Evaluation of the microbiota present in toothbrushes of infant patients during hospitalization: Pilot study

Detalhes bibliográficos
Autor(a) principal: Teixeira, Diego de Andrade
Data de Publicação: 2021
Outros Autores: Leite, Larissa Parada, Jorge Machado, Silvio, Chevitarese, Leila, Silva, Luciana Alves Herdy da, Alves, Flávia Cariús Tesch Ferreira, Miasato, José Massao
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/17615
Resumo: This study aimed to evaluate the possible nosocomial bacterial contamination of toothbrushes from pediatric patients and correlate these findings with the storage conditions. The study was developed with patients admitted to a public hospital in Rio de Janeiro. Only patients hospitalized for at least three days in the pediatric ward were included. Oncologic, immunosuppressed, and patients with severe respiratory infections were excluded. Ten personal toothbrushes were collected and immediately transferred to the Microbiology Laboratory. Bacterial identification was carried out by selective culture media, Gram stain, and biochemical tests (urea hydrolysis, indole, catalase, and conjugated coagulase). Furthermore, antimicrobial sensitivity tests were performed to detect methicillin-resistant and vancomycin-resistant Staphylococcus aureus. Patient's age and hospitalization period were 10.5 years and 8.8 days in mean. Thirty-three toothbrushes were stored exposed to the hospital environment, without any protection. Acinetobacter spp. were identified in 33% and Pseudomonas aeruginosa in 22% of the samples. Additionally, a total of 11% of samples were contaminated by methicillin-resistant and vancomycin-resistant Staphylococcus aureus. Nosocomial bacterial contamination of toothbrushes, including by antibiotic-resistant Staphylococcus aureus, was frequent and strongly associated with their direct exposure to the hospital environment. These results emphasize the importance of implementing strategies to prevent the bacteria contamination of hospitalized patient’s toothbrushes.
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spelling Evaluation of the microbiota present in toothbrushes of infant patients during hospitalization: Pilot studyEvaluación de la microbiota presente en cepillos de dientes de pacientes pediátricos durante la hospitalización: Estudio pilotoAvaliação da microbiota presente nas escovas dentais de pacientes infantis durante a internação hospitalar: Estudo pilotoEscovação dentáriaContaminaçãoInfecção hospitalar.Cepillado de dientesContaminaciónInfección hospitalaria.ToothbrushingContaminationHospital infection.This study aimed to evaluate the possible nosocomial bacterial contamination of toothbrushes from pediatric patients and correlate these findings with the storage conditions. The study was developed with patients admitted to a public hospital in Rio de Janeiro. Only patients hospitalized for at least three days in the pediatric ward were included. Oncologic, immunosuppressed, and patients with severe respiratory infections were excluded. Ten personal toothbrushes were collected and immediately transferred to the Microbiology Laboratory. Bacterial identification was carried out by selective culture media, Gram stain, and biochemical tests (urea hydrolysis, indole, catalase, and conjugated coagulase). Furthermore, antimicrobial sensitivity tests were performed to detect methicillin-resistant and vancomycin-resistant Staphylococcus aureus. Patient's age and hospitalization period were 10.5 years and 8.8 days in mean. Thirty-three toothbrushes were stored exposed to the hospital environment, without any protection. Acinetobacter spp. were identified in 33% and Pseudomonas aeruginosa in 22% of the samples. Additionally, a total of 11% of samples were contaminated by methicillin-resistant and vancomycin-resistant Staphylococcus aureus. Nosocomial bacterial contamination of toothbrushes, including by antibiotic-resistant Staphylococcus aureus, was frequent and strongly associated with their direct exposure to the hospital environment. These results emphasize the importance of implementing strategies to prevent the bacteria contamination of hospitalized patient’s toothbrushes.Este estudio tuvo como objetivo evaluar la posible contaminación bacteriana nosocomial de los cepillos de dientes de pacientes pediátricos y correlacionar estos hallazgos con las condiciones de almacenamiento. El estudio se desarrolló con pacientes ingresados en un hospital público de Río de Janeiro. Solo se incluyeron pacientes hospitalizados durante al menos tres días en la sala de pediatría. Se excluyeron pacientes oncológicos, inmunosuprimidos y con infecciones