Efficacy of Caryocar brasiliense Camb. and Annona crassiflora Mart. leaves in the reduction of biotransferred Escherichia coli from Lactuca sativa L. leaves to polypropylene

Detalhes bibliográficos
Autor(a) principal: Borges, Larissa Lorrane Rodrigues
Data de Publicação: 2021
Outros Autores: Guimarães, Alécia Daila Barros, Morais-Costa, Fanciellen, Duarte, Eduardo Robson, Martins, Márcia, Careli, Roberta Torres
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/24268
Resumo: The antimicrobial activity of Caryocar brasiliense and Annona crassiflora leaves extracts was evaluated against Escherichia coli strains through a sensitivity test by the agar diffusion method and by the evaluation of minimal inhibitory concentrations (MIC) and minimal bactericidal concentrations (MBC) by the microdilution technique followed by plating. The sanitizing efficiency of these extracts in reducing E. coli cells biotransferred from L. sativa leaves to polypropylene surfaces was evaluated after an exposure time of 5 minutes. All strains were sensitive to antibacterials and the formation of an inhibition halo was observed for the extracts against all strains tested. The C. brasiliense extract showed better antimicrobial action against E. coli strains, with a MIC of 1.09 mg/mL, while the MIC of A. crassiflora extract was 5.58 mg/mL. No MBC was found for plant extracts. The strains were not able to form biofilms under the conditions studied, however, there was biotransfer and adhesion of E. coli to polypropylene. The highest counts of E. coli cells biotransferred and, consequently, adhere to the polypropylene coupons were observed when 5 log CFU/mL was inoculated, with a mean count of 4.53 ± 0.66 log CFU/cm2. It was verified that the treatment with the extract solutions in the minimum inhibitory concentrations (MIC) totally reduced the number of E. coli cells adhered to the polypropylene coupons. The results obtained indicate that the use of extracts from both species as antibacterials is promising.
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spelling Efficacy of Caryocar brasiliense Camb. and Annona crassiflora Mart. leaves in the reduction of biotransferred Escherichia coli from Lactuca sativa L. leaves to polypropyleneEficacia de hojas de Caryocar brasiliense Camb. y Annona crassiflora Mart. en la reducción de Escherichia coli biotransferida de hojas de Lactuca sativa L. a polipropilenoEficácia de extratos de folhas de Caryocar brasiliense Camb. e Annona crassiflora Mart. na redução de Escherichia coli biotransferida de folhas de Lactuca sativa L. para polipropilenoBacterial adhesionBiofilmsCross contaminationSanitization.Adhesión bacterianaBiopelículasContaminación cruzadaHigienización.Adesão bacterianaBiofilmesContaminação cruzadaSanitização.The antimicrobial activity of Caryocar brasiliense and Annona crassiflora leaves extracts was evaluated against Escherichia coli strains through a sensitivity test by the agar diffusion method and by the evaluation of minimal inhibitory concentrations (MIC) and minimal bactericidal concentrations (MBC) by the microdilution technique followed by plating. The sanitizing efficiency of these extracts in reducing E. coli cells biotransferred from L. sativa leaves to polypropylene surfaces was evaluated after an exposure time of 5 minutes. All strains were sensitive to antibacterials and the formation of an inhibition halo was observed for the extracts against all strains tested. The C. brasiliense extract showed better antimicrobial action against E. coli strains, with a MIC of 1.09 mg/mL, while the MIC of A. crassiflora extract was 5.58 mg/mL. No MBC was found for plant extracts. The strains were not able to form biofilms under the conditions studied, however, there was biotransfer and adhesion of E. coli to polypropylene. The highest counts of E. coli cells biotransferred and, consequently, adhere to the polypropylene coupons were observed when 5 log CFU/mL was inoculated, with a mean count of 4.53 ± 0.66 log CFU/cm2. It was verified that the treatment with the extract solutions in the minimum inhibitory concentrations (MIC) totally reduced the number of E. coli cells adhered to the polypropylene coupons. The results obtained indicate that the use of extracts from both species as antibacterials is promising.La actividad antimicrobiana de los extractos de hojas de Caryocar brasiliense y Annona crassiflora se evaluó frente a cepas de Escherichia coli mediante una prueba de sensibilidad por el método de difusión en agar y mediante la evaluación de concentraciones mínimas inhibitorias (CMI) y concentraciones mínimas bactericidas (CMB) mediante la técnica de microdilución seguida de enchapado. La eficacia higienizante de estos extractos para reducir la biotransferencia de