Analysis of biomolecular changes caused by exposure to the drug diclofenac sodium in fish of the Brycon Opalinus species using Fourier transformed infrared spectroscopy (FTIR)

Detalhes bibliográficos
Autor(a) principal: Banhara, Vanessa Fernandez
Data de Publicação: 2022
Outros Autores: Mello, Andriele Maisa de Oliveira, Lyra, Lucas Ferreira, Silva, Maria Regina de Aquino, Sakane, Kumiko Koibuchi
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/36080
Resumo: The increase in the consumption of medicines around the world has generated several studies on the impacts caused by the inappropriate disposal of waste containing the active ingredients of medicines in the environment. After being ingested, these drugs are excreted in the form of other compounds in domestic sewage, many of which are still persistent in the environment and generate physiological changes in aquatic organisms. Due to population growth and excessive consumption, the anti-inflammatory known as diclofenac sodium is among the main drugs found in Brazilian surface waters, in concentrations in the range of micrograms per liter (µg.L-1). This work aimed to study the biomolecular changes that diclofenac can cause in fish of the Brycon opalinus species, using the Fourier Transform Infrared Spectroscopy (FTIR) technique coupled with chemometric methods, principal component analysis (PCA) and hierarchical cluster analysis (HCA). Two different concentrations of diclofenac sodium plus the control were studied. The organ selected for this study were the gills. PCA and HCA results showed discrimination between the control and treated spectra. The quantitative analysis, by calculating the areas of the bands of lipids, proteins and carbohydrates, showed changes in these biomolecules, with proteins and carbohydrates being more sensitive to exposure to diclofenac. The results of the deconvolution and curve fitting showed a decrease in the disordered structure and an increase in β sheet of the protein secondary structure for the exposure of 4.4 µg.L-1 of diclofenac.
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spelling Analysis of biomolecular changes caused by exposure to the drug diclofenac sodium in fish of the Brycon Opalinus species using Fourier transformed infrared spectroscopy (FTIR)Análisis de los cambios en las biomoléculas causados por la exposición al fármaco diclofenaco sódico en peces de la especie Brycon opalinus mediante espectroscopia infrarroja por transformada de Fourier (FTIR)Análise das alterações de biomoléculas causadas pela exposição ao fármaco diclofenaco sódico em peixes da espécie Brycon Opalinus utilizando a espectroscopia no infravermelho por transformada de Fourier (FTIR)Espectroscopia Vibracional no Infravermelho MédioBrycon opalinusEcotoxicidadeFármacosMeio ambiente.Espectroscopia vibracional en el infrarrojo medioBrycon opalinusEcotoxicidadFármacosMedioambiente.Infrared SpectroscopyBrycon opalinusEcotoxicityDrugsEnvironment.The increase in the consumption of medicines around the world has generated several studies on the impacts caused by the inappropriate disposal of waste containing the active ingredients of medicines in the environment. After being ingested, these drugs are excreted in the form of other compounds in domestic sewage, many of which are still persistent in the environment and generate physiological changes in aquatic organisms. Due to population growth and excessive consumption, the anti-inflammatory known as diclofenac sodium is among the main drugs found in Brazilian surface waters, in concentrations in the range of micrograms per liter (µg.L-1). This work aimed to study the biomolecular changes that diclofenac can cause in fish of the Brycon opalinus species, using the Fourier Transform Infrared Spectroscopy (FTIR) technique coupled with chemometric methods, principal component analysis (PCA) and hierarchical cluster analysis (HCA). Two different concentrations of diclofenac sodium plus the control were studied. The organ selected for this study were the gills. PCA and HCA results showed discrimination between the control and treated spectra. The quantitative analysis, by calculating the areas of the bands of lipids, proteins and carbohydrates, showed changes in these biomolecules, with proteins and carbohydrates being more sensitive to exposure to diclofenac. The results of the deconvolution and curve fitting showed a decrease in the disordered structure and an increase in β sheet of the protein secondary structure for the exposure of 4.4 µg.L-1 of diclofenac.El aumento del consumo de medicamentos en el mundo generó varios estudios sobre los impactos causados por la eliminación inadecuada de residuos que contienen los principios activos de los medicamentos en el medioambiente. Tras ingerirlos, estos fármacos son excretados como otros compuestos en las aguas residuales domésticas, varios aún persisten en el ambiente y generan cambios fisiológicos en los organismos acuáticos. Debido