Antimicrobial and cytotoxic activity of fungal mycelial extracts from aquatic environments in the Amazon

Detalhes bibliográficos
Autor(a) principal: Oliveira, Marta Rodrigues de
Data de Publicação: 2021
Outros Autores: Santiago, Sarah Raquel Silveira da Silva, Katak, Ricardo de Melo, Camargo, Marlene Rodrigues Marcelino, Bastos, Ivanildes dos Santos, Orlandi, Patrícia Puccinelli, Tadei, Wanderli Pedro, Souza, Afonso Duarte Leão de, Souza, Antonia Queiroz Lima de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/18795
Resumo: Fungi are a prolific source of biologically active metabolites, including a wide range of clinically important drugs. Therefore, this study aims to evaluate the antimicrobial and cytotoxic activity of secondary metabolites extracted from fungal mycelia isolated from freshwater samples in the state of Amazonas. Mycelial extracts from 12 fungal were used, extracted with MeOH/AcOEt (1:1) according to the criteria established by Souza et al. (2004). For antimicrobial activity, the extracts were tested against the pathogens Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Enterococcus feacalis, Candida albicans and C. tropicalis. To identify the minimum inhibitory dosage (MID) the microdilution method was used. To perform the cytotoxicity assay, the VERO strain (ATCC® CCL-81TM) was used. The assays were determined by the Alamar Blue method according to Ahmed et al. (1994). The tested extracts did not show antibacterial activity. Five extracts (41.7%), obtained from the fungi Aspergillus - 1283, Chrysoporther - 1169, Diaporther – 1203, Fusarium – 1085, and Trichoderma, showed antifungal activity against C. albicans. Diaporther extract (8.3%) - 1203 was active against C. tropicalis.  In the cytotoxicity assay, 58.3% of the evaluated extracts showed no significant toxic effect. Five extracts, Cladosporium - 1135, Chrysoporther - 1169, Cytospora - 1098, Fusarium - 1085, and Talaromyces - 1244, showed cytotoxic potential, exhibiting viability lower than 70%. The results obtained suggest that mycelial extracts of fungi isolated from water samples from the Amazon region have potential against yeasts of medical interest. Only two of the active extracts were revealed potentially cytotoxic.
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spelling Antimicrobial and cytotoxic activity of fungal mycelial extracts from aquatic environments in the Amazon Actividad antimicrobiana y citotóxica de extractos miceliales de hongos de ambientes acuáticos en la Amazonía Atividade antimicrobiana e citotóxica de extratos miceliais de fungos de ambientes aquáticos da AmazôniaAntifungal activityCytotoxicityBioactive metabolites.Actividad antifúngicaCitotoxicidadMetabolitos bioactivos.Atividade antifúngicaCitotoxicidadeMetabólitos bioativos.Fungi are a prolific source of biologically active metabolites, including a wide range of clinically important drugs. Therefore, this study aims to evaluate the antimicrobial and cytotoxic activity of secondary metabolites extracted from fungal mycelia isolated from freshwater samples in the state of Amazonas. Mycelial extracts from 12 fungal were used, extracted with MeOH/AcOEt (1:1) according to the criteria established by Souza et al. (2004). For antimicrobial activity, the extracts were tested against the pathogens Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Enterococcus feacalis, Candida albicans and C. tropicalis. To identify the minimum inhibitory dosage (MID) the microdilution method was used. To perform the cytotoxicity assay, the VERO strain (ATCC® CCL-81TM) was used. The assays were determined by the Alamar Blue method according to Ahmed et al. (1994). The tested extracts did not show antibacterial activity. Five extracts (41.7%), obtained from the fungi Aspergillus - 1283, Chrysoporther - 1169, Diaporther – 1203, Fusarium – 1085, and Trichoderma, showed antifungal activity against C. albicans. Diaporther extract (8.3%) - 1203 was active against C. tropicalis.  In the cytotoxicity assay, 58.3% of the evaluated extracts showed no significant toxic effect. Five extracts, Cladosporium - 1135, Chrysoporther - 1169, Cytospora - 1098, Fusarium - 1085, and Talaromyces - 1244, showed cytotoxic potential, exhibiting viability lower than 70%. The results obtained suggest that mycelial extracts of fungi isolated from water samples from the Amazon region have potential against yeasts of medical interest. Only two of the active extracts were revealed potentially cytotoxic.Los hongos son una fuente prolífica de metabolitos biológicamente activos, incluida una amplia gama de fármacos clínicamente importantes. Así, el objetivo de este estudio fue evaluar la actividad antimicrobiana y citotóxica de metabolitos secundários extraídos de micelios fúngicos aislados de muestras de agua en el estado de Amazonas. Se utilizaron extractos miceliales de 12 hongos, extraídos con MeOH/ AcOEt (1:1) según los criterios establecidos