Microbiological analysis of phytoterapic symptoms (hedera helix l.) for adult/ pediatric use marketed in the city of Teresina-PI
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/24163 |
Resumo: | According to DRC No. 26/2014, herbal medicines are a product obtained from active vegetable raw material, with a prophylactic, curative or palliative purpose. The need for microbiological control of these products is important, mainly due to the safety, efficacy and acceptability of these products. The objective of this work was to evaluate the microbiological control of syrups based on the plant Hedera helix L. Two brands of herbal syrups were analyzed. Two dilutions (1:10 and 1: 100) were carried out with neutralization of the preservatives, and subsequent transfer to the enrichment broth, and incubation in the oven at 37 ° C for 24hrs. For the total counting of mesophilic microorganisms, the in-depth method was used for molds and yeasts using a surface method and for the detection of microorganisms, the presence of Escherichia coli, Staphylococcus aureus, Salmonella sp. In sample 1, there was growth of white and yellow colonies at the dilution 10¹, and for dilution at 10², there was no microbial growth. However, for sample 2 at the dilution of 10¹, there were countless small yellow colonies with Gram-positive cocci, while at the 10² dilution there was no growth. For the counting of molds and yeasts, in sample 1 in both dilutions, did not identify fungal form and sample 2 in dilution 10¹ found countless mucoid colonies, with brownish coloration. The present work demonstrated the need for microbiological control of herbal products since the safety, efficacy and acceptability of these products need to be maintained to avoid changes in their physical and chemical properties and to avoid the risk of infection for their users. |
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Microbiological analysis of phytoterapic symptoms (hedera helix l.) for adult/ pediatric use marketed in the city of Teresina-PIAnálisis microbiológico de jarabes de fitoterapia (hedera helix l.) para uso adulto/pediátrico vendidos en la ciudad de Teresina-PIAnálise microbiológica de xaropes fitoterápicos (hedera helix l.) de uso adulto/pediátrico comercializados na cidade de Teresina-PIMicrobiological ControlHerbal medicinesBacterium.Control MicrobiológicoHierbas medicinalesBacterias.Controle MicrobiológicoFitoterápicosBactérias.According to DRC No. 26/2014, herbal medicines are a product obtained from active vegetable raw material, with a prophylactic, curative or palliative purpose. The need for microbiological control of these products is important, mainly due to the safety, efficacy and acceptability of these products. The objective of this work was to evaluate the microbiological control of syrups based on the plant Hedera helix L. Two brands of herbal syrups were analyzed. Two dilutions (1:10 and 1: 100) were carried out with neutralization of the preservatives, and subsequent transfer to the enrichment broth, and incubation in the oven at 37 ° C for 24hrs. For the total counting of mesophilic microorganisms, the in-depth method was used for molds and yeasts using a surface method and for the detection of microorganisms, the presence of Escherichia coli, Staphylococcus aureus, Salmonella sp. In sample 1, there was growth of white and yellow colonies at the dilution 10¹, and for dilution at 10², there was no microbial growth. However, for sample 2 at the dilution of 10¹, there were countless small yellow colonies with Gram-positive cocci, while at the 10² dilution there was no growth. For the counting of molds and yeasts, in sample 1 in both dilutions, did not identify fungal form and sample 2 in dilution 10¹ found countless mucoid colonies, with brownish coloration. The present work demonstrated the need for microbiological control of herbal products since the safety, efficacy and acceptability of these products need to be maintained to avoid changes in their physical and chemical properties and to avoid the risk of infection for their users.Según RDC No. 26/2014, los productos fitoterapéuticos son un producto obtenido a partir de materia prima activa vegetal, con finalidad profiláctica, curativa o paliativa. La necesidad de control microbiológico de estos productos es importante, principalmente debido a la seguridad, eficacia y aceptabilidad de estos productos. El objetivo de este trabajo fue evaluar el control microbiológico de jarabes basados en la planta Hedera helix L. Se analizaron dos marcas de jarabes de hierbas. Se realizaron dos diluciones (1:10 y 1: 100) con neutralización de los conservantes y