Selection of technique for yeast collection from the canine oral cavity
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/30716 |
Resumo: | The first step in the collection of microbiological samples is the selection of techniques to be used, which guarantees a higher yield of microorganisms and a more assertive identification of them. Considering the scarcity of studies related to the presence of yeasts in the canine oral cavity, this work broached the comparison between techniques of collection of the canine oral cavity already used and not yet used in 18 dogs treated at the Hospital Veterinário de Animais de Companhia (UFRRJ) and private clinics of the adjacencies. The purpose of this comparative study is to stimulate research for the standardization of the canine oral microbiome and to expand knowledge about the yeasts present in this microbiota. Therefore, the following instruments were compared for sample collection: swab, filter paper and commercial human toothbrush. The technique that demonstrated the greater use of the sample and less microbial loss was the use of a toothbrush. Therefore, it was concluded that the most effective collection of yeasts from the canine oral cavity was the commercial human toothbrush, recommended for future trials. |
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Selection of technique for yeast collection from the canine oral cavitySelección de técnica para la recoleccion de levaduras de la cavidad oral caninaSeleção de técnica para coleta de leveduras da cavidade oral caninaEstudo ComparativoColeta AmostralMicrobiotaExperimento.Estudio ComparativoColección de MuestraMicrobiotaExperimento.Comparative StudySample CollectionMicrobiotaExperiment.The first step in the collection of microbiological samples is the selection of techniques to be used, which guarantees a higher yield of microorganisms and a more assertive identification of them. Considering the scarcity of studies related to the presence of yeasts in the canine oral cavity, this work broached the comparison between techniques of collection of the canine oral cavity already used and not yet used in 18 dogs treated at the Hospital Veterinário de Animais de Companhia (UFRRJ) and private clinics of the adjacencies. The purpose of this comparative study is to stimulate research for the standardization of the canine oral microbiome and to expand knowledge about the yeasts present in this microbiota. Therefore, the following instruments were compared for sample collection: swab, filter paper and commercial human toothbrush. The technique that demonstrated the greater use of the sample and less microbial loss was the use of a toothbrush. Therefore, it was concluded that the most effective collection of yeasts from the canine oral cavity was the commercial human toothbrush, recommended for future trials.El primer paso en la toma de muestras microbiológicas es la selección de las técnicas a utilizar, lo que garantiza un mejor aprovechamiento de los microorganismos y una identificación más asertiva de los mismos. Considerando la escasez de estudios relacionados con la presencia de levaduras en la cavidad bucal canina, este trabajo abordó la comparación entre técnicas de recolección de la cavidad bucal canina ya utilizadas y aún no utilizadas en 18 perros atendidos en el Hospital Veterinário de Animais de Companhia (UFRRJ) y clínicas privadas de las adyacencias. El propósito de este estudio comparativo es estimular la investigación para la estandarización del microbioma oral canino y ampliar el conocimiento sobre las levaduras presentes en esta microbiota. Por lo tanto, se compararon los siguientes instrumentos para la recolección de muestras: hisopo, papel de filtro y cepillo de dientes humano comercial. La técnica que demostró mayor aprovechamiento de la muestra y menor pérdida microbiana fue el uso de cepillo de dientes. Por lo tanto, se concluyó que el instrumento de recolección más efectivo para la recolección de levaduras de la cavidad bucal canina fue el cepillo dental humano comercial, recomendado para futuros ensayos.O primeiro passo para a coleta de amostras microbiológicas é a seleção entre técnicas a serem utilizadas, o que garante um maior aproveitamento dos micro-organismos e uma identificação mais assertiva dos mesmos. Pensando na escassez de estudos relacionados à presença de leveduras na cavidade oral canina, esse trabalho abordou a comparação entre técnicas de coletas da cavidade oral canina já utilizadas e ainda não utilizadas em 18 cães atendidos no Hospital Veterinário de Animais de Companhia da UFRRJ e clínicas particulares das adjacências. A finalidade desse estudo comparativo é estimular pesquisas para a padronização do microbioma oral canino e ampliar conhecimentos sobre as leveduras presentes nessa microbiota. Para tanto, foram comparados os seguintes instrumentos para colheita de amostras: swab, papel