The environmental yeast Cryptococcus liquefaciens produces capsular and secreted polysaccharides with similar pathogenic properties to those of C. neoformans
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | http://dx.doi.org/10.1038/srep46768 http://hdl.handle.net/1843/56306 https://orcid.org/0000-0002-5478-9006 https://orcid.org/0000-0001-6852-6912 https://orcid.org/0000-0002-1783-1228 https://orcid.org/0000-0002-1895-1299 https://orcid.org/0000-0002-1108-5666 https://orcid.org/0000-0001-5875-9886 |
Resumo: | Invasive fungal infections, including cryptococcosis, are a growing threat to immunocompromised patients. Although Cryptococcus neoformans and Cryptococcus gattii are the main agents of human cryptococcosis, opportunistic infections by environmental species, such as C. liquefaciens, have been observed recently. The main Cryptococcus virulence factor is the production and secretion of polysaccharides (PS). Previously, we showed that both species produce PS of similar composition. Here, we examined the ultrastructure and biological activity of capsular and secreted PS from C. liquefaciens, and yeast pathogenicity to an invertebrate host, in comparison with C. neoformans. Ultrastructural analysis by high-resolution microscopy showed that both species produce large and complex capsules. PS from both species had indistinguishable effects on phagocytosis levels, NO production and the secretion of a variety of immune mediators. Challenge with C. liquefaciens or C. neoformans led to complete lethality of G. mellonella larvae. Treatment with C. liquefaciens PS could not protect mice against infection with C. neoformans. We conclude that polysaccharides of the environmental yeast C. liquefaciens have strikingly similar ultrastructural and biological properties to those of C. neoformans, highlighting the importance of monitoring the emergence of new fungal pathogens for which thermotolerance may be an important transitional step towards pathogenesis in humans. |
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2023-07-14T20:38:47Z2023-07-14T20:38:47Z20177http://dx.doi.org/10.1038/srep467682045-2322http://hdl.handle.net/1843/56306https://orcid.org/0000-0002-5478-9006https://orcid.org/0000-0001-6852-6912https://orcid.org/0000-0002-1783-1228https://orcid.org/0000-0002-1895-1299https://orcid.org/0000-0002-1108-5666https://orcid.org/0000-0001-5875-9886Invasive fungal infections, including cryptococcosis, are a growing threat to immunocompromised patients. Although Cryptococcus neoformans and Cryptococcus gattii are the main agents of human cryptococcosis, opportunistic infections by environmental species, such as C. liquefaciens, have been observed recently. The main Cryptococcus virulence factor is the production and secretion of polysaccharides (PS). Previously, we showed that both species produce PS of similar composition. Here, we examined the ultrastructure and biological activity of capsular and secreted PS from C. liquefaciens, and yeast pathogenicity to an invertebrate host, in comparison with C. neoformans. Ultrastructural analysis by high-resolution microscopy showed that both species produce large and complex capsules. PS from both species had indistinguishable effects on phagocytosis levels, NO production and the secretion of a variety of immune mediators. Challenge with C. liquefaciens or C. neoformans led to complete lethality of G. mellonella larvae. Treatment with C. liquefaciens PS could not protect mice against infection with C. neoformans. We conclude that polysaccharides of the environmental yeast C. liquefaciens have strikingly similar ultrastructural and biological properties to those of C. neoformans, highlighting the importance of monitoring the emergence of new fungal pathogens for which thermotolerance may be an important transitional step towards pathogenesis in humans.Infecções fúngicas invasivas, incluindo criptococose, são uma ameaça crescente para pacientes imunocomprometidos. Embora Cryptococcus neoformans e Cryptococcus gattii sejam os principais agentes da criptococose humana, infecções oportunistas por espécies ambientais, como C. liquefaciens, têm sido observadas recentemente. O principal fator de virulência do Cryptococcus é a produção e secreção de polissacarídeos (PS). Anteriormente, mostramos