Evaluation of the microbiota present in toothbrushes of infant patients during hospitalization: Pilot study
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/17615 |
Resumo: | This study aimed to evaluate the possible nosocomial bacterial contamination of toothbrushes from pediatric patients and correlate these findings with the storage conditions. The study was developed with patients admitted to a public hospital in Rio de Janeiro. Only patients hospitalized for at least three days in the pediatric ward were included. Oncologic, immunosuppressed, and patients with severe respiratory infections were excluded. Ten personal toothbrushes were collected and immediately transferred to the Microbiology Laboratory. Bacterial identification was carried out by selective culture media, Gram stain, and biochemical tests (urea hydrolysis, indole, catalase, and conjugated coagulase). Furthermore, antimicrobial sensitivity tests were performed to detect methicillin-resistant and vancomycin-resistant Staphylococcus aureus. Patient's age and hospitalization period were 10.5 years and 8.8 days in mean. Thirty-three toothbrushes were stored exposed to the hospital environment, without any protection. Acinetobacter spp. were identified in 33% and Pseudomonas aeruginosa in 22% of the samples. Additionally, a total of 11% of samples were contaminated by methicillin-resistant and vancomycin-resistant Staphylococcus aureus. Nosocomial bacterial contamination of toothbrushes, including by antibiotic-resistant Staphylococcus aureus, was frequent and strongly associated with their direct exposure to the hospital environment. These results emphasize the importance of implementing strategies to prevent the bacteria contamination of hospitalized patient’s toothbrushes. |
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Evaluation of the microbiota present in toothbrushes of infant patients during hospitalization: Pilot studyEvaluación de la microbiota presente en cepillos de dientes de pacientes pediátricos durante la hospitalización: Estudio pilotoAvaliação da microbiota presente nas escovas dentais de pacientes infantis durante a internação hospitalar: Estudo pilotoEscovação dentáriaContaminaçãoInfecção hospitalar.Cepillado de dientesContaminaciónInfección hospitalaria.ToothbrushingContaminationHospital infection.This study aimed to evaluate the possible nosocomial bacterial contamination of toothbrushes from pediatric patients and correlate these findings with the storage conditions. The study was developed with patients admitted to a public hospital in Rio de Janeiro. Only patients hospitalized for at least three days in the pediatric ward were included. Oncologic, immunosuppressed, and patients with severe respiratory infections were excluded. Ten personal toothbrushes were collected and immediately transferred to the Microbiology Laboratory. Bacterial identification was carried out by selective culture media, Gram stain, and biochemical tests (urea hydrolysis, indole, catalase, and conjugated coagulase). Furthermore, antimicrobial sensitivity tests were performed to detect methicillin-resistant and vancomycin-resistant Staphylococcus aureus. Patient's age and hospitalization period were 10.5 years and 8.8 days in mean. Thirty-three toothbrushes were stored exposed to the hospital environment, without any protection. Acinetobacter spp. were identified in 33% and Pseudomonas aeruginosa in 22% of the samples. Additionally, a total of 11% of samples were contaminated by methicillin-resistant and vancomycin-resistant Staphylococcus aureus. Nosocomial bacterial contamination of toothbrushes, including by antibiotic-resistant Staphylococcus aureus, was frequent and strongly associated with their direct exposure to the hospital environment. These results emphasize the importance of implementing strategies to prevent the bacteria contamination of hospitalized patient’s toothbrushes.Este estudio tuvo como objetivo evaluar la posible contaminación bacteriana nosocomial de los cepillos de dientes de pacientes pediátricos y correlacionar estos hallazgos con las condiciones de almacenamiento. El estudio se desarrolló con pacientes ingresados en un hospital público de Río de Janeiro. Solo se incluyeron pacientes hospitalizados durante al menos tres días en la sala de pediatría. Se excluyeron