Resposta antioxidante enzimática, respiratória e fisiológica do tomate-cereja (Solanum lycopersicum var. cerasiforme) submetido ao choque térmico

Detalhes bibliográficos
Autor(a) principal: Paulus, Cristiane
Data de Publicação: 2016
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações do UNIOESTE
Texto Completo: http://tede.unioeste.br:8080/tede/handle/tede/1299
Resumo: Post-harvest treatments with thermal shock have been studied as an extension of alternative technical life of fruit. The beneficial effect of this technique has been related to their effects on the induction of physiological responses in protection against oxidative stress and development of pathogens. Enzymes are catalysts of the reactions occurring in biological systems. However, although the mechanisms by which post-harvest treatments induce this type of response are known in the plant organs are not clearly elucidated the mechanisms induced by postharvest heat shock that may affect the antioxidant status of treated fruits. The objective of this study was to evaluate the effect of heat shock on postharvest cherry tomatoes conservation, mediated biochemical and physicochemical answers related to antioxidant enzyme activity, respiratory activity, phenolic compounds, ascorbic, soluble solids acid, titratable acidity , percentage of weight, firmness, skin color and degradation of the fruit. The cherry tomatoes were subjected to heat shock treatments immersed in hot water at 45 ± 2 ° C at times 0, 5, 10, 15, 20 and 25 minutes. After treatments, fruits were divided into two groups. The first group was stored at 20 ± 2 ° C and at intervals of 1, 3, 6, 9 and 12 days, samples were taken and subjected to color analysis, firmness, weight loss, total phenolics, total flavonoids acid ascorbic acid and total soluble solids. The second group was submitted to respiratory activity assessments, ethylene production and enzymatic activity of superoxide dismutase, catalase and ascorbate peroxidase, at time intervals of 0, 2, 6, 24 and 48 hours of storage at 20 ± 2 ° C. According to the results, the fruits treated with heat shock suffered greater respiratory stress from the sixth day of storage. There was no significant difference between treatments for firmness, maintaining the rigidity of the fruit even after 12 days, and all treatments exhibited fruits with greater weight loss compared to the control. The application of heat treatment did not alter the total soluble solids content to the 6th day, heat exposure times of 15 and 20 min had a greater effect on the content of phenolic compounds during storage. exposed to heat fruits expressed the higher flavonoid content than the control and showed no recovery or increase in the concentration of ascorbic acid of cherry tomatoes in response to heat shock treatments that could indicate suppressive effect to stress. Thus, despite having the ability to prolong the life of cherry tomato, reducing the loss of the fruit after storage was not favorable for their use to reduce costs to prolong their shelf life
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spelling Braga, Gilberto Costahttp://lattes.cnpq.br/5595085713178176Kuhn, Odair Joséhttp://lattes.cnpq.br/0333372790090109Viecelli, Clair Aparecidahttp://lattes.cnpq.br/0219320686223781http://lattes.cnpq.br/4093702796565050Paulus, Cristiane2017-07-10T17:37:12Z2017-01-132016-01-29PAULUS, Cristiane. Resposta antioxidante enzimática, respiratória e fisiológica do tomate-cereja (Solanum lycopersicum var. cerasiforme) submetido ao choque térmico. 2016. 59 f. Dissertação (Mestrado em Agronomia) - Universidade Estadual do Oeste do Paraná, Marechal Cândido Rondon, 2016.http://tede.unioeste.br:8080/tede/handle/tede/1299Post-harvest treatments with thermal shock have been studied as an extension of alternative technical life of fruit. The beneficial effect of this technique has been related to their effects on the induction of physiological responses in protection against oxidative stress and development of pathogens. Enzymes are catalysts of the reactions occurring in biological systems. However, although the mechanisms by which post-harvest treatments induce this type of response are known in the plant organs are not clearly elucidated the mechanisms induced by postharvest heat shock that may affect the antioxidant status of treated fruits. The objective of this study was to