Antibacterial Activity, Antioxidant and Phenolic Compounds of Honeys Produced by Nannotrigona testaceicornis Lepeletier (Apidae, Meliponini)

Detalhes bibliográficos
Autor(a) principal: Carvalho, Érica Larissa Silva
Data de Publicação: 2021
Outros Autores: Bomfim, Ellen Matos Silva, Silva, Marcos da Costa, Lima, Luciene Cristina Lima e, Marques, Edson de Jesus, Vale, Vera Lúcia Costa
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/18424
Resumo: Bee honey is used in folk medicine to treat infections and oxidative damage. The composition of honey is quite variable, as it depends on the type of nectar of the plant species, the species of bee that produces it, the environmental conditions, collection and processing. This study aimed to evaluate the antibacterial and antioxidant activity, the content of total phenolic compounds and total flavonoids in honey from Nannotrigona testaceicornis L. The antibacterial activity was performed by the disk diffusion method and by the minimum inhibitory concentration. The antioxidant capacity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl free radical scavenging assay. The content of total phenols and flavonoids was quantified by spectrophotometry. It was observed that honey samples from May and June/2013 inhibited bacterial growth for all analyzed bacteria (Pseudomonas aeruginosa, Escherichia coli; Micrococcus luteus and Staphylococcus aureus). The ANOVA test showed that there was a statistically significant difference between months for P. aeruginosa; E. coli; M. luteus and S. aureus. In the assay to determine the minimum inhibitory concentration, samples from May/2013, March/2014 and June/2014 were more active against M. luteus. Antioxidant capacity above 60% was observed in the sample from May/2013. The samples from January/2014 and May/2103 stood out in the quantification of total phenols and total flavonoids, respectively. There was a positive correlation between antioxidant action and total flavonoids. Honey in natura from Nannotrigona testaceicornis L. showed antimicrobial potential and free radical scavenging capacity.
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spelling Antibacterial Activity, Antioxidant and Phenolic Compounds of Honeys Produced by Nannotrigona testaceicornis Lepeletier (Apidae, Meliponini)Atividade Antibacteriana, Antioxidante y Compostos Fenólicos de Méis Produzidos por Nannotrigona testaceicornis Lepeletierta (Apidae, Meliponini)Atividade Antibacteriana, Antioxidante e Compostos Fenólicos de Méis Produzidos por Nannotrigona testaceicornis Lepeletier (Apidae, Meliponini)AntimicrobianoAbeja nativaFenoles totalesDPPH.AntimicrobialNative beeTotal phenolsDPPH.AntimicrobianoAbelha nativaFenóis totaisDPPH.Bee honey is used in folk medicine to treat infections and oxidative damage. The composition of honey is quite variable, as it depends on the type of nectar of the plant species, the species of bee that produces it, the environmental conditions, collection and processing. This study aimed to evaluate the antibacterial and antioxidant activity, the content of total phenolic compounds and total flavonoids in honey from Nannotrigona testaceicornis L. The antibacterial activity was performed by the disk diffusion method and by the minimum inhibitory concentration. The antioxidant capacity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl free radical scavenging assay. The content of total phenols and flavonoids was quantified by spectrophotometry. It was observed that honey samples from May and June/2013 inhibited bacterial growth for all analyzed bacteria (Pseudomonas aeruginosa, Escherichia coli; Micrococcus luteus and Staphylococcus aureus). The ANOVA test showed that there was a statistically significant difference between months for P. aeruginosa; E. coli; M. luteus and S. aureus. In the assay to determine the minimum inhibitory concentration, samples from May/2013, March/2014 and June/2014 were more active against M. luteus. Antioxidant capacity above 60% was observed in the sample from May/2013. The samples from January/2014 and May/2103 stood out in the quantification of total phenols and total flavonoids, respectively. There was a positive correlation between antioxidant action and total flavonoids. Honey in natura from Nannotrigona testaceicornis L. showed antimicrobial potential and free radical scavenging capacity.La miel de abeja se usa en la medicina popular para tratar infecciones y daño oxidativo. La composición de la miel es bastante variable, ya que depende del tipo de néctar de la especie vegetal, la especie de abeja que la produce, las condiciones ambientales, recolección y procesamiento. Este estudio tuvo como objetivo evaluar la actividad antibacteriana y antioxidante, el contenido de compuestos fenólicos totales y flavonoides totales en miel de