Standardization of an analytical method to quantify ochratoxin A in green coffee beans by high performance liquid chromatography
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Research, Society and Development |
| Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/5070 |
Resumo: | Nowadays, Brazil is the largest producer and exporter of coffee, also the second largest consumer of the beverage. The importance of ensuring food safety for consumers has influenced research to improve and monitor the final product quality. Coffee is a product that presents a high risk of fungal contamination, which can result in the presence of mycotoxins and poses risks to human and animal health. Therefore, this study aimed to standardize a chromatographic method to test and quantify ochratoxin A in 13 samples of green coffee beans. The green coffee beans were stored in sheds without temperature or humidity control. Samples were ground, and the analyte was extracted by a 3% methanol:sodium bicarbonate (1:2 v/v) solution. Ochratoxin A was quantified in a high performance liquid chromatograph. The method was validated by testing the selectivity, linearity, accuracy, limits of detection and quantification. The method presented robustness to the tested parameters and among the analyzed samples. Ochratoxin A was detected above the limit established by the legislation (75.19 µg kg-1) only in one sample. Overall, the storage of green coffee beans in these sheds was adequate, since 12 samples had a low content of ochratoxin A and they were within the limit established by legislation. Therefore, food safety was guaranteed without any severe mycotoxin contamination. |
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Standardization of an analytical method to quantify ochratoxin A in green coffee beans by high performance liquid chromatographyCuantificación de ocratoxina A en granos verdes de café mediante cromatografía líquida de alta eficienciaPadronização de um método analítico para quantificação de ocratoxina A em grãos de café verde por cromatografia líquida de alta eficiênciaHPLCFungoToxinaAlimento.HPLCFungusToxinFood safety.HPLCHongoToxinaAlimento.Nowadays, Brazil is the largest producer and exporter of coffee, also the second largest consumer of the beverage. The importance of ensuring food safety for consumers has influenced research to improve and monitor the final product quality. Coffee is a product that presents a high risk of fungal contamination, which can result in the presence of mycotoxins and poses risks to human and animal health. Therefore, this study aimed to standardize a chromatographic method to test and quantify ochratoxin A in 13 samples of green coffee beans. The green coffee beans were stored in sheds without temperature or humidity control. Samples were ground, and the analyte was extracted by a 3% methanol:sodium bicarbonate (1:2 v/v) solution. Ochratoxin A was quantified in a high performance liquid chromatograph. The method was validated by testing the selectivity, linearity, accuracy, limits of detection and quantification. The method presented robustness to the tested parameters and among the analyzed samples. Ochratoxin A was detected above the limit established by the legislation (75.19 µg kg-1) only in one sample. Overall, the storage of green coffee beans in these sheds was adequate, since 12 samples had a low content of ochratoxin A and they were within the limit established by legislation. Therefore, food safety was guaranteed without any severe mycotoxin contamination.Brasil actualmente es el mayor productor y exportador de café, siendo el segundo mayor consumidor. La importancia de garantizar la seguridad alimentaria al consumidor ha influenciado las investigaciones para mejorar y monitorear la calidad del producto final. El café es uno de los productos más susceptible a ser contaminado por hongos que pueden producir micotoxinas, lo cual representa un riesgo para la salud humana y animal. El objetivo de este trabajo fue estandarizar un método cromatográfico para evaluar y cuantificar ocratoxina A en 13 muestras de granos verdes de café, almacenados en bodegas. Las muestras fueron molidas y el analito fue extraído en solución de metanol:bicarbonato de sodio 3% (1:1 v/v). La cuantificación de ocratoxina A fue realizada mediante un cromatógrafo líquido de alta eficiencia. El método fue validado evaluando los parámetros de selectividad, linealidad, exactitud y límites de detección y cuantificación. El método presentó robustez en los parámetros evaluados, y solamente en una de las muestras analizadas fueron encontrados valores superiores al límite establecido por la legislación (75,19 µg.Kg-1). El almacenamiento de granos de café verde en bodegas mostró ser adecuado en el presente trabajo, pues 12 muestras tuvieron bajos contenidos de ocratoxina A y estuvieron dentro de los límites establecidos por la legislación. De esa forma, se garantiza la seguridad alimentaria sin ninguna contaminación severa por micotoxinas.O Brasil é atualmente o maior produtor e exportador de café, sendo o segundo maior consumidor da bebida. A importância de se garantir a segurança alimentar ao consumidor tem influenciado em pesquisas para melhorar e monitorar a qualidade do produto final. Visto que o café é um dos produtos mais contaminados por fungos que podem produzir micotoxinas, representando riscos à saúde humana e animal. O objetivo do trabalho foi padronizar um método cromatográfico para avaliar e quantificar ocratoxina A em 13 amostras de grãos de café cru armazenados em galpão. As amostras de café foram moídas e o analito foi extraído em solução de metanol:bicarbonato de sódio 3% (1:1 v/v). Foi utilizado um cromatógrafo líquido de alta eficiência para a quantificação da ocratoxina A. O método foi validado avaliando-se os parâmetros de seletividade, linearidade, exatidão e limites de detecção e quantificação. O método apresentou robustez aos parâmetros avaliados e entre amostras analisadas, foram encontrados valores superiores ao limite estabelecido pela legislação somente em uma das amostras (75,19 µg.Kg-1). O método de armazenamento de grãos de café cru em galpão se apresentou adequado no presente trabalho, pois 12 amostras apresentaram baixas concentrações de ocratoxiana A, estando dentro dos limites da legislação. Assim, é garantida a segurança alimentar sem