Polymorphisms in the IL17A gene do not influence the clinical, laboratory and radiological manifestations of tuberculosis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Research, Society and Development |
| Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/33518 |
Resumo: | Some single-base polymorphisms (SNPs) in cytokine genes have the potential to affect gene expression and/or biological activity of the respective cytokine, and there is evidence of the association of different SNPs with tuberculosis in several different populations. Studies involving the influence of SNPs on the IL17A gene are still controversial, behaving differently among different populations. Thus, research into the association of SNPs in the IL17A gene with the clinical course of the disease may contribute to a better understanding of TB susceptibility and development. Therefore, the aim of the present study was to evaluate the influence of SNPs on the IL17A gene and their clinical-laboratory association in patients with tuberculosis. Therefore, 35 patients with tuberculosis and 26 controls were studied. The evaluation of the SNPs in the IL17A gene, rs7747909 and rs2275913, was performed using the allelic discrimination technique by real-time PCR. Clinical, laboratory and radiological data were obtained by collecting medical records. Analyzes were performed using Fisher's test, considering significant values p<0.05. There was no association of SNPs in the IL17A gene evaluated, either in relation to susceptibility or in terms of clinical, laboratory and radiological association. Therefore, it is essential to advance in research to analyze the influence of other SNPs on the IL17A gene and the relationship with other variables linked to the immune response to M. tuberculosis. |
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Polymorphisms in the IL17A gene do not influence the clinical, laboratory and radiological manifestations of tuberculosisLos polimorfismos en el gen IL17A no influyen en las manifestaciones clínicas, de laboratorio y radiológicas de la tuberculosisPolimorfismos no gene IL17A não influenciam nas manifestações clínicas, laboratoriais e radiológicas da tuberculoseCytokinePolymorphism, GeneticTuberculosis.CitocinaPolimorfismo genéticoTuberculosis.CitocinaPolimorfismo genéticoTuberculose.Some single-base polymorphisms (SNPs) in cytokine genes have the potential to affect gene expression and/or biological activity of the respective cytokine, and there is evidence of the association of different SNPs with tuberculosis in several different populations. Studies involving the influence of SNPs on the IL17A gene are still controversial, behaving differently among different populations. Thus, research into the association of SNPs in the IL17A gene with the clinical course of the disease may contribute to a better understanding of TB susceptibility and development. Therefore, the aim of the present study was to evaluate the influence of SNPs on the IL17A gene and their clinical-laboratory association in patients with tuberculosis. Therefore, 35 patients with tuberculosis and 26 controls were studied. The evaluation of the SNPs in the IL17A gene, rs7747909 and rs2275913, was performed using the allelic discrimination technique by real-time PCR. Clinical, laboratory and radiological data were obtained by collecting medical records. Analyzes were performed using Fisher's test, considering significant values p<0.05. There was no association of SNPs in the IL17A gene evaluated, either in relation to susceptibility or in terms of clinical, laboratory and radiological association. Therefore, it is essential to advance in research to analyze the influence of other SNPs on the IL17A gene and the relationship with other variables linked to the immune response to M. tuberculosis.Algunos polimorfismos de base única (SNP) en genes de citoquinas tienen el potencial de afectar la expresión génica y/o la actividad biológica de la citoquina respectiva, y hay evidencia de la asociación de diferentes SNP con tuberculosis en varias poblaciones diferentes. Los estudios que involucran la influencia de los SNP en el gen IL17A aún son controvertidos y se comportan de manera diferente entre diferentes poblaciones. Por lo tanto, la investigación sobre la asociación de SNP en el gen IL17A con el curso clínico de la enfermedad puede contribuir a una mejor comprensión de la susceptibilidad y el desarrollo de la TB. Por tanto, el objetivo del