por Souza et al. (2004). En la prueba antimicrobiana, los extractos fueron evaluados frente a los patógenos Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Enterococcus feacalis, Candida albicans y C. tropicalis. Para identificar la dosis mínima inhibitoria (MID) se utilizó el método de microdilución. Para realizar el ensayo de citotoxicidad se utilizó la cepa VERO (ATCC® CCL-81TM). Los ensayos se determinaron mediante el método Alamar Blue según Ahmed et al. (1994). Los extractos probados no mostraron actividad antibacteriana. Cinco (41,7%) extractos obtenidos de los hongos Aspergillus - 1283, Chrysoporther - 1169, Diaporther – 1203, Fusarium - 1085 y Trichoderma, mostraron actividad antifúngica contra C. albicans y un extracto (8,3%) del hongo Diaporther - 1203 fue activo contra C. tropicalis. En el ensayo de citotoxicidad, el 58,3% de los extractos evaluados no mostraron ningún efecto tóxico significativo y cinco extractos, Cladosporium - 1135, Chrysoporther - 1169, Cytospora - 1098, Fusarium - 1085 y Talaromyces - 1244, mostraron potencial citotóxico com una viabilidad inferior al 70%. Los resultados obtenidos sugieren que extractos miceliales de hongos aislados de muestras de agua de la región amazónica tienen potencial contra levaduras de interés médico. Solo dos de los extractos activos fueron potencialmente citotóxicos.Os fungos são uma fonte prolífica de metabólitos biologicamente ativos, incluindo uma ampla gama de drogas clinicamente importantes. Portanto, este estudo tem como objetivo avaliar a atividade antimicrobiana e citotóxica de metabólitos secundários extraídos de micélios fúngicos isolados de amostras de água doce do estado do Amazonas. Foram utilizados extratos miceliais de 12 fungos, extraídos com MeOH/ AcOEt (1:1) de acordo com os critérios estabelecidos por Souza et al. (2004). Para atividade antimicrobiana, os extratos foram testados contra os patógenos Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Enterococcus feacalis, Candida albicans e C. tropicalis. Para identificar a dosagem inibitória mínima (MID) foi utilizado o método de microdiluição. Para a realização do ensaio de citotoxicidade, foi utilizada a cepa VERO (ATCC® CCL-81TM). Os ensaios foram determinados pelo método Alamar Blue de acordo com Ahmed et al. (1994). Os extratos testados não apresentaram atividade antibacteriana. Cinco extratos (41,7%), obtidos dos fungos Aspergillus - 1283, Chrysoporther - 1169, Diaporther - 1203, Fusarium - 1085 e Trichoderma, apresentaram atividade antifúngica contra C. albicans. O extrato de Diaporther (8,3%) - 1203 foi ativo contra C. tropicalis. No ensaio de citotoxicidade, 58,3% dos extratos avaliados não apresentaram efeito tóxico significativo e cinco extratos, Cladosporium - 1135, Chrysoporther - 1169, Cytospora - 1098, Fusarium - 1085 e Talaromyces - 1244, apresentaram potencial citotóxico exibindo viabilidade menor que 70%. Os resultados obtidos sugerem que extratos miceliais de fungos isolados de amostras de água da região amazônica apresentam potencial contra leveduras de interesse médico. Apenas dois dos extratos ativos foram potencialmente citotóxicos.Research, Society and Development2021-08-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1879510.33448/rsd-v10i10.18795Research, Society and Development; Vol. 10 No. 10; e273101018795Research, Society and Development; Vol. 10 Núm. 10; e273101018795Research, Society and Development; v. 10 n. 10; e2731010187952525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/18795/16820Copyright (c) 2021 Marta Rodrigues de Oliveira; Sarah Raquel Silveira da Silva Santiago; Ricardo de Melo Katak; Marlene Rodrigues Marcelino Camargo; Ivanildes dos Santos Bastos; Patrícia Puccinelli Orlandi; Wanderli Pedro Tadei; Afonso Duarte Leão de Souza; Antonia Queiroz Lima de Souzahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessOliveira, Marta Rodrigues de Santiago, Sarah Raquel Silveira da Silva Katak, Ricardo de Melo Camargo, Marlene Rodrigues Marcelino Bastos, Ivanildes dos Santos Orlandi, Patrícia Puccinelli Tadei, Wanderli Pedro Souza, Afonso Duarte Leão de Souza, Antonia Queiroz Lima de 2021-10-02T21:49:16Zoai:ojs.pkp.sfu.ca:article/18795Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:38:50.694844Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Antimicrobial and cytotoxic activity of fungal mycelial extracts from aquatic environments in the Amazon
Actividad antimicrobiana y citotóxica de extractos miceliales de hongos de ambientes acuáticos en la Amazonía
Atividade antimicrobiana e citotóxica de extratos miceliais de fungos de ambientes aquáticos da Amazônia
title Antimicrobial and cytotoxic activity of fungal mycelial extracts from aquatic environments in the Amazon
spellingShingle Antimicrobial and cytotoxic activity of fungal mycelial extracts from aquatic environments in the Amazon
Oliveira, Marta Rodrigues de
Antifungal activity
Cytotoxicity
Bioactive metabolites.
