Investigation of Leifsonia xyli subsp. xyli in insects

Detalhes bibliográficos
Autor(a) principal: Panta, Airan Miguel dos Santos
Data de Publicação: 2022
Outros Autores: Souza, Juliana Lopes, Gagliardi, Paulo Roberto, Café, Lilian Pinheiro Santos, Silva-Mann, Renata
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/27469
Resumo: Leifsonia xyli subsp. xyli, the causal agent of ratoon stunting, is an endophytic bacteria that colonize sugarcane xylem vessels. It is generally accepted that sugarcane is the only natural host of ratoon stunting. The objective of this work was to investigate the presence of bacteria in insects associated with sugarcane in an experimental area cultivated with four varieties. The plants were artificially inoculated with bacteria, the samples of insects were collected from the sugarcane crop. Specific PCR was used with DNA extracted from different insect parts (head, abdomen, or whole-body). The DNA of the bacterium was not detected in those investigated insects, confirming that the host range of the bacterium is restricted to Saccharum spp.
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spelling Investigation of Leifsonia xyli subsp. xyli in insectsInvestigación de Leifsonia xyli subsp. xyli en insectos Investigação de Leifsonia xyli subsp. xyli em insetos SaccharumADNRaquitismo de retoño.Saccharum, ADN, raquitismo de retoñoSaccharumDNASugarcane ratoon stunting.Leifsonia xyli subsp. xyli, the causal agent of ratoon stunting, is an endophytic bacteria that colonize sugarcane xylem vessels. It is generally accepted that sugarcane is the only natural host of ratoon stunting. The objective of this work was to investigate the presence of bacteria in insects associated with sugarcane in an experimental area cultivated with four varieties. The plants were artificially inoculated with bacteria, the samples of insects were collected from the sugarcane crop. Specific PCR was used with DNA extracted from different insect parts (head, abdomen, or whole-body). The DNA of the bacterium was not detected in those investigated insects, confirming that the host range of the bacterium is restricted to Saccharum spp.Leifsonia xyli subesp. xyli, el agente causal del retraso del crecimiento en ratas, es una bacteria endofítica que coloniza los vasos del xilema de la caña de azúcar. En general, se acepta que la caña de azúcar es el único huésped natural del retraso en el crecimiento de los retoños. El objetivo de este trabajo fue investigar la presencia de bacterias en insectos asociados a la caña de azúcar en un área experimental cultivada con cuatro variedades. Las plantas fueron inoculadas artificialmente con bacterias, las muestras de insectos fueron recolectadas del cultivo de caña de azúcar. Se utilizó PCR específica con ADN extraído de diferentes partes del insecto (cabeza, abdomen o cuerpo entero). El ADN de la bacteria no se detectó en los insectos investigados, lo que confirma que el rango de huéspedes de la bacteria está restringido a Saccharum spp.Leifsonia xyli subsp. xyli, o agente causal do nanismo de ratos, é uma bactéria endofítica que coloniza os vasos do xilema da cana-de-açúcar. É geralmente aceito que a cana-de-açúcar é o único hospedeiro natural do raquitismo da soca. O objetivo deste trabalho foi investigar a presença de bactérias em insetos associados à cana-de-açúcar em uma área experimental cultivada com quatro variedades. As plantas foram inoculadas artificialmente com bactérias, as amostras de insetos foram coletadas da cultura da cana-de-açúcar. PCR específico foi usado com DNA extraído de diferentes partes do inseto (cabeça, abdômen ou corpo inteiro). O DNA da bactéria não foi detectado nos insetos investigados, confirmando que a gama de hospedeiros da bactéria é restrita a Saccharum spp.Research, Society and Development2022-04-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2746910.33448/rsd-v11i5.27469Research, Society and Development; Vol. 11 No. 5; e13311527469Research, Society and Development; Vol. 11 Núm. 5; e13311527469Research, Society and Development; v. 11 n. 5; e133115274692525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/27469/24915Copyright (c) 2022 Airan Miguel dos Santos Panta; Juliana Lopes Souza; Paulo Roberto Gagliardi; Lilian Pinheiro Santos Café; Renata Silva-Mannhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessPanta, Airan Miguel dos Santos Souza, Juliana Lopes Gagliardi, Paulo Roberto Café, Lilian Pinheiro Santos Silva-Mann, Renata2022-04-17T18:18:56Zoai:ojs.pkp.sfu.ca:article/27469Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:45:12.105554Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Investigation of Leifsonia xyli subsp. xyli in insects
Investigación de Leifsonia xyli subsp. xyli en insectos
Investigação de Leifsonia xyli subsp. xyli em insetos
title Investigation of Leifsonia xyli subsp. xyli in insects
spellingShingle Investigation of Leifsonia xyli subsp. xyli in insects
Panta, Airan Miguel dos Santos
Saccharum
ADN
Raquitismo de retoño.
Saccharum, ADN, raquitismo de retoño
Saccharum
DNA
Sugarcane ratoon stunting.
title_short Investigation of Leifsonia xyli subsp. xyli in insects
title_full Investigation of Leifsonia xyli subsp. xyli in insects
title_fullStr Investigation of Leifsonia xyli subsp. xyli in insects
title_full_unstemmed Investigation of Leifsonia xyli subsp. xyli in insects
title_sort Investigation of Leifsonia xyli subsp. xyli in insects
author Panta, Airan Miguel dos Santos
author_facet Panta, Airan Miguel dos Santos
Souza, Juliana Lopes
Gagliardi, Paulo Roberto
Café, Lilian Pinheiro Santos
Silva-Mann, Renata
author_role author
author2 Souza, Juliana Lopes
Gagliardi, Paulo Roberto
Café, Lilian Pinheiro Santos
Silva-Mann, Renata
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Panta, Airan Miguel dos Santos
Souza, Juliana Lopes
Gagliardi, Paulo Roberto
Café, Lilian Pinheiro Santos
Silva-Mann, Renata
dc.subject.por.fl_str_mv Saccharum
ADN
Raquitismo de retoño.
Saccharum, ADN, raquitismo de retoño
Saccharum
DNA
Sugarcane ratoon stunting.
topic Saccharum
ADN
Raquitismo de retoño.
Saccharum, ADN, raquitismo de retoño
Saccharum
DNA
Sugarcane ratoon stunting.
description Leifsonia xyli subsp. xyli, the causal agent of ratoon stunting, is an endophytic bacteria that colonize sugarcane xylem vessels. It is generally accepted that sugarcane is the only natural host of ratoon stunting. The objective of this work was to investigate the presence of bacteria in insects associated with sugarcane in an experimental area cultivated with four varieties. The plants were artificially inoculated with bacteria, the samples of insects were collected from the sugarcane crop. Specific PCR was used with DNA extracted from different insect parts (head, abdomen, or whole-body). The DNA of the bacterium was not detected in those investigated insects, confirming that the host range of the bacterium is restricted to Saccharum spp.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-18
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/27469
10.33448/rsd-v11i5.27469
url https://rsdjournal.org/index.php/rsd/article/view/27469
identifier_str_mv 10.33448/rsd-v11i5.27469
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/27469/24915
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 11 No. 5; e13311527469
Research, Society and Development; Vol. 11 Núm. 5; e13311527469
Research, Society and Development; v. 11 n. 5; e13311527469
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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