Expression of beta catenin, geminin and MCM2 in sporadic keratocystic odontogenic tumor and associated with the nevoid basal cell carcinoma syndrome
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Biblioteca Digital de Teses e Dissertações do UNIOESTE |
Texto Completo: | http://tede.unioeste.br:8080/tede/handle/tede/747 |
Resumo: | Objective: The aim of this study was to investigate the beta catenin, geminin and MCM2 expression in sporadics and syndromics keratocystic odontogenic tumors (KCOTs). Material and Methods: Clinical data from 40 cases of KCOTs (30 syndromic and 10 sporadic cases) were coleted and sections from they were immunohistochemically stained and assayed for beta catenin, geminin and MCM2. Results: Sattelites cysts and cellular pleomorfism were more prevalent in syndromic cases. The beta catenin staining pattern was membranous and its reactive extension does not show statistical difference between syndromic and sporadic KCOTs, whereas the syndromic lesions showed less intense reactivity for beta catenin. The reactivity for geminin and MCM2 in both groups showed a nuclear staining pattern. In these groups, the nuclear staining occurred predominantly in the first suprabasal layer. There is no statistical difference in the geminin reactivity between the groups, whereas the means of MCM2 positive cells was higher in sporadic KCOTs than syndromic KCOTs group (p=0.011). Conclusion: Histological features shows evidences of greater aggressiveness in syndromic KCOTs, but there is not significant evidence that ensures the higher proliferative potencial of syndromic KCOT using these markers |
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Rangel, Ana Lúcia Carrinho AyrozaCPF:25268054813http://lattes.cnpq.br/1438147088984375Busato, Mauro Carlos AgnerCPF:01920157964http://lattes.cnpq.br/1189765186576000Souza, Ricardo Sampaio deCPF:16558670836http://lattes.cnpq.br/9676052176262119CPF:07152695938http://lattes.cnpq.br/9966111821073224Santos, Jéssica Luana dos2017-07-10T14:57:33Z2016-12-162016-01-29SANTOS, Jéssica Luana dos. Expression of beta catenin, geminin and MCM2 in sporadic keratocystic odontogenic tumor and associated with the nevoid basal cell carcinoma syndrome. 2016. 62 f. Dissertação (Mestrado em Odontologia) - Universidade Estadual do Oeste do Parana, Cascavel, 2016.http://tede.unioeste.br:8080/tede/handle/tede/747Objective: The aim of this study was to investigate the beta catenin, geminin and MCM2 expression in sporadics and syndromics keratocystic odontogenic tumors (KCOTs). Material and Methods: Clinical data from 40 cases of KCOTs (30 syndromic and 10 sporadic cases) were coleted and sections from they were immunohistochemically stained and assayed for beta catenin, geminin and MCM2. Results: Sattelites cysts and cellular pleomorfism were more prevalent in syndromic cases. The beta catenin staining pattern was membranous and its reactive extension does not show statistical difference between syndromic and sporadic KCOTs, whereas the syndromic lesions showed less intense reactivity for beta catenin. The reactivity for geminin and MCM2 in both groups showed a nuclear staining pattern. In these groups, the nuclear staining occurred predominantly in the first suprabasal layer. There is no statistical difference in the geminin reactivity between the groups, whereas the means of MCM2 positive cells was higher in sporadic KCOTs than syndromic KCOTs group (p=0.011). Conclusion: Histological features shows evidences of greater aggressiveness in syndromic KCOTs, but there is not significant evidence that ensures the higher proliferative potencial of syndromic KCOT using these markersObjetivo: O objetivo deste estudo foi avaliar a expressão de beta catenina, geminina e MCM2 em tumors odontogênicos queratocísticos (KCOTs) sindrômicos e esporádicos. Material e Metódos: Dados clínicos de 40 casos de KCOTs (30 casos sindrômicos e 10 esporádicos) foram coletados e cortes histológicos foram imuno-histoquimicamente corados e avaliados para beta catenina, geminina e MCM2. Resultados: Cistos satélites e pleomorfismo celular foram mais prevalentes nos casos sindrômicos. O padrão de marcação da beta catenina foi membranoso e sua reatividade avaliada em extensão não foi estatisticamente diferente entre os grupos de casos sindrômicos