The biocompatibility of Achyrocline satureioides plant extract over human gingival fibroblasts
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/11902 |
Resumo: | Objective: Achyrocline satureioides “A.satureioides” is a subshrub, widely distributed in South America because of its medicinal properties. Although it is widely used in folk medicine, there is still no approval for its therapeutic use, and its biocompatibility is little explored. This study aimed to evaluate its cytotoxicity and genotoxicity over human gingival fibroblasts (FMM-1). Methodology: Ten different concentration of the glycolic extract of A.satureioides were tested for 5min and 24h of contact with the cells to evaluate its cytotoxicity using the MTT colorimetric assay and to evaluate its genotoxicity using the micronucleus assay. Data were analyzed with one-way ANOVA and Tukey test with a significance level (α=0.05). Results: All tested concentrations of the extract presented cell viability more than 70% and has no significant difference of the control group after 5min and 24h. However, for 5min the 100 mg/mL was cytotoxic and for 24h the 1.56 mg/mL stimulated cell proliferation. For genotoxicity analysis, only the concentration 6.25 mg/mL showed results similar to the control of cell culture in the micronucleus count after 5min and 24h. Conclusions: The glycolic extract of A.satureioides doesn’t have cytotoxic and genotoxic effects in concentrations up to 6.25 mg/mL, but in high concentrations it is considered genotoxic. |
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The biocompatibility of Achyrocline satureioides plant extract over human gingival fibroblasts La biocompatibilidad del extracto de la planta Achyrocline satureioides en fibroblastos gingivales humanosA biocompatibilidade do extrato da planta Achyrocline satureioides sobre fibroblastos gengivais humanosFibroblasts Plant extractsPhytotherapy. FibroblastosExtractos vegetalesFitoterapia.FibroblastosExtratos vegetaisFitoterapia.Objective: Achyrocline satureioides “A.satureioides” is a subshrub, widely distributed in South America because of its medicinal properties. Although it is widely used in folk medicine, there is still no approval for its therapeutic use, and its biocompatibility is little explored. This study aimed to evaluate its cytotoxicity and genotoxicity over human gingival fibroblasts (FMM-1). Methodology: Ten different concentration of the glycolic extract of A.satureioides were tested for 5min and 24h of contact with the cells to evaluate its cytotoxicity using the MTT colorimetric assay and to evaluate its genotoxicity using the micronucleus assay. Data were analyzed with one-way ANOVA and Tukey test with a significance level (α=0.05). Results: All tested concentrations of the extract presented cell viability more than 70% and has no significant difference of the control group after 5min and 24h. However, for 5min the 100 mg/mL was cytotoxic and for 24h the 1.56 mg/mL stimulated cell proliferation. For genotoxicity analysis, only the concentration 6.25 mg/mL showed results similar to the control of cell culture in the micronucleus count after 5min and 24h. Conclusions: The glycolic extract of A.satureioides doesn’t have cytotoxic and genotoxic effects in concentrations up to 6.25 mg/mL, but in high concentrations it is considered genotoxic.Objetivo: Achyrocline satureioides “A.satureioides” es un subarbusto ampliamente distribuido en América del Sur por sus propiedades medicinales. Aunque es muy utilizado en la medicina popular, todavía no hay aprobación para su uso terapéutico y su biocompatibilidad está poco explorada. Este estudio tuvo como objetivo evaluar su citotoxicidad y genotoxicidad en fibroblastos gingivales humanos (FMM-1). Metodología: Se probaron diez concentraciones diferentes del extracto glicólico de A. satureioides durante 5min y 24h de contacto con las células para evaluar su citotoxicidad mediante el ensayo colorimétrico MTT y su genotoxicidad mediante el ensayo de micronúcleos. Los datos fueron analizados con ANOVA de una vía y prueba de Tukey con nivel de significancia (α = 0.05). Resultados: Todas las concentraciones probadas del extracto mostraron una viabilidad celular superior al 70% y no hubo diferencias significativas en el grupo de control después de 5 min y 24 h. Sin embargo, durante 5 minutos a 100 mg / ml fue citotóxico y durante 24 horas, 1,56 mg / ml estimuló la proliferación celular. Para el análisis de genotoxicidad, solo la concentración 6.25 mg / mL presentó resultados similares al control de cultivo celular en el recuento de micronúcleos a los 5min y 24h. Conclusión: El extracto glicólico de A. satureioides no tiene efectos citotóxicos y genotóxicos en concentraciones de hasta 6.25 mg / mL pero en concentraciones altas se considera genotóxico.Objetivo: Achyrocline satureioides “A.satureioides” é um subarbusto amplamente distribuído na América do Sul por suas propriedades medicinais. Embora seja amplamente utilizado na medicina popular, ainda não há aprovação para seu uso terapêutico e a sua biocompatibilidade é pouco explorada. Este estudo teve como objetivo avaliar sua citotoxicidade e genotoxicidade sobre fibroblastos gengivais humanos (FMM-1). Metodologia: Dez diferentes concentrações do extrato glicólico de A. satureioides foram testadas por 5min e 24h de contato com as células para avaliar sua citotoxicidade pelo ensaio colorimétrico MTT e para avaliar sua genotoxicidade pelo ensaio do micronúcleo. Os dados foram analisados com ANOVA one-way e teste de Tukey com nível de significância (α = 0,05). Resultados: Todas as concentrações testadas do extrato apresentaram viabilidade celular superior a 70% e não houve diferença significativa do grupo controle após 5min e 24h. Porém, por 5min a 100 mg / mL foi citotóxica