evaluate the effect of heat shock on postharvest cherry tomatoes conservation, mediated biochemical and physicochemical answers related to antioxidant enzyme activity, respiratory activity, phenolic compounds, ascorbic, soluble solids acid, titratable acidity , percentage of weight, firmness, skin color and degradation of the fruit. The cherry tomatoes were subjected to heat shock treatments immersed in hot water at 45 ± 2 ° C at times 0, 5, 10, 15, 20 and 25 minutes. After treatments, fruits were divided into two groups. The first group was stored at 20 ± 2 ° C and at intervals of 1, 3, 6, 9 and 12 days, samples were taken and subjected to color analysis, firmness, weight loss, total phenolics, total flavonoids acid ascorbic acid and total soluble solids. The second group was submitted to respiratory activity assessments, ethylene production and enzymatic activity of superoxide dismutase, catalase and ascorbate peroxidase, at time intervals of 0, 2, 6, 24 and 48 hours of storage at 20 ± 2 ° C. According to the results, the fruits treated with heat shock suffered greater respiratory stress from the sixth day of storage. There was no significant difference between treatments for firmness, maintaining the rigidity of the fruit even after 12 days, and all treatments exhibited fruits with greater weight loss compared to the control. The application of heat treatment did not alter the total soluble solids content to the 6th day, heat exposure times of 15 and 20 min had a greater effect on the content of phenolic compounds during storage. exposed to heat fruits expressed the higher flavonoid content than the control and showed no recovery or increase in the concentration of ascorbic acid of cherry tomatoes in response to heat shock treatments that could indicate suppressive effect to stress. Thus, despite having the ability to prolong the life of cherry tomato, reducing the loss of the fruit after storage was not favorable for their use to reduce costs to prolong their shelf lifeTratamentos pós-colheita com choque térmico têm sido estudado como técnica alternativa de extensão da vida útil de frutos. A ação benéfica dessa técnica tem sido relacionada com seus efeitos na indução de respostas fisiológicas de defesa contra estresses oxidativos e desenvolvimento de patógenos. As enzimas são os catalisadores das reações que ocorrem nos sistemas biológicos. Entretanto, embora sejam conhecidos os mecanismos pelos quais os tratamentos pós-colheita induzem este tipo de resposta nos órgãos vegetais, ainda não estão claramente elucidados os mecanismos induzidos pelo choque térmico pós-colheita que possam afetar o status antioxidante de frutos tratados. O objetivo deste trabalho foi avaliar o efeito do choque térmico na conservação pós-colheita de tomates-cereja, mediada por respostas bioquímicas e físico-químicas relacionadas à atividade enzimática antioxidante, atividade respiratória, compostos fenólicos, ácido ascórbico, sólidos solúveis, acidez total titulável, porcentagem de massa fresca, firmeza, cor da casca e degradação dos frutos. Os tomates-cereja foram submetidos aos tratamentos de choque térmico com imersão em água quente a 45 ± 2 ºC nos tempos de 0, 5, 10, 15, 20 e 25 minutos. Após os tratamentos, os frutos foram divididos em dois grupos. O primeiro grupo foi armazenado a 20 ± 2 ºC e, em intervalos de 1, 3, 6, 9 e 12 dias, amostras foram retiradas e submetidas a análises de cor, firmeza, perda de massa, compostos fenólicos totais, flavonoides totais, ácido ascórbico e sólidos solúveis totais. O segundo grupo foi submetido às avaliações de atividade respiratória, produção de etileno e atividade enzimática de superóxido dismutase, catalase e ascorbato peroxidase, nos intervalos de tempo de 0, 2, 6, 24 e 48 horas de armazenamento à 20 ± 2 ºC. De acordo com os resultados, os frutos tratados com o choque térmico sofreram maior estresse respiratório a partir do sexto dia de armazenamento. Não houve diferença significativa entre os tratamentos para a firmeza, permanecendo a rigidez do fruto mesmo após 12º dias, e todos os tratamentos exibiram frutos com maior perda de massa, quando comparado ao controle. A aplicação dos tratamentos térmicos não alterou o teor de sólidos solúveis totais até o 6º dia, os tempos de exposição ao calor de 15 e 20 min tiveram maior efeito nos conteúdos de compostos fenólicos ao longo do armazenamento. Os