Antimicrobial action of volatile compounds in essential oil from dry leaves of Croton blanchetianus Baill
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/24785 |
Resumo: | Croton blachetianus, popularly called marmeleiro, is a shrub rich in secondary metabolites that has several biological activities. The aim of this study was to determine the composition of volatile compounds in the essential oil of dry leaves of C. blanchetianus and its antimicrobial action (minimum inhibitory dose – vapor DMI) on lactic acid bacteria (LAB), Weissella viridescens and Leuconostoc mesenteroides, spoilage bacteria for meat products. The essential oil from the dry leaves of C. blanchetianus was extracted by the hydrodistillation technique and the volatile compounds were analyzed by CG headspace technique. The minimum inhibitory dose in the vapor phase was defined as the minimum dose of an essential oil solution of C. blanchetianus in 5% DMSO whose vapor completely inhibits BAL’s growth for 48 hours. Subsequently, the bactericidal and bacteriostatic effect was determined. The major compounds found were eucalyptol (32.94%) and D-α-Pinene (29.43%) terpenes. The minimum inhibitory dose was 0.32g/mL for both microorganisms. The bactericidal effect for W. viridescens was observed by the action of vapor from the 0.32 g/mL solution and for L. mesenteroides the bacteriostatic and bactericidal effect was observed by the action of vapor from the 0.40 g/mL and 0.57 solution g/ml, respectively. Thus, it was possible to observe the presence of terpenes, as major compounds in the headspace analysis, as well as the antimicrobial, bacteriostatic and bactericidal activity of the essential oil from dry leaves of C. blanchetianus against BAL W. viridescens and L. mesenteroides. |
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Antimicrobial action of volatile compounds in essential oil from dry leaves of Croton blanchetianus BaillAcción antimicrobiana de compuestos volátiles en aceite esencial de hojas secas de Croton blanchetianus BaillAção antimicrobiana dos compostos voláteis do óleo essencial das folhas secas de Croton blanchetianus BaillHeadspaceAntimicrobianoBacteriostáticoBactericida.HeadspaceAntimicrobialBacteriostaticBactericide.HeadspaceAntimicrobianoBacteriostáticoBactericida.Croton blachetianus, popularly called marmeleiro, is a shrub rich in secondary metabolites that has several biological activities. The aim of this study was to determine the composition of volatile compounds in the essential oil of dry leaves of C. blanchetianus and its antimicrobial action (minimum inhibitory dose – vapor DMI) on lactic acid bacteria (LAB), Weissella viridescens and Leuconostoc mesenteroides, spoilage bacteria for meat products. The essential oil from the dry leaves of C. blanchetianus was extracted by the hydrodistillation technique and the volatile compounds were analyzed by CG headspace technique. The minimum inhibitory dose in the vapor phase was defined as the minimum dose of an essential oil solution of C. blanchetianus in 5% DMSO whose vapor completely inhibits BAL’s growth for 48 hours. Subsequently, the bactericidal and bacteriostatic effect was determined. The major compounds found were eucalyptol (32.94%) and D-α-Pinene (29.43%) terpenes. The minimum inhibitory dose was 0.32g/mL for both microorganisms. The bactericidal effect for W. viridescens was observed by the action of vapor from the 0.32 g/mL solution and for L. mesenteroides the bacteriostatic and bactericidal effect was observed by the action of vapor from the 0.40 g/mL and 0.57 solution g/ml, respectively. Thus, it was possible to observe the presence of terpenes, as major compounds in the headspace analysis, as well as the antimicrobial, bacteriostatic and bactericidal activity of the essential oil from dry leaves of C. blanchetianus against BAL W. viridescens and L. mesenteroides.Croton blachetianus, popularmente llamado marmeleiro, es un arbusto rico en metabolitos secundarios que tiene varias actividades biológicas. El objetivo de este estudio fue determinar la composición de compuestos volátiles en el aceite esencial de hojas secas de C. blanchetianus