatto. Oyster blue Fuchsin Lugol Alexander reactive Annatto. |
topic |
Azul-de-astra Fucsina Lugol Reagente de alexander Urucum. Ostra azul Fucsina Lugol Alexander reactivo Annatto. Oyster blue Fuchsin Lugol Alexander reactive Annatto. |
description |
The study of pollen viability is essential for genetic improvement, as it shows the fertility of species. Thus, estimates of pollen grains of B. orellana L., in viable and non-viable, are necessary in order to search for efficient, safe and preferably, accessible and easy-to-manipulate dyes to distinguish the reproductive potential of male gametes in the annatto. The objective was to evaluate the potential of five colorimetric tests, three synthetic and two alternative reagents, in order to estimate the pollen viability of B. orellana L. A mix of 50 flower buds in the pre-anthesis stage was collected in different locations, in the municipality of Alta Floresta - MT. In the laboratory, the pollen grains were tested with three synthetic dyes: Alexander reagent, 2% Lugol and Roeser method (Astra blue + Fuchsin); Two more natural, extracted from the red poppy flower (Hibisco sp.) And from the Jatobá bark (Hymenaea courbaril L.). Pollen viability was estimated by the percentage of viable and non-viable pollen grains. A completely randomized design was used, consisting of 5 treatments and 10 repetitions. The data were submitted to the Tukey test at 5% probability. The synthetic dyes Reagent de alexander and Lugol 2% obtained the highest averages, however, they did not differ by the average test of the alternative dye of Hibisco sp. which proved to be suitable and recommended for use in cytogenetics due to its low cost, ease of acquisition and handling, making it a strategic alternative when compared to conventional synthetic dyes. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-07-06 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/5817 10.33448/rsd-v9i8.5817 |
url |
https://rsdjournal.org/index.php/rsd/article/view/5817 |
identifier_str_mv |
10.33448/rsd-v9i8.5817 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/5817/4863 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 9 No. 8; e330985817 Research, Society and Development; Vol. 9 Núm. 8; e330985817 Research, Society and Development; v. 9 n. 8; e330985817 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052737740865536 |