Vegetative propagation by mini-cuttings of Brazil nut “Bertholletia excelsa Bonpl” with the aid of rhizobacteria mixes
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/41947 |
Resumo: | The aim of this work was to analyze the vegetative propagation of Bertholletia excelsa through the mini-cutting technique, with the aid of rhizobacteria mixes. The methodology used was based on the collection of roots of B. excelsa, from which rhizobacteria were bioprospected. From them we formed mixes, for inoculation of mini-cuttings in the plantings in tubes containing commercial substrate, after 90 days they were dug up to collect the data. The experiment was conducted in a completely randomized design, consisting of 6 treatments (5 mixes and controls) with 6 replications, with 1 mini-cutting for each replication. The data were made available in an excel table. The variables analyzed were: survival, callus formation, formation and length of mini-cutting roots. According to MINITAB, the survival of mini-cuttings treated with rhizobacteria x controls presented a significant difference by the KRUSKAL-WALLIS test (p-0.01), for the other variables there was no significant variation. The MAO5PB mix, consisting of MAOPB12A, 12K, 12L, 12J and 12C bacillus, through qualitative analysis, was selected as the most productive and the 5 bacteria that constitute it were molecularly identified: PB12A as Bacillus wiedmannii; 12K as B. paramycoides; 12L as Lysinibacillus fusiformis; 12J as B. paramycoides and 12C as Lysinibacillus fusiformis. This work allows to affirm that it is possible to propagate B. Excelsa through the minicutting technique helped by rhizobacteria and contribute to reduce the pressure in the forest, with recovery of degraded areas, increase commercial production and support small producers in the B. Excelsa wood and nut production chain. |
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Vegetative propagation by mini-cuttings of Brazil nut “Bertholletia excelsa Bonpl” with the aid of rhizobacteria mixes Propagación vegetativa por miniesquejes de nuez de Brasil “Bertholletia excelsa Bonpl” con la ayuda de mezclas de rizobacterias Propagação vegetativa por miniestacas de Castanha-do-Brasil “Bertholletia excelsa Bonpl” com auxílio de mixes de rizobactérias ProspecçãoInoculaçãoCastanha-do-Brasil.ProspecciónInoculaciónNuez de Brasil.ProspectingInoculationBrazil nut.The aim of this work was to analyze the vegetative propagation of Bertholletia excelsa through the mini-cutting technique, with the aid of rhizobacteria mixes. The methodology used was based on the collection of roots of B. excelsa, from which rhizobacteria were bioprospected. From them we formed mixes, for inoculation of mini-cuttings in the plantings in tubes containing commercial substrate, after 90 days they were dug up to collect the data. The experiment was conducted in a completely randomized design, consisting of 6 treatments (5 mixes and controls) with 6 replications, with 1 mini-cutting for each replication. The data were made available in an excel table. The variables analyzed were: survival, callus formation, formation and length of mini-cutting roots. According to MINITAB, the survival of mini-cuttings treated with rhizobacteria x controls presented a significant difference by the KRUSKAL-WALLIS test (p-0.01), for the other variables there was no significant variation. The MAO5PB mix, consisting of MAOPB12A, 12K, 12L, 12J and 12C bacillus, through qualitative analysis, was selected as the most productive and the 5 bacteria that constitute it were molecularly identified: PB12A as Bacillus wiedmannii; 12K as B. paramycoides; 12L as Lysinibacillus fusiformis; 12J as B. paramycoides and 12C as Lysinibacillus fusiformis. This work allows to affirm that it is possible to propagate B. Excelsa through the minicutting technique helped by rhizobacteria and contribute to reduce the pressure in the forest, with recovery of degraded areas, increase commercial production and support small producers in the B. Excelsa wood and nut production chain.El objetivo de este trabajo fue analizar la propagación vegetativa de Bertholletia excelsa mediante la técnica de mini esquejes, con la ayuda de mezclas de rizobacterias. La metodología utilizada se basó en la colecta de raíces de B. excelsa, a partir de las cuales se