Microbiological analysis of dental equipment water
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/6818 |
Resumo: | In the last years, the relevance of sterilization and antisepsis processes has been widely discussed among healthcare professionals, including dental surgeons. However, in dental practice, the quality of the water used in the equipments, apparently harmless, can be considered a risk factor for the patient's health. Therefore, the objective of this study was to verify the presence of contamination in dental equipments and to evaluate the water quality of the equipment's reservoirs, through microbiological analysis. 12 water samples were collected directly from dental chair reservoirs and 12 samples from the triple syringe of six multidisciplinary clinics in the Department of Dentistry of the Federal University of Rio Grande do Norte (UFRN). Plating was performed using the Spread Plate method, on BHI agar medium (Brain Heart Infusion) with subsequent incubation in a bacteriological oven programmed at 37,5°C to 38°C, for a period of 24 to 48 hours. The results demonstrated that 83% of the incubated plates showed significant growth of filamentous fungi and bacteria that, due to the odor of tutti-fruti and based on macroscopic characteristics, are suggested to be of the genus Pseudomonas. In this context, the findings of this study attest to the urgency of developing a health care routine, enabling the decontamination of the equipment's water systems as well as its reservoirs, meeting patient safety requirements, which must be inherent in dental offices. |
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Microbiological analysis of dental equipment waterAnálisis microbiológico del agua del equipo dentalAnálise microbiológica da água de equipamentos odontológicosWaterDental officesMicrobiological analysisContamination.ÁguaConsultórios odontológicosAnálise microbiológicaContaminação.AguaConsultorios dentalesAnálisis microbiológicoContaminación.In the last years, the relevance of sterilization and antisepsis processes has been widely discussed among healthcare professionals, including dental surgeons. However, in dental practice, the quality of the water used in the equipments, apparently harmless, can be considered a risk factor for the patient's health. Therefore, the objective of this study was to verify the presence of contamination in dental equipments and to evaluate the water quality of the equipment's reservoirs, through microbiological analysis. 12 water samples were collected directly from dental chair reservoirs and 12 samples from the triple syringe of six multidisciplinary clinics in the Department of Dentistry of the Federal University of Rio Grande do Norte (UFRN). Plating was performed using the Spread Plate method, on BHI agar medium (Brain Heart Infusion) with subsequent incubation in a bacteriological oven programmed at 37,5°C to 38°C, for a period of 24 to 48 hours. The results demonstrated that 83% of the incubated plates showed significant growth of filamentous fungi and bacteria that, due to the odor of tutti-fruti and based on macroscopic characteristics, are suggested to be of the genus Pseudomonas. In this context, the findings of this study attest to the urgency of developing a health care routine, enabling the decontamination of the equipment's water systems as well as its reservoirs, meeting patient safety requirements, which must be inherent in dental offices.En los últimos años, la relevancia de los procesos de esterilización y antisepsia ha sido ampliamente discutida entre los profesionales de la salud, incluidos los dentistas. Sin embargo, en la práctica dental, la calidad del agua utilizada en el equipo, aparentemente inofensiva, puede considerarse un factor de riesgo para la salud del paciente. Por lo tanto, el objetivo de este estudio fue verificar la presencia de contaminación en el equipo dental y evaluar la calidad del agua de los depósitos del equipo, mediante análisis microbiológicos. Se recogieron 12 muestras de agua directamente de los depósitos del sillón dental y 12 muestras de la jeringa triple de seis clínicas multidisciplinarias en el Departamento de Odontología de la Universidad Federal de Rio Grande do Norte (UFRN). El enchapado se realizó utilizando el método Spread Plate, en medio de agar BHI (Brain Heart Infusion) con incubación posterior en un horno bacteriológico programado de 37,5°C a 38°C, durante un período de 24 a 48 horas. Los resultados mostraron que el 83% de las placas incubadas mostraron un crecimiento significativo de hongos filamentosos y bacterias que, debido al olor de tutti-frutti y según las características macroscópicas, se sugiere que son del género Pseudomonas. En este contexto, los hallazgos de este estudio dan fe de la urgencia de desarrollar una rutina de atención médica, que permita la descontaminación de los sistemas de agua del equipo, así como sus depósitos, cumpliendo con los requisitos de seguridad del paciente, que deben ser inherentes a los consultorios dentales.Nos últimos anos, a relevância dos processos de esterilização e antissepsia tem sido bastante discutida entre os profissionais da área de saúde, incluindo cirurgiões-dentistas. No entanto, na prática odontológica, a qualidade da água utilizada nos equipamentos, aparentemente inofensiva, pode ser considerada um fator de risco para a saúde do paciente. Diante disso, o objetivo deste estudo foi verificar a presença de contaminação em equipamentos odontológicos e avaliar a qualidade da água dos reservatórios desses equipamentos, por meio de análise microbiológica. Foram coletadas 12 amostras de água diretamente dos reservatórios das cadeiras odontológicas e 12 amostras da seringa tríplice de seis clínicas multidisciplinares do Departamento de Odontologia da Universidade Federal