Animal development, liver histology, and antioxidant activity in the muscle of zebrafish (Danio rerio) fed with natural additives in the diets
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/27326 |
Resumo: | This work was carried out to evaluate the animal development, liver histology, and antioxidant effects on zebrafish fed with natural additives in different levels. Natural additives were composed by Baccharis dracunculifolia extract, cashew nut shell liquid (CNSL), and clove leaf essential oil (CLEO). The groups were: CONT: basal diet without the addition; CONB: addition of 50 g BHT kg-1 feed; MIX1: addition of 50 g of natural additives kg-1 feed (60% B. dracunculifolia, 39% CNSL, and 1% CLEO); MIX2: addition of 50 g of natural additives kg-1 feed (70% B. dracunculifolia, 29% CNSL, and 1% CLEO); MIX3: addition of 50 g of natural additives kg-1 feed (90% B. dracunculifolia, 9% CNSL, and 1% CLEO); and MIX4: addition of 50 g of natural additives kg-1 feed (99% B. dracunculifolia, 0.9% CNSL, and 0.1% CLEO). The average daily gain was lower for fishes fed the MIX4 diet. Diets effect was observed in liver histology (P < 0.001), which were greater in fishes fed CONB and MIX4 diets. Antioxidant activity in the diet evaluated using the ABTS assay was greater (P < 0.05) for treatments with natural additives. Similarly, antioxidant activity in zebrafish muscle (P < 0.05). Thus, the inclusion of 5% of natural additives in the diet composed by B. dracunculifolia extract, CNSL, and CLEO improved the antioxidant activity of zebrafish muscle. |
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Research, Society and Development |
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Animal development, liver histology, and antioxidant activity in the muscle of zebrafish (Danio rerio) fed with natural additives in the dietsDesarrollo animal, histología hepatica y actividade antioxidante en músculo de zebrafish (Danio rerio) alimentado con aditivos naturales en la dietaDesenvolvimento animal, histologia hepatica e atividade antioxidante do músculo de zebrafish (Danio rerio) alimentado com aditivos naturais na dieta ABTSAnimal productionDPPHNatural extractsPhytochemicals.ABTSDPPHExtractos naturalesFitoquímicosProducción animal.ABTSDPPHExtratos naturaisFitoquímicosProdução animal.This work was carried out to evaluate the animal development, liver histology, and antioxidant effects on zebrafish fed with natural additives in different levels. Natural additives were composed by Baccharis dracunculifolia extract, cashew nut shell liquid (CNSL), and clove leaf essential oil (CLEO). The groups were: CONT: basal diet without the addition; CONB: addition of 50 g BHT kg-1 feed; MIX1: addition of 50 g of natural additives kg-1 feed (60% B. dracunculifolia, 39% CNSL, and 1% CLEO); MIX2: addition of 50 g of natural additives kg-1 feed (70% B. dracunculifolia, 29% CNSL, and 1% CLEO); MIX3: addition of 50 g of natural additives kg-1 feed (90% B. dracunculifolia, 9% CNSL, and 1% CLEO); and MIX4: addition of 50 g of natural additives kg-1 feed (99% B. dracunculifolia, 0.9% CNSL, and 0.1% CLEO). The average daily gain was lower for fishes fed the MIX4 diet. Diets effect was observed in liver histology (P < 0.001), which were greater in fishes fed CONB and MIX4 diets. Antioxidant activity in the diet evaluated using the ABTS assay was greater (P < 0.05) for treatments with natural additives. Similarly, antioxidant activity in zebrafish muscle (P < 0.05). Thus, the inclusion of 5% of natural additives in the diet composed by B. dracunculifolia extract, CNSL, and CLEO improved the antioxidant activity of zebrafish muscle.Este trabajo se llevó a cabo para evaluar el crecimiento animal, la histología hepática y los efectos antioxidantes en zebrafish alimentados con aditivos naturales en diferentes niveles. Los aditivos naturales estaban compuestos por extracto de Baccharis dracunculifolia, líquido de cáscara de anacardo (CNSL) y aceite esencial de clavo (CLEO). Los grupos fueron: CONT: dieta basal sin adición; CONB: adición de 50 g BHT/kg de ración; MIX1: adición de 50 g de aditivos naturales/kg de ración (60% B. dracunculifolia, 