bioprospeccionaron rizobacterias. A partir de ellos formamos mezclas, para la inoculación de mini esquejes en las siembras en tubos que contenían sustrato comercial, a los 90 días se desenterraron para recolectar los datos. El experimento se condujo en un diseño completamente al azar, que constó de 6 tratamientos (5 mezclas y testigos) con 6 repeticiones, con 1 mini esqueje por cada repetición. Los datos, en un universo de 36 unidades de investigación, fueron puestos a disposición en una tabla de excel. Las variables analizadas fueron: supervivencia, formación de callos, formación y longitud de raíces en mini esquejes. Según MINITAB, la supervivencia de mini esquejes tratados con rizobacterias x testigos mostró diferencia significativa por la prueba de KRUSKAL-WALLIS (p-0.01), para las demás variables no hubo variación significativa. La mezcla MAO5PB, compuesta por los bacilos MAOPB12A, 12K, 12L, 12J y 12C, mediante análisis cualitativo, se seleccionó como la más productiva y se identificaron molecularmente las 5 bacterias que la constituyen: PB12A como Bacillus wiedmannii; 12K como B. paramycoides; 12L como Lysinibacillus fusiformis; 12J como B. paramycoides y 12C como Lysinibacillus fusiformis. Este trabajo de investigación permite afirmar que es posible propagar B. excelsa a través de la técnica de mini esquejes ayudado por rizobacterias y, contribuir a reducir la presión en el bosque, con la recuperación de áreas degradadas, aumentar la producción comercial y favorecer pequeños productores en la cadena productiva de madera y frutos secos de B. Excelsa.O objetivo deste trabalho foi analisar a propagação vegetativa de Bertholletia excelsa através da técnica de miniestaquia, com auxílio de mixes de rizobactérias. A metodologia utilizada, se deu a partir da coleta de raízes da B. excelsa, destas foram bioprospectadas rizobactérias. Delas formamos os mixes, para inoculação de miniestacas nos plantios em tubetes contendo substrato comercial, depois de 90 dias foram desenterradas para se levantar os dados. O experimento foi conduzido em delineamento inteiramente casualizado, constituído de 6 tratamentos (5 mixes e testemunhas) com 6 repetições, sendo 1 miniestaca para cada repetição. Os dados foram disponibilizados em tabela no excel. As variáveis analisadas foram: sobrevivência, formação de calos, formação e comprimento das raízes das miniestacas. De acordo com o MINITAB, a sobrevivência das miniestacas tratadas com rizobactérias x testemunhas apresentaram diferença significativa pelo teste de KRUSKAL-WALLIS (p-0,01), nas outras variáveis não houve variação significativa. O mix MAO5PB, constituído pelos bacilos MAOPB12A, 12K, 12L, 12J e 12C, através de análise qualitativa, foi selecionado como o mais produtivo e as 5 bactérias que o constituem foram identificadas molecularmente: PB12A como Bacillus wiedmannii; 12K como B. paramycoides; 12L como Lysinibacillus fusiformis; 12J como B. paramycoides e 12C como Lysinibacillus fusiformis. Este trabalho permite afirmar que é possível propagar B. Excelsa através da técnica de miniestaquia auxiliada por rizobactérias e contribuir para diminuir a pressão na floresta, com a recuperação de áreas degradas, aumentar a produção comercial e favorecer os pequenos produtores na cadeia de produção de madeira e castanha da B. Excelsa.Research, Society and Development2023-06-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/4194710.33448/rsd-v12i6.41947Research, Society and Development; Vol. 12 No. 6; e6612641947Research, Society and Development; Vol. 12 Núm. 6; e6612641947Research, Society and Development; v. 12 n. 6; e66126419472525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/41947/34030Copyright (c) 2023 Júlio Cézar Delfino Ribeiro; Rogério Eije Hanada; Suely de Souza Costahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessRibeiro, Júlio Cézar Delfino Hanada, Rogério Eije Costa, Suely de Souza 2023-07-06T11:16:27Zoai:ojs.pkp.sfu.ca:article/41947Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2023-07-06T11:16:27Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Vegetative propagation by mini-cuttings of Brazil nut “Bertholletia excelsa Bonpl” with the aid of rhizobacteria mixes Propagación vegetativa por miniesquejes de nuez de Brasil “Bertholletia excelsa Bonpl” con la ayuda de mezclas de rizobacterias Propagação vegetativa por miniestacas de Castanha-do-Brasil “Bertholletia excelsa Bonpl” com auxílio de mixes de rizobactérias |
