In vitro antifungal activity of Mikania cf hirsutissima ethanolic extract against Candida albicans isolates

Detalhes bibliográficos
Autor(a) principal: Resende, Carlos Ananias Aparecido
Data de Publicação: 2022
Outros Autores: Meri Junior, Angelo Elias, Castro, Adrielle Pieve de, Bittencourt, Wanderley José Mantovani, Resende, Karina Aparecida, Marcondes, Helcio Cassemiro
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/28494
Resumo: Mikania hirsutíssima (family), commonly known in Brazil by the name of "cipó-cabeludo", is widely used in folk medicine. The antimicrobial activities have already been investigated, however, no studies have been carried out to evaluate the plant's antifungal activities. Therefore, the objective of this work was to evaluate the antifungal activity of Mikania cf hirsutissima extract in clinical isolates and standard strains of Candida albicans resistant to fluconazole and itraconazole. After the collection of Mikania cf hirsutissima, it was dried, crushed and weighed, leaving the material on waste in absolute alcohol. After the rest, the crude extract of the stem and leaf was obtained. Clinical isolates of Candida albicans and standard strains were used. Different ethanolic concentrations of leaves and stems were used. To determine the in vitro antioxidant activity of the ethanol extract, the DPPH free radical method was applied. The antifungal activity of ethanolic extracts in Candida albicans strains showed satisfactory results, where the 100 mg/mL stem extract showed average halos of 4.5 mm. All strains were resistant to the antifungals itraconazole and fluconazole. The extract concentrations tested for the evaluation of antioxidant activity showed antioxidant capacity compared to the standard of BHA. The results showed antifungal activity in Candida albicans isolates, where all parts showed fungicidal potential, even in those resistant to Fluconazole and Itraconazole. Thus, the positioning of these compounds in clinical practice may contribute to expand the possibilities of treating infections caused by this species of yeast.
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spelling In vitro antifungal activity of Mikania cf hirsutissima ethanolic extract against Candida albicans isolatesActividad antifúngica in vitro del extracto etanólico de Mikania cf hirsutissima contra los aislados de Candida albicansAtividade antifúngica in vitro do extrato etanólico de Mikania cf hirsutissima contra isolados de Candida albicansCipó cabeludoCandidíaseAtividade antifúngicaConcentração inibitória mínima.Enredadera peludaCandidiasisActividad antifúngicaConcentración mínima inhibitoria.Hairy vineCandidiasisAntifungal activityMinimum inhibitory concentration.Mikania hirsutíssima (family), commonly known in Brazil by the name of "cipó-cabeludo", is widely used in folk medicine. The antimicrobial activities have already been investigated, however, no studies have been carried out to evaluate the plant's antifungal activities. Therefore, the objective of this work was to evaluate the antifungal activity of Mikania cf hirsutissima extract in clinical isolates and standard strains of Candida albicans resistant to fluconazole and itraconazole. After the collection of Mikania cf hirsutissima, it was dried, crushed and weighed, leaving the material on waste in absolute alcohol. After the rest, the crude extract of the stem and leaf was obtained. Clinical isolates of Candida albicans and standard strains were used. Different ethanolic concentrations of leaves and stems were used. To determine the in vitro antioxidant activity of the ethanol extract, the DPPH free radical method was applied. The antifungal activity of ethanolic extracts in Candida albicans strains showed satisfactory results, where the 100 mg/mL stem extract showed average halos of 4.5 mm. All strains were resistant to the antifungals itraconazole and fluconazole. The extract concentrations tested for the evaluation of antioxidant activity showed antioxidant capacity compared to the standard of BHA. The results showed antifungal activity in Candida albicans isolates, where all parts showed fungicidal potential, even in those resistant to Fluconazole and Itraconazole. Thus, the positioning of these compounds in clinical practice may contribute to expand the possibilities of treating infections caused by this species of yeast.Mikania hirsutíssima (familia), comúnmente conocida en Brasil con el nombre de "cipó-cabeludo", es ampliamente utilizada en la medicina popular. Las actividades antimicrobianas ya han sido investigadas, sin embargo, no se han realizado estudios para evaluar las actividades antifúngicas de la planta. Por tanto, el objetivo de este trabajo fue evaluar la actividad antifúngica del extracto de Mikania