do Rio Grande do Norte (UFRN). O plaqueamento foi realizado com base no método Spread Plate, em meio ágar BHI (Brain Heart Infusion) com incubação subsequente em estufa bacteriológica programada de 37,5°C a 38°C, por um período de 24 a 48 horas. Os resultados demonstraram que 83% das placas incubadas apresentaram crescimento expressivo de fungos filamentosos e bactérias que, devido ao odor de tutti-frutti e com base nas características macroscópicas, sugere-se que sejam do gênero Pseudomonas. Nesse contexto, os achados deste estudo atestam a urgência da elaboração de uma rotina de cuidados sanitários, viabilizando a descontaminação dos sistemas de água dos equipamentos bem como de seus reservatórios, atendendo aos requisitos de segurança do paciente, que deve ser inerente aos consultórios odontológicos.Research, Society and Development2020-08-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/681810.33448/rsd-v9i9.6818Research, Society and Development; Vol. 9 No. 9; e23996818Research, Society and Development; Vol. 9 Núm. 9; e23996818Research, Society and Development; v. 9 n. 9; e239968182525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/6818/6142Copyright (c) 2020 Amanda de Medeiros Amancio, Bianca Cristina Dantas da Silva, Jaiane Carmélia Monteiro Viana, Luanny de Brito Avelino, Lucas Cavalcante de Sousa, Kênio Costa Lima, Maria Regina Macêdo-Costahttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessAmancio, Amanda de MedeirosSilva, Bianca Cristina Dantas daViana, Jaiane Carmélia MonteiroAvelino, Luanny de BritoSousa, Lucas Cavalcante deLima, Kênio CostaMacêdo-Costa, Maria Regina2020-09-18T01:42:11Zoai:ojs.pkp.sfu.ca:article/6818Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:29:47.620721Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Microbiological analysis of dental equipment water Análisis microbiológico del agua del equipo dental Análise microbiológica da água de equipamentos odontológicos |
title |
Microbiological analysis of dental equipment water |
spellingShingle |
Microbiological analysis of dental equipment water Amancio, Amanda de Medeiros Water Dental offices Microbiological analysis Contamination. Água Consultórios odontológicos Análise microbiológica Contaminação. Agua Consultorios dentales Análisis microbiológico Contaminación. |
title_short |
Microbiological analysis of dental equipment water |
title_full |
Microbiological analysis of dental equipment water |
title_fullStr |
Microbiological analysis of dental equipment water |
title_full_unstemmed |
Microbiological analysis of dental equipment water |
title_sort |
Microbiological analysis of dental equipment water |
author |
Amancio, Amanda de Medeiros |
author_facet |
Amancio, Amanda de Medeiros Silva, Bianca Cristina Dantas da Viana, Jaiane Carmélia Monteiro Avelino, Luanny de Brito Sousa, Lucas Cavalcante de Lima, Kênio Costa Macêdo-Costa, Maria Regina |
author_role |
author |
author2 |
Silva, Bianca Cristina Dantas da Viana, Jaiane Carmélia Monteiro Avelino, Luanny de Brito Sousa, Lucas Cavalcante de Lima, Kênio Costa Macêdo-Costa, Maria Regina |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Amancio, Amanda de Medeiros Silva, Bianca Cristina Dantas da Viana, Jaiane Carmélia Monteiro Avelino, Luanny de Brito Sousa, Lucas Cavalcante de Lima, Kênio Costa Macêdo-Costa, Maria Regina |
dc.subject.por.fl_str_mv |
Water Dental offices Microbiological analysis Contamination. Água Consultórios odontológicos Análise microbiológica Contaminação. Agua Consultorios dentales Análisis microbiológico Contaminación. |
topic |
Water Dental offices Microbiological analysis Contamination. Água Consultórios odontológicos Análise microbiológica Contaminação. Agua Consultorios dentales Análisis microbiológico Contaminación. |
description |
In the last years, the relevance of sterilization and antisepsis processes has been widely discussed among healthcare professionals, including dental surgeons. However, in dental practice, the quality of the water used in the equipments, apparently harmless, can be considered a risk factor for the patient's health. Therefore, the objective of this study was to verify the presence of contamination in dental equipments and to evaluate the water quality of the equipment's reservoirs, through microbiological analysis. 12 water samples were collected directly from dental chair reservoirs and 12 samples from the triple syringe of six multidisciplinary clinics in the Department of Dentistry of the Federal University of Rio Grande do Norte (UFRN). Plating was performed using the Spread Plate method, on BHI agar medium (Brain Heart Infusion) with subsequent incubation in a bacteriological oven programmed at 37,5°C to 38°C, for a period of 24 to 48 hours. The results demonstrated that 83% of the incubated plates showed significant growth of filamentous fungi and bacteria that, due to the odor of tutti-fruti and based on macroscopic characteristics, are suggested to be of the genus Pseudomonas. In this context, the findings of this study attest to the urgency of developing a health care routine, enabling the decontamination of the equipment's water systems as well as its reservoirs, meeting patient safety requirements, which must be inherent in dental offices. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-08-09 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/6818 10.33448/rsd-v9i9.6818 |
url |
https://rsdjournal.org/index.php/rsd/article/view/6818 |
identifier_str_mv |
10.33448/rsd-v9i9.6818 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/6818/6142 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 9 No. 9; e23996818 Research, Society and Development; Vol. 9 Núm. 9; e23996818 Research, Society and Development; v. 9 n. 9; e23996818 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052655459106816 |