Grafting in peri-implant bone defects by in-situ polymer deposition using a 3D pen – in vitro/ ex vivo study
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Research, Society and Development |
| Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/36234 |
Resumo: | Guided Bone Regeneration (GBR) aims to gain or maintain bone volume due to the use of barrier membranes that act for this purpose. This research aims at grafting polymeric filaments into preformed peri-implant bone defects in porcine condyles in vitro/ex vivo, stabilized and grafted with poly(lactic acid) (PLA) and poly(vinyl alcohol) (PVA) polymeric filaments, printed in-situ with a 3D printing pen. Nine porcine condyles received bone defects of 8 mm diameter and 7 mm depth, where occurred the installation of conical implants of 3.5x10 mm. After forming the bone gap region, above the apical bone anchorage, we divided the Poof Bodies (PB) according to the polymeric fill used: G.Control – without filling in the bone gap; G.PLA – with PLA scaffolds and G.PVA – with PVA scaffolds. In another step, the PVA and PLA 3D membranes were compared with the dense polytetrafluoroethylene membrane (PTFE-d). Subsequently, the SkyScan 1172 microtomograph (Bruker-μCT, Kontich, Belgium) analyzed the PB. The analysis corresponding to the total porosity revealed no statistical difference between G.Control (70.44%), G.PLA (59.99%), and G.PVA (57.66%). The closed porosity showed a statistical difference between G.Control (75.509%) and G.PVA (189.19%) and between G.PVA and G.PLA (79,093%). This study demonstrated the possibility of the polymeric filaments of PVA and PLA to fill the bone defects created, revealing an intimate contact on the surface of the implants used. The data suggested a higher porosity of the PVA filament when applied to bone defects or membrane shape. |
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Grafting in peri-implant bone defects by in-situ polymer deposition using a 3D pen – in vitro/ ex vivo studyInjerto en defectos óseos periimplantares mediante deposición de polímero in-situ a través de un lápiz 3D – estudio in vitro/ ex vivoEnxertia em defeitos ósseos periimplantares por deposição polimérica in-situ através caneta 3D – estudo in vitro/ ex vivoBioimpresiónBiopolímerosImpresión tridimensionalPolímeros.BioimpressãoBiopolímerosImpressão tridimensionalPolímeros.BioprintingBiopolymersPrinting, three-dimensionalPolymers.Guided Bone Regeneration (GBR) aims to gain or maintain bone volume due to the use of barrier membranes that act for this purpose. This research aims at grafting polymeric filaments into preformed peri-implant bone defects in porcine condyles in vitro/ex vivo, stabilized and grafted with poly(lactic acid) (PLA) and poly(vinyl alcohol) (PVA) polymeric filaments, printed in-situ with a 3D printing pen. Nine porcine condyles received bone defects of 8 mm diameter and 7 mm depth, where occurred the installation of conical implants of 3.5x10 mm. After forming the bone gap region, above the apical bone anchorage, we divided the Poof Bodies (PB) according to the polymeric fill used: G.Control – without filling in the bone gap; G.PLA – with PLA scaffolds and G.PVA – with PVA scaffolds. In another step, the PVA and PLA 3D membranes were compared with the dense polytetrafluoroethylene membrane (PTFE-d). Subsequently, the SkyScan 1172 microtomograph (Bruker-μCT, Kontich, Belgium) analyzed the PB. The analysis corresponding to the total porosity revealed no statistical difference between G.Control (70.44%), G.PLA (59.99%), and G.PVA (57.66%). The closed porosity showed a statistical difference between G.Control (75.509%) and G.PVA (189.19%) and between G.PVA and G.PLA (79,093%). This study demonstrated the possibility of the polymeric filaments of PVA and PLA to fill the bone defects created, revealing an intimate contact on the surface of the implants used. The data suggested a higher porosity of the PVA filament when applied to bone defects or membrane shape.El objetivo de la Regeneración Ósea Guiada (ROG) es ganar o mantener el volumen óseo, gracias al uso de membranas de barreras que actúan para tal finalidad. Esta investigación tiene como objetivo el injerto de filamentos de polímeros en defectos óseos periimplantares preformados en cóndilos porcinos in vitro/ex vivo, estabilizados e injertados con filamentos poliméricos poli (ácido láctico) (PLA) y poli (alcohol vinílico) (PVA), impresos in situ con lápiz de impression 3D. Se crearon defectos óseos de 8 mm de diámetro y 7 mm de profundidad en 9 