Antibacterial Activity, Antioxidant and Phenolic Compounds of Honeys Produced by Nannotrigona testaceicornis Lepeletier (Apidae, Meliponini)
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/18424 |
Resumo: | Bee honey is used in folk medicine to treat infections and oxidative damage. The composition of honey is quite variable, as it depends on the type of nectar of the plant species, the species of bee that produces it, the environmental conditions, collection and processing. This study aimed to evaluate the antibacterial and antioxidant activity, the content of total phenolic compounds and total flavonoids in honey from Nannotrigona testaceicornis L. The antibacterial activity was performed by the disk diffusion method and by the minimum inhibitory concentration. The antioxidant capacity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl free radical scavenging assay. The content of total phenols and flavonoids was quantified by spectrophotometry. It was observed that honey samples from May and June/2013 inhibited bacterial growth for all analyzed bacteria (Pseudomonas aeruginosa, Escherichia coli; Micrococcus luteus and Staphylococcus aureus). The ANOVA test showed that there was a statistically significant difference between months for P. aeruginosa; E. coli; M. luteus and S. aureus. In the assay to determine the minimum inhibitory concentration, samples from May/2013, March/2014 and June/2014 were more active against M. luteus. Antioxidant capacity above 60% was observed in the sample from May/2013. The samples from January/2014 and May/2103 stood out in the quantification of total phenols and total flavonoids, respectively. There was a positive correlation between antioxidant action and total flavonoids. Honey in natura from Nannotrigona testaceicornis L. showed antimicrobial potential and free radical scavenging capacity. |
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Antibacterial Activity, Antioxidant and Phenolic Compounds of Honeys Produced by Nannotrigona testaceicornis Lepeletier (Apidae, Meliponini)Atividade Antibacteriana, Antioxidante y Compostos Fenólicos de Méis Produzidos por Nannotrigona testaceicornis Lepeletierta (Apidae, Meliponini)Atividade Antibacteriana, Antioxidante e Compostos Fenólicos de Méis Produzidos por Nannotrigona testaceicornis Lepeletier (Apidae, Meliponini)AntimicrobianoAbeja nativaFenoles totalesDPPH.AntimicrobialNative beeTotal phenolsDPPH.AntimicrobianoAbelha nativaFenóis totaisDPPH.Bee honey is used in folk medicine to treat infections and oxidative damage. The composition of honey is quite variable, as it depends on the type of nectar of the plant species, the species of bee that produces it, the environmental conditions, collection and processing. This study aimed to evaluate the antibacterial and antioxidant activity, the content of total phenolic compounds and total flavonoids in honey from Nannotrigona testaceicornis L. The antibacterial activity was performed by the disk diffusion method and by the minimum inhibitory concentration. The antioxidant capacity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl free radical scavenging assay. The content of total phenols and flavonoids was quantified by spectrophotometry. It was observed that honey samples from May and June/2013 inhibited bacterial growth for all analyzed bacteria (Pseudomonas aeruginosa, Escherichia coli; Micrococcus luteus and Staphylococcus aureus). The ANOVA test showed that there was a statistically significant difference between months for P. aeruginosa; E. coli; M. luteus and S. aureus. In the assay to determine the minimum inhibitory concentration, samples from May/2013, March/2014 and June/2014 were more active against M. luteus. Antioxidant capacity above 60% was observed in the sample from May/2013. The samples from January/2014 and May/2103 stood out in the quantification of total phenols and total flavonoids, respectively. There was a positive correlation between antioxidant action and total flavonoids. Honey in natura from Nannotrigona testaceicornis L. showed antimicrobial potential and free radical scavenging capacity.La miel de abeja se usa en la medicina popular para tratar infecciones y daño oxidativo. La composición de la miel es bastante variable, ya que depende del tipo de néctar de la especie vegetal, la especie de abeja que la produce, las condiciones ambientales, recolección y procesamiento. Este estudio tuvo como objetivo evaluar la actividad antibacteriana y