Immunohistochemical and histomorphometric analysis of alveolar repair in spontaneously hypertensive rats (SHR) treated with losartan

Detalhes bibliográficos
Autor(a) principal: Pereira, Cassiano Costa Silva
Data de Publicação: 2020
Outros Autores: Manrique, Natália, Moura, Juliana de, Mulinari-Santos, Gabriel, Monteiro, Naara Gabriela, Pitol-Palin, Letícia, Antoniali, Cristina, Okamoto, Roberta
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/10634
Resumo: Hypertension is a multifactorial condition with high rates of complications such as cardiovascular and renal diseases, making it a worldwide public health concern. This disease alters calcium regulation by inducing bone loss, which is limited by anti-hypertensive drugs. One such drug, losartan, inhibits angiotensin II (Ang II) AT1 receptors. The aim of this study was to compare the process of alveolar repair in spontaneously hypertensive rats (SHR) and Wistar rats, and to assess the effect of losartan on bone dynamics. Treated and untreated rats underwent dental extraction of the upper right incisor and were euthanized 7, 14, or 28 days after surgery. Alveolar repair was then analyzed histomorphometrically and immunohistochemically by measuring proteins involved in bone metabolism. Data were analyzed using the nonparametric Kruskal-Wallis test, followed by the Mann Whitney test for comparison of samples at different times. Alveolar repair was slow in SHRs, while losartan increased bone formation and trabecular thickness in both SHRs and Wistars. Because the analyzed proteins are found in dynamic bone, it is suggested that losartan interferes with the actions of angiotensin II and the renin-angiotensin system and limits bone metabolism.
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spelling Immunohistochemical and histomorphometric analysis of alveolar repair in spontaneously hypertensive rats (SHR) treated with losartanAnálisis inmunohistoquímico e histomorfométrico de la reparación alveolar en ratas espontáneamente hipertensas (SHR) tratadas con losartanAnálise imunohistoquímica e histomorfométrica do reparo alveolar em ratos espontaneamente hipertensos (SHR) tratados com losartanLosartanAngiotensin IIBone.LosartánAngiotensina IIHueso.LosartanAngiotensina IIOsso.Hypertension is a multifactorial condition with high rates of complications such as cardiovascular and renal diseases, making it a worldwide public health concern. This disease alters calcium regulation by inducing bone loss, which is limited by anti-hypertensive drugs. One such drug, losartan, inhibits angiotensin II (Ang II) AT1 receptors. The aim of this study was to compare the process of alveolar repair in spontaneously hypertensive rats (SHR) and Wistar rats, and to assess the effect of losartan on bone dynamics. Treated and untreated rats underwent dental extraction of the upper right incisor and were euthanized 7, 14, or 28 days after surgery. Alveolar repair was then analyzed histomorphometrically and immunohistochemically by measuring proteins involved in bone metabolism. Data were analyzed using the nonparametric Kruskal-Wallis test, followed by the Mann Whitney test for comparison of samples at different times. Alveolar repair was slow in SHRs, while losartan increased bone formation and trabecular thickness in both SHRs and Wistars. Because the analyzed proteins are found in dynamic bone, it is suggested that losartan interferes with the actions of angiotensin II and the renin-angiotensin system and limits bone metabolism.La hipertensión es una enfermedad multifactorial con altas tasas de complicaciones, como enfermedades cardiovasculares y renales, lo que la convierte en un problema de salud pública mundial. Esta enfermedad altera la regulación del calcio induciendo la pérdida ósea, que está limitada por fármacos antihipertensivos. Uno de estos fármacos, losartán, inhibe lo receptores AT1 de angiotensina II (Ang II). El objetivo de este estudio fue comparar el proceso de reparación alveolar en ratas espontáneamente hipertensas (SHR) y ratas Wistar, y evaluar el efecto del losartán sobre la dinámica ósea. Las ratas tratadas y no tratadas se sometieron a extracción dental del incisivo superior derecho y se sacrificaron 7, 14 o 28 días después de la cirugía. La reparación alveolar se analizó luego histomorfométricamente e inmunohistoquímicamente midiendo las proteínas implicadas en el metabolismo óseo. Los datos se analizaron mediante la prueba no paramétrica de Kruskal-Wallis, seguida de la prueba de Mann Whitney para comparar muestras en diferentes momentos. La reparación alveolar fue lenta en SHR, mientras que losartán aumentó la formación ósea y el grosor trabecular en SHR y Wistars. Como las proteínas analizadas se encuentran en hueso dinámico, se sugiere que losartán interfiere con las acciones de la angiotensina II y el sistema renina-angiotensina y limita el metabolismo óseo.A hipertensão é uma condição multifatorial com altos índices de complicações, como doenças cardiovasculares e renais, o que a torna um problema de saúde pública mundial. Esta doença altera a regulação do cálcio induzindo a perda óssea, que é limitada por medicamentos anti-hipertensivos. Uma dessas drogas, o losartan, inibe os receptores AT1 da angiotensina II (Ang II). O objetivo deste estudo foi comparar o processo de reparo alveolar em ratos espontaneamente hipertensos (SHR) e ratos Wistar, e avaliar o efeito do losartan na dinâmica óssea. Ratos tratados e não tratados foram submetidos à extração dentária do incisivo superior direito e eutanasiados em 7, 14 ou 28 dias após a cirurgia. O reparo alveolar foi então analisado histomorfometricamente e imunohistoquimicamente através da medição de proteínas envolvidas no