Chemical analysis and insecticidal activity of Ocimum gratissimum essential oil and its major constituent against Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae)
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/9787 |
Resumo: | The fall armyworm, Spodoptera frugiperda, causes damage at several stages of the maize crop cycle. Due to its resistance to synthetic insecticides and the high costs of pest control, there is an ever-increasing amount of research on alternative or complementary products that have a minor environmental and financial impact on agriculture. Therefore, the aim of this study was to evaluate the chemical composition and insecticidal potential of Ocimum gratissimum (african basil) leaves essential oil and the effect of its major component, thymol, on S. frugiperda control. Gas Chromatography (GC) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis identified p-cymene, γ-terpinene, and thymol compounds as the main constituents of the oil, which presented a yield of 4.75%. Among the 30 identified compounds, thymol (33.2%) was the major constituent, representing 97.8% of the total oil. The efficacy of both the oil and thymol standard (Sigma-Aldrich) was evaluated against S. frugiperda using topical acute toxicity and contact surface tests at different concentrations. The oil was more active than thymol standard, with topical acute toxicity of LD50 at 0.020 μl/insect and LC50 at 0.171 μl/cm2 for contact surface toxicity. The oil proved to be superior to the thymol standard, offering an effective and promising alternative for the control of S. frugiperda, which is most likely due to the contribution of other oil components that acted synergistically. Consequently, this result provides an opportunity for further study and the development of an effective fall armyworm control system. |
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Chemical analysis and insecticidal activity of Ocimum gratissimum essential oil and its major constituent against Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae)Análisis químico y actividad insecticida del aceite esencial de Ocimum gratissimum y su principal constituyente contra Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae)Análise química e atividade inseticida do óleo essencial de Ocimum gratissimum e de seu constituinte majoritário contra Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae)Fall armywormBotanical insecticideChemical constituentsLamiaceae.Cartucho de orugaInsecticida botánicoComponentes químicosLamiaceae.Lagarta do cartuchoInseticida botânicoConstituintes químicosLamiaceae.The fall armyworm, Spodoptera frugiperda, causes damage at several stages of the maize crop cycle. Due to its resistance to synthetic insecticides and the high costs of pest control, there is an ever-increasing amount of research on alternative or complementary products that have a minor environmental and financial impact on agriculture. Therefore, the aim of this study was to evaluate the chemical composition and insecticidal potential of Ocimum gratissimum (african basil) leaves essential oil and the effect of its major component, thymol, on S. frugiperda control. Gas Chromatography (GC) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis identified p-cymene, γ-terpinene, and thymol compounds as the main constituents of the oil, which presented a yield of 4.75%. Among the 30 identified compounds, thymol (33.2%) was the major constituent, representing 97.8% of the total oil. The efficacy of both the oil and thymol standard (Sigma-Aldrich) was evaluated against S. frugiperda using topical acute toxicity and contact surface tests at different concentrations. The oil was more active than thymol standard, with topical acute toxicity of LD50 at 0.020 μl/insect and LC50 at 0.171 μl/cm2 for contact surface toxicity. The oil proved to be superior to the thymol standard, offering an effective and promising alternative for the control of S. frugiperda, which is most likely due to the contribution of other oil components that acted synergistically. Consequently, this result provides an opportunity for further study and the development of an effective fall armyworm control system.El cartucho de oruga, Spodoptera frugiperda, causa daños en varias etapas del ciclo del cultivo del maíz. Debido a su resistencia a los insecticidas sintéticos y los altos costos del control de plagas, existe un número creciente de investigaciones sobre productos alternativos con menor impacto ambiental y financiero en la agricultura. Por tanto, el objetivo de este estudio fue evaluar la composición química y el potencial insecticida del aceite esencial de hojas de Ocimum gratissimum (albahaca africana) y el efecto de su componente principal, el