A participação esplênica e vagal na deposição de lipídeos no tecido adiposo marrom de ratos obesos e não obesos
Main Author: | |
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Publication Date: | 2022 |
Format: | Master thesis |
Language: | por |
Source: | Biblioteca Digital de Teses e Dissertações do UNIOESTE |
Download full: | https://tede.unioeste.br/handle/tede/6036 |
Summary: | Brown Adipose Tissue (BAT) is specialized in thermogenesis, a process that involves the degradation of energetic substrates, especially lipids, and the formation of a mitochondrial electrochemical gradient that is dissipated by the mitochondrial uncoupling protein (UCP-1) in the form of heat. Classically, thermogenesis is stimulated by the activity of the Sympathetic Nervous System (SNS), which is reduced in obesity. Studies indicate that the parasympathetic branch, via the Vagus Nerve (NV) is able to influence thermogenesis in BAT. Obese people have vagal hyperactivity. NV also modulates the immune response, and its anti-inflammatory effects depend on the presence of the spleen. In the present study, the histological parameters of BAT in obese and non-obese rodents undergoing vagotomy associated with splenectomy were evaluated. For this, obesity was induced by the subcutaneous injection of monosodium glutamate (M; 4g/Kg) in the first postnatal week. Control (CTL) or nonobese rats received equimolar saline. M and CTL were weaned at 21 days, and at 60 days of life they were randomly assigned to groups according to surgery: Splenectomy (spl) – removal of the spleen; vagotomy (vag) – NV section; spl+vag (both surgeries) and false-operated (sham) – surgical stress simulation (n=6 rats/group). At 150 days of age and after 12 hours of fasting, the animals were weighed and euthanized, and the BAT of the interscapular region was removed, cleaned, washed and weighed. Part of the TAM was transferred to a fixative and submitted to histological technique and staining with hematoxylin and eosin (HE). Data were expressed as mean±SEM, using the Student's t test or two-way ANOVA with Tukey's posttest (p<0.05). M rats are smaller, with a higher BAT weight, in which there is a higher lipid content compared to CTL animals (p<0.05). In the CTL group, vag reduces body weight, BAT weight and fat deposition n in relation to sham. Spl reduces the number of nuclei and increase fat deposition in the BAT of CTL animals. On the other hand, in M-obese patients, vag does not significantly alter any parameter of the SAT, while spl increases the number of nuclei. Thus, the present study indicates that NV modulates the lipid content in the BAT of non-obese rats, without splenic interference. In obese subjects, however, the effect of NV seems to be absent, while the spleen, through an unknown route, influences the BAT of obese rats. |
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Grassiolli, Sabrinahttp://lattes.cnpq.br/1379417550389891Grassiolli, Sabrinahttp://lattes.cnpq.br/1379417550389891Pascotto, Claudicéia Rissohttp://lattes.cnpq.br/9474794343913304Oliveira, Julio Cezar dehttp://lattes.cnpq.br/0189760778239540http://lattes.cnpq.br/8380631497090282Nottar, Cassiane Lisbinski2022-05-13T12:50:22Z2022-03-08NOTTAR, Cassiane Lisbinski. A participação esplênica e vagal na deposição de lipídeos no tecido adiposo marrom de ratos obesos e não obesos. 2022. 65f. Dissertação (Mestrado em Ciências Aplicadas à Saúde) - Universidade Estadual do Oeste do Paraná, Francisco Beltrão, 2022.https://tede.unioeste.br/handle/tede/6036Brown Adipose Tissue (BAT) is specialized in thermogenesis, a process that involves the degradation of energetic substrates, especially lipids, and the formation of a mitochondrial electrochemical gradient that is dissipated by the mitochondrial uncoupling protein (UCP-1) in the form of heat. Classically, thermogenesis is stimulated by the activity of the Sympathetic Nervous System (SNS), which is reduced in obesity. Studies indicate that the parasympathetic branch, via the Vagus Nerve (NV) is able to influence thermogenesis in BAT. Obese people have vagal hyperactivity. NV also modulates the immune response, and its anti-inflammatory effects depend