Polymorphisms in the Leptin and SCD genes in Buffaloes of the Amazon

Detalhes bibliográficos
Autor(a) principal: Silva, Caio Santos
Data de Publicação: 2021
Outros Autores: Silva Filho, Ednaldo da, Silva, Lorena Keyse Nery da, Ferraz, Rafaella Sousa, Matos, Amanda de Sousa, Marques, Larissa Coelho, Sales, Rodrigo Lima, Guimarães, Diva Anelie de Araújo, Marques, José Ribamar Felipe
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/21222
Resumo: The objective this work was to evaluate genetically the buffaloes populations using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in the leptin and stearoyl-CoA desaturase (SCD) genes, and associate the genotypes with milk production. Sixty-nine samples of Bubalus bubalis - 38 Murrah breed, 18 Mediterranean and 13 mixed-breed of the Murrah with Mediterranean - were evaluated. Allele and genotype frequencies, the heterozygosity observed and expected, the inbreeding coefficients (FIS), the probabilities of the Hardy-Weinberg equilibrium, the F statistic for population differentiation and Shannon index were calculated using GENEPOP and GenALEx programs. The associations of the different genotypes with a role in milk production were tested by Analysis of Variance (ANOVA) and t-test. We reported the leptin and SCD allele contribution to genetic variability in buffaloes herds in the Brazilian Amazonian region. The A allele was more representative in leptin and SCD genes for all breed groups. No significant effects between genotypes and milk production were found in the present study, but there is an indicative that AA genotype in leptin gene affects milk production in Murrah breed.
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spelling Polymorphisms in the Leptin and SCD genes in Buffaloes of the AmazonPolimorfismos en los genes de Leptina y SCD en Búfalas del AmazonasPolimorfismos nos genes Leptina e SCD em Búfalas da AmazôniaBubalus bubalisGenótipoLeiteSNP.Bubalus bubalisGenotipoLecheSNP.Bubalus bubalisGenotypeMilkSNP.The objective this work was to evaluate genetically the buffaloes populations using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in the leptin and stearoyl-CoA desaturase (SCD) genes, and associate the genotypes with milk production. Sixty-nine samples of Bubalus bubalis - 38 Murrah breed, 18 Mediterranean and 13 mixed-breed of the Murrah with Mediterranean - were evaluated. Allele and genotype frequencies, the heterozygosity observed and expected, the inbreeding coefficients (FIS), the probabilities of the Hardy-Weinberg equilibrium, the F statistic for population differentiation and Shannon index were calculated using GENEPOP and GenALEx programs. The associations of the different genotypes with a role in milk production were tested by Analysis of Variance (ANOVA) and t-test. We reported the leptin and SCD allele contribution to genetic variability in buffaloes herds in the Brazilian Amazonian region. The A allele was more representative in leptin and SCD genes for all breed groups. No significant effects between genotypes and milk production were found in the present study, but there is an indicative that AA genotype in leptin gene affects milk production in Murrah breed.Resumen - El objetivo de este trabajo fue evaluar genéticamente las poblaciones de búfalos mediante la reacción en cadena de polimerasa-polimorfismos de longitud de fragmentos de restricción (PCR-RFLP) en los genes de leptina y estearoil-CoA desaturasa (SCD), y asociar los genotipos con la producción de leche. Se evaluaron 69 muestras de Bubalus bubalis, 38 de la raza Murrah, 18 de la raza mediterránea y 13 mestizas de la raza Murrah con la mediterránea. Las frecuencias de alelos y genotipos, la heterocigosidad observada y esperada, los coeficientes de consanguinidad (FIS), las probabilidades del equilibrio de Hardy-Weinberg, el estadístico F para la diferenciación poblacional y el índice de Shannon se calcularon utilizando los programas GENEPOP y GenALEx. Las asociaciones de los diferentes genotipos con un papel en la producción de leche se probaron mediante Análisis de Varianza (ANOVA) y prueba t . Reportamos la contribución de la leptina y el alelo SCD a la variabilidad genética en rebaños de búfalos en la región amazónica brasileña. El alelo A fue más representativo en los genes de leptina y SCD para todos los grupos de razas. En el presente estudio no se encontraron efectos significativos entre los genotipos y la producción de leche, pero hay indicios de que el genotipo AA en el gen de la leptina afecta la producción de leche en la raza Murrah.O objetivo deste trabalho foi avaliar geneticamente as populações de búfalas por meio da reação em cadeia da polimerase e do polimorfismo no comprimento do fragmento de restrição (PCR-RFLP) nos genes da leptina e estearoil-CoA dessaturase (SCD), e associar os genótipos à produção de leite. Foram avaliadas 69 amostras de Bubalus bubalis - 38 da raça Murrah, 18 mediterrâneo e 13 mestiças da raça Murrah com Mediterrâneo. As frequências de alelos e genótipos, a heterozigosidade observada e esperada, os coeficientes de endogamia (FIS), as probabilidades de equilíbrio de Hardy-Weinberg, a estatística F para diferenciação populacional e o índice de Shannon foram calculados usando os programas GENEPOP e GenALEx. As associações dos diferentes genótipos com o