Evaluation of partial thromboplastin time, thrombin time and prothrombin time over treated plasma using a fibrinolytic protease

Detalhes bibliográficos
Autor(a) principal: Miranda, Vanessa Maria Andrade Machado
Data de Publicação: 2022
Outros Autores: Barbosa Filho, José Pedro Martins, Costa, Romero Marcos Pedrosa Brandão, Leite, Ana Cristina Lima, Oliveira, Vagne de Melo, Batista, Juanize Matias da Silva, Pastrana, Lorenzo, Nascimento, Thiago Pajeú, Porto, Ana Lúcia Figueiredo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/25439
Resumo: Fibrinolytic proteases are enzymes that degrade the fibrin, a main component of the blood clot. Thus, the present study aimed to understand the evaluation of aPTT, TT and PT on plasma treated with a fibrinolytic protease produced by Mucor subtillissimus. To assess the anticoagulant effect, the protease was used in different concentrations of 0.5-2.5mg/mL. The tests showed that the enzyme promoted a significant prolonged time over the PT clotting time as concentration increased. In the aPTT assay, fibrinolytic protease practically did not prolong the clotting time, even with increased enzyme concentration. In TT it was verified that at all times of contact with thrombin, be it 5-30 minutes, there was no interference on the action of thrombin or fibrinogen on the formation of thrombi. Since only the prothrombin time among all those studied was altered, it is suggested that the fibrinolytic protease under study affects the extrinsic pathway of coagulation. Thus, it was possible to observe that the serine protease promoted a significant prolonged time on the clotting time of the PT as the enzyme concentration increased, but the serine protease did not cause any type of change in TT and aPTT.
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spelling Evaluation of partial thromboplastin time, thrombin time and prothrombin time over treated plasma using a fibrinolytic proteaseEvaluación del tiempo de tromboplastina parcial, tiempo de trombina y tiempo de protrombina en plasma tratado con proteasa fibrinolíticaAvaliação do tempo parcial de tromboplastina, tempo de trombina e tempo de protrombina sobre o plasma tratado usando uma protease fibrinolíticaTromboseAgente fibrinolíticoCoagulaçãoPlasma.TrombosisFibrinolíticosCoagulaciónPlasma.ThrombosisFibrinolytic agentsCoagulationPlasma.Fibrinolytic proteases are enzymes that degrade the fibrin, a main component of the blood clot. Thus, the present study aimed to understand the evaluation of aPTT, TT and PT on plasma treated with a fibrinolytic protease produced by Mucor subtillissimus. To assess the anticoagulant effect, the protease was used in different concentrations of 0.5-2.5mg/mL. The tests showed that the enzyme promoted a significant prolonged time over the PT clotting time as concentration increased. In the aPTT assay, fibrinolytic protease practically did not prolong the clotting time, even with increased enzyme concentration. In TT it was verified that at all times of contact with thrombin, be it 5-30 minutes, there was no interference on the action of thrombin or fibrinogen on the formation of thrombi. Since only the prothrombin time among all those studied was altered, it is suggested that the fibrinolytic protease under study affects the extrinsic pathway of coagulation. Thus, it was possible to observe that the serine protease promoted a significant prolonged time on the clotting time of the PT as the enzyme concentration increased, but the serine protease did not cause any type of change in TT and aPTT.Las proteasas fibrinolíticas son enzimas que degradan la fibrina, el principal componente de los coágulos sanguíneos. Así, el presente estudio tuvo como objetivo comprender la evaluación de aPTT, TT y TP en plasma tratado con una proteasa fibrinolítica producida por Mucor subtillissimus. Para evaluar el efecto anticoagulante, se utilizó la proteasa a diferentes concentraciones de 0,5-2,5 mg/ml. Las pruebas mostraron que la enzima promovió un