que ambas as espécies produzem PS de composição semelhante. Aqui, examinamos a ultraestrutura e a atividade biológica do PS capsular e secretado de C. liquefaciens e a patogenicidade da levedura para um hospedeiro invertebrado, em comparação com C. neoformans. A análise ultraestrutural por microscopia de alta resolução mostrou que ambas as espécies produzem cápsulas grandes e complexas. PS de ambas as espécies tiveram efeitos indistinguíveis nos níveis de fagocitose, produção de NO e secreção de uma variedade de mediadores imunológicos. O desafio com C. liquefaciens ou C. neoformans levou à letalidade completa das larvas de G. mellonella. O tratamento com C. liquefaciens PS não protegeu os camundongos contra a infecção por C. neoformans. Concluímos que os polissacarídeos da levedura ambiental C. liquefaciens têm propriedades ultraestruturais e biológicas surpreendentemente semelhantes às de C. neoformans, destacando a importância de monitorar o surgimento de novos patógenos fúngicos para os quais a termotolerância pode ser um importante passo de transição para a patogênese em humanos.engUniversidade Federal de Minas GeraisUFMGBrasilICB - DEPARTAMENTO DE MICROBIOLOGIAScientific reportsCryptococcusPolissacarídeosCryptococcus neoformansCriptococoseCryptococcus liquefaciensPolysaccharidesCryptococcus neoformansThe environmental yeast Cryptococcus liquefaciens produces capsular and secreted polysaccharides with similar pathogenic properties to those of C. neoformansA levedura ambiental Cryptococcus liquefaciens produz polissacarídeos capsulares e secretados com propriedades patogênicas semelhantes às de C. neoformansinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.nature.com/articles/srep46768Glauber Ribeiro de Sousa AraújoGustavo J. C. FreitasFernanda Lopes FonsecaPaulo Emilio LeiteGustavo Miranda RochaWanderley de SouzaDaniel de Assis SantosSusana Frasesapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/56306/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALThe environmental yeast Cryptococcus liquefaciens produces capsular and secreted polysaccharides with similar pathogenic properties to those of C. neoformans.pdfThe environmental yeast Cryptococcus liquefaciens produces capsular and secreted polysaccharides with similar pathogenic properties to those of C. neoformans.pdfapplication/pdf21986798https://repositorio.ufmg.br/bitstream/1843/56306/2/The%20environmental%20yeast%20Cryptococcus%20liquefaciens%20produces%20capsular%20and%20secreted%20polysaccharides%20with%20similar%20pathogenic%20properties%20to%20those%20of%20C.%20neoformans.pdf0de8f4e9d90b3257e3582c1d616ec30bMD521843/563062023-07-14 17:38:47.88oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-07-14T20:38:47Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
The environmental yeast Cryptococcus liquefaciens produces capsular and secreted polysaccharides with similar pathogenic properties to those of C. neoformans |
dc.title.alternative.pt_BR.fl_str_mv |
A levedura ambiental Cryptococcus liquefaciens produz polissacarídeos capsulares e secretados com propriedades patogênicas semelhantes às de C. neoformans |
title |
The environmental yeast Cryptococcus liquefaciens produces capsular and secreted polysaccharides with similar pathogenic properties to those of C. neoformans |
spellingShingle |
The environmental yeast Cryptococcus liquefaciens produces capsular and secreted polysaccharides with similar pathogenic properties to those of C. neoformans Glauber Ribeiro de Sousa Araújo Cryptococcus liquefaciens Polysaccharides Cryptococcus neoformans Cryptococcus Polissacarídeos Cryptococcus neoformans Criptococose |
title_short |
The environmental yeast Cryptococcus liquefaciens produces capsular and secreted polysaccharides with similar pathogenic properties to those of C. neoformans |
title_full |
The environmental yeast Cryptococcus liquefaciens produces capsular and secreted polysaccharides with similar pathogenic properties to those of C. neoformans |
title_fullStr |
The environmental yeast Cryptococcus liquefaciens produces capsular and secreted polysaccharides with similar pathogenic properties to those of C. neoformans |
title_full_unstemmed |
