HPLC analysis of β-caryophyllene in copaiba oleoresin: Development, validation and applicability

Detalhes bibliográficos
Autor(a) principal: Oliveira, Mariana Carla de
Data de Publicação: 2022
Outros Autores: Dano, Maria Eduarda Lima, Santos, Rafaela Said dos, Villa Nova, Mônica, Nakamura, Celso Vataru, Caetano, Wilker, Bruschi, Marcos Luciano
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/32525
Resumo: Copaiba oil (CO) is an oleoresin containing resinous acids, comprising mainly of diterpenes, and volatile compounds, comprising of sesquiterpenes. CO has been used for many years as a therapeutic agent and cosmetic, being the β-caryophyllene (CAR) one of the main sesquiterpene markers found in CO samples. During the last years, some analytical methods have been developed for analysis of sesquiterpenes like CAR from CO. However, these methods are based on gas chromatography, and requiring additional steps, such as derivatization or extraction of the essential fraction of the CO for sesquiterpenes analysis. Liquid chromatography methodologies have been proposed only for analysis of diterpenes. Therefore, the aim of this study was to develop a high-performance liquid chromatography (HPLC) assay for CAR analysis in CO samples (Copaifera reticulata Ducke) and in emulsion systems containing CO. The HPLC system suitability was determined through the capacity factor, repeatability, relative retention, resolution, tailing factor, theoretical plate number and the height of the theoretical plate; where the method developed showed the efficiency for separation of CO compounds. The method was validated displaying specificity, linearity, precision, accuracy, and robustness. Moreover, it showed to be of utmost importance to analyze CO in emulsion systems, displaying versatile and applicability.
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spelling HPLC analysis of β-caryophyllene in copaiba oleoresin: Development, validation and applicabilityAnálisis por HPLC de β-cariofileno en aceite-resina de copaiba: Desarrollo, validación y aplicabilidadAnálise por CLAE de β-cariofileno em óleo-resina de copaíba: Desenvolvimento, validação e aplicabilidadeCopaifera sppBeta-cariofilenoHPLCEmulsiónAnálisis de sesquiterpenos.Copaifera sppBeta-cariofilenoCLAEEmulsãoAnálise de sesquiterpeno.Copaifera sppBeta-caryophylleneHPLCEmulsionSesquiterpenes analysis.Copaiba oil (CO) is an oleoresin containing resinous acids, comprising mainly of diterpenes, and volatile compounds, comprising of sesquiterpenes. CO has been used for many years as a therapeutic agent and cosmetic, being the β-caryophyllene (CAR) one of the main sesquiterpene markers found in CO samples. During the last years, some analytical methods have been developed for analysis of sesquiterpenes like CAR from CO. However, these methods are based on gas chromatography, and requiring additional steps, such as derivatization or extraction of the essential fraction of the CO for sesquiterpenes analysis. Liquid chromatography methodologies have been proposed only for analysis of diterpenes. Therefore, the aim of this study was to develop a high-performance liquid chromatography (HPLC) assay for CAR analysis in CO samples (Copaifera reticulata Ducke) and in emulsion systems containing CO. The HPLC system suitability was determined through the capacity factor, repeatability, relative retention, resolution, tailing factor, theoretical plate number and the height of the theoretical plate; where the method developed showed the efficiency for separation of CO compounds. The method was validated displaying specificity, linearity, precision, accuracy, and robustness. Moreover, it showed to be of utmost importance to analyze CO in emulsion systems, displaying versatile and applicability.El aceite de copaiba (CO) es una oleorresina que contiene ácidos resinosos, compuestos principalmente por diterpenos, y compuestos volátiles, compuestos por sesquiterpenos. El CO se ha utilizado durante muchos años como agente terapéutico y cosmético, siendo el β-cariofileno (CAR) uno de los principales marcadores de sesquiterpenos encontrados en muestras de CO. Durante los