Production of enzymatic complex from agro-industrial biomass and its application in combustible ethanol
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/13705 |
Resumo: | Waste biomass and agro-industrial by-products, for production ethanol, will meet much of the great demand for this product. To reduce costs and optimize production, this study investigated solid-state fermentation (SSF) to obtain crude enzyme complex (CEC) from different agro-industrial biomasses (sugarcane bagasse, corn peel bran, rice straw bran and roasting and ground coffee residue) using cellulolytic fungi. The most promising CEC were evaluated in simultaneous hydrolysis and fermentation (SHF) for ethanol production by Saccharomyces cerevisiae in a culture broth containing sugarcane bagasse treated by steam explosion, and roast and ground coffee residue. In SSF with bioreactor volume of 0.25 L, containing 40 g of the biomass mixture and 40 g of sterile water with resuspended cells (1.0 x108 spores/g of solid medium) and temperature of 30±2 ºC, the strains Trichoderma reesei and Penicilium oxalicum provided the best enzyme activity. The CEC of T. reesei provided a concentration of 7.5 g L-1 of ethanol in a substrate containing treated sugarcane bagasse (60%) and roast and ground coffee residue (40%), under SHF conditions (pH 4.5, 35±2 °C, 48 h). The results obtained in this study show a promising alternative for correct disposal and use of residues and agro-industrial by-products by use in the production of enzymes and lignocellulosic ethanol. |
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Production of enzymatic complex from agro-industrial biomass and its application in combustible ethanolProducción de complejo enzimático a partir de biomasa agroindustrial y su aplicación en etanol combustibleProdução de complexo enzimático a partir de biomassa agroindustrial e sua aplicação em etanol combustívelEtanolResíduosComplexo enzimáticoLignocelulósicoBiomassas.EthanolResiduesEnzyme complexLignocellulosicBiomasses.EtanolResiduosComplejo enzimáticoLignocelulósicoBiomasas.Waste biomass and agro-industrial by-products, for production ethanol, will meet much of the great demand for this product. To reduce costs and optimize production, this study investigated solid-state fermentation (SSF) to obtain crude enzyme complex (CEC) from different agro-industrial biomasses (sugarcane bagasse, corn peel bran, rice straw bran and roasting and ground coffee residue) using cellulolytic fungi. The most promising CEC were evaluated in simultaneous hydrolysis and fermentation (SHF) for ethanol production by Saccharomyces cerevisiae in a culture broth containing sugarcane bagasse treated by steam explosion, and roast and ground coffee residue. In SSF with bioreactor volume of 0.25 L, containing 40 g of the biomass mixture and 40 g of sterile water with resuspended cells (1.0 x108 spores/g of solid medium) and temperature of 30±2 ºC, the strains Trichoderma reesei and Penicilium oxalicum provided the best enzyme activity. The CEC of T. reesei provided a concentration of 7.5 g L-1 of ethanol in a substrate containing treated sugarcane bagasse (60%) and roast and ground coffee residue (40%), under SHF conditions (pH 4.5, 35±2 °C, 48 h). The results obtained in this study show a promising alternative for correct disposal and use of residues and agro-industrial by-products by use in the production of enzymes and lignocellulosic ethanol.Los residuos de biomasa y los subproductos agroindustriales, utilizados para la producción de etanol, cubrirán gran parte de la gran demanda de este producto. Para reducir costos y optimizar la producción, este estudio investigó la fermentación en estado sólido (FES) para obtener complejo enzimático crudo (CEC) a partir de diferentes biomasas agroindustriales (bagazo de caña de azúcar, salvado de cáscara de maíz, salvado de paja de arroz y residuos de café tostado y molido) utilizando hongos celulolíticos. Los CEC más prometedores fueron evaluados en hidrólisis y fermentación simultáneas (HFS) para la producción de etanol por Saccharomyces cerevisiae en caldo de cultivo que contiene bagazo de caña de azúcar tratado por explosión de vapor y residuos de café tostado y molido. En FES, con un volumen de 0.25 L biorreactor, conteniendo 40g de la mezcla de biomasa y 40g de agua estéril con células resuspendidas (1.0 x108 esporas/g de medio sólido) y temperatura de 30±2ºC, las cepas de Trichoderma reesei y Penicilium oxalicum aportaron la mejor actividad enzimática. El CEC de T.reesei proporcionó una concentración de 7.5 g L-1 de etanol en un sustrato que contenía bagazo de caña de azúcar tratado (60%) y residuo de café tostado y molido (40%), en condiciones HFS (pH 4.5; 35±2°C; 48h). Los resultados obtenidos en este estudio mostraron una alternativa prometedora para el correcto destino y uso de residuos y subproductos agroindustriales para la producción de enzimas y etanol lignocelulósico.Os resíduos de biomassa e subprodutos agroindustriais, utilizados para a produção de etanol, atenderá boa parte da grande demanda por esse produto. Para reduzir custos e otimizar a produção, este estudo investigou a fermentação em estado sólido (FES) para obter complexo enzimático bruto (CEB) de diferentes biomassas agroindustriais (bagaço de cana-de-açúcar, farelo de casca de milho, farelo de palha de arroz e torrefação e resíduo de café moído) usando fungos celulolíticos. Os CEB mais promissores foram avaliados em hidrólise e fermentação simultâneas (HFS) para produção de etanol por Saccharomyces cerevisiae em caldo de cultura contendo bagaço de cana tratado por explosão a vapor e resíduo de café torrado e moído. Em FES, com volume de biorreator de 0,25 L, contendo 40g da mistura de biomassa e 40g de água estéril com células ressuspensas (1,0 x108 