presente estudio fue evaluar la influencia de los SNPs en el gen IL17A y su asociación clínico-laboratorio en pacientes con tuberculosis. Por tanto, se estudiaron 35 pacientes con tuberculosis y 26 controles. La evaluación de los SNPs en el gen IL17A, rs7747909 y rs2275913, se realizó mediante la técnica de discriminación alélica por PCR en tiempo real. Los datos clínicos, de laboratorio y radiológicos se obtuvieron mediante la recopilación de historias clínicas. Los análisis se realizaron mediante la prueba de Fisher, considerando valores significativos p<0,05. No hubo asociación de SNPs en el gen IL17A evaluado, ni en relación a la susceptibilidad ni en términos de asociación clínicos, de laboratorio y radiológicos. Por ello, es fundamental avanzar en la investigación para analizar la influencia de otros SNPs sobre el gen IL17A y la relación con otras variables ligadas a la respuesta inmune frente a M. tuberculosis.Alguns polimorfismos de base única (SNP) nos genes das citocinas apresentam potencialidade para afetar a expressão gênica e/ou a atividade biológica da respectiva citocina, e existem evidências da associação de diferentes SNPs com a tuberculose em diversas populações diferentes. Os estudos envolvendo a influência de SNPs no gene da IL17A ainda são controversos, comportando-se de forma variada entre diferentes populações. Desta forma, a pesquisa da associação de SNPs no gene da IL17A com a evolução clínica da doença, poderá contribuir para um melhor entendimento da susceptibilidade e o desenvolvimento da TB. Para tanto, o objetivo do presente estudo foi avaliar a influência de SNPs no gene IL17A e a sua associação clínico-laboratorial em pacientes com tuberculose. Para tanto, foram estudados 35 pacientes com tuberculose e 26 controles. A avaliação dos SNPs no gene IL17A, rs7747909 e rs2275913, foi realizada através da técnica de discriminação alélica por PCR em tempo real. Os dados clínicos, laboratoriais e radiológicos foram obtidos através da coleta de prontuário. As análises foram realizadas pelo teste de Fisher, considerando valores significativos p<0,05. Não houve associação dos SNPs no gene IL17A avaliados, seja em relação à susceptibilidade ou quanto à associção clínica, laboratorial e radiológica. Por conseguinte, é fundamental o avanço em pesquisas para analisar a influência de outros SNPs no gene da IL17A e a relação com outras variáveis ligadas à resposta imune ao M. tuberculosis.Research, Society and Development2022-08-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/3351810.33448/rsd-v11i11.33518Research, Society and Development; Vol. 11 No. 11; e95111133518Research, Society and Development; Vol. 11 Núm. 11; e95111133518Research, Society and Development; v. 11 n. 11; e951111335182525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/33518/28232Copyright (c) 2022 Suelen Aparecida Felício; Ualter Guilherme Cipriano Rosa; João Guilherme Araujo Matarazo; Amanda Aparecida Silva de Aguiar; Paulo José Mascarenhas Mazaro; Elaine Cristina Negri Santos; Eliana Peresi-Lordelohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessFelício, Suelen Aparecida Rosa, Ualter Guilherme Cipriano Matarazo, João Guilherme Araujo Aguiar, Amanda Aparecida Silva deMazaro, Paulo José Mascarenhas Santos, Elaine Cristina Negri Peresi-Lordelo, Eliana2022-09-05T13:24:46Zoai:ojs.pkp.sfu.ca:article/33518Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:49:08.364627Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
| dc.title.none.fl_str_mv |
Polymorphisms in the IL17A gene do not influence the clinical, laboratory and radiological manifestations of tuberculosis Los polimorfismos en el gen IL17A no influyen en las manifestaciones clínicas, de laboratorio y radiológicas de la tuberculosis Polimorfismos no gene IL17A não influenciam nas manifestações clínicas, laboratoriais e radiológicas da tuberculose |
| title |
Polymorphisms in the IL17A gene do not influence the clinical, laboratory and radiological manifestations of tuberculosis |
| spellingShingle |
Polymorphisms in the IL17A gene do not influence the clinical, laboratory and radiological manifestations of tuberculosis Felício, Suelen Aparecida Cytokine Polymorphism, Genetic Tuberculosis. Citocina Polimorfismo genético Tuberculosis. Citocina Polimorfismo genético Tuberculose. |
| title_short |
Polymorphisms in the IL17A gene do not influence the clinical, laboratory and radiological manifestations of tuberculosis |
| title_full |
