Preliminary evaluation of Brazilian green propolis as coadjuvant of a subunit vaccine against lethal leptospirosis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Research, Society and Development |
| Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/20947 |
Resumo: | Vaccination would be preferred to leptospirosis control measures. However, current vaccines are heat killed whole-cell bacterins that generate serovar specific protection and several side effects. Modern molecular assays have revealed antigens that may replace traditional whole-cell vaccines. Among these, LigB protein is surface-exposed outer membrane protein of virulent leptospires and therefore potential target of a protective immune response. Some unsuccessful attempts at using these antigens as vaccines have been reported. However, we believe that immune modulation through alternative adjuvants and co-adjuvants may overcome previous setbacks. In this light, our study aimed to evaluate the protective immune response in hamsters vaccinated with 40 µg of rLigBNI using oil adjuvant (OA), with or without green propolis (GP) as co-adjuvant. Upon a challenge, all groups immunized with rLigBNI, coupled or not with GP, were highly immunogenic and revealed statistically significant (p<0.05) protection of hamsters from lethal leptospirosis. Additional studies are being carried out to assess the optimum dose, protection against heterologous challenge, and vaccine dynamics. |
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Preliminary evaluation of Brazilian green propolis as coadjuvant of a subunit vaccine against lethal leptospirosisEvaluación preliminar del propóleo verde brasileño como coadyuvante de una vacuna de subunidad contra la leptospirosis letalAvaliação preliminar da própolis verde brasileira como coadjuvante de uma vacina de subunidade contra a leptospirose letalLeptospiroseVacinas recombinantsAdjuvantePrópolis verde brasileira.LeptospirosisVacunas recombinantesAdyuvantePropóleo verde brasileño.LeptospirosisRecombinant vaccinesAdjuvantBrazilian green propolis.Vaccination would be preferred to leptospirosis control measures. However, current vaccines are heat killed whole-cell bacterins that generate serovar specific protection and several side effects. Modern molecular assays have revealed antigens that may replace traditional whole-cell vaccines. Among these, LigB protein is surface-exposed outer membrane protein of virulent leptospires and therefore potential target of a protective immune response. Some unsuccessful attempts at using these antigens as vaccines have been reported. However, we believe that immune modulation through alternative adjuvants and co-adjuvants may overcome previous setbacks. In this light, our study aimed to evaluate the protective immune response in hamsters vaccinated with 40 µg of rLigBNI using oil adjuvant (OA), with or without green propolis (GP) as co-adjuvant. Upon a challenge, all groups immunized with rLigBNI, coupled or not with GP, were highly immunogenic and revealed statistically significant (p<0.05) protection of hamsters from lethal leptospirosis. Additional studies are being carried out to assess the optimum dose, protection against heterologous challenge, and vaccine dynamics.La vacunación es una medida preferida para controlar la leptospirosis. Sin embargo, las vacunas actuales son bacterinas, con células enteras muertas por el calor, que generan protección específica de serovariedad y tienen efectos secundarios. Los ensayos moleculares han revelado antígenos que podrían reemplazar a las vacunas tradicionales de células enteras. Entre estos, la proteína de la membrana externa LigB, que está expuesta en la superficie de las leptospiras virulentas, es un objetivo potencial para una respuesta inmune protectora. Se han informado algunos intentos fallidos de utilizar estos antígenos como vacunas. Sin embargo, la modulación inmune a través de adyuvantes y adyuvantes alternativos puede superar los retrocesos previos. Por tanto, nuestro estudio tuvo como objetivo evaluar la respuesta inmunitaria protectora en hámsteres vacunados con 40 µg de rLigBNI utilizando adyuvante oleoso (OA), con o sin propóleo verde (GP) como coadyuvante. Después del desafío, todos los grupos vacunados con rLigBNI, con o sin GP, mostraron inmunogenicidad e inmunoprotección estadísticamente significativas (p <0,05). Se llevarán a cabo estudios adicionales para evaluar la dosis óptima, la protección contra el desafío heterólogo y la dinámica de la vacuna.A vacinação é uma medida preferencial para o controle da leptospirose. No entanto, as vacinas atuais são bacterinas, com células inteiras mortas pelo calor, que geram proteção sorovar-específica e apresentam efeitos colaterais. Ensaios moleculares revelaram antígenos que podem substituir as vacinas de células inteiras tradicionais. Entre estes, a proteína de membrana externa LigB, a qual está exposta na superfície de leptospiras virulentas, é um alvo potencial para uma resposta imune protetora. Algumas tentativas malsucedidas de usar esses antígenos como vacinas já foram relatadas. No entanto, a modulação imunológica por meio de adjuvantes e coadjuvantes alternativos pode superar contratempos anteriores. Diante disso, nosso estudo objetivou avaliar a resposta imune protetora em hamsters vacinados com 40 µg de rLigBNI usando adjuvante oleoso (OA), com ou sem própolis verde (GP) como co-adjuvante. Após o desafio, todos os grupos vacinados com rLigBNI, administrado-se ou não GP, revelaram imunogenicidade e imunoproteção estatisticamente significativa (p <0,05). Estudos adicionais serão realizados para avaliar a dose ideal, a proteção contra o desafio heterólogo e a dinâmica da vacina.Research, Society and Development2021-09-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2094710.33448/rsd-v10i12.20947Research, Society and Development; Vol. 10 No. 12; e521101220945Research, Society and Development; Vol. 10 Núm. 12; e521101220945Research, Society and Development; v. 10 n. 12; e5211012209452525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/20947/18501Copyright (c) 2021 Michel Quevedo Fagundes; Amilton Clair Pinto Seixas Neto; Samuel Rodrigues Felix; Flávia Aleixo Vasconcellos; Geferson Fischer; Marco Alberto Medeiros; Éverton Fagonde da Silvahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessFagundes, Michel QuevedoSeixas Neto, Amilton Clair Pinto Felix, Samuel Rodrigues Vasconcellos, Flávia AleixoFischer, Geferson Medeiros, Marco Alberto Silva, Éverton Fagonde da 2021-11-14T20:26:51Zoai:ojs.pkp.sfu.ca:article/20947Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:40:28.204851Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