Actividad antifúngica
Citotoxicidad
Metabolitos bioactivos.
Atividade antifúngica
Citotoxicidade
Metabólitos bioativos.
title_short Antimicrobial and cytotoxic activity of fungal mycelial extracts from aquatic environments in the Amazon
title_full Antimicrobial and cytotoxic activity of fungal mycelial extracts from aquatic environments in the Amazon
title_fullStr Antimicrobial and cytotoxic activity of fungal mycelial extracts from aquatic environments in the Amazon
title_full_unstemmed Antimicrobial and cytotoxic activity of fungal mycelial extracts from aquatic environments in the Amazon
title_sort Antimicrobial and cytotoxic activity of fungal mycelial extracts from aquatic environments in the Amazon
author Oliveira, Marta Rodrigues de
author_facet Oliveira, Marta Rodrigues de
Santiago, Sarah Raquel Silveira da Silva
Katak, Ricardo de Melo
Camargo, Marlene Rodrigues Marcelino
Bastos, Ivanildes dos Santos
Orlandi, Patrícia Puccinelli
Tadei, Wanderli Pedro
Souza, Afonso Duarte Leão de
Souza, Antonia Queiroz Lima de
author_role author
author2 Santiago, Sarah Raquel Silveira da Silva
Katak, Ricardo de Melo
Camargo, Marlene Rodrigues Marcelino
Bastos, Ivanildes dos Santos
Orlandi, Patrícia Puccinelli
Tadei, Wanderli Pedro
Souza, Afonso Duarte Leão de
Souza, Antonia Queiroz Lima de
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Marta Rodrigues de
Santiago, Sarah Raquel Silveira da Silva
Katak, Ricardo de Melo
Camargo, Marlene Rodrigues Marcelino
Bastos, Ivanildes dos Santos
Orlandi, Patrícia Puccinelli
Tadei, Wanderli Pedro
Souza, Afonso Duarte Leão de
Souza, Antonia Queiroz Lima de
dc.subject.por.fl_str_mv Antifungal activity
Cytotoxicity
Bioactive metabolites.
Actividad antifúngica
Citotoxicidad
Metabolitos bioactivos.
Atividade antifúngica
Citotoxicidade
Metabólitos bioativos.
topic Antifungal activity
Cytotoxicity
Bioactive metabolites.
Actividad antifúngica
Citotoxicidad
Metabolitos bioactivos.
Atividade antifúngica
Citotoxicidade
Metabólitos bioativos.
description Fungi are a prolific source of biologically active metabolites, including a wide range of clinically important drugs. Therefore, this study aims to evaluate the antimicrobial and cytotoxic activity of secondary metabolites extracted from fungal mycelia isolated from freshwater samples in the state of Amazonas. Mycelial extracts from 12 fungal were used, extracted with MeOH/AcOEt (1:1) according to the criteria established by Souza et al. (2004). For antimicrobial activity, the extracts were tested against the pathogens Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Enterococcus feacalis, Candida albicans and C. tropicalis. To identify the minimum inhibitory dosage (MID) the microdilution method was used. To perform the cytotoxicity assay, the VERO strain (ATCC® CCL-81TM) was used. The assays were determined by the Alamar Blue method according to Ahmed et al. (1994). The tested extracts did not show antibacterial activity. Five extracts (41.7%), obtained from the fungi Aspergillus - 1283, Chrysoporther - 1169, Diaporther – 1203, Fusarium – 1085, and Trichoderma, showed antifungal activity against C. albicans. Diaporther extract (8.3%) - 1203 was active against C. tropicalis.  In the cytotoxicity assay, 58.3% of the evaluated extracts showed no significant toxic effect. Five extracts, Cladosporium - 1135, Chrysoporther - 1169, Cytospora - 1098, Fusarium - 1085, and Talaromyces - 1244, showed cytotoxic potential, exhibiting viability lower than 70%. The results obtained suggest that mycelial extracts of fungi isolated from water samples from the Amazon region have potential against yeasts of medical interest. Only two of the active extracts were revealed potentially cytotoxic.
publishDate 2021
dc.date.none.fl_str_mv 2021-08-10
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/18795
10.33448/rsd-v10i10.18795
url https://rsdjournal.org/index.php/rsd/article/view/18795
identifier_str_mv 10.33448/rsd-v10i10.18795
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/18795/16820
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 10; e273101018795
Research, Society and Development; Vol. 10 Núm. 10; e273101018795
Research, Society and Development; v. 10 n. 10; e273101018795
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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