Nannotrigona testaceicornis L. La actividad antibacteriana se realizó por el método de difusión en disco y por la concentración mínima inhibitoria. La capacidad antioxidante se evaluó utilizando el ensayo de eliminación de radicales libres 2,2-difenil-1-picrilhidrazilo. El contenido de fenoles y flavonoides totales se cuantificó mediante espectrofotometría. Se observó que las muestras de miel de mayo y junio de 2013 inhibieron el crecimiento bacteriano de todas las bacterias analizadas (Pseudomonas aeruginosa, Escherichia coli; Micrococcus luteus y Staphylococcus aureus). La prueba ANOVA mostró que hubo una diferencia estadísticamente significativa entre meses para P. aeruginosa; E. coli; M. luteus y S. aureus. En el ensayo para determinar la concentración mínima inhibitoria, las muestras de mayo / 2013, marzo / 2014 y junio / 2014 fueron más activas contra M. luteus. En la muestra de mayo de 2013 se observó una capacidad antioxidante superior al 60%. Las muestras de enero / 2014 y mayo / 2103 se destacaron en la cuantificación de fenoles totales y flavonoides totales, respectivamente. Hubo una correlación positiva entre la acción antioxidante y los flavonoides totales. La miel in natura de Nannotrigona testaceicornis L. mostró potencial antimicrobiano y capacidad de captación de radicales libres.O mel de abelhas vem sendo utilizado na medicina popular para o tratamento de infecções e contra danos oxidativos. Entretanto, a composição do mel é bastante variável, pois depende da composição do néctar da espécie vegetal, da espécie de abelha produtora, das condições ambientais, da coleta e processamento. O presente estudo objetivou avaliar a atividade antibacteriana, antioxidante, teor dos compostos fenólicos totais e flavonoides totais do mel de Nannotrigona testaceicornis L. A atividade antibacteriana foi realizada pelo método de difusão em discos e concentração inibitória mínima. A capacidade antioxidante foi investigada através do ensaio de captura do radical livre 2,2-difenil-1-picrilhidrazila. O teor total de fenóis totais e flavonoides totais foi quantificado por espectrofotometria. As amostras dos meses de maio e junho/2013 inibiram o crescimento bacteriano para todas as bactérias analisadas. Todas as amostras de mel apresentaram ação antibacteriana frente a Pseudomonas aeruginosa. O teste ANOVA mostrou que houve diferença estatisticamente significante entre os meses para as bactérias Pseudomonas aeruginosa; Escherichia coli; Micrococcus luteus e Staphylococcus aureus. No ensaio de determinação da concentração inibitória mínima, as amostras de maio/2013, março/2014 e junho/2014 foram mais ativas frente a Micrococcus luteus. A capacidade antioxidante acima de 60% foi observada na amostra de maio/2013. As amostras de janeiro/2014 e maio/2103 se destacaram na quantificação de fenóis totais e flavonoides totais, respectivamente. Houve correlação positiva entre a ação antioxidante e flavonoides totais. O mel in natura de Nannotrigona testaceicornis L. apresenta potencial antimicrobiano e capacidade sequestradora de radicais livres.Research, Society and Development2021-08-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1842410.33448/rsd-v10i10.18424Research, Society and Development; Vol. 10 No. 10; e48101018424Research, Society and Development; Vol. 10 Núm. 10; e48101018424Research, Society and Development; v. 10 n. 10; e481010184242525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/18424/16575Copyright (c) 2021 Érica Larissa Silva Carvalho; Ellen Matos Silva Bomfim; Marcos da Costa Silva; Luciene Cristina Lima e Lima; Edson de Jesus Marques; Vera Lúcia Costa Valehttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCarvalho, Érica Larissa Silva Bomfim, Ellen Matos Silva Silva, Marcos da Costa Lima, Luciene Cristina Lima e Marques, Edson de Jesus Vale, Vera Lúcia Costa 2021-10-02T21:49:16Zoai:ojs.pkp.sfu.ca:article/18424Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:38:34.138091Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Antibacterial Activity, Antioxidant and Phenolic Compounds of Honeys Produced by Nannotrigona testaceicornis Lepeletier (Apidae, Meliponini)
Atividade Antibacteriana, Antioxidante y Compostos Fenólicos de Méis Produzidos por Nannotrigona testaceicornis Lepeletierta (Apidae, Meliponini)
Atividade Antibacteriana, Antioxidante e Compostos Fenólicos de Méis Produzidos por Nannotrigona testaceicornis Lepeletier (Apidae, Meliponini)
title Antibacterial Activity, Antioxidant and Phenolic Compounds of Honeys Produced by Nannotrigona testaceicornis Lepeletier (Apidae, Meliponini)
spellingShingle Antibacterial Activity, Antioxidant and Phenolic Compounds of Honeys Produced by Nannotrigona testaceicornis Lepeletier (Apidae, Meliponini)
Carvalho, Érica Larissa Silva
Antimicrobiano
Abeja nativa
Fenoles totales
DPPH.