células de E. coli de hojas de L. sativa a superficies de polipropileno se evaluó después de un tiempo de exposición de 5 minutos. Todas las cepas fueron sensibles a los antibacterianos y se observó la formación de un halo de inhibición para los extractos en estudio frente a todas las cepas probadas. El extracto de C. brasiliense mostró una mejor acción antimicrobiana contra las cepas de E. coli, con una CMI de 1.09 mg/mL, mientras que la CMI del extracto de A. crassiflora fue de 5.58 mg/mL. No se encontró CMB para extractos de plantas. Las cepas no pudieron formar biopelículas en las condiciones estudiadas, sin embargo, hubo biotransferencia y adhesión de E. coli al polipropileno. Los recuentos más altos de células de E. coli biotransferidas y, en consecuencia, adheridas a los cupones de polipropileno se observaron cuando se inocularon 5 log UFC/mL con un recuento medio de 4,53 ± 0,66 log UFC/cm2. Se verificó que el tratamiento con las soluciones de extracto en las concentraciones mínimas inhibitorias (CMI), redujo totalmente el número de células de E. coli adheridas a los cupones de polipropileno. Los resultados obtenidos indican que el uso de extractos de ambas especies como antibacterianos es prometedor.A atividade antimicrobiana de extratos de folhas de Caryocar brasiliense e Annona crassiflora foi avaliada frente cepas de Escherichia coli através de teste de sensibilidade pelo método de difusão em ágar e pela avaliação das concentrações inibitórias mínimas (CIM) e as concentrações bactericidas mínimas (CBM) pela técnica de microdiluição seguida de plaqueamento. A eficiência sanitizante desses extratos na redução de células de E. coli biotransferidas de folhas de Lactuca sativa para superfícies de polipropileno, foi avaliada após o tempo de exposição de 5 minutos. Todas as cepas foram sensíveis aos antibacterianos e observou-se a formação de halo de inibição para os extratos em estudo frente a todas as cepas testadas. O extrato de C. brasiliense apresentou melhor ação antimicrobiana frente às cepas de E. coli, com CIM de 1,09 mg/mL, enquanto que a CIM do extrato de A. crassiflora foi de 5,58 mg/mL. Não foi encontrada CBM para os extratos vegetais. As estirpes não foram capazes de formar biofilmes nas condições estudadas, no entanto, houve biotransferência e adesão de E. coli ao polipropileno. As maiores contagens de células de E. coli biotransferidas e, consequentemente, aderidas aos cupons de polipropileno foram observadas quando se inoculou 5 log UFC/mL com contagem média de 4,53 ± 0,66 log UFC/cm2. Verificou-se que o tratamento com as soluções dos extratos nas concentrações inibitórias mínimas (CIM), reduziu totalmente o número de células de E. coli aderidas nos cupons de polipropileno. Os resultados obtidos indicam que a utilização dos extratos de ambas as espécies como antibacterianos é promissora.Research, Society and Development2021-12-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2426810.33448/rsd-v10i17.24268Research, Society and Development; Vol. 10 No. 17; e124101724268Research, Society and Development; Vol. 10 Núm. 17; e124101724268Research, Society and Development; v. 10 n. 17; e1241017242682525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/24268/21416Copyright (c) 2021 Larissa Lorrane Rodrigues Borges; Alécia Daila Barros Guimarães; Fanciellen Morais-Costa; Eduardo Robson Duarte; Márcia Martins; Roberta Torres Carelihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessBorges, Larissa Lorrane Rodrigues Guimarães, Alécia Daila Barros Morais-Costa, FanciellenDuarte, Eduardo RobsonMartins, Márcia Careli, Roberta Torres 2022-01-01T11:11:08Zoai:ojs.pkp.sfu.ca:article/24268Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:42:53.135467Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Efficacy of Caryocar brasiliense Camb. and Annona crassiflora Mart. leaves in the reduction of biotransferred Escherichia coli from Lactuca sativa L. leaves to polypropylene
Eficacia de hojas de Caryocar brasiliense Camb. y Annona crassiflora Mart. en la reducción de Escherichia coli biotransferida de hojas de Lactuca sativa L. a polipropileno
Eficácia de extratos de folhas de Caryocar brasiliense Camb. e Annona crassiflora Mart. na redução de Escherichia coli biotransferida de folhas de Lactuca sativa L. para polipropileno
title Efficacy of Caryocar brasiliense Camb. and Annona crassiflora Mart. leaves in the reduction of biotransferred Escherichia coli from Lactuca sativa L. leaves to polypropylene
spellingShingle Efficacy of Caryocar brasiliense Camb. and Annona crassiflora Mart. leaves in the reduction of biotransferred Escherichia coli from Lactuca sativa L. leaves to polypropylene
Borges, Larissa Lorrane Rodrigues
Bacterial adhesion
Biofilms
Cross contamination
Sanitization.