antioxidante, el contenido de compuestos fenólicos totales y flavonoides totales en miel de Nannotrigona testaceicornis L. La actividad antibacteriana se realizó por el método de difusión en disco y por la concentración mínima inhibitoria. La capacidad antioxidante se evaluó utilizando el ensayo de eliminación de radicales libres 2,2-difenil-1-picrilhidrazilo. El contenido de fenoles y flavonoides totales se cuantificó mediante espectrofotometría. Se observó que las muestras de miel de mayo y junio de 2013 inhibieron el crecimiento bacteriano de todas las bacterias analizadas (Pseudomonas aeruginosa, Escherichia coli; Micrococcus luteus y Staphylococcus aureus). La prueba ANOVA mostró que hubo una diferencia estadísticamente significativa entre meses para P. aeruginosa; E. coli; M. luteus y S. aureus. En el ensayo para determinar la concentración mínima inhibitoria, las muestras de mayo / 2013, marzo / 2014 y junio / 2014 fueron más activas contra M. luteus. En la muestra de mayo de 2013 se observó una capacidad antioxidante superior al 60%. Las muestras de enero / 2014 y mayo / 2103 se destacaron en la cuantificación de fenoles totales y flavonoides totales, respectivamente. Hubo una correlación positiva entre la acción antioxidante y los flavonoides totales. La miel in natura de Nannotrigona testaceicornis L. mostró potencial antimicrobiano y capacidad de captación de radicales libres.O mel de abelhas vem sendo utilizado na medicina popular para o tratamento de infecções e contra danos oxidativos. Entretanto, a composição do mel é bastante variável, pois depende da composição do néctar da espécie vegetal, da espécie de abelha produtora, das condições ambientais, da coleta e processamento. O presente estudo objetivou avaliar a atividade antibacteriana, antioxidante, teor dos compostos fenólicos totais e flavonoides totais do mel de Nannotrigona testaceicornis L. A atividade antibacteriana foi realizada pelo método de difusão em discos e concentração inibitória mínima. A capacidade antioxidante foi investigada através do ensaio de captura do radical livre 2,2-difenil-1-picrilhidrazila. O teor total de fenóis totais e flavonoides totais foi quantificado por espectrofotometria. As amostras dos meses de maio e junho/2013 inibiram o crescimento bacteriano para todas as bactérias analisadas. Todas as amostras de mel apresentaram ação antibacteriana frente a Pseudomonas aeruginosa. O teste ANOVA mostrou que houve diferença estatisticamente significante entre os meses para as bactérias Pseudomonas aeruginosa; Escherichia coli; Micrococcus luteus e Staphylococcus aureus. No ensaio de determinação da concentração inibitória mínima, as amostras de maio/2013, março/2014 e junho/2014 foram mais ativas frente a Micrococcus luteus. A capacidade antioxidante acima de 60% foi observada na amostra de maio/2013. As amostras de janeiro/2014 e maio/2103 se destacaram na quantificação de fenóis totais e flavonoides totais, respectivamente. Houve correlação positiva entre a ação antioxidante e flavonoides totais. O mel in natura de Nannotrigona testaceicornis L. apresenta potencial antimicrobiano e capacidade sequestradora de radicais livres.Research, Society and Development2021-08-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1842410.33448/rsd-v10i10.18424Research, Society and Development; Vol. 10 No. 10; e48101018424Research, Society and Development; Vol. 10 Núm. 10; e48101018424Research, Society and Development; v. 10 n. 10; e481010184242525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/18424/16575Copyright (c) 2021 Érica Larissa Silva Carvalho; Ellen Matos Silva Bomfim; Marcos da Costa Silva; Luciene Cristina Lima e Lima; Edson de Jesus Marques; Vera Lúcia Costa Valehttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCarvalho, Érica Larissa Silva Bomfim, Ellen Matos Silva Silva, Marcos da Costa Lima, Luciene Cristina Lima e Marques, Edson de Jesus Vale, Vera Lúcia Costa 2021-10-02T21:49:16Zoai:ojs.pkp.sfu.ca:article/18424Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:38:34.138091Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Antibacterial Activity, Antioxidant and Phenolic Compounds of Honeys Produced by Nannotrigona testaceicornis Lepeletier (Apidae, Meliponini) Atividade Antibacteriana, Antioxidante y Compostos Fenólicos de Méis Produzidos por Nannotrigona testaceicornis Lepeletierta (Apidae, Meliponini) Atividade Antibacteriana, Antioxidante e Compostos Fenólicos de Méis Produzidos por Nannotrigona testaceicornis Lepeletier (Apidae, Meliponini) |
