Análise química de extratos de Caryocar brasiliense com potencial antioxidante

• Autor(a) principal: Ferreira, Claudiane Marques
• Data de Publicação: 2019
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• dARK ID: ark:/38995/001300000bv7j
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/9699
• topic: Capacidade antioxidante; Cromatografia; DPPH; Fenois totais; Pequi; Antioxidant capacity; Chromatography; DPPH; Total phenols; Pequi; CIENCIAS AGRARIAS::MEDICINA VETERINARIA

The pequi (Caryocar brasiliense) is a typical Brazilian fruit that stands out for its high nutritional value, besides its sensorial attributes like flavor, aroma and color. The fruit peel is a byproduct of industrial processing and has potential for explotation due to the presence of phenolic compounds that act in the animal body as antioxidants, capable of preventing or preventing the action of free radicals that cause degenerative diseases. The objective of this work was to analyze the presence of phenolic compounds and their antioxidant activities through chemical analyzes of crude ethanolic extract and its hexane, dichloromethane, ethyl acetate and aqueous fractions of Caryocar brasiliense bark. The identification of phenolic compounds was performed by high performance liquid chromatography coupled to high resolution mass spectrometry. Phenol content was analyzed by determination of the total phenolic profile by the Folin-Ciocalteu method and the antioxidant capacity was analyzed by the in vitro antioxidant activity method by the DPPH assay. A total of fifteen phenolic compounds were identified by comparison with the standards: gallic, ellagic, protocatechic, gentilic, vanillic, caffeic, ferulic and p-coumaric acids. In addition to catechin, epicatechin, luteolin, kaempferol, quercetin, rutin and naringerin. The dichloromethane, ethyl acetate and aqueous fractions showed thirteen of the fifteen phenolic compounds, the crude extract twelve and the hexane ten. The total phenol profile ranged from 0.6 to 48 mg / 100 mL and the in vitro antioxidant activity showed high values ranging from 26% to 99.4%. The results confirm that the crude extract of the bark of the Caryocar brasiliense fruit and its hexane, dichloromethane, ethyl acetate and aqueous fractions have phenolic compounds and antioxidant activity.