nenhuma contaminação severa de ocratoxina A.Research, Society and Development2020-06-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/507010.33448/rsd-v9i8.5070Research, Society and Development; Vol. 9 No. 8; e39985070Research, Society and Development; Vol. 9 Núm. 8; e39985070Research, Society and Development; v. 9 n. 8; e399850702525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/5070/4387Copyright (c) 2020 Wilder Douglas Santiago, Alexandre Rezende Teixeira, Juliana de Andrade Santiago, Ana Cláudia Alencar Lopes, Rafaela Magalhães Brandão, Richard Bispo Barbosa, Alex Rodrigues Silva Caetano, Maria das Graças Cardoso, Mario Lúcio Vilela Resendeinfo:eu-repo/semantics/openAccessSantiago, Wilder DouglasTeixeira, Alexandre RezendeSantiago, Juliana de AndradeLopes, Ana Cláudia AlencarBrandão, Rafaela MagalhãesBarbosa, Richard BispoCaetano, Alex Rodrigues SilvaCardoso, Maria das GraçasResende, Mario Lúcio Vilela2020-08-20T18:00:17Zoai:ojs.pkp.sfu.ca:article/5070Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:28:42.197442Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
| dc.title.none.fl_str_mv |
Standardization of an analytical method to quantify ochratoxin A in green coffee beans by high performance liquid chromatography Cuantificación de ocratoxina A en granos verdes de café mediante cromatografía líquida de alta eficiencia Padronização de um método analítico para quantificação de ocratoxina A em grãos de café verde por cromatografia líquida de alta eficiência |
| title |
Standardization of an analytical method to quantify ochratoxin A in green coffee beans by high performance liquid chromatography |
| spellingShingle |
Standardization of an analytical method to quantify ochratoxin A in green coffee beans by high performance liquid chromatography Santiago, Wilder Douglas HPLC Fungo Toxina Alimento. HPLC Fungus Toxin Food safety. HPLC Hongo Toxina Alimento. |
| title_short |
Standardization of an analytical method to quantify ochratoxin A in green coffee beans by high performance liquid chromatography |
| title_full |
Standardization of an analytical method to quantify ochratoxin A in green coffee beans by high performance liquid chromatography |
| title_fullStr |
Standardization of an analytical method to quantify ochratoxin A in green coffee beans by high performance liquid chromatography |
| title_full_unstemmed |
Standardization of an analytical method to quantify ochratoxin A in green coffee beans by high performance liquid chromatography |
| title_sort |
Standardization of an analytical method to quantify ochratoxin A in green coffee beans by high performance liquid chromatography |
| author |
Santiago, Wilder Douglas |
| author_facet |
Santiago, Wilder Douglas Teixeira, Alexandre Rezende Santiago, Juliana de Andrade Lopes, Ana Cláudia Alencar Brandão, Rafaela Magalhães Barbosa, Richard Bispo Caetano, Alex Rodrigues Silva Cardoso, Maria das Graças Resende, Mario Lúcio Vilela |
| author_role |
author |
| author2 |
Teixeira, Alexandre Rezende Santiago, Juliana de Andrade Lopes, Ana Cláudia Alencar Brandão, Rafaela Magalhães Barbosa, Richard Bispo Caetano, Alex Rodrigues Silva Cardoso, Maria das Graças Resende, Mario Lúcio Vilela |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Santiago, Wilder Douglas Teixeira, Alexandre Rezende Santiago, Juliana de Andrade Lopes, Ana Cláudia Alencar Brandão, Rafaela Magalhães Barbosa, Richard Bispo Caetano, Alex Rodrigues Silva Cardoso, Maria das Graças Resende, Mario Lúcio Vilela |
| dc.subject.por.fl_str_mv |
HPLC Fungo Toxina Alimento. HPLC Fungus Toxin Food safety. HPLC Hongo Toxina Alimento. |
| topic |
HPLC Fungo Toxina Alimento. HPLC Fungus Toxin Food safety. HPLC Hongo Toxina Alimento. |
| description |
Nowadays, Brazil is the largest producer and exporter of coffee, also the second largest consumer of the beverage. The importance of ensuring food safety for consumers has influenced research to improve and monitor the final product quality. Coffee is a product that presents a high risk of fungal contamination, which can result in the presence of mycotoxins and poses risks to human and animal health. Therefore, this study aimed to standardize a chromatographic method to test and quantify ochratoxin A in 13 samples of green coffee beans. The green coffee beans were stored in sheds without temperature or humidity control. Samples were ground, and the analyte was extracted by a 3% methanol:sodium bicarbonate (1:2 v/v) solution. Ochratoxin A was quantified in a high performance liquid chromatograph. The method was validated by testing the selectivity, linearity, accuracy, limits of detection and quantification. The method presented robustness to the tested parameters and among the analyzed samples. Ochratoxin A was detected above the limit established by the legislation (75.19 µg kg-1) only in one sample. Overall, the storage of green coffee beans in these sheds was adequate, since 12 samples had a low content of ochratoxin A and they were within the limit established by legislation. Therefore, food safety was guaranteed without any severe mycotoxin contamination. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-06-21 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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https://rsdjournal.org/index.php/rsd/article/view/5070 10.33448/rsd-v9i8.5070 |
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https://rsdjournal.org/index.php/rsd/article/view/5070 |
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10.33448/rsd-v9i8.5070 |
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eng |
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eng |
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https://rsdjournal.org/index.php/rsd/article/view/5070/4387 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development; Vol. 9 No. 8; e39985070 Research, Society and Development; Vol. 9 Núm. 8; e39985070 Research, Society and Development; v. 9 n. 8; e39985070 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
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Universidade Federal de Itajubá (UNIFEI) |
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UNIFEI |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
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rsd.articles@gmail.com |
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