e por 24h o 1,56 mg / mL estimulou a proliferação celular. Para a análise de genotoxicidade, apenas a concentração 6,25 mg / mL apresentou resultados semelhantes ao controle de cultura de células na contagem de micronúcleos após 5min e 24h. Conclusão: O extrato glicólico de A. satureioides não apresenta efeitos citotóxicos e genotóxicos em concentrações de até 6,25 mg / mL mas em altas concentrações é considerado genotóxico.Research, Society and Development2021-01-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1190210.33448/rsd-v10i1.11902Research, Society and Development; Vol. 10 No. 1; e37610111902Research, Society and Development; Vol. 10 Núm. 1; e37610111902Research, Society and Development; v. 10 n. 1; e376101119022525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/11902/10625Copyright (c) 2021 Elis Cecília Castro Ferreira; Taciano Moreira Gonçalves; Thaís Cristine Pereira; Amjad Abu Hasna; Felipe Eduardo de Oliveira; Adeline Lacerda Jorjão; Samira Esteves Afonso Camargo; Luciane Dias de Oliveira; Marianne Spaldinghttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessFerreira, Elis Cecília Castro Gonçalves, Taciano Moreira Pereira, Thaís Cristine Hasna, Amjad Abu Oliveira, Felipe Eduardo de Jorjão, Adeline Lacerda Camargo, Samira Esteves Afonso Oliveira, Luciane Dias de Spalding, Marianne 2021-02-20T21:19:23Zoai:ojs.pkp.sfu.ca:article/11902Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:33:36.812383Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
The biocompatibility of Achyrocline satureioides plant extract over human gingival fibroblasts La biocompatibilidad del extracto de la planta Achyrocline satureioides en fibroblastos gingivales humanos A biocompatibilidade do extrato da planta Achyrocline satureioides sobre fibroblastos gengivais humanos |
title |
The biocompatibility of Achyrocline satureioides plant extract over human gingival fibroblasts |
spellingShingle |
The biocompatibility of Achyrocline satureioides plant extract over human gingival fibroblasts Ferreira, Elis Cecília Castro Fibroblasts Plant extracts Phytotherapy. Fibroblastos Extractos vegetales Fitoterapia. Fibroblastos Extratos vegetais Fitoterapia. |
title_short |
The biocompatibility of Achyrocline satureioides plant extract over human gingival fibroblasts |
title_full |
The biocompatibility of Achyrocline satureioides plant extract over human gingival fibroblasts |
title_fullStr |
The biocompatibility of Achyrocline satureioides plant extract over human gingival fibroblasts |
title_full_unstemmed |
The biocompatibility of Achyrocline satureioides plant extract over human gingival fibroblasts |
title_sort |
The biocompatibility of Achyrocline satureioides plant extract over human gingival fibroblasts |
author |
Ferreira, Elis Cecília Castro |
author_facet |
Ferreira, Elis Cecília Castro Gonçalves, Taciano Moreira Pereira, Thaís Cristine Hasna, Amjad Abu Oliveira, Felipe Eduardo de Jorjão, Adeline Lacerda Camargo, Samira Esteves Afonso Oliveira, Luciane Dias de Spalding, Marianne |
author_role |
author |
author2 |
Gonçalves, Taciano Moreira Pereira, Thaís Cristine Hasna, Amjad Abu Oliveira, Felipe Eduardo de Jorjão, Adeline Lacerda Camargo, Samira Esteves Afonso Oliveira, Luciane Dias de Spalding, Marianne |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Ferreira, Elis Cecília Castro Gonçalves, Taciano Moreira Pereira, Thaís Cristine Hasna, Amjad Abu Oliveira, Felipe Eduardo de Jorjão, Adeline Lacerda Camargo, Samira Esteves Afonso Oliveira, Luciane Dias de Spalding, Marianne |
dc.subject.por.fl_str_mv |
Fibroblasts Plant extracts Phytotherapy. Fibroblastos Extractos vegetales Fitoterapia. Fibroblastos Extratos vegetais Fitoterapia. |
topic |
Fibroblasts Plant extracts Phytotherapy. Fibroblastos Extractos vegetales Fitoterapia. Fibroblastos Extratos vegetais Fitoterapia. |
description |
Objective: Achyrocline satureioides “A.satureioides” is a subshrub, widely distributed in South America because of its medicinal properties. Although it is widely used in folk medicine, there is still no approval for its therapeutic use, and its biocompatibility is little explored. This study aimed to evaluate its cytotoxicity and genotoxicity over human gingival fibroblasts (FMM-1). Methodology: Ten different concentration of the glycolic extract of A.satureioides were tested for 5min and 24h of contact with the cells to evaluate its cytotoxicity using the MTT colorimetric assay and to evaluate its genotoxicity using the micronucleus assay. Data were analyzed with one-way ANOVA and Tukey test with a significance level (α=0.05). Results: All tested concentrations of the extract presented cell viability more than 70% and has no significant difference of the control group after 5min and 24h. However, for 5min the 100 mg/mL was cytotoxic and for 24h the 1.56 mg/mL stimulated cell proliferation. For genotoxicity analysis, only the concentration 6.25 mg/mL showed results similar to the control of cell culture in the micronucleus count after 5min and 24h. Conclusions: The glycolic extract of A.satureioides doesn’t have cytotoxic and genotoxic effects in concentrations up to 6.25 mg/mL, but in high concentrations it is considered genotoxic. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-18 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/11902 10.33448/rsd-v10i1.11902 |
url |
https://rsdjournal.org/index.php/rsd/article/view/11902 |
identifier_str_mv |
10.33448/rsd-v10i1.11902 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/11902/10625 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 1; e37610111902 Research, Society and Development; Vol. 10 Núm. 1; e37610111902 Research, Society and Development; v. 10 n. 1; e37610111902 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
_version_ |
1797052668633415680 |