title |
Antibacterial Activity, Antioxidant and Phenolic Compounds of Honeys Produced by Nannotrigona testaceicornis Lepeletier (Apidae, Meliponini) |
spellingShingle |
Antibacterial Activity, Antioxidant and Phenolic Compounds of Honeys Produced by Nannotrigona testaceicornis Lepeletier (Apidae, Meliponini) Carvalho, Érica Larissa Silva Antimicrobiano Abeja nativa Fenoles totales DPPH. Antimicrobial Native bee Total phenols DPPH. Antimicrobiano Abelha nativa Fenóis totais DPPH. |
title_short |
Antibacterial Activity, Antioxidant and Phenolic Compounds of Honeys Produced by Nannotrigona testaceicornis Lepeletier (Apidae, Meliponini) |
title_full |
Antibacterial Activity, Antioxidant and Phenolic Compounds of Honeys Produced by Nannotrigona testaceicornis Lepeletier (Apidae, Meliponini) |
title_fullStr |
Antibacterial Activity, Antioxidant and Phenolic Compounds of Honeys Produced by Nannotrigona testaceicornis Lepeletier (Apidae, Meliponini) |
title_full_unstemmed |
Antibacterial Activity, Antioxidant and Phenolic Compounds of Honeys Produced by Nannotrigona testaceicornis Lepeletier (Apidae, Meliponini) |
title_sort |
Antibacterial Activity, Antioxidant and Phenolic Compounds of Honeys Produced by Nannotrigona testaceicornis Lepeletier (Apidae, Meliponini) |
author |
Carvalho, Érica Larissa Silva |
author_facet |
Carvalho, Érica Larissa Silva Bomfim, Ellen Matos Silva Silva, Marcos da Costa Lima, Luciene Cristina Lima e Marques, Edson de Jesus Vale, Vera Lúcia Costa |
author_role |
author |
author2 |
Bomfim, Ellen Matos Silva Silva, Marcos da Costa Lima, Luciene Cristina Lima e Marques, Edson de Jesus Vale, Vera Lúcia Costa |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Carvalho, Érica Larissa Silva Bomfim, Ellen Matos Silva Silva, Marcos da Costa Lima, Luciene Cristina Lima e Marques, Edson de Jesus Vale, Vera Lúcia Costa |
dc.subject.por.fl_str_mv |
Antimicrobiano Abeja nativa Fenoles totales DPPH. Antimicrobial Native bee Total phenols DPPH. Antimicrobiano Abelha nativa Fenóis totais DPPH. |
topic |
Antimicrobiano Abeja nativa Fenoles totales DPPH. Antimicrobial Native bee Total phenols DPPH. Antimicrobiano Abelha nativa Fenóis totais DPPH. |
description |
Bee honey is used in folk medicine to treat infections and oxidative damage. The composition of honey is quite variable, as it depends on the type of nectar of the plant species, the species of bee that produces it, the environmental conditions, collection and processing. This study aimed to evaluate the antibacterial and antioxidant activity, the content of total phenolic compounds and total flavonoids in honey from Nannotrigona testaceicornis L. The antibacterial activity was performed by the disk diffusion method and by the minimum inhibitory concentration. The antioxidant capacity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl free radical scavenging assay. The content of total phenols and flavonoids was quantified by spectrophotometry. It was observed that honey samples from May and June/2013 inhibited bacterial growth for all analyzed bacteria (Pseudomonas aeruginosa, Escherichia coli; Micrococcus luteus and Staphylococcus aureus). The ANOVA test showed that there was a statistically significant difference between months for P. aeruginosa; E. coli; M. luteus and S. aureus. In the assay to determine the minimum inhibitory concentration, samples from May/2013, March/2014 and June/2014 were more active against M. luteus. Antioxidant capacity above 60% was observed in the sample from May/2013. The samples from January/2014 and May/2103 stood out in the quantification of total phenols and total flavonoids, respectively. There was a positive correlation between antioxidant action and total flavonoids. Honey in natura from Nannotrigona testaceicornis L. showed antimicrobial potential and free radical scavenging capacity. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08-05 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/18424 10.33448/rsd-v10i10.18424 |
url |
https://rsdjournal.org/index.php/rsd/article/view/18424 |
identifier_str_mv |
10.33448/rsd-v10i10.18424 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/18424/16575 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 10; e48101018424 Research, Society and Development; Vol. 10 Núm. 10; e48101018424 Research, Society and Development; v. 10 n. 10; e48101018424 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052684740591616 |