| dc.title.none.fl_str_mv |
Preliminary evaluation of Brazilian green propolis as coadjuvant of a subunit vaccine against lethal leptospirosis Evaluación preliminar del propóleo verde brasileño como coadyuvante de una vacuna de subunidad contra la leptospirosis letal Avaliação preliminar da própolis verde brasileira como coadjuvante de uma vacina de subunidade contra a leptospirose letal |
| title |
Preliminary evaluation of Brazilian green propolis as coadjuvant of a subunit vaccine against lethal leptospirosis |
| spellingShingle |
Preliminary evaluation of Brazilian green propolis as coadjuvant of a subunit vaccine against lethal leptospirosis Fagundes, Michel Quevedo Leptospirose Vacinas recombinants Adjuvante Própolis verde brasileira. Leptospirosis Vacunas recombinantes Adyuvante Propóleo verde brasileño. Leptospirosis Recombinant vaccines Adjuvant Brazilian green propolis. |
| title_short |
Preliminary evaluation of Brazilian green propolis as coadjuvant of a subunit vaccine against lethal leptospirosis |
| title_full |
Preliminary evaluation of Brazilian green propolis as coadjuvant of a subunit vaccine against lethal leptospirosis |
| title_fullStr |
Preliminary evaluation of Brazilian green propolis as coadjuvant of a subunit vaccine against lethal leptospirosis |
| title_full_unstemmed |
Preliminary evaluation of Brazilian green propolis as coadjuvant of a subunit vaccine against lethal leptospirosis |
| title_sort |
Preliminary evaluation of Brazilian green propolis as coadjuvant of a subunit vaccine against lethal leptospirosis |
| author |
Fagundes, Michel Quevedo |
| author_facet |
Fagundes, Michel Quevedo Seixas Neto, Amilton Clair Pinto Felix, Samuel Rodrigues Vasconcellos, Flávia Aleixo Fischer, Geferson Medeiros, Marco Alberto Silva, Éverton Fagonde da |
| author_role |
author |
| author2 |
Seixas Neto, Amilton Clair Pinto Felix, Samuel Rodrigues Vasconcellos, Flávia Aleixo Fischer, Geferson Medeiros, Marco Alberto Silva, Éverton Fagonde da |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Fagundes, Michel Quevedo Seixas Neto, Amilton Clair Pinto Felix, Samuel Rodrigues Vasconcellos, Flávia Aleixo Fischer, Geferson Medeiros, Marco Alberto Silva, Éverton Fagonde da |
| dc.subject.por.fl_str_mv |
Leptospirose Vacinas recombinants Adjuvante Própolis verde brasileira. Leptospirosis Vacunas recombinantes Adyuvante Propóleo verde brasileño. Leptospirosis Recombinant vaccines Adjuvant Brazilian green propolis. |
| topic |
Leptospirose Vacinas recombinants Adjuvante Própolis verde brasileira. Leptospirosis Vacunas recombinantes Adyuvante Propóleo verde brasileño. Leptospirosis Recombinant vaccines Adjuvant Brazilian green propolis. |
| description |
Vaccination would be preferred to leptospirosis control measures. However, current vaccines are heat killed whole-cell bacterins that generate serovar specific protection and several side effects. Modern molecular assays have revealed antigens that may replace traditional whole-cell vaccines. Among these, LigB protein is surface-exposed outer membrane protein of virulent leptospires and therefore potential target of a protective immune response. Some unsuccessful attempts at using these antigens as vaccines have been reported. However, we believe that immune modulation through alternative adjuvants and co-adjuvants may overcome previous setbacks. In this light, our study aimed to evaluate the protective immune response in hamsters vaccinated with 40 µg of rLigBNI using oil adjuvant (OA), with or without green propolis (GP) as co-adjuvant. Upon a challenge, all groups immunized with rLigBNI, coupled or not with GP, were highly immunogenic and revealed statistically significant (p<0.05) protection of hamsters from lethal leptospirosis. Additional studies are being carried out to assess the optimum dose, protection against heterologous challenge, and vaccine dynamics. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-09-29 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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https://rsdjournal.org/index.php/rsd/article/view/20947 10.33448/rsd-v10i12.20947 |
| url |
https://rsdjournal.org/index.php/rsd/article/view/20947 |
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10.33448/rsd-v10i12.20947 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
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https://rsdjournal.org/index.php/rsd/article/view/20947/18501 |
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https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by/4.0 |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development; Vol. 10 No. 12; e521101220945 Research, Society and Development; Vol. 10 Núm. 12; e521101220945 Research, Society and Development; v. 10 n. 12; e521101220945 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
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Universidade Federal de Itajubá (UNIFEI) |
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UNIFEI |
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UNIFEI |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
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rsd.articles@gmail.com |
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1797052833942470656 |