cf hirsutissima en aislados clínicos y cepas estándar de Candida albicans resistentes a fluconazol e itraconazol. Después de la colecta de Mikania cf hirsutissima, se secó, trituró y pesó, dejando el material de desecho en alcohol absoluto. Luego del reposo se obtuvo el extracto crudo de tallo y hoja. Se utilizaron aislados clínicos de Candida albicans y cepas estándar. Se utilizaron diferentes concentraciones etanólicas de hojas y tallos. Para determinar la actividad antioxidante in vitro del extracto etanólico se aplicó el método de radicales libres DPPH. La actividad antifúngica de los extractos etanólicos en cepas de Candida albicans mostró resultados satisfactorios, donde el extracto de tallo de 100 mg/mL presentó halos promedio de 4,5 mm. Todas las cepas fueron resistentes a los antifúngicos itraconazol y fluconazol. Las concentraciones de extracto probadas para la evaluación de la actividad antioxidante mostraron capacidad antioxidante en comparación con el estándar de BHA. Los resultados mostraron actividad antifúngica en aislamientos de Candida albicans, donde todas las partes mostraron potencial fungicida, incluso en aquellas resistentes a Fluconazol e Itraconazol. Así, el posicionamiento de estos compuestos en la práctica clínica puede contribuir a ampliar las posibilidades de tratamiento de infecciones causadas por esta especie de levadura.Mikania hirsutíssima (família), comumente conhecida no Brasil pelo nome de "cipó-cabeludo", é amplamente utilizada na medicina popular. As atividades antimicrobianas já foram investigadas, entretanto, nenhum estudo foi realizado para avaliar atividades antifúngicas da planta. Portanto, o objetivo deste trabalho foi avaliar a atividade antifúngica do extrato de Mikania cf hirsutissima em isolados clínicos e cepas padrão de Candida albicans resistentes ao fluconazol e itraconazol. Após a coleta da Mikania cf hirsutissima foi realizada a secagem, trituração e pesagem, deixando o material em refugo em álcool absoluto. Posteriormente ao descanso ocorreu a obtenção do extrato bruto do caule e da folha. Foram utilizados isolados clínicos de Candida albicans e cepas padrão. Diferentes concentrações etanólicas de folhas e caule foram utilizadas. Para determinar a atividade antioxidante in vitro do extrato etanólico aplicou-se o método do radical livre DPPH. A atividade antifúngica dos extratos etanólicos em cepas de Candida albicans apresentou resultados satisfatórios, onde o extrato do caule de 100 mg/mL apresentou halos médios de 4,5 mm. Todas as cepas eram resistentes aos antifúngicos itraconazol e fluconazol. As concentrações de extrato testadas para avaliação da atividade antioxidante mostraram capacidade antioxidante em comparação com o padrão de BHA. Os resultados demonstraram atividade antifúngica em isolados de Candida albicans, onde todas as partes apresentaram potencial fungicida, mesmo naqueles resistentes ao Fluconazol e Itraconazol. Assim, o posicionamento desses compostos na prática clínica pode contribuir para ampliar as possibilidades de tratamento de infecções causadas por essa espécie de leveduras.Research, Society and Development2022-04-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2849410.33448/rsd-v11i5.28494Research, Society and Development; Vol. 11 No. 5; e52811528494Research, Society and Development; Vol. 11 Núm. 5; e52811528494Research, Society and Development; v. 11 n. 5; e528115284942525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/28494/24835Copyright (c) 2022 Carlos Ananias Aparecido Resende; Angelo Elias Meri Junior; Adrielle Pieve de Castro; Wanderley José Mantovani Bittencourt; Karina Aparecida Resende; Helcio Cassemiro Marcondeshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessResende, Carlos Ananias AparecidoMeri Junior, Angelo Elias Castro, Adrielle Pieve de Bittencourt, Wanderley José MantovaniResende, Karina Aparecida Marcondes, Helcio Cassemiro2022-04-17T18:18:56Zoai:ojs.pkp.sfu.ca:article/28494Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:45:52.732725Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv In vitro antifungal activity of Mikania cf hirsutissima ethanolic extract against Candida albicans isolates
Actividad antifúngica in vitro del extracto etanólico de Mikania cf hirsutissima contra los aislados de Candida albicans
Atividade antifúngica in vitro do extrato etanólico de Mikania cf hirsutissima contra isolados de Candida albicans
title In vitro antifungal activity of Mikania cf hirsutissima ethanolic extract against Candida albicans isolates
spellingShingle In vitro antifungal activity of Mikania cf hirsutissima ethanolic extract against Candida albicans isolates
Resende, Carlos Ananias Aparecido
Cipó cabeludo
Candidíase
Atividade antifúngica
Concentração inibitória mínima.