tiempo prolongado significativo durante el tiempo de coagulación de PT con una concentración creciente. En el ensayo APTT, la proteasa fibrinolítica prácticamente no prolongó el tiempo de coagulación, incluso con el aumento de la concentración de enzima. En TT, se encontró que en todo momento de contacto con trombina, ya sea de 5 a 30 minutos, no hubo interferencia en la acción de trombina o fibrinógeno en la formación de trombos. Como solo se alteró el tiempo de protrombina entre todos los estudiados, se sugiere que la proteasa fibrinolítica en estudio afecta la vía extrínseca de la coagulación. Así, fue posible observar que la serina proteasa promovió una prolongación significativa del tiempo de coagulación de PT a medida que aumentaba la concentración de enzima, pero la serina proteasa no provocó ningún tipo de cambio en TT y APTT.As proteases fibrinolíticas são enzimas que degradam a fibrina, principal componente do coágulo sanguíneo. Assim, o presente estudo teve como objetivo compreender a avaliação do TTPa, TT e TP em plasma tratado com uma protease fibrinolítica produzida por Mucor subtillissimus. Para avaliar o efeito anticoagulante, a protease foi utilizada em diferentes concentrações de 0,5-2,5mg/mL. Os testes mostraram que a enzima promoveu um tempo prolongado significativo ao longo do tempo de coagulação do TP com o aumento da concentração. No ensaio de TTPa, a protease fibrinolítica praticamente não prolongou o tempo de coagulação, mesmo com o aumento da concentração da enzima. No TT verificou-se que em todos os momentos de contato com a trombina, seja de 5 a 30 minutos, não houve interferência na ação da trombina ou do fibrinogênio na formação de trombos. Como apenas o tempo de protrombina entre todos os estudados foi alterado, sugere-se que a protease fibrinolítica em estudo afeta a via extrínseca da coagulação. Assim, foi possível observar que a serina protease promoveu um prolongamento significativo do tempo de coagulação do PT conforme a concentração da enzima aumentou, mas a serina protease não causou nenhum tipo de alteração no TT e no TTPa.Research, Society and Development2022-01-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2543910.33448/rsd-v11i2.25439Research, Society and Development; Vol. 11 No. 2; e15311225439Research, Society and Development; Vol. 11 Núm. 2; e15311225439Research, Society and Development; v. 11 n. 2; e153112254392525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/25439/22407Copyright (c) 2022 Vanessa Maria Andrade Machado Miranda; José Pedro Martins Barbosa Filho; Romero Marcos Pedrosa Brandão Costa; Ana Cristina Lima Leite; Vagne de Melo Oliveira; Juanize Matias da Silva Batista; Lorenzo Pastrana; Thiago Pajeú Nascimento; Ana Lúcia Figueiredo Portohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessMiranda, Vanessa Maria Andrade Machado Barbosa Filho, José Pedro Martins Costa, Romero Marcos Pedrosa Brandão Leite, Ana Cristina Lima Oliveira, Vagne de Melo Batista, Juanize Matias da Silva Pastrana, LorenzoNascimento, Thiago Pajeú Porto, Ana Lúcia Figueiredo2022-02-07T01:42:50Zoai:ojs.pkp.sfu.ca:article/25439Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:43:42.945991Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Evaluation of partial thromboplastin time, thrombin time and prothrombin time over treated plasma using a fibrinolytic protease
Evaluación del tiempo de tromboplastina parcial, tiempo de trombina y tiempo de protrombina en plasma tratado con proteasa fibrinolítica
Avaliação do tempo parcial de tromboplastina, tempo de trombina e tempo de protrombina sobre o plasma tratado usando uma protease fibrinolítica
title Evaluation of partial thromboplastin time, thrombin time and prothrombin time over treated plasma using a fibrinolytic protease
spellingShingle Evaluation of partial thromboplastin time, thrombin time and prothrombin time over treated plasma using a fibrinolytic protease
Miranda, Vanessa Maria Andrade Machado
Trombose
Agente fibrinolítico
Coagulação
Plasma.
