In vitro antagonistic activity of Sclerotium sp. by Bacillus sp.

Detalhes bibliográficos
Autor(a) principal: Zambrano, Melissa Alezandra Romero
Data de Publicação: 2022
Outros Autores: Castanharo, Gianny Roger Parra, Aguiar, Raimundo Wagner de Souza, Chagas Junior, Aloisio Freitas
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/35351
Resumo: Bacteria from the genus Bacillus sp. have a high potential for the biological control of phytopathogenic fungi. Therefore, in the present research we proposed to evaluate the antagonistic capacity of Bacillus sp. isolates against the phytopathogenic fungus Sclerotium sp. The tests were performed using two methods (dual and modified dual), also using five Bacillus sp. isolates (LBM01, LBM02, LBM03, LBM04 and LBM06). Mycelial growth evaluations were performed on BDA culture medium. All experiments were performed in triplicate and the plates were incubated under a 12 h photoperiod at 28±1 ºC for 7 days. Radial growth of the pathogens was measured at the intervals of 24, 48, 72, 96, 120, 144 and 168 h. The percentage of inhibition was calculated using the equation: I% = [(C-T)/C]x100. All Bacillus sp. isolates tested were able to inhibit the mycelial growth of Sclerotium sp. within 72 h, while isolates LBM01, LBM03 and LBM06 were able to maintain inhibition for 168 h. The isolate LBM06 showed the highest percentage of inhibition in all two culture methods used.
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spelling In vitro antagonistic activity of Sclerotium sp. by Bacillus sp.Actividad antagónica in vitro de Sclerotium sp. por Bacillus sp.Atividade antagônica in vitro de Sclerotium sp. por Bacillus sp.BiocontrolPhytopathogenic fungusRhizosphere bacteria.BiocontrolHongo fitopatógenoBacterias de la rizosfera.BiocontroleFungo fitopatogênicoBactéria da rizosfera.Bacteria from the genus Bacillus sp. have a high potential for the biological control of phytopathogenic fungi. Therefore, in the present research we proposed to evaluate the antagonistic capacity of Bacillus sp. isolates against the phytopathogenic fungus Sclerotium sp. The tests were performed using two methods (dual and modified dual), also using five Bacillus sp. isolates (LBM01, LBM02, LBM03, LBM04 and LBM06). Mycelial growth evaluations were performed on BDA culture medium. All experiments were performed in triplicate and the plates were incubated under a 12 h photoperiod at 28±1 ºC for 7 days. Radial growth of the pathogens was measured at the intervals of 24, 48, 72, 96, 120, 144 and 168 h. The percentage of inhibition was calculated using the equation: I% = [(C-T)/C]x100. All Bacillus sp. isolates tested were able to inhibit the mycelial growth of Sclerotium sp. within 72 h, while isolates LBM01, LBM03 and LBM06 were able to maintain inhibition for 168 h. The isolate LBM06 showed the highest percentage of inhibition in all two culture methods used.Las bacterias pertenecientes al género Bacillus sp. tienen un alto potencial para el control biológico de hongos fitopatógenos. Por lo tanto, en la presente investigación nos propusimos evaluar la capacidad antagónica de los aislados de Bacillus sp. contra el hongo fitopatógeno Sclerotium sp. Las pruebas se realizaron utilizando dos métodos (dual y dual modificado), así como cinco aislados de Bacillus sp. (LBM01, LBM02, LBM03, LBM04 y LBM06). Las evaluaciones del crecimiento micelial se realizaron en medio de cultivo BDA. Todos los experimentos se realizaron por triplicado y las placas se incubaron bajo un fotoperiodo de 12 h a 28±1 ºC durante 7 días. El crecimiento radial de los patógenos se midió a intervalos de 24, 48, 72, 96, 120, 144 y 168 horas. El porcentaje de inhibición se calculó mediante la ecuación: I% = [(C-T)/C]x100. Todos los aislados de Bacillus sp. probados fueron capaces de inhibir el crecimiento micelial de Sclerotium sp. en 72 h, mientras que los aislados LBM01, LBM03 y LBM06 fueron capaces de mantener la inhibición durante 168 h. El aislado LBM06 mostró el mayor porcentaje de inhibición en todos los dos métodos de cultivo utilizados.As bactérias pertencentes ao gênero Bacillus sp. têm alto potencial para o controle biológico de fungos fitopatogênicos. Portanto, na presente pesquisa nos propusemos avaliar a capacidade antagônica de isolados de Bacillus sp. contra o fungo fitopatogênico Sclerotium sp. Os testes foram realizados utilizando dois métodos (dual e dual modificado), assim como cinco isolados de Bacillus sp. (LBM01, LBM02, LBM03, LBM04 e LBM06). As avaliações do crescimento micelial foram realizadas no meio de cultura BDA. Todos os experimentos foram realizados em triplicata e as placas foram incubadas sob fotoperíodo de 12 h a 28±1 ºC durante 7 dias. O crescimento radial dos patógenos foi medido nos intervalos de 24, 48, 72, 96, 120, 144 e 168 horas. A porcentagem de inibição foi calculada através da equação: I% = [(C-T)/C]x100. Todos os isolados de Bacillus sp. testados foram capazes de inibir o crescimento micelial do Sclerotium sp. em até 72 h, enquanto os isolados LBM01, LBM03 e LBM06 foram capazes de manter a inibição durante 168 h. O isolado LBM06 revelou a maior porcentagem de inibição em todos os dois métodos de cultura utilizados.Research, Society and Development2022-10-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/3535110.33448/rsd-v11i13.35351Research, Society and Development; Vol. 11 No. 13; e380111335351Research, Society and Development; Vol. 11 Núm. 13; e380111335351Research, Society and Development; v. 11 n. 13; e3801113353512525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/35351/29821Copyright (c) 2022 Melissa Alezandra Romero Zambrano; Gianny Roger Parra Castanharo; Raimundo Wagner de Souza Aguiar; Aloisio Freitas Chagas Juniorhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessZambrano, Melissa Alezandra Romero Castanharo, Gianny Roger Parra Aguiar, Raimundo Wagner de Souza Chagas Junior, Aloisio Freitas2022-10-17T13:43:46Zoai:ojs.pkp.sfu.ca:article/35351Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:50:18.068476Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv In vitro antagonistic activity of Sclerotium sp. by Bacillus sp.