on the presence of the spleen. In the present study, the histological parameters of BAT in obese and non-obese rodents undergoing vagotomy associated with splenectomy were evaluated. For this, obesity was induced by the subcutaneous injection of monosodium glutamate (M; 4g/Kg) in the first postnatal week. Control (CTL) or nonobese rats received equimolar saline. M and CTL were weaned at 21 days, and at 60 days of life they were randomly assigned to groups according to surgery: Splenectomy (spl) – removal of the spleen; vagotomy (vag) – NV section; spl+vag (both surgeries) and false-operated (sham) – surgical stress simulation (n=6 rats/group). At 150 days of age and after 12 hours of fasting, the animals were weighed and euthanized, and the BAT of the interscapular region was removed, cleaned, washed and weighed. Part of the TAM was transferred to a fixative and submitted to histological technique and staining with hematoxylin and eosin (HE). Data were expressed as mean±SEM, using the Student's t test or two-way ANOVA with Tukey's posttest (p<0.05). M rats are smaller, with a higher BAT weight, in which there is a higher lipid content compared to CTL animals (p<0.05). In the CTL group, vag reduces body weight, BAT weight and fat deposition n in relation to sham. Spl reduces the number of nuclei and increase fat deposition in the BAT of CTL animals. On the other hand, in M-obese patients, vag does not significantly alter any parameter of the SAT, while spl increases the number of nuclei. Thus, the present study indicates that NV modulates the lipid content in the BAT of non-obese rats, without splenic interference. In obese subjects, however, the effect of NV seems to be absent, while the spleen, through an unknown route, influences the BAT of obese rats.O Tecido Adiposo Marrom (TAM) é especializado na termogênese, um processo que envolve a degradação de substratos energéticos, em especial os lipídios e a formação de um gradiente eletroquímico mitocondrial que é dissipado pela proteína desacopladora mitocondrial (UCP1) na forma de calor. Classicamente a termogênese é estimulada pela atividade do Sistema Nervoso Simpático (SNS), o qual está reduzido na obesidade. Estudos apontam que o ramo parassimpático, via Nervo Vago (NV) é capaz de influenciar a termogênese no TAM. Obesos tem hiperatividade vagal. O NV também modula a resposta imunológica, e seus efeitos anti-inflamatórios dependem da presença do baço. No presente estudo foram avaliados os parâmetros histológicos do TAM em roedores obesos e não-obesos submetidos a vagotomia associado a esplenectomia. Para tal, a obesidade foi induzida pela injeção subcutânea de glutamato monossódico (M; 4g/Kg) na primeira semana pós-natal. Ratos controles (CTL) ou não-obesos receberam solução salina equimolar. Ratos M e CTL foram desmamados aos 21 dias, e aos 60 dias de vida randomicamente distribuídos nos grupos conforme cirurgia: Esplenectomia (spl) – retirada do baço; vagotomia (vag) – secção NV; spl+vag (ambas as cirurgias) e falso-operados (sham) – simulação estresse cirúrgico (n=6 – 8 ratos/grupo). Aos 150 dias de vida e após 12h de jejum, os animais foram pesados e eutanasiados, e o TAM da região interescapular foi retirado, limpo lavado e pesado. Parte do TAM foi transferida para fixador e submetida a técnica histológica e coloração com hematoxilina e eosina (HE). Dados foram expressos como média±EPM, sendo aplicados o teste t Student´s ou ANOVA de duas vias com pós teste de Tukey (p<0,05). Ratos M são obesos, apresentando maior peso do TAM, no qual há maior teor de lipídios em relação aos animais CTL (p<0.05). No grupo CTL a vag reduziu o peso corporal, o peso do TAM e a deposição de gordura em relação aos sham. A spl reduz o número de núcleos e eleva a deposição de gordura no TAM de animais CTL. Por outro lado, nos M-obesos a vag não altera significativamente nenhum parâmetro do TAM, enquanto a spl eleva o número núcleos. Assim o presente estudo indica que NV modula o teor de lipídios no TAM de ratos não-obesos, sem interferência esplênica. Nos obesos, entretanto, o efeito do NV parece estar ausente, enquanto o baço, por via ainda não conhecida influencia o TAM de ratos