Enredadera peluda
Candidiasis
Actividad antifúngica
Concentración mínima inhibitoria.
Hairy vine
Candidiasis
Antifungal activity
Minimum inhibitory concentration.
title_short In vitro antifungal activity of Mikania cf hirsutissima ethanolic extract against Candida albicans isolates
title_full In vitro antifungal activity of Mikania cf hirsutissima ethanolic extract against Candida albicans isolates
title_fullStr In vitro antifungal activity of Mikania cf hirsutissima ethanolic extract against Candida albicans isolates
title_full_unstemmed In vitro antifungal activity of Mikania cf hirsutissima ethanolic extract against Candida albicans isolates
title_sort In vitro antifungal activity of Mikania cf hirsutissima ethanolic extract against Candida albicans isolates
author Resende, Carlos Ananias Aparecido
author_facet Resende, Carlos Ananias Aparecido
Meri Junior, Angelo Elias
Castro, Adrielle Pieve de
Bittencourt, Wanderley José Mantovani
Resende, Karina Aparecida
Marcondes, Helcio Cassemiro
author_role author
author2 Meri Junior, Angelo Elias
Castro, Adrielle Pieve de
Bittencourt, Wanderley José Mantovani
Resende, Karina Aparecida
Marcondes, Helcio Cassemiro
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Resende, Carlos Ananias Aparecido
Meri Junior, Angelo Elias
Castro, Adrielle Pieve de
Bittencourt, Wanderley José Mantovani
Resende, Karina Aparecida
Marcondes, Helcio Cassemiro
dc.subject.por.fl_str_mv Cipó cabeludo
Candidíase
Atividade antifúngica
Concentração inibitória mínima.
Enredadera peluda
Candidiasis
Actividad antifúngica
Concentración mínima inhibitoria.
Hairy vine
Candidiasis
Antifungal activity
Minimum inhibitory concentration.
topic Cipó cabeludo
Candidíase
Atividade antifúngica
Concentração inibitória mínima.
Enredadera peluda
Candidiasis
Actividad antifúngica
Concentración mínima inhibitoria.
Hairy vine
Candidiasis
Antifungal activity
Minimum inhibitory concentration.
description Mikania hirsutíssima (family), commonly known in Brazil by the name of "cipó-cabeludo", is widely used in folk medicine. The antimicrobial activities have already been investigated, however, no studies have been carried out to evaluate the plant's antifungal activities. Therefore, the objective of this work was to evaluate the antifungal activity of Mikania cf hirsutissima extract in clinical isolates and standard strains of Candida albicans resistant to fluconazole and itraconazole. After the collection of Mikania cf hirsutissima, it was dried, crushed and weighed, leaving the material on waste in absolute alcohol. After the rest, the crude extract of the stem and leaf was obtained. Clinical isolates of Candida albicans and standard strains were used. Different ethanolic concentrations of leaves and stems were used. To determine the in vitro antioxidant activity of the ethanol extract, the DPPH free radical method was applied. The antifungal activity of ethanolic extracts in Candida albicans strains showed satisfactory results, where the 100 mg/mL stem extract showed average halos of 4.5 mm. All strains were resistant to the antifungals itraconazole and fluconazole. The extract concentrations tested for the evaluation of antioxidant activity showed antioxidant capacity compared to the standard of BHA. The results showed antifungal activity in Candida albicans isolates, where all parts showed fungicidal potential, even in those resistant to Fluconazole and Itraconazole. Thus, the positioning of these compounds in clinical practice may contribute to expand the possibilities of treating infections caused by this species of yeast.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-15
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/28494
10.33448/rsd-v11i5.28494
url https://rsdjournal.org/index.php/rsd/article/view/28494
identifier_str_mv 10.33448/rsd-v11i5.28494
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/28494/24835
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 11 No. 5; e52811528494
Research, Society and Development; Vol. 11 Núm. 5; e52811528494
Research, Society and Development; v. 11 n. 5; e52811528494
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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