Microbiological analysis of dental equipment water

Detalhes bibliográficos
Autor(a) principal: Amancio, Amanda de Medeiros
Data de Publicação: 2020
Outros Autores: Silva, Bianca Cristina Dantas da, Viana, Jaiane Carmélia Monteiro, Avelino, Luanny de Brito, Sousa, Lucas Cavalcante de, Lima, Kênio Costa, Macêdo-Costa, Maria Regina
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/6818
Resumo: In the last years, the relevance of sterilization and antisepsis processes has been widely discussed among healthcare professionals, including dental surgeons. However, in dental practice, the quality of the water used in the equipments, apparently harmless, can be considered a risk factor for the patient's health. Therefore, the objective of this study was to verify the presence of contamination in dental equipments and to evaluate the water quality of the equipment's reservoirs, through microbiological analysis. 12 water samples were collected directly from dental chair reservoirs and 12 samples from the triple syringe of six multidisciplinary clinics in the Department of Dentistry of the Federal University of Rio Grande do Norte (UFRN). Plating was performed using the Spread Plate method, on BHI agar medium (Brain Heart Infusion) with subsequent incubation in a bacteriological oven programmed at 37,5°C to 38°C, for a period of 24 to 48 hours. The results demonstrated that 83% of the incubated plates showed significant growth of filamentous fungi and bacteria that, due to the odor of tutti-fruti and based on macroscopic characteristics, are suggested to be of the genus Pseudomonas. In this context, the findings of this study attest to the urgency of developing a health care routine, enabling the decontamination of the equipment's water systems as well as its reservoirs, meeting patient safety requirements, which must be inherent in dental offices.
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spelling Microbiological analysis of dental equipment waterAnálisis microbiológico del agua del equipo dentalAnálise microbiológica da água de equipamentos odontológicosWaterDental officesMicrobiological analysisContamination.ÁguaConsultórios odontológicosAnálise microbiológicaContaminação.AguaConsultorios dentalesAnálisis microbiológicoContaminación.In the last years, the relevance of sterilization and antisepsis processes has been widely discussed among healthcare professionals, including dental surgeons. However, in dental practice, the quality of the water used in the equipments, apparently harmless, can be considered a risk factor for the patient's health. Therefore, the objective of this study was to verify the presence of contamination in dental equipments and to evaluate the water quality of the equipment's reservoirs, through microbiological analysis. 12 water samples were collected directly from dental chair reservoirs and 12 samples from the triple syringe of six multidisciplinary clinics in the Department of Dentistry of the Federal University of Rio Grande do Norte (UFRN). Plating was performed using the Spread Plate method, on BHI agar medium (Brain Heart Infusion) with subsequent incubation in a bacteriological oven programmed at 37,5°C to 38°C, for a period of 24 to 48 hours. The results demonstrated that 83% of the incubated plates showed significant growth of filamentous fungi and bacteria that, due to the odor of tutti-fruti and based on macroscopic characteristics, are suggested to be of the genus Pseudomonas. In this context, the findings of this study attest to the urgency of developing a health care routine, enabling the decontamination of the equipment's water systems as well as its reservoirs, meeting patient safety requirements, which must be inherent in dental offices.En los últimos años, la relevancia de los procesos de esterilización y antisepsia ha sido ampliamente discutida entre los profesionales de la salud, incluidos los dentistas. Sin embargo, en la práctica dental, la calidad del agua utilizada en el equipo, aparentemente inofensiva, puede considerarse un factor de riesgo para la salud del paciente. Por lo tanto, el objetivo de este estudio fue verificar la presencia de contaminación en el equipo dental y evaluar la calidad del agua de los depósitos del equipo, mediante análisis microbiológicos. Se recogieron 12 muestras de agua directamente de los depósitos del sillón dental y 12 muestras de la jeringa triple de seis clínicas multidisciplinarias en el Departamento de Odontología de la Universidad Federal de Rio Grande do Norte (UFRN). El enchapado se realizó utilizando el método Spread Plate, en medio de agar BHI (Brain Heart Infusion) con incubación posterior en un horno bacteriológico programado de 37,5°C a 38°C, durante un período de 24 a 48 horas. Los resultados mostraron que el 83% de las placas incubadas mostraron un crecimiento significativo de hongos filamentosos y bacterias que, debido al olor de tutti-frutti y según las características macroscópicas, se sugiere que son del género Pseudomonas. En este contexto, los hallazgos de este estudio dan fe de la urgencia de desarrollar una rutina de atención médica, que permita la descontaminación de los sistemas de agua del equipo, así como sus depósitos, cumpliendo con los requisitos de seguridad del paciente, que deben ser inherentes a los consultorios dentales.Nos últimos anos, a relevância dos processos de esterilização e antissepsia tem sido bastante discutida entre os profissionais da área de saúde, incluindo cirurgiões-dentistas. No entanto, na prática odontológica, a qualidade da água utilizada nos equipamentos, aparentemente inofensiva, pode ser considerada um fator de risco para a saúde do paciente. Diante disso, o objetivo deste estudo foi verificar a presença de contaminação em equipamentos odontológicos e avaliar a qualidade da água dos reservatórios desses equipamentos, por meio de análise microbiológica. Foram coletadas 12 amostras de água diretamente dos