filtro e escova de dente comercial humana. A técnica que demonstrou maior aproveitamento de amostra e menor perda microbiana foi o uso da escova de dentes. Sendo assim, concluiu-se que o instrumento de coleta mais eficaz para a coleta de leveduras da cavidade oral canina foi a escova de dentes comercial humana, recomendada para ensaios futuros.Research, Society and Development2022-06-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/3071610.33448/rsd-v11i8.30716Research, Society and Development; Vol. 11 No. 8; e14911830716Research, Society and Development; Vol. 11 Núm. 8; e14911830716Research, Society and Development; v. 11 n. 8; e149118307162525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/30716/26358Copyright (c) 2022 Clara de Almeida Mendes; Mário Mendes Bonci; Francisco de Assis Baronihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessMendes, Clara de Almeida Bonci, Mário MendesBaroni, Francisco de Assis2022-07-01T13:34:06Zoai:ojs.pkp.sfu.ca:article/30716Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:47:22.993663Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Selection of technique for yeast collection from the canine oral cavity Selección de técnica para la recoleccion de levaduras de la cavidad oral canina Seleção de técnica para coleta de leveduras da cavidade oral canina |
title |
Selection of technique for yeast collection from the canine oral cavity |
spellingShingle |
Selection of technique for yeast collection from the canine oral cavity Mendes, Clara de Almeida Estudo Comparativo Coleta Amostral Microbiota Experimento. Estudio Comparativo Colección de Muestra Microbiota Experimento. Comparative Study Sample Collection Microbiota Experiment. |
title_short |
Selection of technique for yeast collection from the canine oral cavity |
title_full |
Selection of technique for yeast collection from the canine oral cavity |
title_fullStr |
Selection of technique for yeast collection from the canine oral cavity |
title_full_unstemmed |
Selection of technique for yeast collection from the canine oral cavity |
title_sort |
Selection of technique for yeast collection from the canine oral cavity |
author |
Mendes, Clara de Almeida |
author_facet |
Mendes, Clara de Almeida Bonci, Mário Mendes Baroni, Francisco de Assis |
author_role |
author |
author2 |
Bonci, Mário Mendes Baroni, Francisco de Assis |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Mendes, Clara de Almeida Bonci, Mário Mendes Baroni, Francisco de Assis |
dc.subject.por.fl_str_mv |
Estudo Comparativo Coleta Amostral Microbiota Experimento. Estudio Comparativo Colección de Muestra Microbiota Experimento. Comparative Study Sample Collection Microbiota Experiment. |
topic |
Estudo Comparativo Coleta Amostral Microbiota Experimento. Estudio Comparativo Colección de Muestra Microbiota Experimento. Comparative Study Sample Collection Microbiota Experiment. |
description |
The first step in the collection of microbiological samples is the selection of techniques to be used, which guarantees a higher yield of microorganisms and a more assertive identification of them. Considering the scarcity of studies related to the presence of yeasts in the canine oral cavity, this work broached the comparison between techniques of collection of the canine oral cavity already used and not yet used in 18 dogs treated at the Hospital Veterinário de Animais de Companhia (UFRRJ) and private clinics of the adjacencies. The purpose of this comparative study is to stimulate research for the standardization of the canine oral microbiome and to expand knowledge about the yeasts present in this microbiota. Therefore, the following instruments were compared for sample collection: swab, filter paper and commercial human toothbrush. The technique that demonstrated the greater use of the sample and less microbial loss was the use of a toothbrush. Therefore, it was concluded that the most effective collection of yeasts from the canine oral cavity was the commercial human toothbrush, recommended for future trials. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-06-13 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/30716 10.33448/rsd-v11i8.30716 |
url |
https://rsdjournal.org/index.php/rsd/article/view/30716 |
identifier_str_mv |
10.33448/rsd-v11i8.30716 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/30716/26358 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2022 Clara de Almeida Mendes; Mário Mendes Bonci; Francisco de Assis Baroni https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2022 Clara de Almeida Mendes; Mário Mendes Bonci; Francisco de Assis Baroni https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 11 No. 8; e14911830716 Research, Society and Development; Vol. 11 Núm. 8; e14911830716 Research, Society and Development; v. 11 n. 8; e14911830716 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052826648576000 |