Adhesión bacteriana
Biopelículas
Contaminación cruzada
Higienización.
Adesão bacteriana
Biofilmes
Contaminação cruzada
Sanitização.
title_short Efficacy of Caryocar brasiliense Camb. and Annona crassiflora Mart. leaves in the reduction of biotransferred Escherichia coli from Lactuca sativa L. leaves to polypropylene
title_full Efficacy of Caryocar brasiliense Camb. and Annona crassiflora Mart. leaves in the reduction of biotransferred Escherichia coli from Lactuca sativa L. leaves to polypropylene
title_fullStr Efficacy of Caryocar brasiliense Camb. and Annona crassiflora Mart. leaves in the reduction of biotransferred Escherichia coli from Lactuca sativa L. leaves to polypropylene
title_full_unstemmed Efficacy of Caryocar brasiliense Camb. and Annona crassiflora Mart. leaves in the reduction of biotransferred Escherichia coli from Lactuca sativa L. leaves to polypropylene
title_sort Efficacy of Caryocar brasiliense Camb. and Annona crassiflora Mart. leaves in the reduction of biotransferred Escherichia coli from Lactuca sativa L. leaves to polypropylene
author Borges, Larissa Lorrane Rodrigues
author_facet Borges, Larissa Lorrane Rodrigues
Guimarães, Alécia Daila Barros
Morais-Costa, Fanciellen
Duarte, Eduardo Robson
Martins, Márcia
Careli, Roberta Torres
author_role author
author2 Guimarães, Alécia Daila Barros
Morais-Costa, Fanciellen
Duarte, Eduardo Robson
Martins, Márcia
Careli, Roberta Torres
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Borges, Larissa Lorrane Rodrigues
Guimarães, Alécia Daila Barros
Morais-Costa, Fanciellen
Duarte, Eduardo Robson
Martins, Márcia
Careli, Roberta Torres
dc.subject.por.fl_str_mv Bacterial adhesion
Biofilms
Cross contamination
Sanitization.
Adhesión bacteriana
Biopelículas
Contaminación cruzada
Higienización.
Adesão bacteriana
Biofilmes
Contaminação cruzada
Sanitização.
topic Bacterial adhesion
Biofilms
Cross contamination
Sanitization.
Adhesión bacteriana
Biopelículas
Contaminación cruzada
Higienización.
Adesão bacteriana
Biofilmes
Contaminação cruzada
Sanitização.
description The antimicrobial activity of Caryocar brasiliense and Annona crassiflora leaves extracts was evaluated against Escherichia coli strains through a sensitivity test by the agar diffusion method and by the evaluation of minimal inhibitory concentrations (MIC) and minimal bactericidal concentrations (MBC) by the microdilution technique followed by plating. The sanitizing efficiency of these extracts in reducing E. coli cells biotransferred from L. sativa leaves to polypropylene surfaces was evaluated after an exposure time of 5 minutes. All strains were sensitive to antibacterials and the formation of an inhibition halo was observed for the extracts against all strains tested. The C. brasiliense extract showed better antimicrobial action against E. coli strains, with a MIC of 1.09 mg/mL, while the MIC of A. crassiflora extract was 5.58 mg/mL. No MBC was found for plant extracts. The strains were not able to form biofilms under the conditions studied, however, there was biotransfer and adhesion of E. coli to polypropylene. The highest counts of E. coli cells biotransferred and, consequently, adhere to the polypropylene coupons were observed when 5 log CFU/mL was inoculated, with a mean count of 4.53 ± 0.66 log CFU/cm2. It was verified that the treatment with the extract solutions in the minimum inhibitory concentrations (MIC) totally reduced the number of E. coli cells adhered to the polypropylene coupons. The results obtained indicate that the use of extracts from both species as antibacterials is promising.
publishDate 2021
dc.date.none.fl_str_mv 2021-12-22
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/24268
10.33448/rsd-v10i17.24268
url https://rsdjournal.org/index.php/rsd/article/view/24268
identifier_str_mv 10.33448/rsd-v10i17.24268
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/24268/21416
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 17; e124101724268
Research, Society and Development; Vol. 10 Núm. 17; e124101724268
Research, Society and Development; v. 10 n. 17; e124101724268
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052810300227584

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