frutos expostos ao calor expressaram conteúdos de flavonoides mais elevados do que o controle e não mostraram recuperação ou aumento na concentração de ácido ascórbico dos tomates-cereja em resposta aos tratamentos de choque térmico que pudessem indicar efeito supressor ao estresse. Com isso, apesar de possuir a capacidade de prolongar a vida útil do tomate-cereja, reduzindo a perda do fruto após o armazenamento, não se mostrou favorável para a sua utilização de forma a reduzir gastos para prolongar seu tempo de prateleiraMade available in DSpace on 2017-07-10T17:37:12Z (GMT). No. of bitstreams: 1 Cristiane Paulus.pdf: 907945 bytes, checksum: f84d9e19a342d1e11d80efa3864b0389 (MD5) Previous issue date: 2016-01-29Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfpor-6392337873870130111500Universidade Estadual do Oeste do ParanáMarechal Cândido RondonPrograma de Pós-Graduação em AgronomiaUNIOESTEBRCentro de Ciências AgráriasTomate-cerejaCalorSuperóxido dismutaseCatalaseAscorbato peroxidaseCherry tomatoHeatSuperoxide dismutaseCatalaseAscorbateCIÊNCIAS AGRÁRIAS:AGRONOMIAResposta antioxidante enzimática, respiratória e fisiológica do tomate-cereja (Solanum lycopersicum var. cerasiforme) submetido ao choque térmicoAntioxidant enzyme answer, respiratory and physiological of tomato-cherry (Solanum lycopersicum var. cerasiforme) submitted When thermal shockinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UNIOESTEinstname:Universidade Estadual do Oeste do Paraná (UNIOESTE)instacron:UNIOESTEORIGINALCristiane_Paulus_2016application/pdf907945http://tede.unioeste.br:8080/tede/bitstream/tede/1299/1/Cristiane_Paulus_2016f84d9e19a342d1e11d80efa3864b0389MD51tede/12992017-10-19 20:50:37.996oai:tede.unioeste.br:tede/1299Biblioteca Digital de Teses e Dissertaçõeshttp://tede.unioeste.br/PUBhttp://tede.unioeste.br/oai/requestbiblioteca.repositorio@unioeste.bropendoar:2017-10-19T22:50:37Biblioteca Digital de Teses e Dissertações do UNIOESTE - Universidade Estadual do Oeste do Paraná (UNIOESTE)false
dc.title.por.fl_str_mv Resposta antioxidante enzimática, respiratória e fisiológica do tomate-cereja (Solanum lycopersicum var. cerasiforme) submetido ao choque térmico
dc.title.alternative.eng.fl_str_mv Antioxidant enzyme answer, respiratory and physiological of tomato-cherry (Solanum lycopersicum var. cerasiforme) submitted When thermal shock
title Resposta antioxidante enzimática, respiratória e fisiológica do tomate-cereja (Solanum lycopersicum var. cerasiforme) submetido ao choque térmico
spellingShingle Resposta antioxidante enzimática, respiratória e fisiológica do tomate-cereja (Solanum lycopersicum var. cerasiforme) submetido ao choque térmico
Paulus, Cristiane
Tomate-cereja
Calor
Superóxido dismutase
Catalase
Ascorbato peroxidase
Cherry tomato
Heat
Superoxide dismutase
Catalase
Ascorbate
CIÊNCIAS AGRÁRIAS:AGRONOMIA
title_short Resposta antioxidante enzimática, respiratória e fisiológica do tomate-cereja (Solanum lycopersicum var. cerasiforme) submetido ao choque térmico
title_full Resposta antioxidante enzimática, respiratória e fisiológica do tomate-cereja (Solanum lycopersicum var. cerasiforme) submetido ao choque térmico
title_fullStr Resposta antioxidante enzimática, respiratória e fisiológica do tomate-cereja (Solanum lycopersicum var. cerasiforme) submetido ao choque térmico
title_full_unstemmed Resposta antioxidante enzimática, respiratória e fisiológica do tomate-cereja (Solanum lycopersicum var. cerasiforme) submetido ao choque térmico
title_sort Resposta antioxidante enzimática, respiratória e fisiológica do tomate-cereja (Solanum lycopersicum var. cerasiforme) submetido ao choque térmico
author Paulus, Cristiane
author_facet Paulus, Cristiane
author_role author
dc.contributor.advisor1.fl_str_mv Braga, Gilberto Costa
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/5595085713178176
dc.contributor.referee1.fl_str_mv Kuhn, Odair José
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/0333372790090109
dc.contributor.referee2.fl_str_mv Viecelli, Clair Aparecida
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/0219320686223781
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/4093702796565050
dc.contributor.author.fl_str_mv Paulus, Cristiane
contributor_str_mv Braga, Gilberto Costa
Kuhn, Odair José
Viecelli, Clair Aparecida
dc.subject.por.fl_str_mv Tomate-cereja
Calor
Superóxido dismutase
Catalase
Ascorbato peroxidase