The environmental yeast Cryptococcus liquefaciens produces capsular and secreted polysaccharides with similar pathogenic properties to those of C. neoformans |
title_sort |
The environmental yeast Cryptococcus liquefaciens produces capsular and secreted polysaccharides with similar pathogenic properties to those of C. neoformans |
author |
Glauber Ribeiro de Sousa Araújo |
author_facet |
Glauber Ribeiro de Sousa Araújo Gustavo J. C. Freitas Fernanda Lopes Fonseca Paulo Emilio Leite Gustavo Miranda Rocha Wanderley de Souza Daniel de Assis Santos Susana Frases |
author_role |
author |
author2 |
Gustavo J. C. Freitas Fernanda Lopes Fonseca Paulo Emilio Leite Gustavo Miranda Rocha Wanderley de Souza Daniel de Assis Santos Susana Frases |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Glauber Ribeiro de Sousa Araújo Gustavo J. C. Freitas Fernanda Lopes Fonseca Paulo Emilio Leite Gustavo Miranda Rocha Wanderley de Souza Daniel de Assis Santos Susana Frases |
dc.subject.por.fl_str_mv |
Cryptococcus liquefaciens Polysaccharides Cryptococcus neoformans |
topic |
Cryptococcus liquefaciens Polysaccharides Cryptococcus neoformans Cryptococcus Polissacarídeos Cryptococcus neoformans Criptococose |
dc.subject.other.pt_BR.fl_str_mv |
Cryptococcus Polissacarídeos Cryptococcus neoformans Criptococose |
description |
Invasive fungal infections, including cryptococcosis, are a growing threat to immunocompromised patients. Although Cryptococcus neoformans and Cryptococcus gattii are the main agents of human cryptococcosis, opportunistic infections by environmental species, such as C. liquefaciens, have been observed recently. The main Cryptococcus virulence factor is the production and secretion of polysaccharides (PS). Previously, we showed that both species produce PS of similar composition. Here, we examined the ultrastructure and biological activity of capsular and secreted PS from C. liquefaciens, and yeast pathogenicity to an invertebrate host, in comparison with C. neoformans. Ultrastructural analysis by high-resolution microscopy showed that both species produce large and complex capsules. PS from both species had indistinguishable effects on phagocytosis levels, NO production and the secretion of a variety of immune mediators. Challenge with C. liquefaciens or C. neoformans led to complete lethality of G. mellonella larvae. Treatment with C. liquefaciens PS could not protect mice against infection with C. neoformans. We conclude that polysaccharides of the environmental yeast C. liquefaciens have strikingly similar ultrastructural and biological properties to those of C. neoformans, highlighting the importance of monitoring the emergence of new fungal pathogens for which thermotolerance may be an important transitional step towards pathogenesis in humans. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2023-07-14T20:38:47Z |
dc.date.available.fl_str_mv |
2023-07-14T20:38:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/56306 |
dc.identifier.doi.pt_BR.fl_str_mv |
http://dx.doi.org/10.1038/srep46768 |
dc.identifier.issn.pt_BR.fl_str_mv |
2045-2322 |
dc.identifier.orcid.pt_BR.fl_str_mv |
https://orcid.org/0000-0002-5478-9006 https://orcid.org/0000-0001-6852-6912 https://orcid.org/0000-0002-1783-1228 https://orcid.org/0000-0002-1895-1299 https://orcid.org/0000-0002-1108-5666 https://orcid.org/0000-0001-5875-9886 |
url |
http://dx.doi.org/10.1038/srep46768 http://hdl.handle.net/1843/56306 https://orcid.org/0000-0002-5478-9006 https://orcid.org/0000-0001-6852-6912 https://orcid.org/0000-0002-1783-1228 https://orcid.org/0000-0002-1895-1299 https://orcid.org/0000-0002-1108-5666 https://orcid.org/0000-0001-5875-9886 |
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2045-2322 |
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eng |
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eng |
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Scientific reports |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade Federal de Minas Gerais |
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UFMG |
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Brasil |
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ICB - DEPARTAMENTO DE MICROBIOLOGIA |
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Universidade Federal de Minas Gerais |
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