Antimicrobial and cytotoxic activity of fungal mycelial extracts from aquatic environments in the Amazon
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/18795 |
Resumo: | Fungi are a prolific source of biologically active metabolites, including a wide range of clinically important drugs. Therefore, this study aims to evaluate the antimicrobial and cytotoxic activity of secondary metabolites extracted from fungal mycelia isolated from freshwater samples in the state of Amazonas. Mycelial extracts from 12 fungal were used, extracted with MeOH/AcOEt (1:1) according to the criteria established by Souza et al. (2004). For antimicrobial activity, the extracts were tested against the pathogens Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Enterococcus feacalis, Candida albicans and C. tropicalis. To identify the minimum inhibitory dosage (MID) the microdilution method was used. To perform the cytotoxicity assay, the VERO strain (ATCC® CCL-81TM) was used. The assays were determined by the Alamar Blue method according to Ahmed et al. (1994). The tested extracts did not show antibacterial activity. Five extracts (41.7%), obtained from the fungi Aspergillus - 1283, Chrysoporther - 1169, Diaporther – 1203, Fusarium – 1085, and Trichoderma, showed antifungal activity against C. albicans. Diaporther extract (8.3%) - 1203 was active against C. tropicalis. In the cytotoxicity assay, 58.3% of the evaluated extracts showed no significant toxic effect. Five extracts, Cladosporium - 1135, Chrysoporther - 1169, Cytospora - 1098, Fusarium - 1085, and Talaromyces - 1244, showed cytotoxic potential, exhibiting viability lower than 70%. The results obtained suggest that mycelial extracts of fungi isolated from water samples from the Amazon region have potential against yeasts of medical interest. Only two of the active extracts were revealed potentially cytotoxic. |
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Antimicrobial and cytotoxic activity of fungal mycelial extracts from aquatic environments in the Amazon Actividad antimicrobiana y citotóxica de extractos miceliales de hongos de ambientes acuáticos en la Amazonía Atividade antimicrobiana e citotóxica de extratos miceliais de fungos de ambientes aquáticos da AmazôniaAntifungal activityCytotoxicityBioactive metabolites.Actividad antifúngicaCitotoxicidadMetabolitos bioactivos.Atividade antifúngicaCitotoxicidadeMetabólitos bioativos.Fungi are a prolific source of biologically active metabolites, including a wide range of clinically important drugs. Therefore, this study aims to evaluate the antimicrobial and cytotoxic activity of secondary metabolites extracted from fungal mycelia isolated from freshwater samples in the state of Amazonas. Mycelial extracts from 12 fungal were used, extracted with MeOH/AcOEt (1:1) according to the criteria established by Souza et al. (2004). For antimicrobial activity, the extracts were tested against the pathogens Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Enterococcus feacalis, Candida albicans and C. tropicalis. To identify the minimum inhibitory dosage (MID) the microdilution method was used. To perform the cytotoxicity assay, the VERO strain (ATCC® CCL-81TM) was used. The assays were determined by the Alamar Blue method according to Ahmed et al. (1994). The tested extracts did not show antibacterial activity. Five extracts (41.7%), obtained from the fungi Aspergillus - 1283, Chrysoporther - 1169, Diaporther – 1203, Fusarium – 1085, and Trichoderma, showed antifungal activity against C. albicans. Diaporther extract (8.3%) - 1203 was active against C. tropicalis. In the cytotoxicity assay, 58.3% of the evaluated extracts showed no significant toxic effect. Five extracts, Cladosporium - 1135, Chrysoporther - 1169, Cytospora - 1098, Fusarium - 1085, and Talaromyces - 1244, showed cytotoxic potential, exhibiting viability lower than 70%. The results obtained suggest that mycelial extracts of fungi isolated from water samples from the Amazon region have potential against yeasts of medical interest. Only two of the active extracts were revealed potentially cytotoxic.Los hongos son una fuente prolífica de metabolitos biológicamente activos, incluida una amplia gama de fármacos clínicamente importantes. Así, el objetivo de este estudio fue evaluar la actividad antimicrobiana y citotóxica de metabolitos secundários extraídos de micelios fúngicos aislados de muestras de agua en el estado de Amazonas. Se utilizaron extractos miceliales de 12 hongos, extraídos con MeOH/ AcOEt (1:1) según los criterios establecidos por Souza et al. (2004). En la prueba antimicrobiana, los