posterior transferencia al caldo de enriquecimiento e incubación en estufa a 37ºC durante 24 h. Para el recuento total de microorganismos mesófilos se utilizó el método en profundidad, para mohos y levaduras el método de superficie, y para la investigación de microorganismos, la presencia de las bacterias Escherichia coli, Staphylococcus aureus, Salmonella sp. En la muestra 1, se observó crecimiento de colonias blancas y amarillas en la dilución 10¹, y para la dilución 10², no hubo crecimiento microbiano. En cuanto a la muestra 2, en la dilución 10¹, se verificaron incontables pequeñas colonias amarillas, con confirmación de cocos Gram positivos, mientras que en la dilución 10 no hubo crecimiento. Para el recuento de mohos y levaduras, en la muestra 1 a ambas diluciones no se identificó forma fúngica y la muestra 2 en la dilución 10¹ encontró innumerables colonias mucoides, de color pardusco. El presente trabajo demostró la necesidad de un control microbiológico de los productos herbales, ya que es necesario mantener la seguridad, eficacia y aceptabilidad de estos productos para evitar cambios en sus propiedades físicas y químicas y evitar el riesgo de infección para sus usuarios.Segundo a RDC N° 26/2014, fitoterápicos são produto obtido de matéria-prima ativa vegetal, com finalidade profilática, curativa ou paliativa. A necessidade do controle microbiológico desses produtos é importante, devido, principalmente, à segurança, eficácia e aceitabilidade desses produtos. O trabalho teve como objetivo avaliar o controle microbiológico de xaropes a base da planta Hedera helix L. Foram analisadas duas marcas de xaropes fitoterápicos. Foram realizadas duas diluições (1:10 e 1:100) com neutralização dos conservantes, e posterior transferência para o caldo de enriquecimento, e incubação na estufa a 37ºC por 24hrs. Para a contagem total de microrganismos mesófilos foi utilizado o Método em profundidade, para bolores e leveduras utilizou método em superfície e para pesquisa de microrganismos, a presença das bactérias Escherichia coli, Staphylococcus aureus, Salmonella sp. Na amostra 1, observou crescimento de colônias brancas e amarelas na diluição 10¹, e para diluição em 10², não houve crescimento microbiano. Já para amostra 2 na diluição de 10¹ verificou incontáveis colônias amarelas pequenas, com constatação de cocos Gram positivos, enquanto que na diluição 10², não teve nenhum crescimento. Para a contagem de bolores e leveduras, na amostra 1 em ambas diluições, não identificou forma fúngica e amostra 2 na diluição 10¹ constatou incontáveis colônias mucoides, com coloração acastanhada. O presente trabalho, comprovou a necessidade do controle microbiológico de produtos fitoterápicos uma vez que a segurança, eficácia e aceitabilidade desses produtos precisam ser mantidas para evitar alterações em suas propriedades físicas e químicas e evitar risco de infecção para seus usuários.Research, Society and Development2022-08-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2416310.33448/rsd-v11i10.24163Research, Society and Development; Vol. 11 No. 10; e37111024163Research, Society and Development; Vol. 11 Núm. 10; e37111024163Research, Society and Development; v. 11 n. 10; e371110241632525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/24163/28127Copyright (c) 2022 Ana Maria Castro Ferreira; Leilane Ribeiro de Sousa; Mateus Sávio Amorim; Maria Jucileide Alves; Viviane De Sousa Santos; Joelma Cristina Silva de Jesus Leal; Nayana Mirian Oliveira dos Santos; Jordianne Thamires Rodrigues Bezerra; Jobervan Ribeiro Gonçalves Junior ; Amanda Miranda da Silvahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessFerreira, Ana Maria Castro Sousa, Leilane Ribeiro de Amorim, Mateus Sávio Alves, Maria Jucileide Santos, Viviane De Sousa Leal, Joelma Cristina Silva de Jesus Santos, Nayana Mirian Oliveira dos Bezerra, Jordianne Thamires Rodrigues Gonçalves Junior , Jobervan Ribeiro Silva, Amanda Miranda da 2022-08-12T22:23:03Zoai:ojs.pkp.sfu.ca:article/24163Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:42:48.716204Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Microbiological analysis of phytoterapic symptoms (hedera helix l.) for adult/ pediatric use marketed in the city of Teresina-PI Análisis microbiológico de jarabes de fitoterapia (hedera helix l.) para uso adulto/pediátrico vendidos en la ciudad de Teresina-PI Análise microbiológica de xaropes fitoterápicos (hedera helix l.) de uso adulto/pediátrico comercializados na cidade de Teresina-PI |
title |
Microbiological analysis of phytoterapic symptoms (hedera helix l.) for adult/ pediatric use marketed in the city of Teresina-PI |
