STUDY OF ENZYMATIC HYDROLYSIS OF SUGAR CANE STRAW FOR THE PRODUCTION OF ETHANOL

Detalhes bibliográficos
Autor(a) principal: Lucarini, Adriana Célia
Data de Publicação: 2017
Outros Autores: Delquiaro, Ana Carolina Tezotto, Vidoca, Ludmila Carolina Prado Tavares, Braz, Rafaela, Martins, Roberta Morelli, Alves, Taís Pereira
Tipo de documento: Artigo
Idioma: por
Título da fonte: The Journal of Engineering and Exact Sciences
Texto Completo: https://periodicos.ufv.br/jcec/article/view/2446941603022017242
Resumo: It was studied the enzymatic hydrolysis of sugarcane straw in order to obtain fermentable sugars. The biomass passed through physical processes (milling, washing and drying), delignification with H2O2 alkaline and hydrolysis. After characterization of the lignocellulosic biomass in natura and delignified it was observed the removal of 50% of the lignin content. The enzymatic activity of T. reesei cellulase and commercial Cellic® CTec2 was quantified. The reaction pH, enzyme concentration and reaction time were studied, obtaining optimum pH 4.0 and 5.5 for T. reesei and Cellic® CTec2, respectively. The highest yield in terms of enzyme concentration was 10 FPU/g of straw for both enzymes and for the time, both enzymes reduced conversion after 24 hours of hydrolysis, possibly due to microbial contamination. Finally, it has been carried out a material balance for the commercial cellulase, resulting in a yield of the enzymatic hydrolysis of the cellulose content of 23.8%.
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spelling STUDY OF ENZYMATIC HYDROLYSIS OF SUGAR CANE STRAW FOR THE PRODUCTION OF ETHANOLESTUDO DA HIDRÓLISE ENZIMÁTICA DA PALHA DA CANA DE AÇÚCAR PARA PRODUÇÃO DE ETANOL DE SEGUNDA GERAÇÃOEthanolBiomassLignocellulosicsEnzymatic HydrolisisCellulaseEtanolBiomassaLignocelulósicaHidrólise enzimáticaCelulaseIt was studied the enzymatic hydrolysis of sugarcane straw in order to obtain fermentable sugars. The biomass passed through physical processes (milling, washing and drying), delignification with H2O2 alkaline and hydrolysis. After characterization of the lignocellulosic biomass in natura and delignified it was observed the removal of 50% of the lignin content. The enzymatic activity of T. reesei cellulase and commercial Cellic® CTec2 was quantified. The reaction pH, enzyme concentration and reaction time were studied, obtaining optimum pH 4.0 and 5.5 for T. reesei and Cellic® CTec2, respectively. The highest yield in terms of enzyme concentration was 10 FPU/g of straw for both enzymes and for the time, both enzymes reduced conversion after 24 hours of hydrolysis, possibly due to microbial contamination. Finally, it has been carried out a material balance for the commercial cellulase, resulting in a yield of the enzymatic hydrolysis of the cellulose content of 23.8%.Estudou-se a hidrólise enzimática da palha da cana para a obtenção de açúcares fermentescíveis. A palha passou por processos físicos (moagem, lavagem e secagem), foi submetida à uma deslignificação com H2O2 em meio alcalino e hidrólise. Após caracterização da biomassa lignocelulósica in natura e deslignificada observou-se remoção de 50% da lignina. Foi quantificada a atividade enzimática da celulase de T. reesei e comercial Cellic® CTec2. Foram estudados pH da reação, concentração de enzima e tempo de reação, obtendo-se pH ótimo 4,0 e 5,5 para a T. reesei e Cellic® CTec2, respectivamente. O maior rendimento em termos de concentração de enzima foi de 10 FPU/g de palha para ambas as enzimas e para o tempo, ambas enzimas apresentaram queda de conversão após 24 horas de hidrólise, possivelmente por contaminação microbiana. Por fim realizou-se o balanço material para a celulase comercial, projetando-se um rendimento do processo de hidrólise enzimática da palha de 23,8%.Universidade Federal de Viçosa - UFV2017-03-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufv.br/jcec/article/view/244694160302201724210.18540/jcecvl3iss2pp242-253The Journal of Engineering and Exact Sciences; Vol. 3 No. 2 (2017); 242-253The Journal of Engineering and Exact Sciences; Vol. 3 Núm. 2 (2017); 242-253The Journal of Engineering and Exact Sciences; v. 3 n. 2 (2017); 242-2532527-1075reponame:The Journal of Engineering and Exact Sciencesinstname:Universidade Federal de Viçosa (UFV)instacron:UFVporhttps://periodicos.ufv.br/jcec/article/view/2446941603022017242/pdfLucarini, Adriana CéliaDelquiaro, Ana Carolina TezottoVidoca, Ludmila Carolina Prado TavaresBraz, RafaelaMartins, Roberta MorelliAlves, Taís Pereirainfo:eu-repo/semantics/openAccess2020-07-14T22:33:26Zoai:ojs.periodicos.ufv.br:article/2317Revistahttp://www.seer.ufv.br/seer/rbeq2/index.php/req2/oai2527-10752527-1075opendoar:2020-07-14T22:33:26The Journal of Engineering and Exact Sciences - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv STUDY OF ENZYMATIC HYDROLYSIS OF SUGAR CANE STRAW FOR THE PRODUCTION OF ETHANOL