al crecimiento poblacional y su consumo excesivo, el antiinflamatorio diclofenaco sódico está entre los principales fármacos encontrados en las aguas superficiales brasileñas en concentraciones en el orden de microgramos por litro (µg.L-¹). Este trabajo tuvo como objetivo estudiar las alteraciones biomoleculares que este medicamento puede generar en los peces de la especie Brycon opalinus, utilizando la técnica de espectroscopia infrarroja por transformada de Fourier (FTIR) acoplada a métodos quimiométricos, análisis de componentes principales (PCA) y análisis de clúster jerárquico (HCA). Se analizaron dos concentraciones diferentes del fármaco más el control. El órgano seleccionado para el análisis fueron las branquias. Los resultados del PCA y HCA mostraron la distinción entre los espectros de control y los tratados. El análisis cuantitativo mediante el cálculo de las áreas de las bandas de lípidos, proteínas y carbohidratos mostró cambios en estas biomoléculas, siendo las proteínas y los carbohidratos más sensibles a exposición al diclofenaco. Los resultados de la deconvolución y ajuste de la curva mostraron disminución de la estructura desordenada y aumento de la hoja β de la estructura secundaria de la proteína para la exposición de 4,4 µg.L-¹ de diclofenaco.O aumento no consumo de medicamentos em todo mundo tem gerado vários estudos sobre os impactos causados pelo descarte inadequado de resíduos que contenham os princípios ativos dos medicamentos no meio ambiente. Após serem ingeridos, estes medicamentos são excretados na forma de outros compostos no esgoto doméstico, que vários ainda são persistentes no ambiente e geram alterações fisiológicas em organismos aquáticos. Devido ao crescimento populacional e o consumo excessivo, o anti-inflamatório conhecido como diclofenaco sódico, está entre os principais medicamentos encontrados nas águas superficiais brasileiras, em concentrações na faixa de micrograma por litro (µg.L-1). Este trabalho teve como objetivo estudar as alterações biomoleculares que o diclofenaco pode gerar em peixes da espécie Brycon opalinus, utilizando a técnica de Espectroscopia no infravermelho por transformada de Fourier (FTIR) acoplada aos métodos quimiométricos, a análise de componentes principais (PCA) e a análise de agrupamento hierárquico (HCA). Foram estudadas duas diferentes concentrações de diclofenaco mais o controle. O órgão selecionado para ser estudado foram as brânquias. Os resultados de PCA e HCA mostraram a discriminação entre os espectros de controle e tratados. A análise quantitativa através do cálculo das áreas das bandas de lipídios, proteínas e carboidratos mostrou alterações nestas biomoléculas, sendo que proteínas e carboidratos são mais sensíveis a exposição ao diclofenaco. Os resultados de deconvolução e ajuste de curvas mostraram a diminuição de estrutura desordenada e aumento de β folha da estrutura secundária de proteína para a exposição de 4,4 µg.L-1 de diclofenaco.Research, Society and Development2022-10-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/3608010.33448/rsd-v11i14.36080Research, Society and Development; Vol. 11 No. 14; e201111436080Research, Society and Development; Vol. 11 Núm. 14; e201111436080Research, Society and Development; v. 11 n. 14; e2011114360802525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/36080/30275Copyright (c) 2022 Vanessa Fernandez Banhara; Andriele Maisa de Oliveira Mello; Lucas Ferreira Lyra; Maria Regina de Aquino Silva; Kumiko Koibuchi Sakanehttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessBanhara, Vanessa Fernandez Mello, Andriele Maisa de OliveiraLyra, Lucas Ferreira Silva, Maria Regina de Aquino Sakane, Kumiko Koibuchi 2022-11-08T13:36:27Zoai:ojs.pkp.sfu.ca:article/36080Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:50:45.781410Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Analysis of biomolecular changes caused by exposure to the drug diclofenac sodium in fish of the Brycon Opalinus species using Fourier transformed infrared spectroscopy (FTIR)
Análisis de los cambios en las biomoléculas causados por la exposición al fármaco diclofenaco sódico en peces de la especie Brycon opalinus mediante espectroscopia infrarroja por transformada de Fourier (FTIR)
Análise das alterações de biomoléculas causadas pela exposição ao fármaco diclofenaco sódico em peixes da espécie Brycon Opalinus utilizando a espectroscopia no infravermelho por transformada de Fourier (FTIR)
title Analysis of biomolecular changes caused by exposure to the drug diclofenac sodium in fish of the Brycon Opalinus species using Fourier transformed infrared spectroscopy (FTIR)
spellingShingle Analysis of biomolecular changes caused by exposure to the drug diclofenac sodium in fish of the Brycon Opalinus species using Fourier transformed infrared spectroscopy (FTIR)
Banhara, Vanessa Fernandez
Espectroscopia Vibracional no Infravermelho Médio
Brycon opalinus
Ecotoxicidade
Fármacos
Meio ambiente.