timol, en el control de S. frugiperda. Los análisis de cromatografía de gases (CG) y cromatografía de gases-espectrometría de masas (CG-EM) identificaron los compuestos p-cimeno, γ-terpineno y timol como los principales constituyentes del aceite, con un rendimiento del 4,75%. Entre los 30 compuestos identificados, el timol (33,2%) fue el constituyente principal, representando el 97,8% del aceite total. La efectividad del aceite y el timol estándar (Sigma-Aldrich) se evaluó contra S. frugiperda usando pruebas de toxicidad tópica aguda y superficies de contacto a diferentes concentraciones. El aceite fue más activo que el timol estándar, con toxicidad tópica aguda de LD50 a 0.020 μL/insecto y LC50 a 0.171 μL/cm2 para la toxicidad de la superficie de contacto. El aceite demostró ser superior al estándar de timol, ofreciendo una alternativa efectiva y prometedora para el control de S. frugiperda, lo que probablemente se deba al aporte de otros componentes del aceite que actuaron sinérgicamente. En consecuencia, este resultado brinda la oportunidad de realizar más estudios y desarrollar un sistema de control de oruga de cartucho eficaz.A lagarta-do-cartucho, Spodoptera frugiperda, causa danos em vários estágios do ciclo da cultura do milho. Devido à sua resistência aos inseticidas sintéticos e aos altos custos do controle de pragas, há um número cada vez maior de pesquisas sobre produtos alternativos com menor impacto ambiental e financeiro na agricultura. Portanto, o objetivo deste estudo foi avaliar a composição química e o potencial inseticida do óleo essencial de folhas de Ocimum gratissimum (manjericão africano) e o efeito de seu principal componente, o timol, no controle de S. frugiperda. As análises por Cromatografia Gasosa (CG) e Cromatografia Gasosa-Espectrometria de Massa (CG-EM) identificaram os compostos p-cimeno, γ-terpineno e timol como principais constituintes do óleo, que apresentou rendimento de 4,75%. Entre os 30 compostos identificados, o timol (33,2%) foi o principal constituinte, representando 97,8% do óleo total. A eficácia do óleo e do timol padrão (Sigma-Aldrich) foi avaliada contra S. frugiperda usando toxicidade aguda tópica e testes de superfície de contato em diferentes concentrações. O óleo foi mais ativo do que o timol padrão, com toxicidade aguda tópica de LD50 em 0,020 μL/inseto e LC50 em 0,171 μL/cm2 para toxicidade de superfície de contato. O óleo mostrou-se superior ao padrão timol, oferecendo uma alternativa eficaz e promissora para o controle de S. frugiperda, o que provavelmente se deve à contribuição de outros componentes do óleo que atuaram sinergicamente. Consequentemente, este resultado fornece uma oportunidade para estudos adicionais e o desenvolvimento de um sistema de controle eficaz da lagarta do cartucho.Research, Society and Development2020-11-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/978710.33448/rsd-v9i11.9787Research, Society and Development; Vol. 9 No. 11; e4999119787Research, Society and Development; Vol. 9 Núm. 11; e4999119787Research, Society and Development; v. 9 n. 11; e49991197872525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/9787/9033Copyright (c) 2020 Ildenice Nogueira Monteiro; Odair dos Santos Monteiro; Ademir Kleber Morbeck de Oliveira; Silvio Favero; Nayara Zielasko Trombini Garcia; Yan Michel Lopes Fernandes; Gislayne Santana Santos Jacinto; Carla Letícia Gediel Rivero-Wendt; Rosemary Matiashttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccess Monteiro, Ildenice NogueiraMonteiro, Odair dos Santos Oliveira, Ademir Kleber Morbeck de Favero, Silvio Garcia, Nayara Zielasko Trombini Fernandes, Yan Michel Lopes Jacinto, Gislayne Santana Santos Rivero-Wendt, Carla Letícia GedielMatias, Rosemary 2020-12-10T23:37:57Zoai:ojs.pkp.sfu.ca:article/9787Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:31:59.221669Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Chemical analysis and insecticidal activity of Ocimum gratissimum essential oil and its major constituent against Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae) Análisis químico y actividad insecticida del aceite esencial de Ocimum gratissimum y su principal constituyente contra Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae) Análise química e atividade inseticida do óleo essencial de Ocimum gratissimum e de seu constituinte majoritário contra Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae) |
title |
Chemical analysis and insecticidal activity of Ocimum gratissimum essential oil and its major constituent against Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae) |
spellingShingle |
