Evaluation of genetic diversity of Cryptococcus neoformans samples isolated in different geographical regions

Detalhes bibliográficos
Autor(a) principal: Araújo, Dakson Douglas
Data de Publicação: 2021
Outros Autores: Silva, Raí Emanuel da, Pereira, Higinalice da Silva, Costa, Dacylla Sampaio, Sá, Rodrigo Elísio de, Silva Neto, Antonio Rodrigues da, Alves, Nathanael dos Santos, Fonseca, Fernanda Machado
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/18333
Resumo: Cryptococcus neoformans is the etiological agent of cryptococcosis, an opportunistic fungal infection and frequently occurs in immunocompromised patients, with an underlying disease, mainly affecting the central nervous system and lungs. Analysis of genomic sequences of microorganisms through phylogenies has demonstrated a wide spectrum of applications in scientific research and medicine. In the health area, the use of this tool contributes to the diagnosis and consequently to the development of programs to improve the treatment and control of diseases. Furthermore, the phylogenetic analysis is important to characterize new species that can development possible genetic recombinations, which in most cases is responsible for the resistance of pathogens to the treatment. Thus, this study aimed to analyze the genetic variability of 18S and 28S genes of C. neoformans in different geographic regions. Were selected 191 sequences of the 18S gene and 132 sequences of the 28S gene to carry out phylogenetic and split analyses. The results demonstrated that the 28S gene has more genetic variability when compared to the 18S gene, which has been shown to be more conserved. This variability can be a result from genetic recombination and migration of different strains from one geographic region to another through the tourism of individuals with the fungal. Additionally, in the future, this genetic recombination can result in a new species capable of causing diseases in humans.
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spelling Evaluation of genetic diversity of Cryptococcus neoformans samples isolated in different geographical regionsEvaluación de la diversidad genética de muestras de Cryptococcus neoformans aisladas en diferentes regiones geográficasAvaliação da diversidade genética de amostras de Cryptococcus neoformans isoladas em diferentes regiões geográficasCryptococcus neoformansPhylogenyRecombination genetic.Cryptococcus neoformansFilogeniaRecombinación genética.Cryptococcus neoformansFilogeniaRecombinação genética.Cryptococcus neoformans is the etiological agent of cryptococcosis, an opportunistic fungal infection and frequently occurs in immunocompromised patients, with an underlying disease, mainly affecting the central nervous system and lungs. Analysis of genomic sequences of microorganisms through phylogenies has demonstrated a wide spectrum of applications in scientific research and medicine. In the health area, the use of this tool contributes to the diagnosis and consequently to the development of programs to improve the treatment and control of diseases. Furthermore, the phylogenetic analysis is important to characterize new species that can development possible genetic recombinations, which in most cases is responsible for the resistance of pathogens to the treatment. Thus, this study aimed to analyze the genetic variability of 18S and 28S genes of C. neoformans in different geographic regions. Were selected 191 sequences of the 18S gene and 132 sequences of the 28S gene to carry out phylogenetic and split analyses. The results demonstrated that the 28S gene has more genetic variability when compared to the 18S gene, which has been shown to be more conserved. This variability can be a result from genetic recombination and migration of different strains from one geographic region to another through the tourism of individuals with the fungal. Additionally, in the future, this genetic recombination can result in a new species capable of causing diseases in humans.Cryptococcus neoformans es el agente etiológico de la criptococosis, una infección micótica oportunista y ocurre con frecuencia en pacientes inmunodeprimidos, con una enfermedad subyacente, que afecta principalmente al sistema nervioso central y los pulmones. El análisis de secuencias genómicas de microorganismos mediante filogenias ha mostrado un amplio espectro de aplicaciones en la investigación científica y la medicina. En el área de la salud, el uso de esta herramienta ayuda en el diagnóstico y, en consecuencia, en la elaboración de programas para un mejor tratamiento y control de enfermedades. Además, el análisis filogenético es importante para caracterizar nuevas especies que puedan sufrir una posible recombinación genética, que en la mayoría de los casos es responsable de la resistencia de los patógenos al tratamiento. Así, este estudio tuvo como objetivo analizar la variabilidad genética de los genes 18S y 28S de C. neoformans en diferentes regiones geográficas. Se seleccionaron 191 secuencias de genes del gen 18S y 132 secuencias de genes del gen 28S para llevar a cabo los análisis filogenéticos y Split. Los resultados mostraron que el gen 28S tiene una mayor variabilidad genética en comparación con el gen 18S, que resultó estar más conservado. Esta variabilidad puede resultar de recombinaciones genéticas y migraciones de diferentes cepas de una región geográfica a otra, por ejemplo, a través del turismo de individuos portadores del hongo. Además, la recombinación genética puede resultar en el futuro en una nueva especie capaz de causar enfermedades en el hombre.Cryptococcus neoformans é o agente etiológico da criptococose, uma infecção fúngica oportunista e ocorre frequentemente em pacientes imunodeprimidos, com uma doença de base, afetando principalmente o sistema nervoso central e os pulmões. Análises de sequências genômicas de microrganismos por meio de filogenias têm demonstrado um amplo espectro de aplicações na pesquisa científica e na medicina. Na área da saúde, a utilização desta ferramenta auxilia no diagnóstico e consequentemente na elaboração de programas para melhor tratamento e controle das doenças. Ainda, a análise filogenética é importante para caracterizar novas espécies que possam vir a sofrer possíveis recombinações genéticas, que na grande maioria das vezes é responsável pela resistência de patógenos frente ao