últimos años se han desarrollado algunos métodos analíticos para el análisis de sesquiterpenos como CAR a partir de CO. Sin embargo, estos métodos se basan en la cromatografía de gases y requieren pasos adicionales, como la derivatización o extracción de la fracción esencial del CO para el análisis de sesquiterpenos. Las metodologías de cromatografía líquida se han propuesto solo para el análisis de diterpenos. Por lo tanto, el objetivo de este estudio fue desarrollar un ensayo de cromatografía líquida de alta resolución (HPLC) para el análisis de CAR en muestras de CO (Copaifera reticulata Ducke) y en sistemas emulsionantes que contienen CO. La idoneidad del sistema de HPLC se determinó mediante el factor de capacidad, la repetibilidad, retención relativa, resolución, factor de simetría, número de plato teórico y altura del plato teórico; donde el método desarrollado mostró la eficiencia para la separación de compuestos de CO. El método fue validado mostrando especificidad, linealidad, precisión, exactitud y robustez. Además, demostró ser de suma importancia analizar CO en sistemas emulsionantes, mostrando versatilidad y aplicabilidad.O óleo de copaíba (CO) é um óleo-resina contendo ácidos resinosos, compostos principalmente por diterpenos, e compostos voláteis, constituído por sesquiterpenos. O CO é utilizado há muitos anos como agente terapêutico e cosmético, sendo o β-cariofileno (CAR) um dos principais marcadores sesquiterpênicos. Nos últimos anos, alguns métodos analíticos foram desenvolvidos para análise de sesquiterpenos como o CAR a partir de amostras de CO. No entanto, esses métodos são baseados em cromatografia gasosa e requerem etapas adicionais, como derivatização ou extração da fração volátil do CO para análise dos sesquiterpenos. Metodologias de cromatografia líquida têm sido propostas apenas para análise dos diterpenos. Portanto, o objetivo deste estudo foi desenvolver um método de cromatografia líquida de alta eficiência (HPLC) para análise de CAR em amostras de CO (Copaifera reticulata Ducke) e em sistemas emulsivos contendo CO. A adequação do sistema de HPLC foi determinada através do fator de capacidade, repetibilidade, fator de separação, resolução, fator de simetria, número de pratos teóricos e altura dos pratos teóricos; onde o método desenvolvido mostrou eficiência para separação de compostos presentes no CO. O método foi validado apresentando especificidade, linearidade, precisão, exatidão e robustez. Além disso, mostrou-se de suma importância analisar o CO em sistemas emulsivos, apresentando versatilidade e aplicabilidade.Research, Society and Development2022-07-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/3252510.33448/rsd-v11i10.32525Research, Society and Development; Vol. 11 No. 10; e129111032525Research, Society and Development; Vol. 11 Núm. 10; e129111032525Research, Society and Development; v. 11 n. 10; e1291110325252525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/32525/27617Copyright (c) 2022 Mariana Carla de Oliveira; Maria Eduarda Lima Dano; Rafaela Said dos Santos; Mônica Villa Nova; Celso Vataru Nakamura; Wilker Caetano; Marcos Luciano Bruschihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessOliveira, Mariana Carla de Dano, Maria Eduarda LimaSantos, Rafaela Said dos Villa Nova, MônicaNakamura, Celso VataruCaetano, WilkerBruschi, Marcos Luciano2022-08-12T22:23:03Zoai:ojs.pkp.sfu.ca:article/32525Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:48:30.002149Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv HPLC analysis of β-caryophyllene in copaiba oleoresin: Development, validation and applicability
Análisis por HPLC de β-cariofileno en aceite-resina de copaiba: Desarrollo, validación y aplicabilidad
Análise por CLAE de β-cariofileno em óleo-resina de copaíba: Desenvolvimento, validação e aplicabilidade
title HPLC analysis of β-caryophyllene in copaiba oleoresin: Development, validation and applicability
spellingShingle HPLC analysis of β-caryophyllene in copaiba oleoresin: Development, validation and applicability
Oliveira, Mariana Carla de
Copaifera spp
Beta-cariofileno
HPLC
Emulsión
Análisis de sesquiterpenos.
