Biotechnology: use of available carbon sources on the planet to generate alternatives energy

Detalhes bibliográficos
Autor(a) principal: Costa Junior, Jefferson Alves da
Data de Publicação: 2022
Outros Autores: Vicente, Elisabete José, Marasca , Elza Teresinha Grael, Torres , Fernando Araripe Gonçalves, Moraes , Lidia Maria Pepe de, Bones , Ubiratan Alegransi
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/27904
Resumo: Biotechnology has been an essential tool in the search for solutions and in the optimization of bioprocesses associated with issues of human, plant, animal, energy and also the balance of ecosystems on planet Earth. The objective of this research was to present an unconventional substrate (cellulose), in abundance on the planet, to be used as a substitute source of carbon and energy for biotechnology processes, with the possibility of increasing industrial production of biomass and energy. As basis for the research, an extensive literature review and quantitative and qualitative analyzes were carried out. Genetic Engineering techniques were used to enable the yeast Saccharomyces cerevisiae for partial cellulose degradation, through the use of genetic transformation methods to insert a plasmid carrying the cellobiohydrolase cDNA. It was found that the recombinant and biologically active cellobiohydrolase protein was expressed and excreted in haploid and diploid laboratory yeast strains. The analyzes allowed the visualization of cellulolysis halos around colonies of recombinant strains grown in solid YPD medium with 1% microgranular cellulose. The recombinant clones derived from the haploid lineage yielded in average of 1.70 mg ART/mL, while recombinant clones derived from the diploid lineage produced in average of 2.05 mg ART/mL.
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spelling Biotechnology: use of available carbon sources on the planet to generate alternatives energyBiotecnología: uso de las fuentes de carbono disponibles en el planeta para generar energías alternativasBiotecnologia: uso de fontes de carbono disponíveis no planeta para geração de energia alternativaBioetanolCelobiohidrolaseCeluloseEngenharia genéticaLevedura.BioethanolCellobiohydrolaseCelluloseGenetic engineeringYeast.BioetanolCelobiohidrolasaCelulosaIngeniería genéticaLevadura.Biotechnology has been an essential tool in the search for solutions and in the optimization of bioprocesses associated with issues of human, plant, animal, energy and also the balance of ecosystems on planet Earth. The objective of this research was to present an unconventional substrate (cellulose), in abundance on the planet, to be used as a substitute source of carbon and energy for biotechnology processes, with the possibility of increasing industrial production of biomass and energy. As basis for the research, an extensive literature review and quantitative and qualitative analyzes were carried out. Genetic Engineering techniques were used to enable the yeast Saccharomyces cerevisiae for partial cellulose degradation, through the use of genetic transformation methods to insert a plasmid carrying the cellobiohydrolase cDNA. It was found that the recombinant and biologically active cellobiohydrolase protein was expressed and excreted in haploid and diploid laboratory yeast strains. The analyzes allowed the visualization of cellulolysis halos around colonies of recombinant strains grown in solid YPD medium with 1% microgranular cellulose. The recombinant clones derived from the haploid lineage yielded in average of 1.70 mg ART/mL, while recombinant clones derived from the diploid lineage produced in average of 2.05 mg ART/mL.La biotecnología ha sido una herramienta fundamental en la búsqueda de soluciones y en la optimización de bioprocesos relacionados con cuestiones humanas, vegetales, animales y energéticas, así como con el propio equilibrio de los ecosistemas de nuestro planeta. El propósito de esta investigación fue precisamente utilizar un sustrato no convencional y abundante en el planeta, la celulosa, para ser utilizada como fuente alternativa de carbono y energía a los procesos biotecnológicos, con posibilidad de incrementarse en la producción industrial de biomasa y energía. Como base para la investigación, se realizó una extensa revisión bibliográfica y análisis cuantitativos y cualitativos. Se utilizaron técnicas de ingeniería genética para permitir que la levadura Saccharomyces cerevisiae degradara parcialmente la celulosa, utilizando el método de transformación genética por electroporación para insertar un plásmido portador del cDNA de celobiohidrolasa. Se verificó que la proteína celobiohidrolasa recombinante y biológicamente activa se expresó y excretó en cepas de levadura de laboratorio haploides y diploides Saccharomyces cerevisiae que recibieron el plásmido con la introducción del inserto de CDNA - celobiohidrolasa. Los análisis permitieron visualizar halos de celulólisis alrededor de colonias de las cepas recombinantes cultivadas en medio sólido YPD con 1% de celulosa microgranular. Los clones recombinantes derivados del linaje haploide produjeron un promedio de 1,7 mg ART/mL; mientras que los clones recombinantes derivados del linaje diploide produjeron un promedio de 2.05 mg de ART/mL.A biotecnologia tem sido uma ferramenta fundamental na busca de soluções e na otimização de bioprocessos relacionados às questões de saúde humana, vegetal, animal, energia e também ao próprio equilíbrio de ecossistemas do nosso planeta. O propósito desta pesquisa foi justamente usar um substrato não convencional em abundância no planeta, a celulose, para ser usado como fonte de carbono e energia alternativos aos processos biotecnológicos, com a possibilidade de ser incrementado na produção industrial de biomassa e energia. Como base da pesquisa, realizou-se uma revisão bibliográfica extensa e análises do tipo quanti e qualitativas.  