A participação esplênica e vagal na deposição de lipídeos no tecido adiposo marrom de ratos obesos e não obesos

Detalhes bibliográficos
Autor(a) principal: Nottar, Cassiane Lisbinski
Data de Publicação: 2022
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações do UNIOESTE
Texto Completo: https://tede.unioeste.br/handle/tede/6036
Resumo: Brown Adipose Tissue (BAT) is specialized in thermogenesis, a process that involves the degradation of energetic substrates, especially lipids, and the formation of a mitochondrial electrochemical gradient that is dissipated by the mitochondrial uncoupling protein (UCP-1) in the form of heat. Classically, thermogenesis is stimulated by the activity of the Sympathetic Nervous System (SNS), which is reduced in obesity. Studies indicate that the parasympathetic branch, via the Vagus Nerve (NV) is able to influence thermogenesis in BAT. Obese people have vagal hyperactivity. NV also modulates the immune response, and its anti-inflammatory effects depend on the presence of the spleen. In the present study, the histological parameters of BAT in obese and non-obese rodents undergoing vagotomy associated with splenectomy were evaluated. For this, obesity was induced by the subcutaneous injection of monosodium glutamate (M; 4g/Kg) in the first postnatal week. Control (CTL) or nonobese rats received equimolar saline. M and CTL were weaned at 21 days, and at 60 days of life they were randomly assigned to groups according to surgery: Splenectomy (spl) – removal of the spleen; vagotomy (vag) – NV section; spl+vag (both surgeries) and false-operated (sham) – surgical stress simulation (n=6 rats/group). At 150 days of age and after 12 hours of fasting, the animals were weighed and euthanized, and the BAT of the interscapular region was removed, cleaned, washed and weighed. Part of the TAM was transferred to a fixative and submitted to histological technique and staining with hematoxylin and eosin (HE). Data were expressed as mean±SEM, using the Student's t test or two-way ANOVA with Tukey's posttest (p<0.05). M rats are smaller, with a higher BAT weight, in which there is a higher lipid content compared to CTL animals (p<0.05). In the CTL group, vag reduces body weight, BAT weight and fat deposition n in relation to sham. Spl reduces the number of nuclei and increase fat deposition in the BAT of CTL animals. On the other hand, in M-obese patients, vag does not significantly alter any parameter of the SAT, while spl increases the number of nuclei. Thus, the present study indicates that NV modulates the lipid content in the BAT of non-obese rats, without splenic interference. In obese subjects, however, the effect of NV seems to be absent, while the spleen, through an unknown route, influences the BAT of obese rats.
id UNIOESTE-1_757aa846d20f64a86e804a451cbf3c10
oai_identifier_str oai:tede.unioeste.br:tede/6036
network_acronym_str UNIOESTE-1
network_name_str Biblioteca Digital de Teses e Dissertações do UNIOESTE
repository_id_str
spelling Grassiolli, Sabrinahttp://lattes.cnpq.br/1379417550389891Grassiolli, Sabrinahttp://lattes.cnpq.br/1379417550389891Pascotto, Claudicéia Rissohttp://lattes.cnpq.br/9474794343913304Oliveira, Julio Cezar dehttp://lattes.cnpq.br/0189760778239540http://lattes.cnpq.br/8380631497090282Nottar, Cassiane Lisbinski2022-05-13T12:50:22Z2022-03-08NOTTAR, Cassiane Lisbinski. A participação esplênica e vagal na deposição de lipídeos no tecido adiposo marrom de ratos obesos e não obesos. 2022. 65f. Dissertação (Mestrado em Ciências Aplicadas à Saúde) - Universidade Estadual do Oeste do Paraná, Francisco Beltrão, 2022.https://tede.unioeste.br/handle/tede/6036Brown Adipose Tissue (BAT) is specialized in thermogenesis, a process that involves the degradation of energetic substrates, especially lipids, and the formation of a mitochondrial electrochemical gradient that is dissipated by the mitochondrial uncoupling protein (UCP-1) in the form of heat. Classically, thermogenesis is stimulated by the activity of the Sympathetic Nervous System (SNS), which is reduced in obesity. Studies indicate that the parasympathetic branch, via the Vagus Nerve (NV) is able to influence thermogenesis in BAT. Obese people have vagal hyperactivity. NV also modulates the immune response, and its anti-inflammatory effects depend on the presence of the spleen. In the present study, the histological parameters of BAT in obese and non-obese rodents undergoing vagotomy associated with splenectomy were evaluated. For this, obesity was induced by the subcutaneous injection of monosodium glutamate (M; 