Sequential production of two biopolymers-polyhydroxyalkanoate and levan by microbial fermentation
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/40745 |
Resumo: | The sequential production of polyhydroxyalkanoates (PHA) and levan was investigated by microbial fermentation using agro-industrial residues. PHA production was carried out by Cupriavidus necator DSMZ 545 in two steps: a) bacterial growth, using pure (MN) and hydrolyzed (MH) molasses and; b) accumulation of PHA, using standard glycerol (GP) and crude glycerol (GB). The fermented remaining from the C. necator growth stage was separated from the PHA-containing biomass and used in the subsequent production of levan by Bacillus subtilis (natto) Takahashi. The medium containing MH+GP generated a rate of 15 mg of PHA, while the medium based on MH+GB the rate of PHA was almost twice as high (28.4 mg). FTIR spectroscopic analysis of PHA indicated stretching vibrations characteristic for a PHB-like molecule. In turn, the levan produced in this study was precipitated with different volumes of ethanol, generating levans with different molecular weights. The results of the characterization of this fructan by chromatography showed that it was predominantly constituted by fructose units. In addition, the dynamic and kinematic viscosity values for levan were similar in the analyzed concentrations and, therefore, did not provide any clues about the molecular weight of this biopolymer. Finally, the sequential production process of PHA and levan, as a second bioproduct, represents an elegant alternative to reduce the total costs of PHA production. |
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Sequential production of two biopolymers-polyhydroxyalkanoate and levan by microbial fermentationProducción secuencial de dos biopolímeros-polihidroxialcanoato y leván por fermentación microbianaProdução sequencial de dois biopolímeros-polihidroxialcanoato e levana por fermentação microbianaPolihidroxialcanoatosLevanaFermentaçãoCupriavidus necatorBacillus subtilis natto.PolihidroxialcanoatosLevanaFermentaciónCupriavidus necatorBacillus subtilis natto.PolyhydroxyalkanoatesLevanaFermentationCupriavidus necatorBacillus subtilis natto.The sequential production of polyhydroxyalkanoates (PHA) and levan was investigated by microbial fermentation using agro-industrial residues. PHA production was carried out by Cupriavidus necator DSMZ 545 in two steps: a) bacterial growth, using pure (MN) and hydrolyzed (MH) molasses and; b) accumulation of PHA, using standard glycerol (GP) and crude glycerol (GB). The fermented remaining from the C. necator growth stage was separated from the PHA-containing biomass and used in the subsequent production of levan by Bacillus subtilis (natto) Takahashi. The medium containing MH+GP generated a rate of 15 mg of PHA, while the medium based on MH+GB the rate of PHA was almost twice as high (28.4 mg). FTIR spectroscopic analysis of PHA indicated stretching vibrations characteristic for a PHB-like molecule. In turn, the levan produced in this study was precipitated with different volumes of ethanol, generating levans with different molecular weights. The results of the characterization of this fructan by chromatography showed that it was predominantly constituted by fructose units. In addition, the dynamic and kinematic viscosity values for levan were similar in the analyzed concentrations and, therefore, did not provide any clues about the molecular weight of this biopolymer. Finally, the sequential production process of PHA and levan, as a second bioproduct, represents an elegant alternative to reduce the total costs of PHA production.Se investigó la producción secuencial de polihidroxialcanoatos (PHA) y leván mediante fermentación microbiana utilizando residuos agroindustriales. La producción de PHA fue realizada por Cupriavidus necator DSMZ 545 en dos pasos: a) crecimiento bacteriano, utilizando melaza pura (MN) e hidrolizada (MH) y; b) acumulación de PHA, utilizando glicerol estándar (GP) y glicerol crudo (GB). El resto fermentado de la etapa de crecimiento de C. necator se separó de la biomasa que contenía PHA y se usó en la producción posterior de levan por Bacillus subtilis (natto) Takahashi. El medio que contenía MH+GP generó una tasa de 15 mg de PHA, mientras que el medio basado en MH+GB la tasa de PHA fue casi el doble (28,4 mg). El análisis espectroscópico FTIR de PHA indicó vibraciones de estiramiento características de una molécula similar a PHB. A su vez, el leván producido en este estudio se precipitó con diferentes volúmenes de etanol, generando leván con diferentes pesos moleculares. Los resultados de la caracterización de este fructano por cromatografía mostraron que estaba predominantemente constituido por unidades de fructosa. Además, los valores de viscosidad dinámica y cinemática del leván fueron similares en las concentraciones analizadas y, por tanto, no aportaron pistas sobre el peso molecular de este biopolímero. Finalmente, el proceso de producción secuencial de PHA y leván, como segundo bioproducto, representa una alternativa elegante para reducir los costos totales de producción de PHA.A produção sequencial de polihidroxialcanoatos (PHA) e levana foi investigada por fermentação microbiana usando resíduos agroindustriais. A produção de PHA foi realizada por Cupriavidus necator DSMZ 545 em duas etapas: a) crescimento bacteriano, usando melaço puro (MN) e hidrolisado (MH) e; b) acumulação de PHA, usando glicerol padrão (GP) e glicerol bruto (GB). O fermentado remanescente da etapa de crescimento de C. necator foi separado da biomassa contendo PHA e usado na produção subsequente de levana por Bacillus subtilis (natto) Takahashi. O meio contendo MH+GP gerou uma taxa de 15 mg de PHA, enquanto o meio à base de MH+GB a taxa de PHA foi quase duas vezes maior (28,4 mg). A análise espectroscópica por FTIR de PHA indicou vibrações de estiramentos característicos para uma molécula