Polymorphisms in the IL17A gene do not influence the clinical, laboratory and radiological manifestations of tuberculosis |
| title_fullStr |
Polymorphisms in the IL17A gene do not influence the clinical, laboratory and radiological manifestations of tuberculosis |
| title_full_unstemmed |
Polymorphisms in the IL17A gene do not influence the clinical, laboratory and radiological manifestations of tuberculosis |
| title_sort |
Polymorphisms in the IL17A gene do not influence the clinical, laboratory and radiological manifestations of tuberculosis |
| author |
Felício, Suelen Aparecida |
| author_facet |
Felício, Suelen Aparecida Rosa, Ualter Guilherme Cipriano Matarazo, João Guilherme Araujo Aguiar, Amanda Aparecida Silva de Mazaro, Paulo José Mascarenhas Santos, Elaine Cristina Negri Peresi-Lordelo, Eliana |
| author_role |
author |
| author2 |
Rosa, Ualter Guilherme Cipriano Matarazo, João Guilherme Araujo Aguiar, Amanda Aparecida Silva de Mazaro, Paulo José Mascarenhas Santos, Elaine Cristina Negri Peresi-Lordelo, Eliana |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Felício, Suelen Aparecida Rosa, Ualter Guilherme Cipriano Matarazo, João Guilherme Araujo Aguiar, Amanda Aparecida Silva de Mazaro, Paulo José Mascarenhas Santos, Elaine Cristina Negri Peresi-Lordelo, Eliana |
| dc.subject.por.fl_str_mv |
Cytokine Polymorphism, Genetic Tuberculosis. Citocina Polimorfismo genético Tuberculosis. Citocina Polimorfismo genético Tuberculose. |
| topic |
Cytokine Polymorphism, Genetic Tuberculosis. Citocina Polimorfismo genético Tuberculosis. Citocina Polimorfismo genético Tuberculose. |
| description |
Some single-base polymorphisms (SNPs) in cytokine genes have the potential to affect gene expression and/or biological activity of the respective cytokine, and there is evidence of the association of different SNPs with tuberculosis in several different populations. Studies involving the influence of SNPs on the IL17A gene are still controversial, behaving differently among different populations. Thus, research into the association of SNPs in the IL17A gene with the clinical course of the disease may contribute to a better understanding of TB susceptibility and development. Therefore, the aim of the present study was to evaluate the influence of SNPs on the IL17A gene and their clinical-laboratory association in patients with tuberculosis. Therefore, 35 patients with tuberculosis and 26 controls were studied. The evaluation of the SNPs in the IL17A gene, rs7747909 and rs2275913, was performed using the allelic discrimination technique by real-time PCR. Clinical, laboratory and radiological data were obtained by collecting medical records. Analyzes were performed using Fisher's test, considering significant values p<0.05. There was no association of SNPs in the IL17A gene evaluated, either in relation to susceptibility or in terms of clinical, laboratory and radiological association. Therefore, it is essential to advance in research to analyze the influence of other SNPs on the IL17A gene and the relationship with other variables linked to the immune response to M. tuberculosis. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-08-16 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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https://rsdjournal.org/index.php/rsd/article/view/33518 10.33448/rsd-v11i11.33518 |
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https://rsdjournal.org/index.php/rsd/article/view/33518 |
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10.33448/rsd-v11i11.33518 |
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por |
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por |
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https://rsdjournal.org/index.php/rsd/article/view/33518/28232 |
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https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by/4.0 |
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openAccess |
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application/pdf |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development; Vol. 11 No. 11; e95111133518 Research, Society and Development; Vol. 11 Núm. 11; e95111133518 Research, Society and Development; v. 11 n. 11; e95111133518 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
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Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
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