Trombosis
Fibrinolíticos
Coagulación
Plasma.
Thrombosis
Fibrinolytic agents
Coagulation
Plasma.
title_short Evaluation of partial thromboplastin time, thrombin time and prothrombin time over treated plasma using a fibrinolytic protease
title_full Evaluation of partial thromboplastin time, thrombin time and prothrombin time over treated plasma using a fibrinolytic protease
title_fullStr Evaluation of partial thromboplastin time, thrombin time and prothrombin time over treated plasma using a fibrinolytic protease
title_full_unstemmed Evaluation of partial thromboplastin time, thrombin time and prothrombin time over treated plasma using a fibrinolytic protease
title_sort Evaluation of partial thromboplastin time, thrombin time and prothrombin time over treated plasma using a fibrinolytic protease
author Miranda, Vanessa Maria Andrade Machado
author_facet Miranda, Vanessa Maria Andrade Machado
Barbosa Filho, José Pedro Martins
Costa, Romero Marcos Pedrosa Brandão
Leite, Ana Cristina Lima
Oliveira, Vagne de Melo
Batista, Juanize Matias da Silva
Pastrana, Lorenzo
Nascimento, Thiago Pajeú
Porto, Ana Lúcia Figueiredo
author_role author
author2 Barbosa Filho, José Pedro Martins
Costa, Romero Marcos Pedrosa Brandão
Leite, Ana Cristina Lima
Oliveira, Vagne de Melo
Batista, Juanize Matias da Silva
Pastrana, Lorenzo
Nascimento, Thiago Pajeú
Porto, Ana Lúcia Figueiredo
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Miranda, Vanessa Maria Andrade Machado
Barbosa Filho, José Pedro Martins
Costa, Romero Marcos Pedrosa Brandão
Leite, Ana Cristina Lima
Oliveira, Vagne de Melo
Batista, Juanize Matias da Silva
Pastrana, Lorenzo
Nascimento, Thiago Pajeú
Porto, Ana Lúcia Figueiredo
dc.subject.por.fl_str_mv Trombose
Agente fibrinolítico
Coagulação
Plasma.
Trombosis
Fibrinolíticos
Coagulación
Plasma.
Thrombosis
Fibrinolytic agents
Coagulation
Plasma.
topic Trombose
Agente fibrinolítico
Coagulação
Plasma.
Trombosis
Fibrinolíticos
Coagulación
Plasma.
Thrombosis
Fibrinolytic agents
Coagulation
Plasma.
description Fibrinolytic proteases are enzymes that degrade the fibrin, a main component of the blood clot. Thus, the present study aimed to understand the evaluation of aPTT, TT and PT on plasma treated with a fibrinolytic protease produced by Mucor subtillissimus. To assess the anticoagulant effect, the protease was used in different concentrations of 0.5-2.5mg/mL. The tests showed that the enzyme promoted a significant prolonged time over the PT clotting time as concentration increased. In the aPTT assay, fibrinolytic protease practically did not prolong the clotting time, even with increased enzyme concentration. In TT it was verified that at all times of contact with thrombin, be it 5-30 minutes, there was no interference on the action of thrombin or fibrinogen on the formation of thrombi. Since only the prothrombin time among all those studied was altered, it is suggested that the fibrinolytic protease under study affects the extrinsic pathway of coagulation. Thus, it was possible to observe that the serine protease promoted a significant prolonged time on the clotting time of the PT as the enzyme concentration increased, but the serine protease did not cause any type of change in TT and aPTT.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-21
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/25439
10.33448/rsd-v11i2.25439
url https://rsdjournal.org/index.php/rsd/article/view/25439
identifier_str_mv 10.33448/rsd-v11i2.25439
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/25439/22407
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 11 No. 2; e15311225439
Research, Society and Development; Vol. 11 Núm. 2; e15311225439
Research, Society and Development; v. 11 n. 2; e15311225439
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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