4g/Kg) in the first postnatal week. Control (CTL) or nonobese rats received equimolar saline. M and CTL were weaned at 21 days, and at 60 days of life they were randomly assigned to groups according to surgery: Splenectomy (spl) – removal of the spleen; vagotomy (vag) – NV section; spl+vag (both surgeries) and false-operated (sham) – surgical stress simulation (n=6 rats/group). At 150 days of age and after 12 hours of fasting, the animals were weighed and euthanized, and the BAT of the interscapular region was removed, cleaned, washed and weighed. Part of the TAM was transferred to a fixative and submitted to histological technique and staining with hematoxylin and eosin (HE). Data were expressed as mean±SEM, using the Student's t test or two-way ANOVA with Tukey's posttest (p<0.05). M rats are smaller, with a higher BAT weight, in which there is a higher lipid content compared to CTL animals (p<0.05). In the CTL group, vag reduces body weight, BAT weight and fat deposition n in relation to sham. Spl reduces the number of nuclei and increase fat deposition in the BAT of CTL animals. On the other hand, in M-obese patients, vag does not significantly alter any parameter of the SAT, while spl increases the number of nuclei. Thus, the present study indicates that NV modulates the lipid content in the BAT of non-obese rats, without splenic interference. In obese subjects, however, the effect of NV seems to be absent, while the spleen, through an unknown route, influences the BAT of obese rats.O Tecido Adiposo Marrom (TAM) é especializado na termogênese, um processo que envolve a degradação de substratos energéticos, em especial os lipídios e a formação de um gradiente eletroquímico mitocondrial que é dissipado pela proteína desacopladora mitocondrial (UCP1) na forma de calor. Classicamente a termogênese é estimulada pela atividade do Sistema Nervoso Simpático (SNS), o qual está reduzido na obesidade. Estudos apontam que o ramo parassimpático, via Nervo Vago (NV) é capaz de influenciar a termogênese no TAM. Obesos tem hiperatividade vagal. O NV também modula a resposta imunológica, e seus efeitos anti-inflamatórios dependem da presença do baço. No presente estudo foram avaliados os parâmetros histológicos do TAM em roedores obesos e não-obesos submetidos a vagotomia associado a esplenectomia. Para tal, a obesidade foi induzida pela injeção subcutânea de glutamato monossódico (M; 4g/Kg) na primeira semana pós-natal. Ratos controles (CTL) ou não-obesos receberam solução salina equimolar. Ratos M e CTL foram desmamados aos 21 dias, e aos 60 dias de vida randomicamente distribuídos nos grupos conforme cirurgia: Esplenectomia (spl) – retirada do baço; vagotomia (vag) – secção NV; spl+vag (ambas as cirurgias) e falso-operados (sham) – simulação estresse cirúrgico (n=6 – 8 ratos/grupo). Aos 150 dias de vida e após 12h de jejum, os animais foram pesados e eutanasiados, e o TAM da região interescapular foi retirado, limpo lavado e pesado. Parte do TAM foi transferida para fixador e submetida a técnica histológica e coloração com hematoxilina e eosina (HE). Dados foram expressos como média±EPM, sendo aplicados o teste t Student´s ou ANOVA de duas vias com pós teste de Tukey (p<0,05). Ratos M são obesos, apresentando maior peso do TAM, no qual há maior teor de lipídios em relação aos animais CTL (p<0.05). No grupo CTL a vag reduziu o peso corporal, o peso do TAM e a deposição de gordura em relação aos sham. A spl reduz o número de núcleos e eleva a deposição de gordura no TAM de animais CTL. Por outro lado, nos M-obesos a vag não altera significativamente nenhum parâmetro do TAM, enquanto a spl eleva o número núcleos. Assim o presente estudo indica que NV modula o teor de lipídios no TAM de ratos não-obesos, sem interferência esplênica. Nos obesos, entretanto, o efeito do NV parece estar ausente, enquanto o baço, por via ainda não conhecida influencia o TAM de ratos obesos.Submitted by Almir Squinsani (almir.squinsani@unioeste.br) on 2022-05-13T12:50:22Z No. of bitstreams: 2 Cassiane_Nottar_2022.pdf: 1391422 bytes, checksum: 6d3ab12138e5cfab4a52004d23d79979 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2022-05-13T12:50:22Z (GMT). No. of bitstreams: 2 Cassiane_Nottar_2022.pdf: 1391422 bytes, checksum: 6d3ab12138e5cfab4a52004d23d79979 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2022-03-08application/pdfpor-5356284425524309716500Universidade Estadual do Oeste do ParanáFrancisco BeltrãoPrograma de Pós-Graduação em Ciências Aplicadas à SaúdeUNIOESTEBrasilCentro de Ciências da Saúdehttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessTermogêneseLipídiosNervo vagoBaçoObesidadeThermogenesisLipidsVagus nerveSpleenObesityCIÊNCIAS DA SAÚDEA participação esplênica e vagal na deposição de lipídeos no tecido adiposo marrom de ratos obesos e não obesosSplenic and vagal participation in lipid deposition in brown adipose tissue of obese and non-obese ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis6290525253230630664600600292441653440865123reponame:Biblioteca Digital de Teses e Dissertações do UNIOESTEinstname:Universidade Estadual do Oeste do Paraná (UNIOESTE)instacron:UNIOESTEORIGINALCassiane_Nottar_2022.pdfCassiane_Nottar_2022.pdfapplication/pdf1391422http://tede.unioeste.br:8080/tede/bitstream/tede/6036/5/Cassiane_Nottar_2022.pdf6d3ab12138e5cfab4a52004d23d79979MD55CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://tede.unioeste.br:8080/tede/bitstream/tede/6036/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-80http://tede.unioeste.br:8080/tede/bitstream/tede/6036/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-80http://tede.unioeste.br:8080/tede/bitstream/tede/6036/4/license_rdfd41d8cd98f00b204e9800998ecf8427eMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://tede.unioeste.br:8080/tede/bitstream/tede/6036/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/60362022-05-13 09:50:22.601oai:tede.unioeste.br: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede.unioeste.br/PUBhttp://tede.unioeste.br/oai/requestbiblioteca.repositorio@unioeste.bropendoar:2022-05-13T12:50:22Biblioteca Digital de Teses e Dissertações do UNIOESTE - Universidade Estadual do Oeste do Paraná (UNIOESTE)false
dc.title.por.fl_str_mv A participação esplênica e vagal na deposição de lipídeos no tecido adiposo marrom de ratos obesos e não obesos
dc.title.alternative.eng.fl_str_mv Splenic and vagal participation in lipid deposition in brown adipose tissue of obese and non-obese rats
title A participação esplênica e vagal na deposição de lipídeos no tecido adiposo marrom de ratos obesos e não obesos
spellingShingle A participação esplênica e vagal na deposição de lipídeos no tecido adiposo marrom de ratos obesos e não obesos
Nottar, Cassiane Lisbinski
Termogênese
Lipídios
Nervo vago
Baço
Obesidade
Thermogenesis
Lipids
Vagus nerve
Spleen
Obesity
CIÊNCIAS DA SAÚDE
title_short A participação esplênica e vagal na deposição de lipídeos no tecido adiposo marrom de ratos obesos e não obesos
title_full A participação esplênica e vagal na deposição de lipídeos no tecido adiposo marrom de ratos obesos e não obesos
title_fullStr A participação esplênica e vagal na deposição de lipídeos no tecido adiposo marrom de ratos obesos e não obesos
title_full_unstemmed A participação esplênica e vagal na deposição de lipídeos no tecido adiposo marrom de ratos obesos e não obesos
title_sort A participação esplênica e vagal na deposição de lipídeos no tecido adiposo marrom de ratos obesos e não obesos
author Nottar, Cassiane Lisbinski
author_facet Nottar, Cassiane Lisbinski
author_role author
dc.contributor.advisor1.fl_str_mv Grassiolli, Sabrina
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/1379417550389891
dc.contributor.referee1.fl_str_mv Grassiolli, Sabrina
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/1379417550389891
dc.contributor.referee2.fl_str_mv Pascotto, Claudicéia Risso
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/9474794343913304
dc.contributor.referee3.fl_str_mv Oliveira, Julio Cezar de
dc.contributor.referee3Lattes.fl_str_mv http://lattes.cnpq.br/0189760778239540
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/8380631497090282
dc.contributor.author.fl_str_mv Nottar, Cassiane Lisbinski
contributor_str_mv Grassiolli, Sabrina
Grassiolli, Sabrina
Pascotto, Claudicéia Risso
Oliveira, Julio Cezar de
dc.subject.por.fl_str_mv Termogênese
Lipídios
Nervo vago
Baço
Obesidade
topic Termogênese
Lipídios
Nervo vago
Baço
Obesidade
Thermogenesis
Lipids
Vagus nerve
Spleen
Obesity
CIÊNCIAS DA SAÚDE
dc.subject.eng.fl_str_mv Thermogenesis
Lipids
Vagus nerve
Spleen
Obesity
dc.subject.cnpq.fl_str_mv CIÊNCIAS DA SAÚDE
