Sequential production of two biopolymers-polyhydroxyalkanoate and levan by microbial fermentation

Detalhes bibliográficos
Autor(a) principal: Siqueira, Edmilson Clarindo de
Data de Publicação: 2023
Outros Autores: Houllou , Laureen Michelle
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/40745
Resumo: The sequential production of polyhydroxyalkanoates (PHA) and levan was investigated by microbial fermentation using agro-industrial residues. PHA production was carried out by Cupriavidus necator DSMZ 545 in two steps: a) bacterial growth, using pure (MN) and hydrolyzed (MH) molasses and; b) accumulation of PHA, using standard glycerol (GP) and crude glycerol (GB). The fermented remaining from the C. necator growth stage was separated from the PHA-containing biomass and used in the subsequent production of levan by Bacillus subtilis (natto) Takahashi. The medium containing MH+GP generated a rate of 15 mg of PHA, while the medium based on MH+GB the rate of PHA was almost twice as high (28.4 mg). FTIR spectroscopic analysis of PHA indicated stretching vibrations characteristic for a PHB-like molecule. In turn, the levan produced in this study was precipitated with different volumes of ethanol, generating levans with different molecular weights. The results of the characterization of this fructan by chromatography showed that it was predominantly constituted by fructose units. In addition, the dynamic and kinematic viscosity values for levan were similar in the analyzed concentrations and, therefore, did not provide any clues about the molecular weight of this biopolymer. Finally, the sequential production process of PHA and levan, as a second bioproduct, represents an elegant alternative to reduce the total costs of PHA production.
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spelling Sequential production of two biopolymers-polyhydroxyalkanoate and levan by microbial fermentationProducción secuencial de dos biopolímeros-polihidroxialcanoato y leván por fermentación microbianaProdução sequencial de dois biopolímeros-polihidroxialcanoato e levana por fermentação microbianaPolihidroxialcanoatosLevanaFermentaçãoCupriavidus necatorBacillus subtilis natto.PolihidroxialcanoatosLevanaFermentaciónCupriavidus necatorBacillus subtilis natto.PolyhydroxyalkanoatesLevanaFermentationCupriavidus necatorBacillus subtilis natto.The sequential production of polyhydroxyalkanoates (PHA) and levan was investigated by microbial fermentation using agro-industrial residues. PHA production was carried out by Cupriavidus necator DSMZ 545 in two steps: a) bacterial growth, using pure (MN) and hydrolyzed (MH) molasses and; b) accumulation of PHA, using standard glycerol (GP) and crude glycerol (GB). The fermented remaining from the C. necator growth stage was separated from the PHA-containing biomass and used in the subsequent production of levan by Bacillus subtilis (natto) Takahashi. The medium containing MH+GP generated a rate of 15 mg of PHA, while the medium based on MH+GB the rate of PHA was almost twice as high (28.4 mg). FTIR spectroscopic analysis of PHA indicated stretching vibrations characteristic for a PHB-like molecule. In turn, the levan produced in this study was precipitated with different volumes of ethanol, generating levans with different molecular weights. The results of the characterization of this fructan by chromatography showed that it was predominantly constituted by fructose units. In addition, the dynamic and kinematic viscosity values for levan were similar in the analyzed concentrations and, therefore, did not provide any clues about the molecular weight of this biopolymer. Finally, the sequential production process of PHA and levan, as a second bioproduct, represents an elegant alternative to reduce the total costs of PHA production.Se investigó la producción secuencial de polihidroxialcanoatos (PHA) y leván mediante fermentación microbiana utilizando residuos agroindustriales. La producción de PHA fue realizada por Cupriavidus necator DSMZ 545 en dos pasos: a) crecimiento bacteriano, utilizando melaza pura (MN) e hidrolizada (MH) y; b) acumulación de PHA, utilizando glicerol estándar (GP) y glicerol crudo (GB). El resto fermentado de la etapa de crecimiento de C. necator se separó de la biomasa que contenía PHA y se usó en la producción posterior de levan por Bacillus subtilis (natto) Takahashi. El medio que contenía MH+GP generó una tasa de 15 mg de PHA, mientras que el medio basado en MH+GB la tasa de PHA fue casi el doble (28,4 mg). El análisis espectroscópico FTIR de PHA indicó vibraciones de estiramiento características de una molécula similar a PHB. A su vez, el leván producido en este estudio se precipitó con diferentes volúmenes de etanol, generando leván con diferentes pesos moleculares. Los resultados de la caracterización de este fructano por cromatografía mostraron que estaba predominantemente constituido por unidades de fructosa. Además, los valores de viscosidad dinámica y cinemática del leván fueron similares en las concentraciones analizadas y, por tanto, no aportaron pistas sobre el peso molecular de este biopolímero. Finalmente, el proceso de producción secuencial de PHA y leván, como segundo bioproducto, representa una alternativa elegante para reducir los costos totales de producción de PHA.A produção sequencial de polihidroxialcanoatos (PHA) e levana foi investigada por fermentação microbiana usando resíduos agroindustriais. A produção de PHA foi realizada por Cupriavidus necator DSMZ 545 em duas etapas: a) crescimento bacteriano, usando melaço puro (MN) e hidrolisado (MH) e; b) acumulação de PHA, usando glicerol padrão (GP) e glicerol bruto (GB). O fermentado remanescente da etapa de crescimento de C. necator foi separado da biomassa contendo PHA e usado na produção subsequente de levana por Bacillus subtilis (natto) Takahashi. O meio contendo MH+GP gerou uma taxa de 15 mg de PHA, enquanto o meio à base de MH+GB a taxa de PHA foi quase duas vezes maior (28,4 mg). A análise