papel na produção de leite foram testadas por Análise de Variância (ANOVA) e teste t. Nós relatamos a contribuição da leptina e do alelo SCD para a variabilidade genética em rebanhos bubalinos na região amazônica brasileira. O alelo A foi o mais representativo nos genes da leptina e SCD para todos os grupos raciais. Nenhum efeito significativo entre os genótipos e a produção de leite foi encontrado no presente estudo, mas há um indicativo de que o genótipo AA no gene da leptina afeta a produção de leite na raça Murrah.Research, Society and Development2021-10-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2122210.33448/rsd-v10i13.21222Research, Society and Development; Vol. 10 No. 13; e233101321222Research, Society and Development; Vol. 10 Núm. 13; e233101321222Research, Society and Development; v. 10 n. 13; e2331013212222525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/21222/18857Copyright (c) 2021 Caio Santos Silva; Ednaldo da Silva Filho; Lorena Keyse Nery da Silva; Rafaella Sousa Ferraz; Amanda de Sousa Matos; Larissa Coelho Marques; Rodrigo Lima Sales; Diva Anelie de Araújo Guimarães; José Ribamar Felipe Marqueshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccess Silva, Caio SantosSilva Filho, Ednaldo daSilva, Lorena Keyse Nery daFerraz, Rafaella SousaMatos, Amanda de SousaMarques, Larissa Coelho Sales, Rodrigo Lima Guimarães, Diva Anelie de AraújoMarques, José Ribamar Felipe 2021-11-21T18:26:28Zoai:ojs.pkp.sfu.ca:article/21222Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:40:41.400503Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Polymorphisms in the Leptin and SCD genes in Buffaloes of the Amazon
Polimorfismos en los genes de Leptina y SCD en Búfalas del Amazonas
Polimorfismos nos genes Leptina e SCD em Búfalas da Amazônia
title Polymorphisms in the Leptin and SCD genes in Buffaloes of the Amazon
spellingShingle Polymorphisms in the Leptin and SCD genes in Buffaloes of the Amazon
Silva, Caio Santos
Bubalus bubalis
Genótipo
Leite
SNP.
Bubalus bubalis
Genotipo
Leche
SNP.
Bubalus bubalis
Genotype
Milk
SNP.
title_short Polymorphisms in the Leptin and SCD genes in Buffaloes of the Amazon
title_full Polymorphisms in the Leptin and SCD genes in Buffaloes of the Amazon
title_fullStr Polymorphisms in the Leptin and SCD genes in Buffaloes of the Amazon
title_full_unstemmed Polymorphisms in the Leptin and SCD genes in Buffaloes of the Amazon
title_sort Polymorphisms in the Leptin and SCD genes in Buffaloes of the Amazon
author Silva, Caio Santos
author_facet Silva, Caio Santos
Silva Filho, Ednaldo da
Silva, Lorena Keyse Nery da
Ferraz, Rafaella Sousa
Matos, Amanda de Sousa
Marques, Larissa Coelho
Sales, Rodrigo Lima
Guimarães, Diva Anelie de Araújo
Marques, José Ribamar Felipe
author_role author
author2 Silva Filho, Ednaldo da
Silva, Lorena Keyse Nery da
Ferraz, Rafaella Sousa
Matos, Amanda de Sousa
Marques, Larissa Coelho
Sales, Rodrigo Lima
Guimarães, Diva Anelie de Araújo
Marques, José Ribamar Felipe
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Caio Santos
Silva Filho, Ednaldo da
Silva, Lorena Keyse Nery da
Ferraz, Rafaella Sousa
Matos, Amanda de Sousa
Marques, Larissa Coelho
Sales, Rodrigo Lima
Guimarães, Diva Anelie de Araújo
Marques, José Ribamar Felipe
dc.subject.por.fl_str_mv Bubalus bubalis
Genótipo
Leite
SNP.
Bubalus bubalis
Genotipo
Leche
SNP.
Bubalus bubalis
Genotype
Milk
SNP.
topic Bubalus bubalis
Genótipo
Leite
SNP.
Bubalus bubalis
Genotipo
Leche
SNP.
Bubalus bubalis
Genotype
Milk
SNP.
description The objective this work was to evaluate genetically the buffaloes populations using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in the leptin and stearoyl-CoA desaturase (SCD) genes, and associate the genotypes with milk production. Sixty-nine samples of Bubalus bubalis - 38 Murrah breed, 18 Mediterranean and 13 mixed-breed of the Murrah with Mediterranean - were evaluated. Allele and genotype frequencies, the heterozygosity observed and expected, the inbreeding coefficients (FIS), the probabilities of the Hardy-Weinberg equilibrium, the F statistic for population differentiation and Shannon index were calculated using GENEPOP and GenALEx programs. The associations of the different genotypes with a role in milk production were tested by Analysis of Variance (ANOVA) and t-test. We reported the leptin and SCD allele contribution to genetic variability in buffaloes herds in the Brazilian Amazonian region. The A allele was more representative in leptin and SCD genes for all breed groups. No significant effects between genotypes and milk production were found in the present study, but there is an indicative that AA genotype in leptin gene affects milk production in Murrah breed.
publishDate 2021
dc.date.none.fl_str_mv 2021-10-10
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/21222
10.33448/rsd-v10i13.21222
url https://rsdjournal.org/index.php/rsd/article/view/21222
identifier_str_mv 10.33448/rsd-v10i13.21222
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/21222/18857
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 13; e233101321222
Research, Society and Development; Vol. 10 Núm. 13; e233101321222
Research, Society and Development; v. 10 n. 13; e233101321222
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052819308544000

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