e esporádicos, no entanto, lesões sindrômicas apresentaram reatividade menos intensa para beta catenina do que os casos esporádicos. A reatividade da geminina e MCM2 em ambos os grupos foi nuclear. Nesses grupos, a marcação ocorreu predominantemente na camada parabasal. Não houve diferença estatística entre lesões sindrômicas e esporádicas para geminina, já a MCM2 apresentou maior média de células positivas em KCOTs esporádicos (p= 0,011). Conclusão: Características histológicas mostraram evidências de maior agressividade em KCOTs sindrômicos, mas não houve achados que confirmem o maior potencial proliferativo de KCOTs sindrômicos utilizando beta catenina, geminina e MCM2.Made available in DSpace on 2017-07-10T14:57:33Z (GMT). No. of bitstreams: 1 jessica_ santos.pdf: 1533304 bytes, checksum: 6fbddec092b8984d7f3504674acba1e9 (MD5) Previous issue date: 2016-01-29Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfengUniversidade Estadual do Oeste do ParanaPrograma de Pós-Graduação Stricto Sensu em Odontologia Nível de MestradoUNIOESTEBROdontologiaNeoplasias maxilomandibularesimuno-histoquímicaProliferação de célulasJaw neoplasmsImmunohistochemistryCell proliferationCNPQ::CIENCIAS DA SAUDE::ODONTOLOGIAExpression of beta catenin, geminin and MCM2 in sporadic keratocystic odontogenic tumor and associated with the nevoid basal cell carcinoma syndromeAvaliação da imunorreatividade da beta catenina, geminina e MCM2 em tumores queratocísticos odontogênicos esporádicos e associados à síndromedo carcinoma nevóide basocelularinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UNIOESTEinstname:Universidade Estadual do Oeste do Paraná (UNIOESTE)instacron:UNIOESTEORIGINALjessica_ santos.pdfapplication/pdf1533304http://tede.unioeste.br:8080/tede/bitstream/tede/747/1/jessica_+santos.pdf6fbddec092b8984d7f3504674acba1e9MD51tede/7472018-02-16 14:55:38.243oai:tede.unioeste.br:tede/747Biblioteca Digital de Teses e Dissertaçõeshttp://tede.unioeste.br/PUBhttp://tede.unioeste.br/oai/requestbiblioteca.repositorio@unioeste.bropendoar:2018-02-16T16:55:38Biblioteca Digital de Teses e Dissertações do UNIOESTE - Universidade Estadual do Oeste do Paraná (UNIOESTE)false |
dc.title.eng.fl_str_mv |
Expression of beta catenin, geminin and MCM2 in sporadic keratocystic odontogenic tumor and associated with the nevoid basal cell carcinoma syndrome |
dc.title.alternative.por.fl_str_mv |
Avaliação da imunorreatividade da beta catenina, geminina e MCM2 em tumores queratocísticos odontogênicos esporádicos e associados à síndromedo carcinoma nevóide basocelular |
title |
Expression of beta catenin, geminin and MCM2 in sporadic keratocystic odontogenic tumor and associated with the nevoid basal cell carcinoma syndrome |
spellingShingle |
Expression of beta catenin, geminin and MCM2 in sporadic keratocystic odontogenic tumor and associated with the nevoid basal cell carcinoma syndrome Santos, Jéssica Luana dos Neoplasias maxilomandibulares imuno-histoquímica Proliferação de células Jaw neoplasms Immunohistochemistry Cell proliferation CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
title_short |
Expression of beta catenin, geminin and MCM2 in sporadic keratocystic odontogenic tumor and associated with the nevoid basal cell carcinoma syndrome |
title_full |
Expression of beta catenin, geminin and MCM2 in sporadic keratocystic odontogenic tumor and associated with the nevoid basal cell carcinoma syndrome |
title_fullStr |
Expression of beta catenin, geminin and MCM2 in sporadic keratocystic odontogenic tumor and associated with the nevoid basal cell carcinoma syndrome |
title_full_unstemmed |
Expression of beta catenin, geminin and MCM2 in sporadic keratocystic odontogenic tumor and associated with the nevoid basal cell carcinoma syndrome |
title_sort |
Expression of beta catenin, geminin and MCM2 in sporadic keratocystic odontogenic tumor and associated with the nevoid basal cell carcinoma syndrome |
author |
Santos, Jéssica Luana dos |
author_facet |
Santos, Jéssica Luana dos |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Rangel, Ana Lúcia Carrinho Ayroza |
dc.contributor.advisor1ID.fl_str_mv |
CPF:25268054813 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1438147088984375 |
dc.contributor.referee1.fl_str_mv |
Busato, Mauro Carlos Agner |
dc.contributor.referee1ID.fl_str_mv |