Chemical analysis and insecticidal activity of Ocimum gratissimum essential oil and its major constituent against Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae) Monteiro, Ildenice Nogueira Fall armyworm Botanical insecticide Chemical constituents Lamiaceae. Cartucho de oruga Insecticida botánico Componentes químicos Lamiaceae. Lagarta do cartucho Inseticida botânico Constituintes químicos Lamiaceae. |
title_short |
Chemical analysis and insecticidal activity of Ocimum gratissimum essential oil and its major constituent against Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae) |
title_full |
Chemical analysis and insecticidal activity of Ocimum gratissimum essential oil and its major constituent against Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae) |
title_fullStr |
Chemical analysis and insecticidal activity of Ocimum gratissimum essential oil and its major constituent against Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae) |
title_full_unstemmed |
Chemical analysis and insecticidal activity of Ocimum gratissimum essential oil and its major constituent against Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae) |
title_sort |
Chemical analysis and insecticidal activity of Ocimum gratissimum essential oil and its major constituent against Spodoptera frugiperda (Smith, 1797) (Lepidoptera: Noctuidae) |
author |
Monteiro, Ildenice Nogueira |
author_facet |
Monteiro, Ildenice Nogueira Monteiro, Odair dos Santos Oliveira, Ademir Kleber Morbeck de Favero, Silvio Garcia, Nayara Zielasko Trombini Fernandes, Yan Michel Lopes Jacinto, Gislayne Santana Santos Rivero-Wendt, Carla Letícia Gediel Matias, Rosemary |
author_role |
author |
author2 |
Monteiro, Odair dos Santos Oliveira, Ademir Kleber Morbeck de Favero, Silvio Garcia, Nayara Zielasko Trombini Fernandes, Yan Michel Lopes Jacinto, Gislayne Santana Santos Rivero-Wendt, Carla Letícia Gediel Matias, Rosemary |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Monteiro, Ildenice Nogueira Monteiro, Odair dos Santos Oliveira, Ademir Kleber Morbeck de Favero, Silvio Garcia, Nayara Zielasko Trombini Fernandes, Yan Michel Lopes Jacinto, Gislayne Santana Santos Rivero-Wendt, Carla Letícia Gediel Matias, Rosemary |
dc.subject.por.fl_str_mv |
Fall armyworm Botanical insecticide Chemical constituents Lamiaceae. Cartucho de oruga Insecticida botánico Componentes químicos Lamiaceae. Lagarta do cartucho Inseticida botânico Constituintes químicos Lamiaceae. |
topic |
Fall armyworm Botanical insecticide Chemical constituents Lamiaceae. Cartucho de oruga Insecticida botánico Componentes químicos Lamiaceae. Lagarta do cartucho Inseticida botânico Constituintes químicos Lamiaceae. |
description |
The fall armyworm, Spodoptera frugiperda, causes damage at several stages of the maize crop cycle. Due to its resistance to synthetic insecticides and the high costs of pest control, there is an ever-increasing amount of research on alternative or complementary products that have a minor environmental and financial impact on agriculture. Therefore, the aim of this study was to evaluate the chemical composition and insecticidal potential of Ocimum gratissimum (african basil) leaves essential oil and the effect of its major component, thymol, on S. frugiperda control. Gas Chromatography (GC) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis identified p-cymene, γ-terpinene, and thymol compounds as the main constituents of the oil, which presented a yield of 4.75%. Among the 30 identified compounds, thymol (33.2%) was the major constituent, representing 97.8% of the total oil. The efficacy of both the oil and thymol standard (Sigma-Aldrich) was evaluated against S. frugiperda using topical acute toxicity and contact surface tests at different concentrations. The oil was more active than thymol standard, with topical acute toxicity of LD50 at 0.020 μl/insect and LC50 at 0.171 μl/cm2 for contact surface toxicity. The oil proved to be superior to the thymol standard, offering an effective and promising alternative for the control of S. frugiperda, which is most likely due to the contribution of other oil components that acted synergistically. Consequently, this result provides an opportunity for further study and the development of an effective fall armyworm control system. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11-22 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/9787 10.33448/rsd-v9i11.9787 |
url |
https://rsdjournal.org/index.php/rsd/article/view/9787 |
identifier_str_mv |
10.33448/rsd-v9i11.9787 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/9787/9033 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 9 No. 11; e4999119787 Research, Society and Development; Vol. 9 Núm. 11; e4999119787 Research, Society and Development; v. 9 n. 11; e4999119787 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052804386258944 |