cóndilos porcinos e instalados implantes cónicos de 3.5x10 mm. Después de la formación de la región de gap óseo, encima del anclaje apical óseo, se dividieron los cuerpos de prueba (Cp) de acuerdo al relleno polimérico utilizado: G. Control - sin relleno en el gap óseo; G. PLA – con estructura de PLA y G.PVA – con estructura de PVA. En otra etapa, fueron comparadas las membranas de PVA y PLA 3D con la membrana de politetrafluoretileno denso (PTFE-d). Posteriormente los Cps fueron analizados en el microtomógrafo (Bruker-μCT, Kontich, Belgium). El análisis correspondiente a la porosidad total no reveló diferencia estadísticamente significativa entre G control (70.44%), G.PLA (59.99%), y G.PVA (57.66%). La porosidad cerrada reveló diferencia estadísticamente significativa entre G control (75.509%) y G.PVA (189.19%) y entre G.PVA y G.PLA (79,093%). Este estudio demostró una posibilidad de rellenar los defectos óseos creados mediante filamentos poliméricos de PVA y PLA, revelando un contacto íntimo en la superficie de los implantes usados. Los datos sugieren una alta porosidad de los filamentos de PVA cuando son aplicados en defectos óseos o en la forma de membrana.A Regeneração Óssea Guiada (ROG) objetiva o ganho ou a manutenção do volume ósseo, graças ao uso de membranas de barreira que atuam para tal finalidade. Esta pesquisa visa a enxertia de filamentos poliméricos em defeitos ósseos periimplantares pré-formados em côndilos suínos in vitro/ex vivo, estabilizados e enxertados com filamentos poliméricos poli(ácido lático) (PLA) e poli(álcool vinílico) (PVA), impressos in-situ com caneta de impressão 3D. Foram criados defeitos ósseos de 8 mm de diâmetro e 7 mm de profundidade em 9 côndilos suínos e instalados implantes cônicos de 3.5x10 mm. Após a formação da região de gap ósseo, acima da ancoragem óssea apical, os Corpos de prova (Cp) foram divididos conforme o preenchimento polimérico utilizado: G.Control – sem preenchimento no gap ósseo; G.PLA – arcabouço de PLA e G.PVA – arcabouço de PVA. Em outra etapa, foram comparadas as membranas de PVA e PLA 3D com a membrana de politetrafluoretileno denso (PTFE-d). Posteriormente os Cps foram analisados no microtomógrafo SkyScan 1172 (Bruker-μCT, Kontich, Bélgica). A análise correspondente à porosidade total não revelou diferença estatística entre G.Control (70,44%), G.PLA (59,99%) e G.PVA (57,66%). Já a porosidade fechada revelou diferença estatística entre G.Control (75.509%) e G.PVA (189,199%) e entre G.PVA e G.PLA (79.093%). Este estudo demostrou a possibilidade dos filamentos poliméricos de PVA e PLA preencherem os defeitos ósseos criados, revelando um contato íntimo sobre a superfície dos implantes utilizados. Os dados sugeriram uma maior porosidade do filamento de PVA quando aplicado em defeitos ósseos ou na forma de membrana.Research, Society and Development2022-10-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/3623410.33448/rsd-v11i14.36234Research, Society and Development; Vol. 11 No. 14; e301111436234Research, Society and Development; Vol. 11 Núm. 14; e301111436234Research, Society and Development; v. 11 n. 14; e3011114362342525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/36234/30358Copyright (c) 2022 Alícia Fabro Moraes; Ândrea Leite da Silva Lourençone; Vivyan Cordeiro Goulart; Ellen dos Santos; Walas Cazzassa Vieira; Marcelo Ferreira da Silva; Fabiano Luiz Heggendornhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessMoraes, Alícia Fabro Lourençone, Ândrea Leite da Silva Goulart, Vivyan CordeiroSantos, Ellen dos Vieira, Walas CazzassaSilva, Marcelo Ferreira daHeggendorn, Fabiano Luiz2022-11-08T13:36:27Zoai:ojs.pkp.sfu.ca:article/36234Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:50:51.821917Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
| dc.title.none.fl_str_mv |
Grafting in peri-implant bone defects by in-situ polymer deposition using a 3D pen – in vitro/ ex vivo study Injerto en defectos óseos periimplantares mediante deposición de polímero in-situ a través de un lápiz 3D – estudio in vitro/ ex vivo Enxertia em defeitos ósseos periimplantares por deposição polimérica in-situ através caneta 3D – estudo in vitro/ ex vivo |
| title |
Grafting in peri-implant bone defects by in-situ polymer deposition using a 3D pen – in vitro/ ex vivo study |
| spellingShingle |
Grafting in peri-implant bone defects by in-situ polymer deposition using a 3D pen – in vitro/ ex vivo study Moraes, Alícia Fabro Bioimpresión Biopolímeros Impresión tridimensional Polímeros. Bioimpressão Biopolímeros Impressão tridimensional Polímeros. Bioprinting Biopolymers Printing, three-dimensional Polymers. |
