Neuroprotective effects of taurine on SH-SY5Y cells under hydrocortisone induced stress

Detalhes bibliográficos
Autor(a) principal: Rossato, Rafaella Carvalho
Data de Publicação: 2021
Outros Autores: Granato, Alessandro Eustaquio Campos, Moraes, Carlos Dailton Guedes de Oliveira, Salles, Geisa Nogueira, Soares, Cristina Pacheco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/18426
Resumo: Alzheimer's disease (AD) is the most common, progressive and irreversible neurodegenerative disorder, characterized by memory loss, cognitive impairment and behavioral abnormalities. Although there is no cure, several study strategies seek to elucidate mechanisms of the disease. Recent studies address the benefits of taurine. Thus, the present study aims to analyze neuroprotective effects of taurine in human neuroblastoma (SH-SY5Y), using an in vitro experimental model of oxidative stress induced by hydrocortisone. This work showed for the first time that taurine can promote neuroprotection in SH-SY5Y under oxidative stress caused by hydrocortisone. Cell viability was evaluated using crystal violet and the evaluation of cell morphology was performed by scanning electron microscopy (SEM). The viability of SH-SY5Y pre-treated with taurine and stressed with hydrocortisone was preserved, compared to the stressed only group, which was also morphologically observed. Therefore, taurine can represent a considerable therapeutic candidate in the prevention of neurodegenerative diseases, such as AD.
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spelling Neuroprotective effects of taurine on SH-SY5Y cells under hydrocortisone induced stressEfectos neuroprotectivos de la taurina en células SH-SY5Y bajo estrés por hidrocortisonaEfeitos neuroprotetores da taurina em células SH-SY5Y sob estresse induzido por hidrocortisonaDoença de AlzheimerEstresse OxidativoNeuroproteçãoHidrocortisona.Enfermedad de AlzheimerEstrés oxidativoNeuroprotecciónHidrocortisona.Alzheimer's DiseaseOxidative StressNeuroprotectionHydrocortisone.Alzheimer's disease (AD) is the most common, progressive and irreversible neurodegenerative disorder, characterized by memory loss, cognitive impairment and behavioral abnormalities. Although there is no cure, several study strategies seek to elucidate mechanisms of the disease. Recent studies address the benefits of taurine. Thus, the present study aims to analyze neuroprotective effects of taurine in human neuroblastoma (SH-SY5Y), using an in vitro experimental model of oxidative stress induced by hydrocortisone. This work showed for the first time that taurine can promote neuroprotection in SH-SY5Y under oxidative stress caused by hydrocortisone. Cell viability was evaluated using crystal violet and the evaluation of cell morphology was performed by scanning electron microscopy (SEM). The viability of SH-SY5Y pre-treated with taurine and stressed with hydrocortisone was preserved, compared to the stressed only group, which was also morphologically observed. Therefore, taurine can represent a considerable therapeutic candidate in the prevention of neurodegenerative diseases, such as AD.La enfermedad de Alzheimer (EA) es el trastorno neurodegenerativo más común, progresivo e irreversible, caracterizado por pérdida de memoria, deterioro cognitivo y alteraciones del comportamiento. Aunque no existe cura, varias estrategias de estudio buscan dilucidar los mecanismos de la enfermedad. Estudios recientes abordan los beneficios de la taurina. Así, el presente estudio tiene como objetivo analizar el efecto neuroprotector de la taurina en el neuroblastoma humano (SH-SY5Y), utilizando un modelo experimental in vitro de estrés oxidativo inducido por hidrocortisona. Este trabajo demostró por primera vez que la taurina puede promover la neuroprotección en las células SH-SY5Y bajo el estrés oxidativo causado por la hidrocortisona. La viabilidad celular se evaluó utilizando cristal violeta y la evaluación de la morfología celular se realizó mediante microscopía electrónica de barrido (MEB). Se conservó la viabilidad de las células SH-SY5Y pretratadas con taurina y estresadas con hidrocortisona, en comparación con el grupo expuesto solo a hidrocortisona, que también se observó morfológicamente. Por tanto, la taurina puede representar un importante candidato terapéutico en la prevención de enfermedades neurodegenerativas como la EA.A doença de Alzheimer (DA) é o distúrbio neurodegenerativo mais comum, progressivo e irreversível, caracterizado por perda de memória, prejuízo cognitivo e anormalidades comportamentais. Embora não haja cura, várias estratégias de estudo buscam elucidar os mecanismos da doença. Estudos recentes abordam os benefícios da taurina. Assim, o presente estudo tem como objetivo analisar o efeito neuroprotetor da taurina em neuroblastoma humano (SH-SY5Y), utilizando um modelo experimental in vitro de estresse oxidativo induzido por hidrocortisona. Este trabalho mostrou pela primeira vez que a taurina pode promover a neuroproteção nas células SH-SY5Y sob o estresse oxidativo causado pela hidrocortisona. A viabilidade celular foi avaliada utilizando cristal violeta e a avaliação da morfologia celular foi realizada por microscopia eletrônica de