Espectroscopia vibracional en el infrarrojo medio
Brycon opalinus
Ecotoxicidad
Fármacos
Medioambiente.
Infrared Spectroscopy
Brycon opalinus
Ecotoxicity
Drugs
Environment.
title_short Analysis of biomolecular changes caused by exposure to the drug diclofenac sodium in fish of the Brycon Opalinus species using Fourier transformed infrared spectroscopy (FTIR)
title_full Analysis of biomolecular changes caused by exposure to the drug diclofenac sodium in fish of the Brycon Opalinus species using Fourier transformed infrared spectroscopy (FTIR)
title_fullStr Analysis of biomolecular changes caused by exposure to the drug diclofenac sodium in fish of the Brycon Opalinus species using Fourier transformed infrared spectroscopy (FTIR)
title_full_unstemmed Analysis of biomolecular changes caused by exposure to the drug diclofenac sodium in fish of the Brycon Opalinus species using Fourier transformed infrared spectroscopy (FTIR)
title_sort Analysis of biomolecular changes caused by exposure to the drug diclofenac sodium in fish of the Brycon Opalinus species using Fourier transformed infrared spectroscopy (FTIR)
author Banhara, Vanessa Fernandez
author_facet Banhara, Vanessa Fernandez
Mello, Andriele Maisa de Oliveira
Lyra, Lucas Ferreira
Silva, Maria Regina de Aquino
Sakane, Kumiko Koibuchi
author_role author
author2 Mello, Andriele Maisa de Oliveira
Lyra, Lucas Ferreira
Silva, Maria Regina de Aquino
Sakane, Kumiko Koibuchi
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Banhara, Vanessa Fernandez
Mello, Andriele Maisa de Oliveira
Lyra, Lucas Ferreira
Silva, Maria Regina de Aquino
Sakane, Kumiko Koibuchi
dc.subject.por.fl_str_mv Espectroscopia Vibracional no Infravermelho Médio
Brycon opalinus
Ecotoxicidade
Fármacos
Meio ambiente.
Espectroscopia vibracional en el infrarrojo medio
Brycon opalinus
Ecotoxicidad
Fármacos
Medioambiente.
Infrared Spectroscopy
Brycon opalinus
Ecotoxicity
Drugs
Environment.
topic Espectroscopia Vibracional no Infravermelho Médio
Brycon opalinus
Ecotoxicidade
Fármacos
Meio ambiente.
Espectroscopia vibracional en el infrarrojo medio
Brycon opalinus
Ecotoxicidad
Fármacos
Medioambiente.
Infrared Spectroscopy
Brycon opalinus
Ecotoxicity
Drugs
Environment.
description The increase in the consumption of medicines around the world has generated several studies on the impacts caused by the inappropriate disposal of waste containing the active ingredients of medicines in the environment. After being ingested, these drugs are excreted in the form of other compounds in domestic sewage, many of which are still persistent in the environment and generate physiological changes in aquatic organisms. Due to population growth and excessive consumption, the anti-inflammatory known as diclofenac sodium is among the main drugs found in Brazilian surface waters, in concentrations in the range of micrograms per liter (µg.L-1). This work aimed to study the biomolecular changes that diclofenac can cause in fish of the Brycon opalinus species, using the Fourier Transform Infrared Spectroscopy (FTIR) technique coupled with chemometric methods, principal component analysis (PCA) and hierarchical cluster analysis (HCA). Two different concentrations of diclofenac sodium plus the control were studied. The organ selected for this study were the gills. PCA and HCA results showed discrimination between the control and treated spectra. The quantitative analysis, by calculating the areas of the bands of lipids, proteins and carbohydrates, showed changes in these biomolecules, with proteins and carbohydrates being more sensitive to exposure to diclofenac. The results of the deconvolution and curve fitting showed a decrease in the disordered structure and an increase in β sheet of the protein secondary structure for the exposure of 4.4 µg.L-1 of diclofenac.
publishDate 2022
dc.date.none.fl_str_mv 2022-10-24
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/36080
10.33448/rsd-v11i14.36080
url https://rsdjournal.org/index.php/rsd/article/view/36080
identifier_str_mv 10.33448/rsd-v11i14.36080
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/36080/30275
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 11 No. 14; e201111436080
Research, Society and Development; Vol. 11 Núm. 14; e201111436080
Research, Society and Development; v. 11 n. 14; e201111436080
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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