extractos fueron evaluados frente a los patógenos Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Enterococcus feacalis, Candida albicans y C. tropicalis. Para identificar la dosis mínima inhibitoria (MID) se utilizó el método de microdilución. Para realizar el ensayo de citotoxicidad se utilizó la cepa VERO (ATCC® CCL-81TM). Los ensayos se determinaron mediante el método Alamar Blue según Ahmed et al. (1994). Los extractos probados no mostraron actividad antibacteriana. Cinco (41,7%) extractos obtenidos de los hongos Aspergillus - 1283, Chrysoporther - 1169, Diaporther – 1203, Fusarium - 1085 y Trichoderma, mostraron actividad antifúngica contra C. albicans y un extracto (8,3%) del hongo Diaporther - 1203 fue activo contra C. tropicalis. En el ensayo de citotoxicidad, el 58,3% de los extractos evaluados no mostraron ningún efecto tóxico significativo y cinco extractos, Cladosporium - 1135, Chrysoporther - 1169, Cytospora - 1098, Fusarium - 1085 y Talaromyces - 1244, mostraron potencial citotóxico com una viabilidad inferior al 70%. Los resultados obtenidos sugieren que extractos miceliales de hongos aislados de muestras de agua de la región amazónica tienen potencial contra levaduras de interés médico. Solo dos de los extractos activos fueron potencialmente citotóxicos.Os fungos são uma fonte prolífica de metabólitos biologicamente ativos, incluindo uma ampla gama de drogas clinicamente importantes. Portanto, este estudo tem como objetivo avaliar a atividade antimicrobiana e citotóxica de metabólitos secundários extraídos de micélios fúngicos isolados de amostras de água doce do estado do Amazonas. Foram utilizados extratos miceliais de 12 fungos, extraídos com MeOH/ AcOEt (1:1) de acordo com os critérios estabelecidos por Souza et al. (2004). Para atividade antimicrobiana, os extratos foram testados contra os patógenos Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Enterococcus feacalis, Candida albicans e C. tropicalis. Para identificar a dosagem inibitória mínima (MID) foi utilizado o método de microdiluição. Para a realização do ensaio de citotoxicidade, foi utilizada a cepa VERO (ATCC® CCL-81TM). Os ensaios foram determinados pelo método Alamar Blue de acordo com Ahmed et al. (1994). Os extratos testados não apresentaram atividade antibacteriana. Cinco extratos (41,7%), obtidos dos fungos Aspergillus - 1283, Chrysoporther - 1169, Diaporther - 1203, Fusarium - 1085 e Trichoderma, apresentaram atividade antifúngica contra C. albicans. O extrato de Diaporther (8,3%) - 1203 foi ativo contra C. tropicalis. No ensaio de citotoxicidade, 58,3% dos extratos avaliados não apresentaram efeito tóxico significativo e cinco extratos, Cladosporium - 1135, Chrysoporther - 1169, Cytospora - 1098, Fusarium - 1085 e Talaromyces - 1244, apresentaram potencial citotóxico exibindo viabilidade menor que 70%. Os resultados obtidos sugerem que extratos miceliais de fungos isolados de amostras de água da região amazônica apresentam potencial contra leveduras de interesse médico. Apenas dois dos extratos ativos foram potencialmente citotóxicos.Research, Society and Development2021-08-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1879510.33448/rsd-v10i10.18795Research, Society and Development; Vol. 10 No. 10; e273101018795Research, Society and Development; Vol. 10 Núm. 10; e273101018795Research, Society and Development; v. 10 n. 10; e2731010187952525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/18795/16820Copyright (c) 2021 Marta Rodrigues de Oliveira; Sarah Raquel Silveira da Silva Santiago; Ricardo de Melo Katak; Marlene Rodrigues Marcelino Camargo; Ivanildes dos Santos Bastos; Patrícia Puccinelli Orlandi; Wanderli Pedro Tadei; Afonso Duarte Leão de Souza; Antonia Queiroz Lima de Souzahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessOliveira, Marta Rodrigues de Santiago, Sarah Raquel Silveira da Silva Katak, Ricardo de Melo Camargo, Marlene Rodrigues Marcelino Bastos, Ivanildes dos Santos Orlandi, Patrícia Puccinelli Tadei, Wanderli Pedro Souza, Afonso Duarte Leão de Souza, Antonia Queiroz Lima de 2021-10-02T21:49:16Zoai:ojs.pkp.sfu.ca:article/18795Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:38:50.694844Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Antimicrobial and cytotoxic activity of fungal mycelial extracts from aquatic environments in the Amazon Actividad antimicrobiana y citotóxica de extractos miceliales de hongos de ambientes acuáticos en la Amazonía Atividade antimicrobiana e citotóxica de extratos miceliais de fungos de ambientes aquáticos da Amazônia |
title |
