Polymorphisms in osteopontin gene in Amazon buffaloes

• Autor(a) principal: Rolim Filho, Sebastião Tavares
• Data de Publicação: 2013
• Outros Autores: Ribeiro, Haroldo Francisco Lobato, Camargo, Gregório Miguel Ferreira de [UNESP], Cardoso, Diercles Francisco [UNESP], Aspilcueta-Borquis, Raul Rusbel [UNESP], Tonhati, Humberto [UNESP], Nunes, Kim de Borborema, Vale, William Gomes, Sousa, Keitiane Colares de, Mesquita, Ellen Yasmin Eguchi, Boaretto, José Luiz, Alcântara, Gisélia de Lourdes Cardoso de
• Tipo de documento: Artigo de conferência
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/232265
• topic: Bubalus bubalis; Reproductive traits; SNP

The aim of this work was to identify polymorphisms in the osteopontin gene. It was used in this experiment 306 male buffaloes, older than 18 months, bred in two farms, one in the State of Amapá and the other farm in the State of Pará. There was identified three SNP polymorphisms for the region amplified by the primer OS4 (5'upstream) and four SNP polymorphisms for the region amplified by the primer OS9 (exon 5 to exon 6). The polymorphisms were in positions 1478, 1513 and 1611 in the region amplified by OS4 and positions 6690, 6737, 6925 and 6952 in the region amplified by OS9. These data indicate that the osteopontin gene is important because it can have a substantial influence on the reproductive traits of male buffaloes.