Foram utilizadas técnias da Engenharia Genética para capacitar a levedura Saccharomyces cerevisiae à degradação parcial da celulose, utilizando o método de transformação genética por eletroporação para inserir um plasmídeo carreador do cDNA da celobiohidrolase. Verificou-se que a proteína celobiohidrolase recombinante e biologicamente ativa foi expressada e excretada em linhagens da levedura Saccharomyces cerevisiae de laboratório haplóide e diplóide que receberam o plasmídeo com a introdução do inserto cDNA - celobiohidrolase. As análises permitiram a visualização de halos de celulolise ao redor de colônias das linhagens recombinantes crescidas em meio sólido YPD com 1% de celulose microgranular. Os clones recombinantes derivados da linhagem haplóide produziram na média1,7 mg de ART/mL; enquanto que os clones recombinantes derivados da linhagem diplóide produziram na média 2,05 mg de ART/mL.Research, Society and Development2022-12-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2790410.33448/rsd-v11i16.27904Research, Society and Development; Vol. 11 No. 16; e536111627904Research, Society and Development; Vol. 11 Núm. 16; e536111627904Research, Society and Development; v. 11 n. 16; e5361116279042525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/27904/31923Copyright (c) 2022 Jefferson Alves da Costa Junior; Elisabete José Vicente; Elza Teresinha Grael Marasca ; Fernando Araripe Gonçalves Torres ; Lidia Maria Pepe de Moraes ; Ubiratan Alegransi Bones https://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCosta Junior, Jefferson Alves da Vicente, Elisabete José Marasca , Elza Teresinha Grael Torres , Fernando Araripe Gonçalves Moraes , Lidia Maria Pepe de Bones , Ubiratan Alegransi 2022-12-18T18:26:42Zoai:ojs.pkp.sfu.ca:article/27904Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:45:29.268517Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Biotechnology: use of available carbon sources on the planet to generate alternatives energy
Biotecnología: uso de las fuentes de carbono disponibles en el planeta para generar energías alternativas
Biotecnologia: uso de fontes de carbono disponíveis no planeta para geração de energia alternativa
title Biotechnology: use of available carbon sources on the planet to generate alternatives energy
spellingShingle Biotechnology: use of available carbon sources on the planet to generate alternatives energy
Costa Junior, Jefferson Alves da
Bioetanol
Celobiohidrolase
Celulose
Engenharia genética
Levedura.
Bioethanol
Cellobiohydrolase
Cellulose
Genetic engineering
Yeast.
Bioetanol
Celobiohidrolasa
Celulosa
Ingeniería genética
Levadura.
title_short Biotechnology: use of available carbon sources on the planet to generate alternatives energy
title_full Biotechnology: use of available carbon sources on the planet to generate alternatives energy
title_fullStr Biotechnology: use of available carbon sources on the planet to generate alternatives energy
title_full_unstemmed Biotechnology: use of available carbon sources on the planet to generate alternatives energy
title_sort Biotechnology: use of available carbon sources on the planet to generate alternatives energy
author Costa Junior, Jefferson Alves da
author_facet Costa Junior, Jefferson Alves da
Vicente, Elisabete José
Marasca , Elza Teresinha Grael
Torres , Fernando Araripe Gonçalves
Moraes , Lidia Maria Pepe de
Bones , Ubiratan Alegransi
author_role author
author2 Vicente, Elisabete José
Marasca , Elza Teresinha Grael
Torres , Fernando Araripe Gonçalves
Moraes , Lidia Maria Pepe de
Bones , Ubiratan Alegransi
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Costa Junior, Jefferson Alves da
Vicente, Elisabete José
Marasca , Elza Teresinha Grael
Torres , Fernando Araripe Gonçalves
Moraes , Lidia Maria Pepe de
Bones , Ubiratan Alegransi
dc.subject.por.fl_str_mv Bioetanol
Celobiohidrolase
Celulose
Engenharia genética
Levedura.
Bioethanol
Cellobiohydrolase
Cellulose
Genetic engineering
Yeast.
Bioetanol
Celobiohidrolasa
Celulosa
Ingeniería genética
Levadura.
topic Bioetanol
Celobiohidrolase
Celulose
Engenharia genética
Levedura.
Bioethanol
Cellobiohydrolase
Cellulose
Genetic engineering
Yeast.
Bioetanol
Celobiohidrolasa
Celulosa
Ingeniería genética
Levadura.
description Biotechnology has been an essential tool in the search for solutions and in the optimization of bioprocesses associated with issues of human, plant, animal, energy and also the balance of ecosystems on planet Earth. The objective of this research was to present an unconventional substrate (cellulose), in abundance on the planet, to be used as a substitute source of carbon and energy for biotechnology processes, with the possibility of increasing industrial production of biomass and energy. As basis for the research, an extensive literature review and quantitative and qualitative analyzes were carried out. Genetic Engineering techniques were used to enable the yeast Saccharomyces cerevisiae for partial cellulose degradation, through the use of genetic transformation methods to insert a plasmid carrying the cellobiohydrolase cDNA. It was found that the recombinant and biologically active cellobiohydrolase protein was expressed and excreted in haploid and diploid laboratory yeast strains. The analyzes allowed the visualization of cellulolysis halos around colonies of recombinant strains grown in solid YPD medium with 1% microgranular cellulose. The recombinant clones derived from the haploid lineage yielded in average of 1.70 mg ART/mL, while recombinant clones derived from the diploid lineage produced in average of 2.05 mg ART/mL.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-17
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/27904
10.33448/rsd-v11i16.27904
url https://rsdjournal.org/index.php/rsd/article/view/27904
identifier_str_mv 10.33448/rsd-v11i16.27904
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/27904/31923
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 11 No. 16; e536111627904
Research, Society and Development; Vol. 11 Núm. 16; e536111627904
Research, Society and Development; v. 11 n. 16; e536111627904
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052794002210816

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