do tipo PHB. Por sua vez, levana produzida neste estudo foi precipitada com diferentes volumes de etanol, gerando levanas com diferentes pesos moleculares. Os resultados da caracterização desta frutana por cromatografia mostrou que a mesma era constituída predominantemente por unidades de frutose. Além disso, os valores de viscosidade dinâmica e cinemática para a levana foram semelhantes nas concentrações analisadas e, portanto, não forneceram indícios acerca do peso molecular desse biopolímero. Por fim, o processo de produção sequencial de PHA e levana, como um segundo bioproduto, representa uma alternativa elegante para reduzir os custos totais de produção do PHA.Research, Society and Development2023-03-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/4074510.33448/rsd-v12i3.40745Research, Society and Development; Vol. 12 No. 3; e25212340745Research, Society and Development; Vol. 12 Núm. 3; e25212340745Research, Society and Development; v. 12 n. 3; e252123407452525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/40745/33242Copyright (c) 2023 Edmilson Clarindo de Siqueira; Laureen Michelle Houllou https://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSiqueira, Edmilson Clarindo de Houllou , Laureen Michelle 2023-03-23T08:33:38Zoai:ojs.pkp.sfu.ca:article/40745Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2023-03-23T08:33:38Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Sequential production of two biopolymers-polyhydroxyalkanoate and levan by microbial fermentation Producción secuencial de dos biopolímeros-polihidroxialcanoato y leván por fermentación microbiana Produção sequencial de dois biopolímeros-polihidroxialcanoato e levana por fermentação microbiana |
title |
Sequential production of two biopolymers-polyhydroxyalkanoate and levan by microbial fermentation |
spellingShingle |
Sequential production of two biopolymers-polyhydroxyalkanoate and levan by microbial fermentation Siqueira, Edmilson Clarindo de Polihidroxialcanoatos Levana Fermentação Cupriavidus necator Bacillus subtilis natto. Polihidroxialcanoatos Levana Fermentación Cupriavidus necator Bacillus subtilis natto. Polyhydroxyalkanoates Levana Fermentation Cupriavidus necator Bacillus subtilis natto. |
title_short |
Sequential production of two biopolymers-polyhydroxyalkanoate and levan by microbial fermentation |
title_full |
Sequential production of two biopolymers-polyhydroxyalkanoate and levan by microbial fermentation |
title_fullStr |
Sequential production of two biopolymers-polyhydroxyalkanoate and levan by microbial fermentation |
title_full_unstemmed |
Sequential production of two biopolymers-polyhydroxyalkanoate and levan by microbial fermentation |
title_sort |
Sequential production of two biopolymers-polyhydroxyalkanoate and levan by microbial fermentation |
author |
Siqueira, Edmilson Clarindo de |
author_facet |
Siqueira, Edmilson Clarindo de Houllou , Laureen Michelle |
author_role |
author |
author2 |
Houllou , Laureen Michelle |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Siqueira, Edmilson Clarindo de Houllou , Laureen Michelle |
dc.subject.por.fl_str_mv |
Polihidroxialcanoatos Levana Fermentação Cupriavidus necator Bacillus subtilis natto. Polihidroxialcanoatos Levana Fermentación Cupriavidus necator Bacillus subtilis natto. Polyhydroxyalkanoates Levana Fermentation Cupriavidus necator Bacillus subtilis natto. |
topic |
Polihidroxialcanoatos Levana Fermentação Cupriavidus necator Bacillus subtilis natto. Polihidroxialcanoatos Levana Fermentación Cupriavidus necator Bacillus subtilis natto. Polyhydroxyalkanoates Levana Fermentation Cupriavidus necator Bacillus subtilis natto. |
description |
The sequential production of polyhydroxyalkanoates (PHA) and levan was investigated by microbial fermentation using agro-industrial residues. PHA production was carried out by Cupriavidus necator DSMZ 545 in two steps: a) bacterial growth, using pure (MN) and hydrolyzed (MH) molasses and; b) accumulation of PHA, using standard glycerol (GP) and crude glycerol (GB). The fermented remaining from the C. necator growth stage was separated from the PHA-containing biomass and used in the subsequent production of levan by Bacillus subtilis (natto) Takahashi. The medium containing MH+GP generated a rate of 15 mg of PHA, while the medium based on MH+GB the rate of PHA was almost twice as high (28.4 mg). FTIR spectroscopic analysis of PHA indicated stretching vibrations characteristic for a PHB-like molecule. In turn, the levan produced in this study was precipitated with different volumes of ethanol, generating levans with different molecular weights. The results of the characterization of this fructan by chromatography showed that it was predominantly constituted by fructose units. In addition, the dynamic and kinematic viscosity values for levan were similar in the analyzed concentrations and, therefore, did not provide any clues about the molecular weight of this biopolymer. Finally, the sequential production process of PHA and levan, as a second bioproduct, represents an elegant alternative to reduce the total costs of PHA production. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-03-16 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/40745 10.33448/rsd-v12i3.40745 |
url |
https://rsdjournal.org/index.php/rsd/article/view/40745 |
identifier_str_mv |
10.33448/rsd-v12i3.40745 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/40745/33242 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 Edmilson Clarindo de Siqueira; Laureen Michelle Houllou https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 Edmilson Clarindo de Siqueira; Laureen Michelle Houllou https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 12 No. 3; e25212340745 Research, Society and Development; Vol. 12 Núm. 3; e25212340745 Research, Society and Development; v. 12 n. 3; e25212340745 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
_version_ |
1797052620513214464 |