Chemical composition, antimicrobial and antioxidant activity of leaves essential oil the Myrcia palustris DC. (MYRTACEAE)
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Research, Society and Development |
| Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/13303 |
Resumo: | Brazil has the greatest biodiversity in the world and in this context, its native species become an alternative in the search for bioactive molecules for bioprospecting antimicrobials and natural antioxidants. Belonging to the Myrtaceae family, the species Myrcia palustris DC, popularly known as pintagueira-do-mato, does not present studies regarding its biological activities and chemical composition. Therefore, the objective of this work was to determine the chemical composition of the essential oil of M. palustris by gas chromatography coupled with mass spectrometry (GC-MS), to evaluate its antimicrobial activity by the broth microdilution technique and the antioxidant activity by the broth technique. 2,2-diphenyl-1-picryl-hydrazyl (DPPH). The total yield from the essential oil hydrodistillation technique was 0.35%. The GC-MS revealed the presence of 28 compounds, the majority being from the sesquiterpenes class. Antimicrobial activity was observed for all Gram-positive bacteria (Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus) with the exception of Staphyloccocus epidermidis. Regarding Gram-negatives, there was an inhibitory activity only for Shigella flexneri. For yeast Candida albicans, no inhibitory or fungicidal activity was observed. The essential oil showed a capacity to reduce DPPH radicals up to 82.81%, confirming its antioxidant potential. It is suggested that the antimicrobial and antioxidant action present in the essential oil of M. palustris is related to the presence of the major compounds α-Guaieno (25.89%), α-Bulnesene (13.39%) and β-Selinene (4, 76%). |
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Chemical composition, antimicrobial and antioxidant activity of leaves essential oil the Myrcia palustris DC. (MYRTACEAE)Composición química, actividad antimicrobiana y antioxidante del aceite esencial de hojas de Myrcia palustris DC. (MYRTACEAE)Composição química, atividade antimicrobiana e antioxidante do óleo essencial de folhas Myrcia palustris DC. (MYRTACEAE)Atividades biológicasPitangueira-do-matoSubstâncias bioativasDPPH.Actividades biológicasPitangueira-do-matoSustancias bioactivasDPPH.Biological activitiesPitangueira-do-matoBioactive substancesDPPH.Brazil has the greatest biodiversity in the world and in this context, its native species become an alternative in the search for bioactive molecules for bioprospecting antimicrobials and natural antioxidants. Belonging to the Myrtaceae family, the species Myrcia palustris DC, popularly known as pintagueira-do-mato, does not present studies regarding its biological activities and chemical composition. Therefore, the objective of this work was to determine the chemical composition of the essential oil of M. palustris by gas chromatography coupled with mass spectrometry (GC-MS), to evaluate its antimicrobial activity by the broth microdilution technique and the antioxidant activity by the broth technique. 2,2-diphenyl-1-picryl-hydrazyl (DPPH). The total yield from the essential oil hydrodistillation technique was 0.35%. The GC-MS revealed the presence of 28 compounds, the majority being from the sesquiterpenes class. Antimicrobial activity was observed for all Gram-positive bacteria (Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus) with the exception of Staphyloccocus epidermidis. Regarding Gram-negatives, there was an inhibitory activity only for Shigella flexneri. For yeast Candida albicans, no inhibitory or fungicidal activity was observed. The essential oil showed a capacity to reduce DPPH radicals up to 82.81%, confirming its antioxidant potential. It is suggested that the antimicrobial and antioxidant action present in the essential oil of M. palustris is related to the presence of the major compounds α-Guaieno (25.89%), α-Bulnesene (13.39%) and β-Selinene (4, 76%).Brasil tiene la mayor biodiversidad del mundo y en este contexto, sus especies nativas se convierten en una alternativa en la búsqueda de moléculas bioactivas para bioprospección de antimicrobianos y antioxidantes naturales. Perteneciente a la familia Myrtaceae, la especie Myrcia palustris DC, conocida popularmente como pintagueira-do-mato, no presenta estudios sobre sus actividades biológicas y composición química. Por tanto, el objetivo de este trabajo fue determinar la composición química del aceite esencial de M. palustris mediante cromatografía de gases acoplada a espectrometría de masas (GC-MS), para evaluar su actividad antimicrobiana mediante la técnica