topic Tomate-cereja
Calor
Superóxido dismutase
Catalase
Ascorbato peroxidase
Cherry tomato
Heat
Superoxide dismutase
Catalase
Ascorbate
CIÊNCIAS AGRÁRIAS:AGRONOMIA
dc.subject.eng.fl_str_mv Cherry tomato
Heat
Superoxide dismutase
Catalase
Ascorbate
dc.subject.cnpq.fl_str_mv CIÊNCIAS AGRÁRIAS:AGRONOMIA
description Post-harvest treatments with thermal shock have been studied as an extension of alternative technical life of fruit. The beneficial effect of this technique has been related to their effects on the induction of physiological responses in protection against oxidative stress and development of pathogens. Enzymes are catalysts of the reactions occurring in biological systems. However, although the mechanisms by which post-harvest treatments induce this type of response are known in the plant organs are not clearly elucidated the mechanisms induced by postharvest heat shock that may affect the antioxidant status of treated fruits. The objective of this study was to evaluate the effect of heat shock on postharvest cherry tomatoes conservation, mediated biochemical and physicochemical answers related to antioxidant enzyme activity, respiratory activity, phenolic compounds, ascorbic, soluble solids acid, titratable acidity , percentage of weight, firmness, skin color and degradation of the fruit. The cherry tomatoes were subjected to heat shock treatments immersed in hot water at 45 ± 2 ° C at times 0, 5, 10, 15, 20 and 25 minutes. After treatments, fruits were divided into two groups. The first group was stored at 20 ± 2 ° C and at intervals of 1, 3, 6, 9 and 12 days, samples were taken and subjected to color analysis, firmness, weight loss, total phenolics, total flavonoids acid ascorbic acid and total soluble solids. The second group was submitted to respiratory activity assessments, ethylene production and enzymatic activity of superoxide dismutase, catalase and ascorbate peroxidase, at time intervals of 0, 2, 6, 24 and 48 hours of storage at 20 ± 2 ° C. According to the results, the fruits treated with heat shock suffered greater respiratory stress from the sixth day of storage. There was no significant difference between treatments for firmness, maintaining the rigidity of the fruit even after 12 days, and all treatments exhibited fruits with greater weight loss compared to the control. The application of heat treatment did not alter the total soluble solids content to the 6th day, heat exposure times of 15 and 20 min had a greater effect on the content of phenolic compounds during storage. exposed to heat fruits expressed the higher flavonoid content than the control and showed no recovery or increase in the concentration of ascorbic acid of cherry tomatoes in response to heat shock treatments that could indicate suppressive effect to stress. Thus, despite having the ability to prolong the life of cherry tomato, reducing the loss of the fruit after storage was not favorable for their use to reduce costs to prolong their shelf life
publishDate 2016
dc.date.issued.fl_str_mv 2016-01-29
dc.date.accessioned.fl_str_mv 2017-07-10T17:37:12Z
dc.date.available.fl_str_mv 2017-01-13
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv PAULUS, Cristiane. Resposta antioxidante enzimática, respiratória e fisiológica do tomate-cereja (Solanum lycopersicum var. cerasiforme) submetido ao choque térmico. 2016. 59 f. Dissertação (Mestrado em Agronomia) - Universidade Estadual do Oeste do Paraná, Marechal Cândido Rondon, 2016.
dc.identifier.uri.fl_str_mv http://tede.unioeste.br:8080/tede/handle/tede/1299
identifier_str_mv PAULUS, Cristiane. Resposta antioxidante enzimática, respiratória e fisiológica do tomate-cereja (Solanum lycopersicum var. cerasiforme) submetido ao choque térmico. 2016. 59 f. Dissertação (Mestrado em Agronomia) - Universidade Estadual do Oeste do Paraná, Marechal Cândido Rondon, 2016.
url http://tede.unioeste.br:8080/tede/handle/tede/1299
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dc.publisher.none.fl_str_mv Universidade Estadual do Oeste do Paraná
Marechal Cândido Rondon
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Agronomia
dc.publisher.initials.fl_str_mv UNIOESTE
dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Centro de Ciências Agrárias
publisher.none.fl_str_mv Universidade Estadual do Oeste do Paraná
Marechal Cândido Rondon
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