Actividad antagónica in vitro de Sclerotium sp. por Bacillus sp.
Atividade antagônica in vitro de Sclerotium sp. por Bacillus sp.
title In vitro antagonistic activity of Sclerotium sp. by Bacillus sp.
spellingShingle In vitro antagonistic activity of Sclerotium sp. by Bacillus sp.
Zambrano, Melissa Alezandra Romero
Biocontrol
Phytopathogenic fungus
Rhizosphere bacteria.
Biocontrol
Hongo fitopatógeno
Bacterias de la rizosfera.
Biocontrole
Fungo fitopatogênico
Bactéria da rizosfera.
title_short In vitro antagonistic activity of Sclerotium sp. by Bacillus sp.
title_full In vitro antagonistic activity of Sclerotium sp. by Bacillus sp.
title_fullStr In vitro antagonistic activity of Sclerotium sp. by Bacillus sp.
title_full_unstemmed In vitro antagonistic activity of Sclerotium sp. by Bacillus sp.
title_sort In vitro antagonistic activity of Sclerotium sp. by Bacillus sp.
author Zambrano, Melissa Alezandra Romero
author_facet Zambrano, Melissa Alezandra Romero
Castanharo, Gianny Roger Parra
Aguiar, Raimundo Wagner de Souza
Chagas Junior, Aloisio Freitas
author_role author
author2 Castanharo, Gianny Roger Parra
Aguiar, Raimundo Wagner de Souza
Chagas Junior, Aloisio Freitas
author2_role author
author
author
dc.contributor.author.fl_str_mv Zambrano, Melissa Alezandra Romero
Castanharo, Gianny Roger Parra
Aguiar, Raimundo Wagner de Souza
Chagas Junior, Aloisio Freitas
dc.subject.por.fl_str_mv Biocontrol
Phytopathogenic fungus
Rhizosphere bacteria.
Biocontrol
Hongo fitopatógeno
Bacterias de la rizosfera.
Biocontrole
Fungo fitopatogênico
Bactéria da rizosfera.
topic Biocontrol
Phytopathogenic fungus
Rhizosphere bacteria.
Biocontrol
Hongo fitopatógeno
Bacterias de la rizosfera.
Biocontrole
Fungo fitopatogênico
Bactéria da rizosfera.
description Bacteria from the genus Bacillus sp. have a high potential for the biological control of phytopathogenic fungi. Therefore, in the present research we proposed to evaluate the antagonistic capacity of Bacillus sp. isolates against the phytopathogenic fungus Sclerotium sp. The tests were performed using two methods (dual and modified dual), also using five Bacillus sp. isolates (LBM01, LBM02, LBM03, LBM04 and LBM06). Mycelial growth evaluations were performed on BDA culture medium. All experiments were performed in triplicate and the plates were incubated under a 12 h photoperiod at 28±1 ºC for 7 days. Radial growth of the pathogens was measured at the intervals of 24, 48, 72, 96, 120, 144 and 168 h. The percentage of inhibition was calculated using the equation: I% = [(C-T)/C]x100. All Bacillus sp. isolates tested were able to inhibit the mycelial growth of Sclerotium sp. within 72 h, while isolates LBM01, LBM03 and LBM06 were able to maintain inhibition for 168 h. The isolate LBM06 showed the highest percentage of inhibition in all two culture methods used.
publishDate 2022
dc.date.none.fl_str_mv 2022-10-10
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/35351
10.33448/rsd-v11i13.35351
url https://rsdjournal.org/index.php/rsd/article/view/35351
identifier_str_mv 10.33448/rsd-v11i13.35351
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/35351/29821
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 11 No. 13; e380111335351
Research, Society and Development; Vol. 11 Núm. 13; e380111335351
Research, Society and Development; v. 11 n. 13; e380111335351
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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