pacientes oncológicos, inmunosuprimidos y con infecciones respiratorias graves. Se recogieron diez cepillos de dientes personales y se trasladaron inmediatamente al Laboratorio de Microbiología. La identificación bacteriana se realizó mediante medios de cultivo selectivos, tinción de Gram y pruebas bioquímicas (hidrólisis de urea, indol, catalasa y coagulasa conjugada). Además, se realizaron pruebas de sensibilidad a los antimicrobianos para detectar Staphylococcus aureus resistente a la meticilina y a la vancomicina. La edad del paciente y el período de internación fueron de 10,5 años y 8,8 días de media. El treinta y tres por ciento de los cepillos de dientes se almacenaron expuestos al ambiente hospitalario, sin ninguna protección. Acinetobacter spp. se identificaron en el 33% y Pseudomonas aeruginosa en el 22% de las muestras. Además, un total del 11% de las muestras estaban contaminadas por Staphylococcus aureus resistente a la meticilina y a la vancomicina. La contaminación bacteriana nosocomial de los cepillos de dientes, incluso por Staphylococcus aureus resistente a los antibióticos, fue frecuente y estuvo fuertemente asociada con su exposición directa al ambiente hospitalario. Estos resultados enfatizan la importancia de implementar estrategias para prevenir la contaminación bacteriana de los cepillos de dientes de los pacientes hospitalizados.Este estudo teve como objetivo avaliar a possível contaminação bacteriana nosocomial de escovas dentais de pacientes pediátricos e correlacionar esses achados com as condições de armazenamento. O estudo foi desenvolvido com pacientes internados em um hospital público do Rio de Janeiro. Apenas pacientes internados por pelo menos três dias na enfermaria pediátrica foram incluídos. Pacientes oncológicos, imunossuprimidos e com infecções respiratórias graves foram excluídos. Dez escovas de dente pessoais foram coletadas e imediatamente transferidas para o Laboratório de Microbiologia. A identificação bacteriana foi realizada por meio de cultura seletiva, coloração de Gram e testes bioquímicos (hidrólise de uréia, indol, catalase e coagulase conjugada). Além disso, testes de sensibilidade antimicrobiana foram realizados para detectar Staphylococcus aureus resistente à meticilina e resistente à vancomicina. A idade do paciente e o tempo de internação foram de 10,5 anos e em média 8,8 dias. Trinta e três por cento das escovas dentais foram armazenadas expostas ao ambiente hospitalar, sem qualquer proteção. Acinetobacter spp. foram identificados em 33% e Pseudomonas aeruginosa em 22% das amostras. Além disso, um total de 11% das amostras estavam contaminadas por Staphylococcus aureus resistente à meticilina e resistente à vancomicina. A contaminação bacteriana hospitalar de escovas dentais, inclusive por Staphylococcus aureus resistente a antibióticos, foi frequente e fortemente associada à sua exposição direta ao ambiente hospitalar. Esses resultados enfatizam a importância da implementação de estratégias para prevenir a contaminação bacteriana das escovas de dente de pacientes hospitalizados.Research, Society and Development2021-07-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1761510.33448/rsd-v10i9.17615Research, Society and Development; Vol. 10 No. 9; e4210917615Research, Society and Development; Vol. 10 Núm. 9; e4210917615Research, Society and Development; v. 10 n. 9; e42109176152525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/17615/15877Copyright (c) 2021 Diego de Andrade Teixeira; Larissa Parada Leite; Silvio Jorge Machado; Leila Chevitarese; Luciana Alves Herdy da Silva; Flávia Cariús Tesch Ferreira Alves; José Massao Miasatohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessTeixeira, Diego de Andrade Leite, Larissa Parada Jorge Machado, Silvio Chevitarese, LeilaSilva, Luciana Alves Herdy da Alves, Flávia Cariús Tesch Ferreira Miasato, José Massao 2021-09-12T14:28:06Zoai:ojs.pkp.sfu.ca:article/17615Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:37:55.295817Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Evaluation of the microbiota present in toothbrushes of infant patients during hospitalization: Pilot study Evaluación de la microbiota presente en cepillos de dientes de pacientes pediátricos durante la hospitalización: Estudio piloto Avaliação da microbiota presente nas escovas dentais de pacientes infantis durante a internação hospitalar: Estudo piloto |