spellingShingle |
Microbiological analysis of phytoterapic symptoms (hedera helix l.) for adult/ pediatric use marketed in the city of Teresina-PI Ferreira, Ana Maria Castro Microbiological Control Herbal medicines Bacterium. Control Microbiológico Hierbas medicinales Bacterias. Controle Microbiológico Fitoterápicos Bactérias. |
title_short |
Microbiological analysis of phytoterapic symptoms (hedera helix l.) for adult/ pediatric use marketed in the city of Teresina-PI |
title_full |
Microbiological analysis of phytoterapic symptoms (hedera helix l.) for adult/ pediatric use marketed in the city of Teresina-PI |
title_fullStr |
Microbiological analysis of phytoterapic symptoms (hedera helix l.) for adult/ pediatric use marketed in the city of Teresina-PI |
title_full_unstemmed |
Microbiological analysis of phytoterapic symptoms (hedera helix l.) for adult/ pediatric use marketed in the city of Teresina-PI |
title_sort |
Microbiological analysis of phytoterapic symptoms (hedera helix l.) for adult/ pediatric use marketed in the city of Teresina-PI |
author |
Ferreira, Ana Maria Castro |
author_facet |
Ferreira, Ana Maria Castro Sousa, Leilane Ribeiro de Amorim, Mateus Sávio Alves, Maria Jucileide Santos, Viviane De Sousa Leal, Joelma Cristina Silva de Jesus Santos, Nayana Mirian Oliveira dos Bezerra, Jordianne Thamires Rodrigues Gonçalves Junior , Jobervan Ribeiro Silva, Amanda Miranda da |
author_role |
author |
author2 |
Sousa, Leilane Ribeiro de Amorim, Mateus Sávio Alves, Maria Jucileide Santos, Viviane De Sousa Leal, Joelma Cristina Silva de Jesus Santos, Nayana Mirian Oliveira dos Bezerra, Jordianne Thamires Rodrigues Gonçalves Junior , Jobervan Ribeiro Silva, Amanda Miranda da |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Ferreira, Ana Maria Castro Sousa, Leilane Ribeiro de Amorim, Mateus Sávio Alves, Maria Jucileide Santos, Viviane De Sousa Leal, Joelma Cristina Silva de Jesus Santos, Nayana Mirian Oliveira dos Bezerra, Jordianne Thamires Rodrigues Gonçalves Junior , Jobervan Ribeiro Silva, Amanda Miranda da |
dc.subject.por.fl_str_mv |
Microbiological Control Herbal medicines Bacterium. Control Microbiológico Hierbas medicinales Bacterias. Controle Microbiológico Fitoterápicos Bactérias. |
topic |
Microbiological Control Herbal medicines Bacterium. Control Microbiológico Hierbas medicinales Bacterias. Controle Microbiológico Fitoterápicos Bactérias. |
description |
According to DRC No. 26/2014, herbal medicines are a product obtained from active vegetable raw material, with a prophylactic, curative or palliative purpose. The need for microbiological control of these products is important, mainly due to the safety, efficacy and acceptability of these products. The objective of this work was to evaluate the microbiological control of syrups based on the plant Hedera helix L. Two brands of herbal syrups were analyzed. Two dilutions (1:10 and 1: 100) were carried out with neutralization of the preservatives, and subsequent transfer to the enrichment broth, and incubation in the oven at 37 ° C for 24hrs. For the total counting of mesophilic microorganisms, the in-depth method was used for molds and yeasts using a surface method and for the detection of microorganisms, the presence of Escherichia coli, Staphylococcus aureus, Salmonella sp. In sample 1, there was growth of white and yellow colonies at the dilution 10¹, and for dilution at 10², there was no microbial growth. However, for sample 2 at the dilution of 10¹, there were countless small yellow colonies with Gram-positive cocci, while at the 10² dilution there was no growth. For the counting of molds and yeasts, in sample 1 in both dilutions, did not identify fungal form and sample 2 in dilution 10¹ found countless mucoid colonies, with brownish coloration. The present work demonstrated the need for microbiological control of herbal products since the safety, efficacy and acceptability of these products need to be maintained to avoid changes in their physical and chemical properties and to avoid the risk of infection for their users. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-08-12 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/24163 10.33448/rsd-v11i10.24163 |
url |
https://rsdjournal.org/index.php/rsd/article/view/24163 |
identifier_str_mv |
10.33448/rsd-v11i10.24163 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/24163/28127 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 11 No. 10; e37111024163 Research, Society and Development; Vol. 11 Núm. 10; e37111024163 Research, Society and Development; v. 11 n. 10; e37111024163 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052699494055936 |