respiratorias graves. Se recogieron diez cepillos de dientes personales y se trasladaron inmediatamente al Laboratorio de Microbiología. La identificación bacteriana se realizó mediante medios de cultivo selectivos, tinción de Gram y pruebas bioquímicas (hidrólisis de urea, indol, catalasa y coagulasa conjugada). Además, se realizaron pruebas de sensibilidad a los antimicrobianos para detectar Staphylococcus aureus resistente a la meticilina y a la vancomicina. La edad del paciente y el período de internación fueron de 10,5 años y 8,8 días de media. El treinta y tres por ciento de los cepillos de dientes se almacenaron expuestos al ambiente hospitalario, sin ninguna protección. Acinetobacter spp. se identificaron en el 33% y Pseudomonas aeruginosa en el 22% de las muestras. Además, un total del 11% de las muestras estaban contaminadas por Staphylococcus aureus resistente a la meticilina y a la vancomicina. La contaminación bacteriana nosocomial de los cepillos de dientes, incluso por Staphylococcus aureus resistente a los antibióticos, fue frecuente y estuvo fuertemente asociada con su exposición directa al ambiente hospitalario. Estos resultados enfatizan la importancia de implementar estrategias para prevenir la contaminación bacteriana de los cepillos de dientes de los pacientes hospitalizados.Este estudo teve como objetivo avaliar a possível contaminação bacteriana nosocomial de escovas dentais de pacientes pediátricos e correlacionar esses achados com as condições de armazenamento. O estudo foi desenvolvido com pacientes internados em um hospital público do Rio de Janeiro. Apenas pacientes internados por pelo menos três dias na enfermaria pediátrica foram incluídos. Pacientes oncológicos, imunossuprimidos e com infecções respiratórias graves foram excluídos. Dez escovas de dente pessoais foram coletadas e imediatamente transferidas para o Laboratório de Microbiologia. A identificação bacteriana foi realizada por meio de cultura seletiva, coloração de Gram e testes bioquímicos (hidrólise de uréia, indol, catalase e coagulase conjugada). Além disso, testes de sensibilidade antimicrobiana foram realizados para detectar Staphylococcus aureus resistente à meticilina e resistente à vancomicina. A idade do paciente e o tempo de internação foram de 10,5 anos e em média 8,8 dias. Trinta e três por cento das escovas dentais foram armazenadas expostas ao ambiente hospitalar, sem qualquer proteção. Acinetobacter spp. foram identificados em 33% e Pseudomonas aeruginosa em 22% das amostras. Além disso, um total de 11% das amostras estavam contaminadas por Staphylococcus aureus resistente à meticilina e resistente à vancomicina. A contaminação bacteriana hospitalar de escovas dentais, inclusive por Staphylococcus aureus resistente a antibióticos, foi frequente e fortemente associada à sua exposição direta ao ambiente hospitalar. Esses resultados enfatizam a importância da implementação de estratégias para prevenir a contaminação bacteriana das escovas de dente de pacientes hospitalizados.Research, Society and Development2021-07-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1761510.33448/rsd-v10i9.17615Research, Society and Development; Vol. 10 No. 9; e4210917615Research, Society and Development; Vol. 10 Núm. 9; e4210917615Research, Society and Development; v. 10 n. 9; e42109176152525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/17615/15877Copyright (c) 2021 Diego de Andrade Teixeira; Larissa Parada Leite; Silvio Jorge Machado; Leila Chevitarese; Luciana Alves Herdy da Silva; Flávia Cariús Tesch Ferreira Alves; José Massao Miasatohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessTeixeira, Diego de Andrade Leite, Larissa Parada Jorge Machado, Silvio Chevitarese, LeilaSilva, Luciana Alves Herdy da Alves, Flávia Cariús Tesch Ferreira Miasato, José Massao 2021-09-12T14:28:06Zoai:ojs.pkp.sfu.ca:article/17615Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:37:55.295817Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Evaluation of the microbiota present in toothbrushes of infant patients during hospitalization: Pilot study
Evaluación de la microbiota presente en cepillos de dientes de pacientes pediátricos durante la hospitalización: Estudio piloto
Avaliação da microbiota presente nas escovas dentais de pacientes infantis durante a internação hospitalar: Estudo piloto
title Evaluation of the microbiota present in toothbrushes of infant patients during hospitalization: Pilot study
spellingShingle Evaluation of the microbiota present in toothbrushes of infant patients during hospitalization: Pilot study
Teixeira, Diego de Andrade
Escovação dentária
Contaminação
Infecção hospitalar.