Antimicrobial
Native bee
Total phenols
DPPH.
Antimicrobiano
Abelha nativa
Fenóis totais
DPPH.
title_short Antibacterial Activity, Antioxidant and Phenolic Compounds of Honeys Produced by Nannotrigona testaceicornis Lepeletier (Apidae, Meliponini)
title_full Antibacterial Activity, Antioxidant and Phenolic Compounds of Honeys Produced by Nannotrigona testaceicornis Lepeletier (Apidae, Meliponini)
title_fullStr Antibacterial Activity, Antioxidant and Phenolic Compounds of Honeys Produced by Nannotrigona testaceicornis Lepeletier (Apidae, Meliponini)
title_full_unstemmed Antibacterial Activity, Antioxidant and Phenolic Compounds of Honeys Produced by Nannotrigona testaceicornis Lepeletier (Apidae, Meliponini)
title_sort Antibacterial Activity, Antioxidant and Phenolic Compounds of Honeys Produced by Nannotrigona testaceicornis Lepeletier (Apidae, Meliponini)
author Carvalho, Érica Larissa Silva
author_facet Carvalho, Érica Larissa Silva
Bomfim, Ellen Matos Silva
Silva, Marcos da Costa
Lima, Luciene Cristina Lima e
Marques, Edson de Jesus
Vale, Vera Lúcia Costa
author_role author
author2 Bomfim, Ellen Matos Silva
Silva, Marcos da Costa
Lima, Luciene Cristina Lima e
Marques, Edson de Jesus
Vale, Vera Lúcia Costa
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, Érica Larissa Silva
Bomfim, Ellen Matos Silva
Silva, Marcos da Costa
Lima, Luciene Cristina Lima e
Marques, Edson de Jesus
Vale, Vera Lúcia Costa
dc.subject.por.fl_str_mv Antimicrobiano
Abeja nativa
Fenoles totales
DPPH.
Antimicrobial
Native bee
Total phenols
DPPH.
Antimicrobiano
Abelha nativa
Fenóis totais
DPPH.
topic Antimicrobiano
Abeja nativa
Fenoles totales
DPPH.
Antimicrobial
Native bee
Total phenols
DPPH.
Antimicrobiano
Abelha nativa
Fenóis totais
DPPH.
description Bee honey is used in folk medicine to treat infections and oxidative damage. The composition of honey is quite variable, as it depends on the type of nectar of the plant species, the species of bee that produces it, the environmental conditions, collection and processing. This study aimed to evaluate the antibacterial and antioxidant activity, the content of total phenolic compounds and total flavonoids in honey from Nannotrigona testaceicornis L. The antibacterial activity was performed by the disk diffusion method and by the minimum inhibitory concentration. The antioxidant capacity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl free radical scavenging assay. The content of total phenols and flavonoids was quantified by spectrophotometry. It was observed that honey samples from May and June/2013 inhibited bacterial growth for all analyzed bacteria (Pseudomonas aeruginosa, Escherichia coli; Micrococcus luteus and Staphylococcus aureus). The ANOVA test showed that there was a statistically significant difference between months for P. aeruginosa; E. coli; M. luteus and S. aureus. In the assay to determine the minimum inhibitory concentration, samples from May/2013, March/2014 and June/2014 were more active against M. luteus. Antioxidant capacity above 60% was observed in the sample from May/2013. The samples from January/2014 and May/2103 stood out in the quantification of total phenols and total flavonoids, respectively. There was a positive correlation between antioxidant action and total flavonoids. Honey in natura from Nannotrigona testaceicornis L. showed antimicrobial potential and free radical scavenging capacity.
publishDate 2021
dc.date.none.fl_str_mv 2021-08-05
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/18424
10.33448/rsd-v10i10.18424
url https://rsdjournal.org/index.php/rsd/article/view/18424
identifier_str_mv 10.33448/rsd-v10i10.18424
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/18424/16575
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 10; e48101018424
Research, Society and Development; Vol. 10 Núm. 10; e48101018424
Research, Society and Development; v. 10 n. 10; e48101018424
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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