| title_short |
Grafting in peri-implant bone defects by in-situ polymer deposition using a 3D pen – in vitro/ ex vivo study |
| title_full |
Grafting in peri-implant bone defects by in-situ polymer deposition using a 3D pen – in vitro/ ex vivo study |
| title_fullStr |
Grafting in peri-implant bone defects by in-situ polymer deposition using a 3D pen – in vitro/ ex vivo study |
| title_full_unstemmed |
Grafting in peri-implant bone defects by in-situ polymer deposition using a 3D pen – in vitro/ ex vivo study |
| title_sort |
Grafting in peri-implant bone defects by in-situ polymer deposition using a 3D pen – in vitro/ ex vivo study |
| author |
Moraes, Alícia Fabro |
| author_facet |
Moraes, Alícia Fabro Lourençone, Ândrea Leite da Silva Goulart, Vivyan Cordeiro Santos, Ellen dos Vieira, Walas Cazzassa Silva, Marcelo Ferreira da Heggendorn, Fabiano Luiz |
| author_role |
author |
| author2 |
Lourençone, Ândrea Leite da Silva Goulart, Vivyan Cordeiro Santos, Ellen dos Vieira, Walas Cazzassa Silva, Marcelo Ferreira da Heggendorn, Fabiano Luiz |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Moraes, Alícia Fabro Lourençone, Ândrea Leite da Silva Goulart, Vivyan Cordeiro Santos, Ellen dos Vieira, Walas Cazzassa Silva, Marcelo Ferreira da Heggendorn, Fabiano Luiz |
| dc.subject.por.fl_str_mv |
Bioimpresión Biopolímeros Impresión tridimensional Polímeros. Bioimpressão Biopolímeros Impressão tridimensional Polímeros. Bioprinting Biopolymers Printing, three-dimensional Polymers. |
| topic |
Bioimpresión Biopolímeros Impresión tridimensional Polímeros. Bioimpressão Biopolímeros Impressão tridimensional Polímeros. Bioprinting Biopolymers Printing, three-dimensional Polymers. |
| description |
Guided Bone Regeneration (GBR) aims to gain or maintain bone volume due to the use of barrier membranes that act for this purpose. This research aims at grafting polymeric filaments into preformed peri-implant bone defects in porcine condyles in vitro/ex vivo, stabilized and grafted with poly(lactic acid) (PLA) and poly(vinyl alcohol) (PVA) polymeric filaments, printed in-situ with a 3D printing pen. Nine porcine condyles received bone defects of 8 mm diameter and 7 mm depth, where occurred the installation of conical implants of 3.5x10 mm. After forming the bone gap region, above the apical bone anchorage, we divided the Poof Bodies (PB) according to the polymeric fill used: G.Control – without filling in the bone gap; G.PLA – with PLA scaffolds and G.PVA – with PVA scaffolds. In another step, the PVA and PLA 3D membranes were compared with the dense polytetrafluoroethylene membrane (PTFE-d). Subsequently, the SkyScan 1172 microtomograph (Bruker-μCT, Kontich, Belgium) analyzed the PB. The analysis corresponding to the total porosity revealed no statistical difference between G.Control (70.44%), G.PLA (59.99%), and G.PVA (57.66%). The closed porosity showed a statistical difference between G.Control (75.509%) and G.PVA (189.19%) and between G.PVA and G.PLA (79,093%). This study demonstrated the possibility of the polymeric filaments of PVA and PLA to fill the bone defects created, revealing an intimate contact on the surface of the implants used. The data suggested a higher porosity of the PVA filament when applied to bone defects or membrane shape. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-10-27 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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https://rsdjournal.org/index.php/rsd/article/view/36234 10.33448/rsd-v11i14.36234 |
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https://rsdjournal.org/index.php/rsd/article/view/36234 |
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10.33448/rsd-v11i14.36234 |
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eng |
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eng |
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https://rsdjournal.org/index.php/rsd/article/view/36234/30358 |
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https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by/4.0 |
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openAccess |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development; Vol. 11 No. 14; e301111436234 Research, Society and Development; Vol. 11 Núm. 14; e301111436234 Research, Society and Development; v. 11 n. 14; e301111436234 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
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