Cepillado de dientes
Contaminación
Infección hospitalaria.
Toothbrushing
Contamination
Hospital infection.
title_short Evaluation of the microbiota present in toothbrushes of infant patients during hospitalization: Pilot study
title_full Evaluation of the microbiota present in toothbrushes of infant patients during hospitalization: Pilot study
title_fullStr Evaluation of the microbiota present in toothbrushes of infant patients during hospitalization: Pilot study
title_full_unstemmed Evaluation of the microbiota present in toothbrushes of infant patients during hospitalization: Pilot study
title_sort Evaluation of the microbiota present in toothbrushes of infant patients during hospitalization: Pilot study
author Teixeira, Diego de Andrade
author_facet Teixeira, Diego de Andrade
Leite, Larissa Parada
Jorge Machado, Silvio
Chevitarese, Leila
Silva, Luciana Alves Herdy da
Alves, Flávia Cariús Tesch Ferreira
Miasato, José Massao
author_role author
author2 Leite, Larissa Parada
Jorge Machado, Silvio
Chevitarese, Leila
Silva, Luciana Alves Herdy da
Alves, Flávia Cariús Tesch Ferreira
Miasato, José Massao
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Teixeira, Diego de Andrade
Leite, Larissa Parada
Jorge Machado, Silvio
Chevitarese, Leila
Silva, Luciana Alves Herdy da
Alves, Flávia Cariús Tesch Ferreira
Miasato, José Massao
dc.subject.por.fl_str_mv Escovação dentária
Contaminação
Infecção hospitalar.
Cepillado de dientes
Contaminación
Infección hospitalaria.
Toothbrushing
Contamination
Hospital infection.
topic Escovação dentária
Contaminação
Infecção hospitalar.
Cepillado de dientes
Contaminación
Infección hospitalaria.
Toothbrushing
Contamination
Hospital infection.
description This study aimed to evaluate the possible nosocomial bacterial contamination of toothbrushes from pediatric patients and correlate these findings with the storage conditions. The study was developed with patients admitted to a public hospital in Rio de Janeiro. Only patients hospitalized for at least three days in the pediatric ward were included. Oncologic, immunosuppressed, and patients with severe respiratory infections were excluded. Ten personal toothbrushes were collected and immediately transferred to the Microbiology Laboratory. Bacterial identification was carried out by selective culture media, Gram stain, and biochemical tests (urea hydrolysis, indole, catalase, and conjugated coagulase). Furthermore, antimicrobial sensitivity tests were performed to detect methicillin-resistant and vancomycin-resistant Staphylococcus aureus. Patient's age and hospitalization period were 10.5 years and 8.8 days in mean. Thirty-three toothbrushes were stored exposed to the hospital environment, without any protection. Acinetobacter spp. were identified in 33% and Pseudomonas aeruginosa in 22% of the samples. Additionally, a total of 11% of samples were contaminated by methicillin-resistant and vancomycin-resistant Staphylococcus aureus. Nosocomial bacterial contamination of toothbrushes, including by antibiotic-resistant Staphylococcus aureus, was frequent and strongly associated with their direct exposure to the hospital environment. These results emphasize the importance of implementing strategies to prevent the bacteria contamination of hospitalized patient’s toothbrushes.
publishDate 2021
dc.date.none.fl_str_mv 2021-07-20
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/17615
10.33448/rsd-v10i9.17615
url https://rsdjournal.org/index.php/rsd/article/view/17615
identifier_str_mv 10.33448/rsd-v10i9.17615
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/17615/15877
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 9; e4210917615
Research, Society and Development; Vol. 10 Núm. 9; e4210917615
Research, Society and Development; v. 10 n. 9; e4210917615
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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