tratamento. Desta forma, o presente estudo teve como objetivo analisar a variabilidade genética dos genes 18S e 28S de C. neoformans em diferentes regiões geográficas. Foram selecionadas 191 sequências gênicas do gene 18S e 132 sequências gênicas do gene 28S para realização das análises filogenéticas e Split. Os resultados demonstraram que o gene 28S tem uma maior variabilidade genética se comparado ao gene 18S que se mostrou mais conservado. Essa variabilidade pode ser resultante de recombinações genéticas e migrações de cepas diferentes de uma região geográfica para outra, como por exemplo, através do turismo de indivíduos portadores do fungo. Além disso, a recombinação genética pode resultar futuramente em uma nova espécie com capacidade de causar doenças no homem.Research, Society and Development2021-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1833310.33448/rsd-v10i9.18333Research, Society and Development; Vol. 10 No. 9; e51410918333Research, Society and Development; Vol. 10 Núm. 9; e51410918333Research, Society and Development; v. 10 n. 9; e514109183332525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/18333/16425Copyright (c) 2021 Dakson Douglas Araújo; Raí Emanuel da Silva; Higinalice da Silva Pereira; Dacylla Sampaio Costa; Rodrigo Elísio de Sá; Antonio Rodrigues da Silva Neto; Nathanael dos Santos Alves; Fernanda Machado Fonsecahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessAraújo, Dakson DouglasSilva, Raí Emanuel daPereira, Higinalice da Silva Costa, Dacylla Sampaio Sá, Rodrigo Elísio de Silva Neto, Antonio Rodrigues da Alves, Nathanael dos Santos Fonseca, Fernanda Machado2021-09-12T14:28:06Zoai:ojs.pkp.sfu.ca:article/18333Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:38:29.751895Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Evaluation of genetic diversity of Cryptococcus neoformans samples isolated in different geographical regions
Evaluación de la diversidad genética de muestras de Cryptococcus neoformans aisladas en diferentes regiones geográficas
Avaliação da diversidade genética de amostras de Cryptococcus neoformans isoladas em diferentes regiões geográficas
title Evaluation of genetic diversity of Cryptococcus neoformans samples isolated in different geographical regions
spellingShingle Evaluation of genetic diversity of Cryptococcus neoformans samples isolated in different geographical regions
Araújo, Dakson Douglas
Cryptococcus neoformans
Phylogeny
Recombination genetic.
Cryptococcus neoformans
Filogenia
Recombinación genética.
Cryptococcus neoformans
Filogenia
Recombinação genética.
title_short Evaluation of genetic diversity of Cryptococcus neoformans samples isolated in different geographical regions
title_full Evaluation of genetic diversity of Cryptococcus neoformans samples isolated in different geographical regions
title_fullStr Evaluation of genetic diversity of Cryptococcus neoformans samples isolated in different geographical regions
title_full_unstemmed Evaluation of genetic diversity of Cryptococcus neoformans samples isolated in different geographical regions
title_sort Evaluation of genetic diversity of Cryptococcus neoformans samples isolated in different geographical regions
author Araújo, Dakson Douglas
author_facet Araújo, Dakson Douglas
Silva, Raí Emanuel da
Pereira, Higinalice da Silva
Costa, Dacylla Sampaio
Sá, Rodrigo Elísio de
Silva Neto, Antonio Rodrigues da
Alves, Nathanael dos Santos
Fonseca, Fernanda Machado
author_role author
author2 Silva, Raí Emanuel da
Pereira, Higinalice da Silva
Costa, Dacylla Sampaio
Sá, Rodrigo Elísio de
Silva Neto, Antonio Rodrigues da
Alves, Nathanael dos Santos
Fonseca, Fernanda Machado
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Araújo, Dakson Douglas
Silva, Raí Emanuel da
Pereira, Higinalice da Silva
Costa, Dacylla Sampaio
Sá, Rodrigo Elísio de
Silva Neto, Antonio Rodrigues da
Alves, Nathanael dos Santos
Fonseca, Fernanda Machado
dc.subject.por.fl_str_mv Cryptococcus neoformans
Phylogeny
Recombination genetic.
Cryptococcus neoformans
Filogenia
Recombinación genética.
Cryptococcus neoformans
Filogenia
Recombinação genética.
topic Cryptococcus neoformans
Phylogeny
Recombination genetic.
Cryptococcus neoformans
Filogenia
Recombinación genética.
Cryptococcus neoformans
Filogenia
Recombinação genética.
description Cryptococcus neoformans is the etiological agent of cryptococcosis, an opportunistic fungal infection and frequently occurs in immunocompromised patients, with an underlying disease, mainly affecting the central nervous system and lungs. Analysis of genomic sequences of microorganisms through phylogenies has demonstrated a wide spectrum of applications in scientific research and medicine. In the health area, the use of this tool contributes to the diagnosis and consequently to the development of programs to improve the treatment and control of diseases. Furthermore, the phylogenetic analysis is important to characterize new species that can development possible genetic recombinations, which in most cases is responsible for the resistance of pathogens to the treatment. Thus, this study aimed to analyze the genetic variability of 18S and 28S genes of C. neoformans in different geographic regions. Were selected 191 sequences of the 18S gene and 132 sequences of the 28S gene to carry out phylogenetic and split analyses. The results demonstrated that the 28S gene has more genetic variability when compared to the 18S gene, which has been shown to be more conserved. This variability can be a result from genetic recombination and migration of different strains from one geographic region to another through the tourism of individuals with the fungal. Additionally, in the future, this genetic recombination can result in a new species capable of causing diseases in humans.
publishDate 2021
dc.date.none.fl_str_mv 2021-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/18333
10.33448/rsd-v10i9.18333
url https://rsdjournal.org/index.php/rsd/article/view/18333
identifier_str_mv 10.33448/rsd-v10i9.18333
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/18333/16425
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 9; e51410918333
Research, Society and Development; Vol. 10 Núm. 9; e51410918333
Research, Society and Development; v. 10 n. 9; e51410918333
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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