title |
Vegetative propagation by mini-cuttings of Brazil nut “Bertholletia excelsa Bonpl” with the aid of rhizobacteria mixes |
spellingShingle |
Vegetative propagation by mini-cuttings of Brazil nut “Bertholletia excelsa Bonpl” with the aid of rhizobacteria mixes Ribeiro, Júlio Cézar Delfino Prospecção Inoculação Castanha-do-Brasil. Prospección Inoculación Nuez de Brasil. Prospecting Inoculation Brazil nut. |
title_short |
Vegetative propagation by mini-cuttings of Brazil nut “Bertholletia excelsa Bonpl” with the aid of rhizobacteria mixes |
title_full |
Vegetative propagation by mini-cuttings of Brazil nut “Bertholletia excelsa Bonpl” with the aid of rhizobacteria mixes |
title_fullStr |
Vegetative propagation by mini-cuttings of Brazil nut “Bertholletia excelsa Bonpl” with the aid of rhizobacteria mixes |
title_full_unstemmed |
Vegetative propagation by mini-cuttings of Brazil nut “Bertholletia excelsa Bonpl” with the aid of rhizobacteria mixes |
title_sort |
Vegetative propagation by mini-cuttings of Brazil nut “Bertholletia excelsa Bonpl” with the aid of rhizobacteria mixes |
author |
Ribeiro, Júlio Cézar Delfino |
author_facet |
Ribeiro, Júlio Cézar Delfino Hanada, Rogério Eije Costa, Suely de Souza |
author_role |
author |
author2 |
Hanada, Rogério Eije Costa, Suely de Souza |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Ribeiro, Júlio Cézar Delfino Hanada, Rogério Eije Costa, Suely de Souza |
dc.subject.por.fl_str_mv |
Prospecção Inoculação Castanha-do-Brasil. Prospección Inoculación Nuez de Brasil. Prospecting Inoculation Brazil nut. |
topic |
Prospecção Inoculação Castanha-do-Brasil. Prospección Inoculación Nuez de Brasil. Prospecting Inoculation Brazil nut. |
description |
The aim of this work was to analyze the vegetative propagation of Bertholletia excelsa through the mini-cutting technique, with the aid of rhizobacteria mixes. The methodology used was based on the collection of roots of B. excelsa, from which rhizobacteria were bioprospected. From them we formed mixes, for inoculation of mini-cuttings in the plantings in tubes containing commercial substrate, after 90 days they were dug up to collect the data. The experiment was conducted in a completely randomized design, consisting of 6 treatments (5 mixes and controls) with 6 replications, with 1 mini-cutting for each replication. The data were made available in an excel table. The variables analyzed were: survival, callus formation, formation and length of mini-cutting roots. According to MINITAB, the survival of mini-cuttings treated with rhizobacteria x controls presented a significant difference by the KRUSKAL-WALLIS test (p-0.01), for the other variables there was no significant variation. The MAO5PB mix, consisting of MAOPB12A, 12K, 12L, 12J and 12C bacillus, through qualitative analysis, was selected as the most productive and the 5 bacteria that constitute it were molecularly identified: PB12A as Bacillus wiedmannii; 12K as B. paramycoides; 12L as Lysinibacillus fusiformis; 12J as B. paramycoides and 12C as Lysinibacillus fusiformis. This work allows to affirm that it is possible to propagate B. Excelsa through the minicutting technique helped by rhizobacteria and contribute to reduce the pressure in the forest, with recovery of degraded areas, increase commercial production and support small producers in the B. Excelsa wood and nut production chain. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-06-08 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/41947 10.33448/rsd-v12i6.41947 |
url |
https://rsdjournal.org/index.php/rsd/article/view/41947 |
identifier_str_mv |
10.33448/rsd-v12i6.41947 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/41947/34030 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 Júlio Cézar Delfino Ribeiro; Rogério Eije Hanada; Suely de Souza Costa https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 Júlio Cézar Delfino Ribeiro; Rogério Eije Hanada; Suely de Souza Costa https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 12 No. 6; e6612641947 Research, Society and Development; Vol. 12 Núm. 6; e6612641947 Research, Society and Development; v. 12 n. 6; e6612641947 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052625556865024 |