obesos.Submitted by Almir Squinsani (almir.squinsani@unioeste.br) on 2022-05-13T12:50:22Z No. of bitstreams: 2 Cassiane_Nottar_2022.pdf: 1391422 bytes, checksum: 6d3ab12138e5cfab4a52004d23d79979 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2022-05-13T12:50:22Z (GMT). No. of bitstreams: 2 Cassiane_Nottar_2022.pdf: 1391422 bytes, checksum: 6d3ab12138e5cfab4a52004d23d79979 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2022-03-08application/pdfpor-5356284425524309716500Universidade Estadual do Oeste do ParanáFrancisco BeltrãoPrograma de Pós-Graduação em Ciências Aplicadas à SaúdeUNIOESTEBrasilCentro de Ciências da Saúdehttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessTermogêneseLipídiosNervo vagoBaçoObesidadeThermogenesisLipidsVagus nerveSpleenObesityCIÊNCIAS DA SAÚDEA participação esplênica e vagal na deposição de lipídeos no tecido adiposo marrom de ratos obesos e não obesosSplenic and vagal participation in lipid deposition in brown adipose tissue of obese and non-obese ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis6290525253230630664600600292441653440865123reponame:Biblioteca Digital de Teses e Dissertações do UNIOESTEinstname:Universidade Estadual do Oeste do Paraná (UNIOESTE)instacron:UNIOESTEORIGINALCassiane_Nottar_2022.pdfCassiane_Nottar_2022.pdfapplication/pdf1391422http://tede.unioeste.br:8080/tede/bitstream/tede/6036/5/Cassiane_Nottar_2022.pdf6d3ab12138e5cfab4a52004d23d79979MD55CC-LICENSElicense_urllicense_urltext/plain; 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dc.title.por.fl_str_mv |
A participação esplênica e vagal na deposição de lipídeos no tecido adiposo marrom de ratos obesos e não obesos |
dc.title.alternative.eng.fl_str_mv |
Splenic and vagal participation in lipid deposition in brown adipose tissue of obese and non-obese rats |
title |
A participação esplênica e vagal na deposição de lipídeos no tecido adiposo marrom de ratos obesos e não obesos |
spellingShingle |
A participação esplênica e vagal na deposição de lipídeos no tecido adiposo marrom de ratos obesos e não obesos Nottar, Cassiane Lisbinski Termogênese Lipídios Nervo vago Baço Obesidade Thermogenesis Lipids Vagus nerve Spleen Obesity CIÊNCIAS DA SAÚDE |
title_short |
A participação esplênica e vagal na deposição de lipídeos no tecido adiposo marrom de ratos obesos e não obesos |
title_full |
A participação esplênica e vagal na deposição de lipídeos no tecido adiposo marrom de ratos obesos e não obesos |
title_fullStr |
A participação esplênica e vagal na deposição de lipídeos no tecido adiposo marrom de ratos obesos e não obesos |
title_full_unstemmed |
A participação esplênica e vagal na deposição de lipídeos no tecido adiposo marrom de ratos obesos e não obesos |
title_sort |
A participação esplênica e vagal na deposição de lipídeos no tecido adiposo marrom de ratos obesos e não obesos |
author |
Nottar, Cassiane Lisbinski |
author_facet |
Nottar, Cassiane Lisbinski |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Grassiolli, Sabrina |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1379417550389891 |
dc.contributor.referee1.fl_str_mv |
Grassiolli, Sabrina |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/1379417550389891 |
dc.contributor.referee2.fl_str_mv |
Pascotto, Claudicéia Risso |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/9474794343913304 |
dc.contributor.referee3.fl_str_mv |
Oliveira, Julio Cezar de |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/0189760778239540 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/8380631497090282 |
dc.contributor.author.fl_str_mv |
Nottar, Cassiane Lisbinski |
contributor_str_mv |
Grassiolli, Sabrina Grassiolli, Sabrina Pascotto, Claudicéia Risso Oliveira, Julio Cezar de |
dc.subject.por.fl_str_mv |
Termogênese Lipídios Nervo vago Baço Obesidade |
topic |
Termogênese Lipídios Nervo vago Baço Obesidade Thermogenesis Lipids Vagus nerve Spleen Obesity CIÊNCIAS DA SAÚDE |
dc.subject.eng.fl_str_mv |
Thermogenesis Lipids Vagus nerve Spleen Obesity |
dc.subject.cnpq.fl_str_mv |
CIÊNCIAS DA SAÚDE |
description |