description Brown Adipose Tissue (BAT) is specialized in thermogenesis, a process that involves the degradation of energetic substrates, especially lipids, and the formation of a mitochondrial electrochemical gradient that is dissipated by the mitochondrial uncoupling protein (UCP-1) in the form of heat. Classically, thermogenesis is stimulated by the activity of the Sympathetic Nervous System (SNS), which is reduced in obesity. Studies indicate that the parasympathetic branch, via the Vagus Nerve (NV) is able to influence thermogenesis in BAT. Obese people have vagal hyperactivity. NV also modulates the immune response, and its anti-inflammatory effects depend on the presence of the spleen. In the present study, the histological parameters of BAT in obese and non-obese rodents undergoing vagotomy associated with splenectomy were evaluated. For this, obesity was induced by the subcutaneous injection of monosodium glutamate (M; 4g/Kg) in the first postnatal week. Control (CTL) or nonobese rats received equimolar saline. M and CTL were weaned at 21 days, and at 60 days of life they were randomly assigned to groups according to surgery: Splenectomy (spl) – removal of the spleen; vagotomy (vag) – NV section; spl+vag (both surgeries) and false-operated (sham) – surgical stress simulation (n=6 rats/group). At 150 days of age and after 12 hours of fasting, the animals were weighed and euthanized, and the BAT of the interscapular region was removed, cleaned, washed and weighed. Part of the TAM was transferred to a fixative and submitted to histological technique and staining with hematoxylin and eosin (HE). Data were expressed as mean±SEM, using the Student's t test or two-way ANOVA with Tukey's posttest (p<0.05). M rats are smaller, with a higher BAT weight, in which there is a higher lipid content compared to CTL animals (p<0.05). In the CTL group, vag reduces body weight, BAT weight and fat deposition n in relation to sham. Spl reduces the number of nuclei and increase fat deposition in the BAT of CTL animals. On the other hand, in M-obese patients, vag does not significantly alter any parameter of the SAT, while spl increases the number of nuclei. Thus, the present study indicates that NV modulates the lipid content in the BAT of non-obese rats, without splenic interference. In obese subjects, however, the effect of NV seems to be absent, while the spleen, through an unknown route, influences the BAT of obese rats.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-05-13T12:50:22Z
dc.date.issued.fl_str_mv 2022-03-08
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv NOTTAR, Cassiane Lisbinski. A participação esplênica e vagal na deposição de lipídeos no tecido adiposo marrom de ratos obesos e não obesos. 2022. 65f. Dissertação (Mestrado em Ciências Aplicadas à Saúde) - Universidade Estadual do Oeste do Paraná, Francisco Beltrão, 2022.
dc.identifier.uri.fl_str_mv https://tede.unioeste.br/handle/tede/6036
identifier_str_mv NOTTAR, Cassiane Lisbinski. A participação esplênica e vagal na deposição de lipídeos no tecido adiposo marrom de ratos obesos e não obesos. 2022. 65f. Dissertação (Mestrado em Ciências Aplicadas à Saúde) - Universidade Estadual do Oeste do Paraná, Francisco Beltrão, 2022.
url https://tede.unioeste.br/handle/tede/6036
dc.language.iso.fl_str_mv por
language por
dc.relation.program.fl_str_mv 6290525253230630664
dc.relation.confidence.fl_str_mv 600
600
dc.relation.department.fl_str_mv 292441653440865123
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual do Oeste do Paraná
Francisco Beltrão
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Ciências Aplicadas à Saúde
dc.publisher.initials.fl_str_mv UNIOESTE
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Centro de Ciências da Saúde
publisher.none.fl_str_mv Universidade Estadual do Oeste do Paraná
Francisco Beltrão
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações do UNIOESTE
instname:Universidade Estadual do Oeste do Paraná (UNIOESTE)
instacron:UNIOESTE
instname_str Universidade Estadual do Oeste do Paraná (UNIOESTE)
instacron_str UNIOESTE
institution UNIOESTE
reponame_str Biblioteca Digital de Teses e Dissertações do UNIOESTE
collection Biblioteca Digital de Teses e Dissertações do UNIOESTE
bitstream.url.fl_str_mv http://tede.unioeste.br:8080/tede/bitstream/tede/6036/5/Cassiane_Nottar_2022.pdf
http://tede.unioeste.br:8080/tede/bitstream/tede/6036/2/license_url
http://tede.unioeste.br:8080/tede/bitstream/tede/6036/3/license_text
http://tede.unioeste.br:8080/tede/bitstream/tede/6036/4/license_rdf
http://tede.unioeste.br:8080/tede/bitstream/tede/6036/1/license.txt
bitstream.checksum.fl_str_mv 6d3ab12138e5cfab4a52004d23d79979
4afdbb8c545fd630ea7db775da747b2f
d41d8cd98f00b204e9800998ecf8427e
d41d8cd98f00b204e9800998ecf8427e
bd3efa91386c1718a7f26a329fdcb468
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
MD5
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações do UNIOESTE - Universidade Estadual do Oeste do Paraná (UNIOESTE)
repository.mail.fl_str_mv biblioteca.repositorio@unioeste.br
_version_ 1801124588199870464