esporos/g de meio sólido) e temperatura de 30 ± 2ºC, as cepas Trichoderma reesei e Penicilium oxalicum forneceram a melhor atividade enzimática. A CEB de T. reesei proporcionou concentração de 7,5 g L-1 de etanol em substrato contendo bagaço de cana tratado (60%) e resíduo de café torrado e moído (40%), nas condições HFS (pH 4,5; 35±2°C; 48h). Os resultados obtidos neste estudo mostraram uma alternativa promissora para a correta destinação e aproveitamento de resíduos e subprodutos agroindustriais para a produção de enzimas e etanol lignocelulósico.Research, Society and Development2021-06-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1370510.33448/rsd-v10i6.13705Research, Society and Development; Vol. 10 No. 6; e40410613705Research, Society and Development; Vol. 10 Núm. 6; e40410613705Research, Society and Development; v. 10 n. 6; e404106137052525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/13705/14242Copyright (c) 2021 Adam Gonçalves Arruda; Igor Vieira Evangelista; Larissa Soares de Menezes; Janaína Fischer; Vicelma Luiz Cardoso; Líbia Diniz Santos; Carla Zanella Guidinihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessArruda, Adam Gonçalves Evangelista, Igor Vieira Menezes, Larissa Soares de Fischer, Janaína Cardoso, Vicelma Luiz Santos, Líbia Diniz Guidini, Carla Zanella 2021-06-10T22:51:46Zoai:ojs.pkp.sfu.ca:article/13705Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:34:59.267507Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Production of enzymatic complex from agro-industrial biomass and its application in combustible ethanol Producción de complejo enzimático a partir de biomasa agroindustrial y su aplicación en etanol combustible Produção de complexo enzimático a partir de biomassa agroindustrial e sua aplicação em etanol combustível |
title |
Production of enzymatic complex from agro-industrial biomass and its application in combustible ethanol |
spellingShingle |
Production of enzymatic complex from agro-industrial biomass and its application in combustible ethanol Arruda, Adam Gonçalves Etanol Resíduos Complexo enzimático Lignocelulósico Biomassas. Ethanol Residues Enzyme complex Lignocellulosic Biomasses. Etanol Residuos Complejo enzimático Lignocelulósico Biomasas. |
title_short |
Production of enzymatic complex from agro-industrial biomass and its application in combustible ethanol |
title_full |
Production of enzymatic complex from agro-industrial biomass and its application in combustible ethanol |
title_fullStr |
Production of enzymatic complex from agro-industrial biomass and its application in combustible ethanol |
title_full_unstemmed |
Production of enzymatic complex from agro-industrial biomass and its application in combustible ethanol |
title_sort |
Production of enzymatic complex from agro-industrial biomass and its application in combustible ethanol |
author |
Arruda, Adam Gonçalves |
author_facet |
Arruda, Adam Gonçalves Evangelista, Igor Vieira Menezes, Larissa Soares de Fischer, Janaína Cardoso, Vicelma Luiz Santos, Líbia Diniz Guidini, Carla Zanella |
author_role |
author |
author2 |
Evangelista, Igor Vieira Menezes, Larissa Soares de Fischer, Janaína Cardoso, Vicelma Luiz Santos, Líbia Diniz Guidini, Carla Zanella |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Arruda, Adam Gonçalves Evangelista, Igor Vieira Menezes, Larissa Soares de Fischer, Janaína Cardoso, Vicelma Luiz Santos, Líbia Diniz Guidini, Carla Zanella |
dc.subject.por.fl_str_mv |
Etanol Resíduos Complexo enzimático Lignocelulósico Biomassas. Ethanol Residues Enzyme complex Lignocellulosic Biomasses. Etanol Residuos Complejo enzimático Lignocelulósico Biomasas. |
topic |
Etanol Resíduos Complexo enzimático Lignocelulósico Biomassas. Ethanol Residues Enzyme complex Lignocellulosic Biomasses. Etanol Residuos Complejo enzimático Lignocelulósico Biomasas. |
description |
Waste biomass and agro-industrial by-products, for production ethanol, will meet much of the great demand for this product. To reduce costs and optimize production, this study investigated solid-state fermentation (SSF) to obtain crude enzyme complex (CEC) from different agro-industrial biomasses (sugarcane bagasse, corn peel bran, rice straw bran and roasting and ground coffee residue) using cellulolytic fungi. The most promising CEC were evaluated in simultaneous hydrolysis and fermentation (SHF) for ethanol production by Saccharomyces cerevisiae in a culture broth containing sugarcane bagasse treated by steam explosion, and roast and ground coffee residue. In SSF with bioreactor volume of 0.25 L, containing 40 g of the biomass mixture and 40 g of sterile water with resuspended cells (1.0 x108 spores/g of solid medium) and temperature of 30±2 ºC, the strains Trichoderma reesei and Penicilium oxalicum provided the best enzyme activity. The CEC of T. reesei provided a concentration of 7.5 g L-1 of ethanol in a substrate containing treated sugarcane bagasse (60%) and roast and ground coffee residue (40%), under SHF conditions (pH 4.5, 35±2 °C, 48 h). The results obtained in this study show a promising alternative for correct disposal and use of residues and agro-industrial by-products by use in the production of enzymes and lignocellulosic ethanol. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-04 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/13705 10.33448/rsd-v10i6.13705 |
url |
https://rsdjournal.org/index.php/rsd/article/view/13705 |
identifier_str_mv |
10.33448/rsd-v10i6.13705 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/13705/14242 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 6; e40410613705 Research, Society and Development; Vol. 10 Núm. 6; e40410613705 Research, Society and Development; v. 10 n. 6; e40410613705 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052673207304192 |