y su acción antimicrobiana (dosis mínima inhibitoria – DMI de vapor) sobre bacterias ácido lácticas (BAL), cepas Weissella viridescens y de Leuconostoc mesenteroides, deterioro de productos cárnicos. El aceite esencial de las hojas secas de C. blanchetianus se extrajo mediante la técnica de hidrodestilación y los compuestos volátiles se analizaron mediante la técnica de headspace, mediante cromatografía de gases. La DMI en la fase de vapor se definió como la dosis mínima de una solución de aceite esencial de C. blanchetianus en DMSO al 5% cuyo vapor inhibe completamente el crecimiento de BAL durante 48 horas. Posteriormente, se determinó el efecto bactericida y bacteriostático. Los principales compuestos encontrados fueron eucaliptol (32.94%) y terpenos D-α-Pineno (29.43%). La DMI fue de 0.32 g/mL para ambos microorganismos. El efecto bactericida para W. viridescens se observó por la acción del vapor de la solución 0.32 g/mL y para L. mesenteroides el efecto bacteriostático y bactericida se observó por la acción del vapor de la solución 0.40 g/mL y 0.57 g/ml, respectivamente. Así, fue posible observar la presencia de terpenos como compuestos principales en el análisis del headspace, así como la actividad antimicrobiana, bacteriostática y bactericida del aceite esencial de hojas secas de C. blanchetianus contra las BAL W. viridescens y L. mesenteroides.O Croton blachetianus, popularmente denominado marmeleiro, é um arbusto rico em metabólitos secundários que apresenta diversas atividades biológicas. O objetivo desse estudo foi determinar a composição dos compostos voláteis do óleo essencial das folhas secas de C. blanchetianus e sua ação antimicrobiana (dose mínima inibitória – DMI do vapor) sobre as cepas das bactérias ácido-lácticas (BAL), Weissella viridescens e Leuconostoc mesenteroides, deteriorantes de produtos cárneos. O óleo essencial das folhas secas de C. blanchetianus foi extraído pela técnica de hidrodestilação e os compostos voláteis foram analisados pela técnica de headspace, por cromatografia gasosa. A dose mínima inibitória na fase vapor foi definida como a dose mínima de solução de óleo essencial de C. blanchetianus em DMSO 5% cujo vapor inibe completamente o crescimento das BAL, durante 48 horas. Posteriormente, o efeito bactericida e bacteriostático foi determinado. Os compostos majoritários encontrados foram os terpenos eucaliptol (32,94%) e D-α-Pineno (29,43%). A dose mínima inibitória foi de 0,32g/mL, para ambos os microrganismos. O efeito bactericida para W. viridescens foi observado pela ação do vapor da solução de 0,32 g/mL e, para a L. mesenteroides, o efeito bacteriostático e bactericida foi pela ação do vapor da solução de 0,40 g/mL e 0,57 g/mL, respectivamente. Assim, foi possível observar a presença de terpenos como compostos majoritários na análise por headspace, bem como a atividade antimicrobiana, bacteriostática e bactericida do óleo essencial das folhas secas de C. blanchetianus frente as BAL W. viridescens e L. mesenteroides.Research, Society and Development2022-01-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2478510.33448/rsd-v11i1.24785Research, Society and Development; Vol. 11 No. 1; e28011124785Research, Society and Development; Vol. 11 Núm. 1; e28011124785Research, Society and Development; v. 11 n. 1; e280111247852525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/24785/21880Copyright (c) 2022 Elayne Cardoso de Vasconcelos; Camila Casagrande Paganini; Evânia Altina Teixeira de Figueiredo; Gláucia Maria Falcão de Aragãohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessVasconcelos, Elayne Cardoso de Paganini, Camila Casagrande Figueiredo, Evânia Altina Teixeira de Aragão, Gláucia Maria Falcão de 2022-01-16T18:08:18Zoai:ojs.pkp.sfu.ca:article/24785Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:43:13.856139Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Antimicrobial action of volatile compounds in essential oil from dry leaves of Croton blanchetianus Baill Acción antimicrobiana de compuestos volátiles en aceite esencial de hojas secas de Croton blanchetianus Baill Ação antimicrobiana dos compostos voláteis do óleo essencial das folhas secas de Croton blanchetianus Baill |