Copaifera spp
Beta-cariofileno
CLAE
Emulsão
Análise de sesquiterpeno.
Copaifera spp
Beta-caryophyllene
HPLC
Emulsion
Sesquiterpenes analysis.
title_short HPLC analysis of β-caryophyllene in copaiba oleoresin: Development, validation and applicability
title_full HPLC analysis of β-caryophyllene in copaiba oleoresin: Development, validation and applicability
title_fullStr HPLC analysis of β-caryophyllene in copaiba oleoresin: Development, validation and applicability
title_full_unstemmed HPLC analysis of β-caryophyllene in copaiba oleoresin: Development, validation and applicability
title_sort HPLC analysis of β-caryophyllene in copaiba oleoresin: Development, validation and applicability
author Oliveira, Mariana Carla de
author_facet Oliveira, Mariana Carla de
Dano, Maria Eduarda Lima
Santos, Rafaela Said dos
Villa Nova, Mônica
Nakamura, Celso Vataru
Caetano, Wilker
Bruschi, Marcos Luciano
author_role author
author2 Dano, Maria Eduarda Lima
Santos, Rafaela Said dos
Villa Nova, Mônica
Nakamura, Celso Vataru
Caetano, Wilker
Bruschi, Marcos Luciano
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Mariana Carla de
Dano, Maria Eduarda Lima
Santos, Rafaela Said dos
Villa Nova, Mônica
Nakamura, Celso Vataru
Caetano, Wilker
Bruschi, Marcos Luciano
dc.subject.por.fl_str_mv Copaifera spp
Beta-cariofileno
HPLC
Emulsión
Análisis de sesquiterpenos.
Copaifera spp
Beta-cariofileno
CLAE
Emulsão
Análise de sesquiterpeno.
Copaifera spp
Beta-caryophyllene
HPLC
Emulsion
Sesquiterpenes analysis.
topic Copaifera spp
Beta-cariofileno
HPLC
Emulsión
Análisis de sesquiterpenos.
Copaifera spp
Beta-cariofileno
CLAE
Emulsão
Análise de sesquiterpeno.
Copaifera spp
Beta-caryophyllene
HPLC
Emulsion
Sesquiterpenes analysis.
description Copaiba oil (CO) is an oleoresin containing resinous acids, comprising mainly of diterpenes, and volatile compounds, comprising of sesquiterpenes. CO has been used for many years as a therapeutic agent and cosmetic, being the β-caryophyllene (CAR) one of the main sesquiterpene markers found in CO samples. During the last years, some analytical methods have been developed for analysis of sesquiterpenes like CAR from CO. However, these methods are based on gas chromatography, and requiring additional steps, such as derivatization or extraction of the essential fraction of the CO for sesquiterpenes analysis. Liquid chromatography methodologies have been proposed only for analysis of diterpenes. Therefore, the aim of this study was to develop a high-performance liquid chromatography (HPLC) assay for CAR analysis in CO samples (Copaifera reticulata Ducke) and in emulsion systems containing CO. The HPLC system suitability was determined through the capacity factor, repeatability, relative retention, resolution, tailing factor, theoretical plate number and the height of the theoretical plate; where the method developed showed the efficiency for separation of CO compounds. The method was validated displaying specificity, linearity, precision, accuracy, and robustness. Moreover, it showed to be of utmost importance to analyze CO in emulsion systems, displaying versatile and applicability.
publishDate 2022
dc.date.none.fl_str_mv 2022-07-25
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/32525
10.33448/rsd-v11i10.32525
url https://rsdjournal.org/index.php/rsd/article/view/32525
identifier_str_mv 10.33448/rsd-v11i10.32525
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/32525/27617
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 11 No. 10; e129111032525
Research, Society and Development; Vol. 11 Núm. 10; e129111032525
Research, Society and Development; v. 11 n. 10; e129111032525
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052718483767296

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