title |
Evaluation of the microbiota present in toothbrushes of infant patients during hospitalization: Pilot study |
spellingShingle |
Evaluation of the microbiota present in toothbrushes of infant patients during hospitalization: Pilot study Teixeira, Diego de Andrade Escovação dentária Contaminação Infecção hospitalar. Cepillado de dientes Contaminación Infección hospitalaria. Toothbrushing Contamination Hospital infection. |
title_short |
Evaluation of the microbiota present in toothbrushes of infant patients during hospitalization: Pilot study |
title_full |
Evaluation of the microbiota present in toothbrushes of infant patients during hospitalization: Pilot study |
title_fullStr |
Evaluation of the microbiota present in toothbrushes of infant patients during hospitalization: Pilot study |
title_full_unstemmed |
Evaluation of the microbiota present in toothbrushes of infant patients during hospitalization: Pilot study |
title_sort |
Evaluation of the microbiota present in toothbrushes of infant patients during hospitalization: Pilot study |
author |
Teixeira, Diego de Andrade |
author_facet |
Teixeira, Diego de Andrade Leite, Larissa Parada Jorge Machado, Silvio Chevitarese, Leila Silva, Luciana Alves Herdy da Alves, Flávia Cariús Tesch Ferreira Miasato, José Massao |
author_role |
author |
author2 |
Leite, Larissa Parada Jorge Machado, Silvio Chevitarese, Leila Silva, Luciana Alves Herdy da Alves, Flávia Cariús Tesch Ferreira Miasato, José Massao |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Teixeira, Diego de Andrade Leite, Larissa Parada Jorge Machado, Silvio Chevitarese, Leila Silva, Luciana Alves Herdy da Alves, Flávia Cariús Tesch Ferreira Miasato, José Massao |
dc.subject.por.fl_str_mv |
Escovação dentária Contaminação Infecção hospitalar. Cepillado de dientes Contaminación Infección hospitalaria. Toothbrushing Contamination Hospital infection. |
topic |
Escovação dentária Contaminação Infecção hospitalar. Cepillado de dientes Contaminación Infección hospitalaria. Toothbrushing Contamination Hospital infection. |
description |
This study aimed to evaluate the possible nosocomial bacterial contamination of toothbrushes from pediatric patients and correlate these findings with the storage conditions. The study was developed with patients admitted to a public hospital in Rio de Janeiro. Only patients hospitalized for at least three days in the pediatric ward were included. Oncologic, immunosuppressed, and patients with severe respiratory infections were excluded. Ten personal toothbrushes were collected and immediately transferred to the Microbiology Laboratory. Bacterial identification was carried out by selective culture media, Gram stain, and biochemical tests (urea hydrolysis, indole, catalase, and conjugated coagulase). Furthermore, antimicrobial sensitivity tests were performed to detect methicillin-resistant and vancomycin-resistant Staphylococcus aureus. Patient's age and hospitalization period were 10.5 years and 8.8 days in mean. Thirty-three toothbrushes were stored exposed to the hospital environment, without any protection. Acinetobacter spp. were identified in 33% and Pseudomonas aeruginosa in 22% of the samples. Additionally, a total of 11% of samples were contaminated by methicillin-resistant and vancomycin-resistant Staphylococcus aureus. Nosocomial bacterial contamination of toothbrushes, including by antibiotic-resistant Staphylococcus aureus, was frequent and strongly associated with their direct exposure to the hospital environment. These results emphasize the importance of implementing strategies to prevent the bacteria contamination of hospitalized patient’s toothbrushes. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-07-20 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/17615 10.33448/rsd-v10i9.17615 |
url |
https://rsdjournal.org/index.php/rsd/article/view/17615 |
identifier_str_mv |
10.33448/rsd-v10i9.17615 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/17615/15877 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 9; e4210917615 Research, Society and Development; Vol. 10 Núm. 9; e4210917615 Research, Society and Development; v. 10 n. 9; e4210917615 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052752151445504 |