de microdilución en caldo y la actividad antioxidante mediante la técnica 2,2-difenil-1-picril-hidrazilo (DPPH). El rendimiento total de la técnica de hidrodestilación de aceites esenciales fue del 0,35%. CG-EM reveló la presencia de 28 compuestos, la mayoría de los cuales pertenecen a la clase de sesquiterpenos. Se observó actividad antimicrobiana para todas las bacterias grampositivas (Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus) con la excepción de Staphyloccocus epidermidis. En cuanto a los gram negativos, hubo actividad inhibidora solo para Shigella flexneri. Para la levadura Candida albicans, no se observó actividad inhibidora o fungicida. El aceite esencial mostró capacidad para reducir los radicales DPPH hasta en un 82,81%, lo que confirma su potencial antioxidante. Se sugiere que la acción antimicrobiana y antioxidante presente en el aceite esencial de M. palustris está relacionada con la presencia de los compuestos principales α-Guaieno (25,89%), α-Bulneseno (13,39%) y β-Selineno (4, 76). %).O Brasil apresenta a maior biodiversidade no mundo e neste contexto, suas espécies nativas tornam-se uma alternativa na busca por moléculas bioativas para bioprospecção de antimicrobianos e antioxidantes naturais. Pertencente à família Myrtaceae, a espécie Myrcia palustris DC, conhecida popularmente como pintagueira-do-mato, não apresenta estudos referente às suas atividades biológicas e composição química. Diante disso, o objetivo deste trabalho foi determinar a composição química do óleo essencial de M. palustris por cromatografia gasosa acoplada à espectrometria de massas (CG-EM), avaliar sua atividade antimicrobiana pela técnica de microdiluição em caldo e a atividade antioxidante pela técnica de 2,2-difenil-1-picril-hidrazila (DPPH). O rendimento total a partir da extração pela técnica de hidrodestilação do óleo essencial foi de 0,35%. A CG-EM revelou a presença de 28 compostos, sendo a maioria da classe dos sesquiterpenos. A atividade antimicrobiana foi observada para todas as bactérias Gram-positivas (Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus) com exceção de Staphyloccocus epidermidis. Em relação às Gram-negativas, verificou-se atividade inibitória apenas para Shigella flexneri. Para levedura Candida albicans não foi observada atividade inibitória e nem fungicida. O óleo essencial apresentou capacidade de redução de radicais de DPPH até 82,81%, confirmando seu potencial antioxidante. Sugere-se que a ação antimicrobiana e antioxidante presentes no óleo essencial de M. palustris esteja relacionada à presença dos compostos majoritários α-Guaieno (25,89%), α-Bulneseno (13,39%) e β-Selineno (4,76%).Research, Society and Development2021-03-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1330310.33448/rsd-v10i3.13303Research, Society and Development; Vol. 10 No. 3; e20510313303Research, Society and Development; Vol. 10 Núm. 3; e20510313303Research, Society and Development; v. 10 n. 3; e205103133032525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/13303/11897Copyright (c) 2021 Camila Vogt dos Santos; Ana Paula Mallmann; Adrieli Gorlin Toledo; Débora Marina Bandeira; Willian Ferreira da Costa; Daíse Miranda Ávila Marins; Juliana Moço Corrêa; Fabiana Gisele da Silva Pintohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSantos, Camila Vogt dos Mallmann, Ana PaulaToledo, Adrieli GorlinBandeira, Débora MarinaCosta, Willian Ferreira da Marins, Daíse Miranda Ávila Corrêa, Juliana MoçoPinto, Fabiana Gisele da Silva2021-03-28T12:03:35Zoai:ojs.pkp.sfu.ca:article/13303Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:34:40.596169Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
| dc.title.none.fl_str_mv |
Chemical composition, antimicrobial and antioxidant activity of leaves essential oil the Myrcia palustris DC. (MYRTACEAE) Composición química, actividad antimicrobiana y antioxidante del aceite esencial de hojas de Myrcia palustris DC. (MYRTACEAE) Composição química, atividade antimicrobiana e antioxidante do óleo essencial de folhas Myrcia palustris DC. (MYRTACEAE) |
| title |
Chemical composition, antimicrobial and antioxidant activity of leaves essential oil the Myrcia palustris DC. (MYRTACEAE) |
| spellingShingle |
Chemical composition, antimicrobial and antioxidant activity of leaves essential oil the Myrcia palustris DC. (MYRTACEAE) Santos, Camila Vogt dos Atividades biológicas Pitangueira-do-mato Substâncias bioativas DPPH. Actividades biológicas Pitangueira-do-mato Sustancias bioactivas DPPH. Biological activities Pitangueira-do-mato Bioactive substances DPPH. |
| title_short |
Chemical composition, antimicrobial and antioxidant activity of leaves essential oil the Myrcia palustris DC. (MYRTACEAE) |
| title_full |