CPF:01920157964 |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/1189765186576000 |
dc.contributor.referee2.fl_str_mv |
Souza, Ricardo Sampaio de |
dc.contributor.referee2ID.fl_str_mv |
CPF:16558670836 |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/9676052176262119 |
dc.contributor.authorID.fl_str_mv |
CPF:07152695938 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/9966111821073224 |
dc.contributor.author.fl_str_mv |
Santos, Jéssica Luana dos |
contributor_str_mv |
Rangel, Ana Lúcia Carrinho Ayroza Busato, Mauro Carlos Agner Souza, Ricardo Sampaio de |
dc.subject.por.fl_str_mv |
Neoplasias maxilomandibulares imuno-histoquímica Proliferação de células |
topic |
Neoplasias maxilomandibulares imuno-histoquímica Proliferação de células Jaw neoplasms Immunohistochemistry Cell proliferation CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
dc.subject.eng.fl_str_mv |
Jaw neoplasms Immunohistochemistry Cell proliferation |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
description |
Objective: The aim of this study was to investigate the beta catenin, geminin and MCM2 expression in sporadics and syndromics keratocystic odontogenic tumors (KCOTs). Material and Methods: Clinical data from 40 cases of KCOTs (30 syndromic and 10 sporadic cases) were coleted and sections from they were immunohistochemically stained and assayed for beta catenin, geminin and MCM2. Results: Sattelites cysts and cellular pleomorfism were more prevalent in syndromic cases. The beta catenin staining pattern was membranous and its reactive extension does not show statistical difference between syndromic and sporadic KCOTs, whereas the syndromic lesions showed less intense reactivity for beta catenin. The reactivity for geminin and MCM2 in both groups showed a nuclear staining pattern. In these groups, the nuclear staining occurred predominantly in the first suprabasal layer. There is no statistical difference in the geminin reactivity between the groups, whereas the means of MCM2 positive cells was higher in sporadic KCOTs than syndromic KCOTs group (p=0.011). Conclusion: Histological features shows evidences of greater aggressiveness in syndromic KCOTs, but there is not significant evidence that ensures the higher proliferative potencial of syndromic KCOT using these markers |
publishDate |
2016 |
dc.date.available.fl_str_mv |
2016-12-16 |
dc.date.issued.fl_str_mv |
2016-01-29 |
dc.date.accessioned.fl_str_mv |
2017-07-10T14:57:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SANTOS, Jéssica Luana dos. Expression of beta catenin, geminin and MCM2 in sporadic keratocystic odontogenic tumor and associated with the nevoid basal cell carcinoma syndrome. 2016. 62 f. Dissertação (Mestrado em Odontologia) - Universidade Estadual do Oeste do Parana, Cascavel, 2016. |
dc.identifier.uri.fl_str_mv |
http://tede.unioeste.br:8080/tede/handle/tede/747 |
identifier_str_mv |
SANTOS, Jéssica Luana dos. Expression of beta catenin, geminin and MCM2 in sporadic keratocystic odontogenic tumor and associated with the nevoid basal cell carcinoma syndrome. 2016. 62 f. Dissertação (Mestrado em Odontologia) - Universidade Estadual do Oeste do Parana, Cascavel, 2016. |
url |
http://tede.unioeste.br:8080/tede/handle/tede/747 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual do Oeste do Parana |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação Stricto Sensu em Odontologia Nível de Mestrado |
dc.publisher.initials.fl_str_mv |
UNIOESTE |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Odontologia |
publisher.none.fl_str_mv |
Universidade Estadual do Oeste do Parana |
dc.source.none.fl_str_mv |
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UNIOESTE |
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UNIOESTE |
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Biblioteca Digital de Teses e Dissertações do UNIOESTE |
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Biblioteca Digital de Teses e Dissertações do UNIOESTE |
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Biblioteca Digital de Teses e Dissertações do UNIOESTE - Universidade Estadual do Oeste do Paraná (UNIOESTE) |
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biblioteca.repositorio@unioeste.br |
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