ESTUDO DA HIDRÓLISE ENZIMÁTICA DA PALHA DA CANA DE AÇÚCAR PARA PRODUÇÃO DE ETANOL DE SEGUNDA GERAÇÃO
title STUDY OF ENZYMATIC HYDROLYSIS OF SUGAR CANE STRAW FOR THE PRODUCTION OF ETHANOL
spellingShingle STUDY OF ENZYMATIC HYDROLYSIS OF SUGAR CANE STRAW FOR THE PRODUCTION OF ETHANOL
Lucarini, Adriana Célia
Ethanol
Biomass
Lignocellulosics
Enzymatic Hydrolisis
Cellulase
Etanol
Biomassa
Lignocelulósica
Hidrólise enzimática
Celulase
title_short STUDY OF ENZYMATIC HYDROLYSIS OF SUGAR CANE STRAW FOR THE PRODUCTION OF ETHANOL
title_full STUDY OF ENZYMATIC HYDROLYSIS OF SUGAR CANE STRAW FOR THE PRODUCTION OF ETHANOL
title_fullStr STUDY OF ENZYMATIC HYDROLYSIS OF SUGAR CANE STRAW FOR THE PRODUCTION OF ETHANOL
title_full_unstemmed STUDY OF ENZYMATIC HYDROLYSIS OF SUGAR CANE STRAW FOR THE PRODUCTION OF ETHANOL
title_sort STUDY OF ENZYMATIC HYDROLYSIS OF SUGAR CANE STRAW FOR THE PRODUCTION OF ETHANOL
author Lucarini, Adriana Célia
author_facet Lucarini, Adriana Célia
Delquiaro, Ana Carolina Tezotto
Vidoca, Ludmila Carolina Prado Tavares
Braz, Rafaela
Martins, Roberta Morelli
Alves, Taís Pereira
author_role author
author2 Delquiaro, Ana Carolina Tezotto
Vidoca, Ludmila Carolina Prado Tavares
Braz, Rafaela
Martins, Roberta Morelli
Alves, Taís Pereira
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Lucarini, Adriana Célia
Delquiaro, Ana Carolina Tezotto
Vidoca, Ludmila Carolina Prado Tavares
Braz, Rafaela
Martins, Roberta Morelli
Alves, Taís Pereira
dc.subject.por.fl_str_mv Ethanol
Biomass
Lignocellulosics
Enzymatic Hydrolisis
Cellulase
Etanol
Biomassa
Lignocelulósica
Hidrólise enzimática
Celulase
topic Ethanol
Biomass
Lignocellulosics
Enzymatic Hydrolisis
Cellulase
Etanol
Biomassa
Lignocelulósica
Hidrólise enzimática
Celulase
description It was studied the enzymatic hydrolysis of sugarcane straw in order to obtain fermentable sugars. The biomass passed through physical processes (milling, washing and drying), delignification with H2O2 alkaline and hydrolysis. After characterization of the lignocellulosic biomass in natura and delignified it was observed the removal of 50% of the lignin content. The enzymatic activity of T. reesei cellulase and commercial Cellic® CTec2 was quantified. The reaction pH, enzyme concentration and reaction time were studied, obtaining optimum pH 4.0 and 5.5 for T. reesei and Cellic® CTec2, respectively. The highest yield in terms of enzyme concentration was 10 FPU/g of straw for both enzymes and for the time, both enzymes reduced conversion after 24 hours of hydrolysis, possibly due to microbial contamination. Finally, it has been carried out a material balance for the commercial cellulase, resulting in a yield of the enzymatic hydrolysis of the cellulose content of 23.8%.
publishDate 2017
dc.date.none.fl_str_mv 2017-03-19
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.ufv.br/jcec/article/view/2446941603022017242
10.18540/jcecvl3iss2pp242-253
url https://periodicos.ufv.br/jcec/article/view/2446941603022017242
identifier_str_mv 10.18540/jcecvl3iss2pp242-253
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.ufv.br/jcec/article/view/2446941603022017242/pdf
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Viçosa - UFV
publisher.none.fl_str_mv Universidade Federal de Viçosa - UFV
dc.source.none.fl_str_mv The Journal of Engineering and Exact Sciences; Vol. 3 No. 2 (2017); 242-253
The Journal of Engineering and Exact Sciences; Vol. 3 Núm. 2 (2017); 242-253
The Journal of Engineering and Exact Sciences; v. 3 n. 2 (2017); 242-253
2527-1075
reponame:The Journal of Engineering and Exact Sciences
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str The Journal of Engineering and Exact Sciences
collection The Journal of Engineering and Exact Sciences
repository.name.fl_str_mv The Journal of Engineering and Exact Sciences - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv
_version_ 1798313318705790976

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