title |
Antimicrobial action of volatile compounds in essential oil from dry leaves of Croton blanchetianus Baill |
spellingShingle |
Antimicrobial action of volatile compounds in essential oil from dry leaves of Croton blanchetianus Baill Vasconcelos, Elayne Cardoso de Headspace Antimicrobiano Bacteriostático Bactericida. Headspace Antimicrobial Bacteriostatic Bactericide. Headspace Antimicrobiano Bacteriostático Bactericida. |
title_short |
Antimicrobial action of volatile compounds in essential oil from dry leaves of Croton blanchetianus Baill |
title_full |
Antimicrobial action of volatile compounds in essential oil from dry leaves of Croton blanchetianus Baill |
title_fullStr |
Antimicrobial action of volatile compounds in essential oil from dry leaves of Croton blanchetianus Baill |
title_full_unstemmed |
Antimicrobial action of volatile compounds in essential oil from dry leaves of Croton blanchetianus Baill |
title_sort |
Antimicrobial action of volatile compounds in essential oil from dry leaves of Croton blanchetianus Baill |
author |
Vasconcelos, Elayne Cardoso de |
author_facet |
Vasconcelos, Elayne Cardoso de Paganini, Camila Casagrande Figueiredo, Evânia Altina Teixeira de Aragão, Gláucia Maria Falcão de |
author_role |
author |
author2 |
Paganini, Camila Casagrande Figueiredo, Evânia Altina Teixeira de Aragão, Gláucia Maria Falcão de |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Vasconcelos, Elayne Cardoso de Paganini, Camila Casagrande Figueiredo, Evânia Altina Teixeira de Aragão, Gláucia Maria Falcão de |
dc.subject.por.fl_str_mv |
Headspace Antimicrobiano Bacteriostático Bactericida. Headspace Antimicrobial Bacteriostatic Bactericide. Headspace Antimicrobiano Bacteriostático Bactericida. |
topic |
Headspace Antimicrobiano Bacteriostático Bactericida. Headspace Antimicrobial Bacteriostatic Bactericide. Headspace Antimicrobiano Bacteriostático Bactericida. |
description |
Croton blachetianus, popularly called marmeleiro, is a shrub rich in secondary metabolites that has several biological activities. The aim of this study was to determine the composition of volatile compounds in the essential oil of dry leaves of C. blanchetianus and its antimicrobial action (minimum inhibitory dose – vapor DMI) on lactic acid bacteria (LAB), Weissella viridescens and Leuconostoc mesenteroides, spoilage bacteria for meat products. The essential oil from the dry leaves of C. blanchetianus was extracted by the hydrodistillation technique and the volatile compounds were analyzed by CG headspace technique. The minimum inhibitory dose in the vapor phase was defined as the minimum dose of an essential oil solution of C. blanchetianus in 5% DMSO whose vapor completely inhibits BAL’s growth for 48 hours. Subsequently, the bactericidal and bacteriostatic effect was determined. The major compounds found were eucalyptol (32.94%) and D-α-Pinene (29.43%) terpenes. The minimum inhibitory dose was 0.32g/mL for both microorganisms. The bactericidal effect for W. viridescens was observed by the action of vapor from the 0.32 g/mL solution and for L. mesenteroides the bacteriostatic and bactericidal effect was observed by the action of vapor from the 0.40 g/mL and 0.57 solution g/ml, respectively. Thus, it was possible to observe the presence of terpenes, as major compounds in the headspace analysis, as well as the antimicrobial, bacteriostatic and bactericidal activity of the essential oil from dry leaves of C. blanchetianus against BAL W. viridescens and L. mesenteroides. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-06 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/24785 10.33448/rsd-v11i1.24785 |
url |
https://rsdjournal.org/index.php/rsd/article/view/24785 |
identifier_str_mv |
10.33448/rsd-v11i1.24785 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/24785/21880 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 11 No. 1; e28011124785 Research, Society and Development; Vol. 11 Núm. 1; e28011124785 Research, Society and Development; v. 11 n. 1; e28011124785 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052810361044992 |