varredura (MEV). A viabilidade das células SH-SY5Y pré-tratadas com taurina e estressadas com hidrocortisona foi preservada, em comparação com o grupo exposto apenas à hidrocortisona, o que foi também observado morfologicamente. Portanto, a taurina pode representar um importante candidato terapêutico na prevenção de doenças neurodegenerativas, como a DA.Research, Society and Development2021-08-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1842610.33448/rsd-v10i9.18426Research, Society and Development; Vol. 10 No. 9; e55510918426Research, Society and Development; Vol. 10 Núm. 9; e55510918426Research, Society and Development; v. 10 n. 9; e555109184262525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/18426/16516Copyright (c) 2021 Rafaella Carvalho Rossato; Alessandro Eustaquio Campos Granato; Carlos Dailton Guedes de Oliveira Moraes; Geisa Nogueira Salles; Cristina Pacheco Soareshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessRossato, Rafaella Carvalho Granato, Alessandro Eustaquio Campos Moraes, Carlos Dailton Guedes de Oliveira Salles, Geisa Nogueira Soares, Cristina Pacheco 2021-09-12T14:28:06Zoai:ojs.pkp.sfu.ca:article/18426Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:38:34.262765Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Neuroprotective effects of taurine on SH-SY5Y cells under hydrocortisone induced stress
Efectos neuroprotectivos de la taurina en células SH-SY5Y bajo estrés por hidrocortisona
Efeitos neuroprotetores da taurina em células SH-SY5Y sob estresse induzido por hidrocortisona
title Neuroprotective effects of taurine on SH-SY5Y cells under hydrocortisone induced stress
spellingShingle Neuroprotective effects of taurine on SH-SY5Y cells under hydrocortisone induced stress
Rossato, Rafaella Carvalho
Doença de Alzheimer
Estresse Oxidativo
Neuroproteção
Hidrocortisona.
Enfermedad de Alzheimer
Estrés oxidativo
Neuroprotección
Hidrocortisona.
Alzheimer's Disease
Oxidative Stress
Neuroprotection
Hydrocortisone.
title_short Neuroprotective effects of taurine on SH-SY5Y cells under hydrocortisone induced stress
title_full Neuroprotective effects of taurine on SH-SY5Y cells under hydrocortisone induced stress
title_fullStr Neuroprotective effects of taurine on SH-SY5Y cells under hydrocortisone induced stress
title_full_unstemmed Neuroprotective effects of taurine on SH-SY5Y cells under hydrocortisone induced stress
title_sort Neuroprotective effects of taurine on SH-SY5Y cells under hydrocortisone induced stress
author Rossato, Rafaella Carvalho
author_facet Rossato, Rafaella Carvalho
Granato, Alessandro Eustaquio Campos
Moraes, Carlos Dailton Guedes de Oliveira
Salles, Geisa Nogueira
Soares, Cristina Pacheco
author_role author
author2 Granato, Alessandro Eustaquio Campos
Moraes, Carlos Dailton Guedes de Oliveira
Salles, Geisa Nogueira
Soares, Cristina Pacheco
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Rossato, Rafaella Carvalho
Granato, Alessandro Eustaquio Campos
Moraes, Carlos Dailton Guedes de Oliveira
Salles, Geisa Nogueira
Soares, Cristina Pacheco
dc.subject.por.fl_str_mv Doença de Alzheimer
Estresse Oxidativo
Neuroproteção
Hidrocortisona.
Enfermedad de Alzheimer
Estrés oxidativo
Neuroprotección
Hidrocortisona.
Alzheimer's Disease
Oxidative Stress
Neuroprotection
Hydrocortisone.
topic Doença de Alzheimer
Estresse Oxidativo
Neuroproteção
Hidrocortisona.
Enfermedad de Alzheimer
Estrés oxidativo
Neuroprotección
Hidrocortisona.
Alzheimer's Disease
Oxidative Stress
Neuroprotection
Hydrocortisone.
description Alzheimer's disease (AD) is the most common, progressive and irreversible neurodegenerative disorder, characterized by memory loss, cognitive impairment and behavioral abnormalities. Although there is no cure, several study strategies seek to elucidate mechanisms of the disease. Recent studies address the benefits of taurine. Thus, the present study aims to analyze neuroprotective effects of taurine in human neuroblastoma (SH-SY5Y), using an in vitro experimental model of oxidative stress induced by hydrocortisone. This work showed for the first time that taurine can promote neuroprotection in SH-SY5Y under oxidative stress caused by hydrocortisone. Cell viability was evaluated using crystal violet and the evaluation of cell morphology was performed by scanning electron microscopy (SEM). The viability of SH-SY5Y pre-treated with taurine and stressed with hydrocortisone was preserved, compared to the stressed only group, which was also morphologically observed. Therefore, taurine can represent a considerable therapeutic candidate in the prevention of neurodegenerative diseases, such as AD.
publishDate 2021
dc.date.none.fl_str_mv 2021-08-03
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/18426
10.33448/rsd-v10i9.18426
url https://rsdjournal.org/index.php/rsd/article/view/18426
identifier_str_mv 10.33448/rsd-v10i9.18426
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/18426/16516
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 9; e55510918426
Research, Society and Development; Vol. 10 Núm. 9; e55510918426
Research, Society and Development; v. 10 n. 9; e55510918426
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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