metabolismo ósseo. Os dados foram analisados por meio do teste não paramétrico de Kruskal-Wallis, seguido do teste de Mann Whitney para comparação de amostras em momentos diferentes. O reparo alveolar foi lento em SHRs, enquanto o losartan aumentou a formação óssea e a espessura trabecular em SHRs e Wistars. Como as proteínas analisadas são encontradas no osso dinâmico, sugere-se que o losartan interfere nas ações da angiotensina II e do sistema renina-angiotensina e limita o metabolismo ósseo.Research, Society and Development2020-12-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1063410.33448/rsd-v9i11.10634Research, Society and Development; Vol. 9 No. 11; e83991110634Research, Society and Development; Vol. 9 Núm. 11; e83991110634Research, Society and Development; v. 9 n. 11; e839911106342525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/10634/9405Copyright (c) 2020 Cassiano Costa Silva Pereira; Natália Manrique; Juliana de Moura; Gabriel Mulinari-Santos; Naara Gabriela Monteiro; Letícia Pitol-Palin; Cristina Antoniali; Roberta Okamotohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessPereira, Cassiano Costa SilvaManrique, Natália Moura, Juliana de Mulinari-Santos, Gabriel Monteiro, Naara Gabriela Pitol-Palin, Letícia Antoniali, Cristina Okamoto, Roberta 2020-12-10T23:37:57Zoai:ojs.pkp.sfu.ca:article/10634Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:32:39.539234Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Immunohistochemical and histomorphometric analysis of alveolar repair in spontaneously hypertensive rats (SHR) treated with losartan
Análisis inmunohistoquímico e histomorfométrico de la reparación alveolar en ratas espontáneamente hipertensas (SHR) tratadas con losartan
Análise imunohistoquímica e histomorfométrica do reparo alveolar em ratos espontaneamente hipertensos (SHR) tratados com losartan
title Immunohistochemical and histomorphometric analysis of alveolar repair in spontaneously hypertensive rats (SHR) treated with losartan
spellingShingle Immunohistochemical and histomorphometric analysis of alveolar repair in spontaneously hypertensive rats (SHR) treated with losartan
Pereira, Cassiano Costa Silva
Losartan
Angiotensin II
Bone.
Losartán
Angiotensina II
Hueso.
Losartan
Angiotensina II
Osso.
title_short Immunohistochemical and histomorphometric analysis of alveolar repair in spontaneously hypertensive rats (SHR) treated with losartan
title_full Immunohistochemical and histomorphometric analysis of alveolar repair in spontaneously hypertensive rats (SHR) treated with losartan
title_fullStr Immunohistochemical and histomorphometric analysis of alveolar repair in spontaneously hypertensive rats (SHR) treated with losartan
title_full_unstemmed Immunohistochemical and histomorphometric analysis of alveolar repair in spontaneously hypertensive rats (SHR) treated with losartan
title_sort Immunohistochemical and histomorphometric analysis of alveolar repair in spontaneously hypertensive rats (SHR) treated with losartan
author Pereira, Cassiano Costa Silva
author_facet Pereira, Cassiano Costa Silva
Manrique, Natália
Moura, Juliana de
Mulinari-Santos, Gabriel
Monteiro, Naara Gabriela
Pitol-Palin, Letícia
Antoniali, Cristina
Okamoto, Roberta
author_role author
author2 Manrique, Natália
Moura, Juliana de
Mulinari-Santos, Gabriel
Monteiro, Naara Gabriela
Pitol-Palin, Letícia
Antoniali, Cristina
Okamoto, Roberta
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pereira, Cassiano Costa Silva
Manrique, Natália
Moura, Juliana de
Mulinari-Santos, Gabriel
Monteiro, Naara Gabriela
Pitol-Palin, Letícia
Antoniali, Cristina
Okamoto, Roberta
dc.subject.por.fl_str_mv Losartan
Angiotensin II
Bone.
Losartán
Angiotensina II
Hueso.
Losartan
Angiotensina II
Osso.
topic Losartan
Angiotensin II
Bone.
Losartán
Angiotensina II
Hueso.
Losartan
Angiotensina II
Osso.
description Hypertension is a multifactorial condition with high rates of complications such as cardiovascular and renal diseases, making it a worldwide public health concern. This disease alters calcium regulation by inducing bone loss, which is limited by anti-hypertensive drugs. One such drug, losartan, inhibits angiotensin II (Ang II) AT1 receptors. The aim of this study was to compare the process of alveolar repair in spontaneously hypertensive rats (SHR) and Wistar rats, and to assess the effect of losartan on bone dynamics. Treated and untreated rats underwent dental extraction of the upper right incisor and were euthanized 7, 14, or 28 days after surgery. Alveolar repair was then analyzed histomorphometrically and immunohistochemically by measuring proteins involved in bone metabolism. Data were analyzed using the nonparametric Kruskal-Wallis test, followed by the Mann Whitney test for comparison of samples at different times. Alveolar repair was slow in SHRs, while losartan increased bone formation and trabecular thickness in both SHRs and Wistars. Because the analyzed proteins are found in dynamic bone, it is suggested that losartan interferes with the actions of angiotensin II and the renin-angiotensin system and limits bone metabolism.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-05
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/10634
10.33448/rsd-v9i11.10634
url https://rsdjournal.org/index.php/rsd/article/view/10634
identifier_str_mv 10.33448/rsd-v9i11.10634
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/10634/9405
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 9 No. 11; e83991110634
Research, Society and Development; Vol. 9 Núm. 11; e83991110634
Research, Society and Development; v. 9 n. 11; e83991110634
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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