39% CNSL y 1% CLEO); MIX2: adición de 50 g de aditivos naturales/kg de ración (70% B. dracunculifolia, 29% CNSL y 1% CLEO); MIX3: adición de 50 g de aditivos naturales/kg de ración (90% B. dracunculifolia, 9% CNSL y 1% CLEO); y MIX4: adición de 50 g de aditivos naturales/kg de ración (99% B. dracunculifolia, 0,9% CNSL y 0,1% CLEO). La ganancia diaria promedio fue menor para los peces alimentados con la dieta MIX4. El efecto de las dietas se observó en la histología del hígado (P < 0,001), que fue mayor en los peces alimentados con dietas CONB y MIX4. La actividad antioxidante en la dieta evaluada por el ensayo ABTS fue mayor (P < 0,05) para los tratamientos con aditivos naturales. Asimismo, actividad antioxidante en músculo de zebrafish (P < 0,05). Así, la inclusión de 5% de aditivos naturales en la dieta compuesta por extracto de B. dracunculifolia, CNSL y CLEO mejoró la actividad antioxidante del músculo de zebrafish.Este trabalho foi realizado para avaliar o crescimento animal, histologia hepática e efeitos antioxidantes em zebrafish alimentados com aditivos naturais em diferentes níveis. Os aditivos naturais foram compostos por extrato de Baccharis dracunculifolia, líquido da casca de castanha de caju (CNSL) e óleo essencial da folha do cravo da Índia (CLEO). Os grupos foram: CONT: dieta basal sem adição; CONB: adição de 50 g BHT/kg de ração; MIX1: adição de 50 g de aditivos naturais/kg de ração (60% B. dracunculifolia, 39% CNSL e 1% CLEO); MIX2: adição de 50 g de aditivos naturais/kg de ração (70% B. dracunculifolia, 29% CNSL e 1% CLEO); MIX3: adição de 50 g de aditivos naturais/kg de ração (90% B. dracunculifolia, 9% CNSL e 1% CLEO); e MIX4: adição de 50 g de aditivos naturais/kg de ração (99% B. dracunculifolia, 0,9% CNSL e 0,1% CLEO). O ganho médio diário foi menor para os peixes alimentados com a dieta MIX4. O efeito das dietas foi observado na histologia hepática (P < 0,001), que foi maior nos peixes alimentados com as dietas CONB e MIX4. A atividade antioxidante na dieta avaliada pelo ensaio ABTS foi maior (P < 0,05) para os tratamentos com aditivos naturais. Da mesma forma, a atividade antioxidante no músculo de zebrafish (P < 0,05). Assim, a inclusão de 5% de aditivos naturais na dieta composta por extrato de B. dracunculifolia, CNSL e CLEO melhorou a atividade antioxidante do músculo de zebrafish.Research, Society and Development2022-03-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2732610.33448/rsd-v11i4.27326Research, Society and Development; Vol. 11 No. 4; e41111427326Research, Society and Development; Vol. 11 Núm. 4; e41111427326Research, Society and Development; v. 11 n. 4; e411114273262525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/27326/24045Copyright (c) 2022 Tatiane Rogelio Ramos; Kennyson Alves Souza; Vanessa Lewandowski ; Rodolpho Martin do Prado; Mariana Garcia Ornaghi; Ana Carolina Pelaes Vital; Luiz Fernando de Souza Alves; Ricardo Pereira Ribeiro; Ivanor Nunes Pradohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessRamos, Tatiane Rogelio Souza, Kennyson AlvesLewandowski , VanessaPrado, Rodolpho Martin do Ornaghi, Mariana Garcia Vital, Ana Carolina PelaesAlves, Luiz Fernando de SouzaRibeiro, Ricardo Pereira Prado, Ivanor Nunes2022-03-27T17:17:09Zoai:ojs.pkp.sfu.ca:article/27326Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:45:06.109996Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Animal development, liver histology, and antioxidant activity in the muscle of zebrafish (Danio rerio) fed with natural additives in the diets Desarrollo animal, histología hepatica y actividade antioxidante en músculo de zebrafish (Danio rerio) alimentado con aditivos naturales en la dieta Desenvolvimento animal, histologia hepatica e atividade antioxidante do músculo de zebrafish (Danio rerio) alimentado com aditivos naturais na dieta |
title |
Animal development, liver histology, and antioxidant activity in the muscle of zebrafish (Danio rerio) fed with natural additives in the diets |
spellingShingle |