Brown Adipose Tissue (BAT) is specialized in thermogenesis, a process that involves the degradation of energetic substrates, especially lipids, and the formation of a mitochondrial electrochemical gradient that is dissipated by the mitochondrial uncoupling protein (UCP-1) in the form of heat. Classically, thermogenesis is stimulated by the activity of the Sympathetic Nervous System (SNS), which is reduced in obesity. Studies indicate that the parasympathetic branch, via the Vagus Nerve (NV) is able to influence thermogenesis in BAT. Obese people have vagal hyperactivity. NV also modulates the immune response, and its anti-inflammatory effects depend on the presence of the spleen. In the present study, the histological parameters of BAT in obese and non-obese rodents undergoing vagotomy associated with splenectomy were evaluated. For this, obesity was induced by the subcutaneous injection of monosodium glutamate (M; 4g/Kg) in the first postnatal week. Control (CTL) or nonobese rats received equimolar saline. M and CTL were weaned at 21 days, and at 60 days of life they were randomly assigned to groups according to surgery: Splenectomy (spl) – removal of the spleen; vagotomy (vag) – NV section; spl+vag (both surgeries) and false-operated (sham) – surgical stress simulation (n=6 rats/group). At 150 days of age and after 12 hours of fasting, the animals were weighed and euthanized, and the BAT of the interscapular region was removed, cleaned, washed and weighed. Part of the TAM was transferred to a fixative and submitted to histological technique and staining with hematoxylin and eosin (HE). Data were expressed as mean±SEM, using the Student's t test or two-way ANOVA with Tukey's posttest (p<0.05). M rats are smaller, with a higher BAT weight, in which there is a higher lipid content compared to CTL animals (p<0.05). In the CTL group, vag reduces body weight, BAT weight and fat deposition n in relation to sham. Spl reduces the number of nuclei and increase fat deposition in the BAT of CTL animals. On the other hand, in M-obese patients, vag does not significantly alter any parameter of the SAT, while spl increases the number of nuclei. Thus, the present study indicates that NV modulates the lipid content in the BAT of non-obese rats, without splenic interference. In obese subjects, however, the effect of NV seems to be absent, while the spleen, through an unknown route, influences the BAT of obese rats. |
publishDate |
2022 |
dc.date.accessioned.fl_str_mv |
2022-05-13T12:50:22Z |
dc.date.issued.fl_str_mv |
2022-03-08 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
NOTTAR, Cassiane Lisbinski. A participação esplênica e vagal na deposição de lipídeos no tecido adiposo marrom de ratos obesos e não obesos. 2022. 65f. Dissertação (Mestrado em Ciências Aplicadas à Saúde) - Universidade Estadual do Oeste do Paraná, Francisco Beltrão, 2022. |
dc.identifier.uri.fl_str_mv |
https://tede.unioeste.br/handle/tede/6036 |
identifier_str_mv |
NOTTAR, Cassiane Lisbinski. A participação esplênica e vagal na deposição de lipídeos no tecido adiposo marrom de ratos obesos e não obesos. 2022. 65f. Dissertação (Mestrado em Ciências Aplicadas à Saúde) - Universidade Estadual do Oeste do Paraná, Francisco Beltrão, 2022. |
url |
https://tede.unioeste.br/handle/tede/6036 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
6290525253230630664 |
dc.relation.confidence.fl_str_mv |
600 600 |
dc.relation.department.fl_str_mv |
292441653440865123 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual do Oeste do Paraná Francisco Beltrão |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciências Aplicadas à Saúde |
dc.publisher.initials.fl_str_mv |
UNIOESTE |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Centro de Ciências da Saúde |
publisher.none.fl_str_mv |
Universidade Estadual do Oeste do Paraná Francisco Beltrão |
dc.source.none.fl_str_mv |
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Biblioteca Digital de Teses e Dissertações do UNIOESTE |
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Biblioteca Digital de Teses e Dissertações do UNIOESTE - Universidade Estadual do Oeste do Paraná (UNIOESTE) |
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biblioteca.repositorio@unioeste.br |
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