Antimicrobial and cytotoxic activity of fungal mycelial extracts from aquatic environments in the Amazon |
spellingShingle |
Antimicrobial and cytotoxic activity of fungal mycelial extracts from aquatic environments in the Amazon Oliveira, Marta Rodrigues de Antifungal activity Cytotoxicity Bioactive metabolites. Actividad antifúngica Citotoxicidad Metabolitos bioactivos. Atividade antifúngica Citotoxicidade Metabólitos bioativos. |
title_short |
Antimicrobial and cytotoxic activity of fungal mycelial extracts from aquatic environments in the Amazon |
title_full |
Antimicrobial and cytotoxic activity of fungal mycelial extracts from aquatic environments in the Amazon |
title_fullStr |
Antimicrobial and cytotoxic activity of fungal mycelial extracts from aquatic environments in the Amazon |
title_full_unstemmed |
Antimicrobial and cytotoxic activity of fungal mycelial extracts from aquatic environments in the Amazon |
title_sort |
Antimicrobial and cytotoxic activity of fungal mycelial extracts from aquatic environments in the Amazon |
author |
Oliveira, Marta Rodrigues de |
author_facet |
Oliveira, Marta Rodrigues de Santiago, Sarah Raquel Silveira da Silva Katak, Ricardo de Melo Camargo, Marlene Rodrigues Marcelino Bastos, Ivanildes dos Santos Orlandi, Patrícia Puccinelli Tadei, Wanderli Pedro Souza, Afonso Duarte Leão de Souza, Antonia Queiroz Lima de |
author_role |
author |
author2 |
Santiago, Sarah Raquel Silveira da Silva Katak, Ricardo de Melo Camargo, Marlene Rodrigues Marcelino Bastos, Ivanildes dos Santos Orlandi, Patrícia Puccinelli Tadei, Wanderli Pedro Souza, Afonso Duarte Leão de Souza, Antonia Queiroz Lima de |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Marta Rodrigues de Santiago, Sarah Raquel Silveira da Silva Katak, Ricardo de Melo Camargo, Marlene Rodrigues Marcelino Bastos, Ivanildes dos Santos Orlandi, Patrícia Puccinelli Tadei, Wanderli Pedro Souza, Afonso Duarte Leão de Souza, Antonia Queiroz Lima de |
dc.subject.por.fl_str_mv |
Antifungal activity Cytotoxicity Bioactive metabolites. Actividad antifúngica Citotoxicidad Metabolitos bioactivos. Atividade antifúngica Citotoxicidade Metabólitos bioativos. |
topic |
Antifungal activity Cytotoxicity Bioactive metabolites. Actividad antifúngica Citotoxicidad Metabolitos bioactivos. Atividade antifúngica Citotoxicidade Metabólitos bioativos. |
description |
Fungi are a prolific source of biologically active metabolites, including a wide range of clinically important drugs. Therefore, this study aims to evaluate the antimicrobial and cytotoxic activity of secondary metabolites extracted from fungal mycelia isolated from freshwater samples in the state of Amazonas. Mycelial extracts from 12 fungal were used, extracted with MeOH/AcOEt (1:1) according to the criteria established by Souza et al. (2004). For antimicrobial activity, the extracts were tested against the pathogens Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Enterococcus feacalis, Candida albicans and C. tropicalis. To identify the minimum inhibitory dosage (MID) the microdilution method was used. To perform the cytotoxicity assay, the VERO strain (ATCC® CCL-81TM) was used. The assays were determined by the Alamar Blue method according to Ahmed et al. (1994). The tested extracts did not show antibacterial activity. Five extracts (41.7%), obtained from the fungi Aspergillus - 1283, Chrysoporther - 1169, Diaporther – 1203, Fusarium – 1085, and Trichoderma, showed antifungal activity against C. albicans. Diaporther extract (8.3%) - 1203 was active against C. tropicalis. In the cytotoxicity assay, 58.3% of the evaluated extracts showed no significant toxic effect. Five extracts, Cladosporium - 1135, Chrysoporther - 1169, Cytospora - 1098, Fusarium - 1085, and Talaromyces - 1244, showed cytotoxic potential, exhibiting viability lower than 70%. The results obtained suggest that mycelial extracts of fungi isolated from water samples from the Amazon region have potential against yeasts of medical interest. Only two of the active extracts were revealed potentially cytotoxic. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08-10 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/18795 10.33448/rsd-v10i10.18795 |
url |
https://rsdjournal.org/index.php/rsd/article/view/18795 |
identifier_str_mv |
10.33448/rsd-v10i10.18795 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/18795/16820 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 10; e273101018795 Research, Society and Development; Vol. 10 Núm. 10; e273101018795 Research, Society and Development; v. 10 n. 10; e273101018795 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052753642520576 |