Animal development, liver histology, and antioxidant activity in the muscle of zebrafish (Danio rerio) fed with natural additives in the diets Ramos, Tatiane Rogelio ABTS Animal production DPPH Natural extracts Phytochemicals. ABTS DPPH Extractos naturales Fitoquímicos Producción animal. ABTS DPPH Extratos naturais Fitoquímicos Produção animal. |
title_short |
Animal development, liver histology, and antioxidant activity in the muscle of zebrafish (Danio rerio) fed with natural additives in the diets |
title_full |
Animal development, liver histology, and antioxidant activity in the muscle of zebrafish (Danio rerio) fed with natural additives in the diets |
title_fullStr |
Animal development, liver histology, and antioxidant activity in the muscle of zebrafish (Danio rerio) fed with natural additives in the diets |
title_full_unstemmed |
Animal development, liver histology, and antioxidant activity in the muscle of zebrafish (Danio rerio) fed with natural additives in the diets |
title_sort |
Animal development, liver histology, and antioxidant activity in the muscle of zebrafish (Danio rerio) fed with natural additives in the diets |
author |
Ramos, Tatiane Rogelio |
author_facet |
Ramos, Tatiane Rogelio Souza, Kennyson Alves Lewandowski , Vanessa Prado, Rodolpho Martin do Ornaghi, Mariana Garcia Vital, Ana Carolina Pelaes Alves, Luiz Fernando de Souza Ribeiro, Ricardo Pereira Prado, Ivanor Nunes |
author_role |
author |
author2 |
Souza, Kennyson Alves Lewandowski , Vanessa Prado, Rodolpho Martin do Ornaghi, Mariana Garcia Vital, Ana Carolina Pelaes Alves, Luiz Fernando de Souza Ribeiro, Ricardo Pereira Prado, Ivanor Nunes |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Ramos, Tatiane Rogelio Souza, Kennyson Alves Lewandowski , Vanessa Prado, Rodolpho Martin do Ornaghi, Mariana Garcia Vital, Ana Carolina Pelaes Alves, Luiz Fernando de Souza Ribeiro, Ricardo Pereira Prado, Ivanor Nunes |
dc.subject.por.fl_str_mv |
ABTS Animal production DPPH Natural extracts Phytochemicals. ABTS DPPH Extractos naturales Fitoquímicos Producción animal. ABTS DPPH Extratos naturais Fitoquímicos Produção animal. |
topic |
ABTS Animal production DPPH Natural extracts Phytochemicals. ABTS DPPH Extractos naturales Fitoquímicos Producción animal. ABTS DPPH Extratos naturais Fitoquímicos Produção animal. |
description |
This work was carried out to evaluate the animal development, liver histology, and antioxidant effects on zebrafish fed with natural additives in different levels. Natural additives were composed by Baccharis dracunculifolia extract, cashew nut shell liquid (CNSL), and clove leaf essential oil (CLEO). The groups were: CONT: basal diet without the addition; CONB: addition of 50 g BHT kg-1 feed; MIX1: addition of 50 g of natural additives kg-1 feed (60% B. dracunculifolia, 39% CNSL, and 1% CLEO); MIX2: addition of 50 g of natural additives kg-1 feed (70% B. dracunculifolia, 29% CNSL, and 1% CLEO); MIX3: addition of 50 g of natural additives kg-1 feed (90% B. dracunculifolia, 9% CNSL, and 1% CLEO); and MIX4: addition of 50 g of natural additives kg-1 feed (99% B. dracunculifolia, 0.9% CNSL, and 0.1% CLEO). The average daily gain was lower for fishes fed the MIX4 diet. Diets effect was observed in liver histology (P < 0.001), which were greater in fishes fed CONB and MIX4 diets. Antioxidant activity in the diet evaluated using the ABTS assay was greater (P < 0.05) for treatments with natural additives. Similarly, antioxidant activity in zebrafish muscle (P < 0.05). Thus, the inclusion of 5% of natural additives in the diet composed by B. dracunculifolia extract, CNSL, and CLEO improved the antioxidant activity of zebrafish muscle. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-03-22 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/27326 10.33448/rsd-v11i4.27326 |
url |
https://rsdjournal.org/index.php/rsd/article/view/27326 |
identifier_str_mv |
10.33448/rsd-v11i4.27326 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/27326/24045 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 11 No. 4; e41111427326 Research, Society and Development; Vol. 11 Núm. 4; e41111427326 Research, Society and Development; v. 11 n. 4; e41111427326 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
_version_ |
1797052707499933696 |