espectroscópica por FTIR de PHA indicou vibrações de estiramentos característicos para uma molécula do tipo PHB. Por sua vez, levana produzida neste estudo foi precipitada com diferentes volumes de etanol, gerando levanas com diferentes pesos moleculares. Os resultados da caracterização desta frutana por cromatografia mostrou que a mesma era constituída predominantemente por unidades de frutose. Além disso, os valores de viscosidade dinâmica e cinemática para a levana foram semelhantes nas concentrações analisadas e, portanto, não forneceram indícios acerca do peso molecular desse biopolímero. Por fim, o processo de produção sequencial de PHA e levana, como um segundo bioproduto, representa uma alternativa elegante para reduzir os custos totais de produção do PHA.Research, Society and Development2023-03-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/4074510.33448/rsd-v12i3.40745Research, Society and Development; Vol. 12 No. 3; e25212340745Research, Society and Development; Vol. 12 Núm. 3; e25212340745Research, Society and Development; v. 12 n. 3; e252123407452525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/40745/33242Copyright (c) 2023 Edmilson Clarindo de Siqueira; Laureen Michelle Houllou https://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSiqueira, Edmilson Clarindo de Houllou , Laureen Michelle 2023-03-23T08:33:38Zoai:ojs.pkp.sfu.ca:article/40745Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2023-03-23T08:33:38Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Sequential production of two biopolymers-polyhydroxyalkanoate and levan by microbial fermentation
Producción secuencial de dos biopolímeros-polihidroxialcanoato y leván por fermentación microbiana
Produção sequencial de dois biopolímeros-polihidroxialcanoato e levana por fermentação microbiana
title Sequential production of two biopolymers-polyhydroxyalkanoate and levan by microbial fermentation
spellingShingle Sequential production of two biopolymers-polyhydroxyalkanoate and levan by microbial fermentation
Siqueira, Edmilson Clarindo de
Polihidroxialcanoatos
Levana
Fermentação
Cupriavidus necator
Bacillus subtilis natto.
Polihidroxialcanoatos
Levana
Fermentación
Cupriavidus necator
Bacillus subtilis natto.
Polyhydroxyalkanoates
Levana
Fermentation
Cupriavidus necator
Bacillus subtilis natto.
title_short Sequential production of two biopolymers-polyhydroxyalkanoate and levan by microbial fermentation
title_full Sequential production of two biopolymers-polyhydroxyalkanoate and levan by microbial fermentation
title_fullStr Sequential production of two biopolymers-polyhydroxyalkanoate and levan by microbial fermentation
title_full_unstemmed Sequential production of two biopolymers-polyhydroxyalkanoate and levan by microbial fermentation
title_sort Sequential production of two biopolymers-polyhydroxyalkanoate and levan by microbial fermentation
author Siqueira, Edmilson Clarindo de
author_facet Siqueira, Edmilson Clarindo de
Houllou , Laureen Michelle
author_role author
author2 Houllou , Laureen Michelle
author2_role author
dc.contributor.author.fl_str_mv Siqueira, Edmilson Clarindo de
Houllou , Laureen Michelle
dc.subject.por.fl_str_mv Polihidroxialcanoatos
Levana
Fermentação
Cupriavidus necator
Bacillus subtilis natto.
Polihidroxialcanoatos
Levana
Fermentación
Cupriavidus necator
Bacillus subtilis natto.
Polyhydroxyalkanoates
Levana
Fermentation
Cupriavidus necator
Bacillus subtilis natto.
topic Polihidroxialcanoatos
Levana
Fermentação
Cupriavidus necator
Bacillus subtilis natto.
Polihidroxialcanoatos
Levana
Fermentación
Cupriavidus necator
Bacillus subtilis natto.
Polyhydroxyalkanoates
Levana
Fermentation
Cupriavidus necator
Bacillus subtilis natto.
description The sequential production of polyhydroxyalkanoates (PHA) and levan was investigated by microbial fermentation using agro-industrial residues. PHA production was carried out by Cupriavidus necator DSMZ 545 in two steps: a) bacterial growth, using pure (MN) and hydrolyzed (MH) molasses and; b) accumulation of PHA, using standard glycerol (GP) and crude glycerol (GB). The fermented remaining from the C. necator growth stage was separated from the PHA-containing biomass and used in the subsequent production of levan by Bacillus subtilis (natto) Takahashi. The medium containing MH+GP generated a rate of 15 mg of PHA, while the medium based on MH+GB the rate of PHA was almost twice as high (28.4 mg). FTIR spectroscopic analysis of PHA indicated stretching vibrations characteristic for a PHB-like molecule. In turn, the levan produced in this study was precipitated with different volumes of ethanol, generating levans with different molecular weights. The results of the characterization of this fructan by chromatography showed that it was predominantly constituted by fructose units. In addition, the dynamic and kinematic viscosity values for levan were similar in the analyzed concentrations and, therefore, did not provide any clues about the molecular weight of this biopolymer. Finally, the sequential production process of PHA and levan, as a second bioproduct, represents an elegant alternative to reduce the total costs of PHA production.
publishDate 2023
dc.date.none.fl_str_mv 2023-03-16
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/40745
10.33448/rsd-v12i3.40745
url https://rsdjournal.org/index.php/rsd/article/view/40745
identifier_str_mv 10.33448/rsd-v12i3.40745
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/40745/33242
dc.rights.driver.fl_str_mv Copyright (c) 2023 Edmilson Clarindo de Siqueira; Laureen Michelle Houllou
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2023 Edmilson Clarindo de Siqueira; Laureen Michelle Houllou
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 12 No. 3; e25212340745
Research, Society and Development; Vol. 12 Núm. 3; e25212340745
Research, Society and Development; v. 12 n. 3; e25212340745
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052620513214464

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