reservatórios das cadeiras odontológicas e 12 amostras da seringa tríplice de seis clínicas multidisciplinares do Departamento de Odontologia da Universidade Federal do Rio Grande do Norte (UFRN). O plaqueamento foi realizado com base no método Spread Plate, em meio ágar BHI (Brain Heart Infusion) com incubação subsequente em estufa bacteriológica programada de 37,5°C a 38°C, por um período de 24 a 48 horas. Os resultados demonstraram que 83% das placas incubadas apresentaram crescimento expressivo de fungos filamentosos e bactérias que, devido ao odor de tutti-frutti e com base nas características macroscópicas, sugere-se que sejam do gênero Pseudomonas. Nesse contexto, os achados deste estudo atestam a urgência da elaboração de uma rotina de cuidados sanitários, viabilizando a descontaminação dos sistemas de água dos equipamentos bem como de seus reservatórios, atendendo aos requisitos de segurança do paciente, que deve ser inerente aos consultórios odontológicos.Research, Society and Development2020-08-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/681810.33448/rsd-v9i9.6818Research, Society and Development; Vol. 9 No. 9; e23996818Research, Society and Development; Vol. 9 Núm. 9; e23996818Research, Society and Development; v. 9 n. 9; e239968182525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/6818/6142Copyright (c) 2020 Amanda de Medeiros Amancio, Bianca Cristina Dantas da Silva, Jaiane Carmélia Monteiro Viana, Luanny de Brito Avelino, Lucas Cavalcante de Sousa, Kênio Costa Lima, Maria Regina Macêdo-Costahttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessAmancio, Amanda de MedeirosSilva, Bianca Cristina Dantas daViana, Jaiane Carmélia MonteiroAvelino, Luanny de BritoSousa, Lucas Cavalcante deLima, Kênio CostaMacêdo-Costa, Maria Regina2020-09-18T01:42:11Zoai:ojs.pkp.sfu.ca:article/6818Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:29:47.620721Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Microbiological analysis of dental equipment water
Análisis microbiológico del agua del equipo dental
Análise microbiológica da água de equipamentos odontológicos
title Microbiological analysis of dental equipment water
spellingShingle Microbiological analysis of dental equipment water
Amancio, Amanda de Medeiros
Water
Dental offices
Microbiological analysis
Contamination.
Água
Consultórios odontológicos
Análise microbiológica
Contaminação.
Agua
Consultorios dentales
Análisis microbiológico
Contaminación.
title_short Microbiological analysis of dental equipment water
title_full Microbiological analysis of dental equipment water
title_fullStr Microbiological analysis of dental equipment water
title_full_unstemmed Microbiological analysis of dental equipment water
title_sort Microbiological analysis of dental equipment water
author Amancio, Amanda de Medeiros
author_facet Amancio, Amanda de Medeiros
Silva, Bianca Cristina Dantas da
Viana, Jaiane Carmélia Monteiro
Avelino, Luanny de Brito
Sousa, Lucas Cavalcante de
Lima, Kênio Costa
Macêdo-Costa, Maria Regina
author_role author
author2 Silva, Bianca Cristina Dantas da
Viana, Jaiane Carmélia Monteiro
Avelino, Luanny de Brito
Sousa, Lucas Cavalcante de
Lima, Kênio Costa
Macêdo-Costa, Maria Regina
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Amancio, Amanda de Medeiros
Silva, Bianca Cristina Dantas da
Viana, Jaiane Carmélia Monteiro
Avelino, Luanny de Brito
Sousa, Lucas Cavalcante de
Lima, Kênio Costa
Macêdo-Costa, Maria Regina
dc.subject.por.fl_str_mv Water
Dental offices
Microbiological analysis
Contamination.
Água
Consultórios odontológicos
Análise microbiológica
Contaminação.
Agua
Consultorios dentales
Análisis microbiológico
Contaminación.
topic Water
Dental offices
Microbiological analysis
Contamination.
Água
Consultórios odontológicos
Análise microbiológica
Contaminação.
Agua
Consultorios dentales
Análisis microbiológico
Contaminación.
description In the last years, the relevance of sterilization and antisepsis processes has been widely discussed among healthcare professionals, including dental surgeons. However, in dental practice, the quality of the water used in the equipments, apparently harmless, can be considered a risk factor for the patient's health. Therefore, the objective of this study was to verify the presence of contamination in dental equipments and to evaluate the water quality of the equipment's reservoirs, through microbiological analysis. 12 water samples were collected directly from dental chair reservoirs and 12 samples from the triple syringe of six multidisciplinary clinics in the Department of Dentistry of the Federal University of Rio Grande do Norte (UFRN). Plating was performed using the Spread Plate method, on BHI agar medium (Brain Heart Infusion) with subsequent incubation in a bacteriological oven programmed at 37,5°C to 38°C, for a period of 24 to 48 hours. The results demonstrated that 83% of the incubated plates showed significant growth of filamentous fungi and bacteria that, due to the odor of tutti-fruti and based on macroscopic characteristics, are suggested to be of the genus Pseudomonas. In this context, the findings of this study attest to the urgency of developing a health care routine, enabling the decontamination of the equipment's water systems as well as its reservoirs, meeting patient safety requirements, which must be inherent in dental offices.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-09
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/6818
10.33448/rsd-v9i9.6818
url https://rsdjournal.org/index.php/rsd/article/view/6818
identifier_str_mv 10.33448/rsd-v9i9.6818
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/6818/6142
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 9 No. 9; e23996818
Research, Society and Development; Vol. 9 Núm. 9; e23996818
Research, Society and Development; v. 9 n. 9; e23996818
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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