Chemical composition, antimicrobial and antioxidant activity of leaves essential oil the Myrcia palustris DC. (MYRTACEAE) |
| title_fullStr |
Chemical composition, antimicrobial and antioxidant activity of leaves essential oil the Myrcia palustris DC. (MYRTACEAE) |
| title_full_unstemmed |
Chemical composition, antimicrobial and antioxidant activity of leaves essential oil the Myrcia palustris DC. (MYRTACEAE) |
| title_sort |
Chemical composition, antimicrobial and antioxidant activity of leaves essential oil the Myrcia palustris DC. (MYRTACEAE) |
| author |
Santos, Camila Vogt dos |
| author_facet |
Santos, Camila Vogt dos Mallmann, Ana Paula Toledo, Adrieli Gorlin Bandeira, Débora Marina Costa, Willian Ferreira da Marins, Daíse Miranda Ávila Corrêa, Juliana Moço Pinto, Fabiana Gisele da Silva |
| author_role |
author |
| author2 |
Mallmann, Ana Paula Toledo, Adrieli Gorlin Bandeira, Débora Marina Costa, Willian Ferreira da Marins, Daíse Miranda Ávila Corrêa, Juliana Moço Pinto, Fabiana Gisele da Silva |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Santos, Camila Vogt dos Mallmann, Ana Paula Toledo, Adrieli Gorlin Bandeira, Débora Marina Costa, Willian Ferreira da Marins, Daíse Miranda Ávila Corrêa, Juliana Moço Pinto, Fabiana Gisele da Silva |
| dc.subject.por.fl_str_mv |
Atividades biológicas Pitangueira-do-mato Substâncias bioativas DPPH. Actividades biológicas Pitangueira-do-mato Sustancias bioactivas DPPH. Biological activities Pitangueira-do-mato Bioactive substances DPPH. |
| topic |
Atividades biológicas Pitangueira-do-mato Substâncias bioativas DPPH. Actividades biológicas Pitangueira-do-mato Sustancias bioactivas DPPH. Biological activities Pitangueira-do-mato Bioactive substances DPPH. |
| description |
Brazil has the greatest biodiversity in the world and in this context, its native species become an alternative in the search for bioactive molecules for bioprospecting antimicrobials and natural antioxidants. Belonging to the Myrtaceae family, the species Myrcia palustris DC, popularly known as pintagueira-do-mato, does not present studies regarding its biological activities and chemical composition. Therefore, the objective of this work was to determine the chemical composition of the essential oil of M. palustris by gas chromatography coupled with mass spectrometry (GC-MS), to evaluate its antimicrobial activity by the broth microdilution technique and the antioxidant activity by the broth technique. 2,2-diphenyl-1-picryl-hydrazyl (DPPH). The total yield from the essential oil hydrodistillation technique was 0.35%. The GC-MS revealed the presence of 28 compounds, the majority being from the sesquiterpenes class. Antimicrobial activity was observed for all Gram-positive bacteria (Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus) with the exception of Staphyloccocus epidermidis. Regarding Gram-negatives, there was an inhibitory activity only for Shigella flexneri. For yeast Candida albicans, no inhibitory or fungicidal activity was observed. The essential oil showed a capacity to reduce DPPH radicals up to 82.81%, confirming its antioxidant potential. It is suggested that the antimicrobial and antioxidant action present in the essential oil of M. palustris is related to the presence of the major compounds α-Guaieno (25.89%), α-Bulnesene (13.39%) and β-Selinene (4, 76%). |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-03-12 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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https://rsdjournal.org/index.php/rsd/article/view/13303 10.33448/rsd-v10i3.13303 |
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https://rsdjournal.org/index.php/rsd/article/view/13303 |
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10.33448/rsd-v10i3.13303 |
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por |
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por |
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https://rsdjournal.org/index.php/rsd/article/view/13303/11897 |
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https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by/4.0 |
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openAccess |
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application/pdf |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development; Vol. 10 No. 3; e20510313303 Research, Society and Development; Vol. 10 Núm. 3; e20510313303 Research, Society and Development; v. 10 n. 3; e20510313303 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
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Universidade Federal de Itajubá (UNIFEI) |
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Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
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