Expression of beta catenin, geminin and MCM2 in sporadic keratocystic odontogenic tumor and associated with the nevoid basal cell carcinoma syndrome

Detalhes bibliográficos
Autor(a) principal: Santos, Jéssica Luana dos
Data de Publicação: 2016
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Biblioteca Digital de Teses e Dissertações do UNIOESTE
Texto Completo: http://tede.unioeste.br:8080/tede/handle/tede/747
Resumo: Objective: The aim of this study was to investigate the beta catenin, geminin and MCM2 expression in sporadics and syndromics keratocystic odontogenic tumors (KCOTs). Material and Methods: Clinical data from 40 cases of KCOTs (30 syndromic and 10 sporadic cases) were coleted and sections from they were immunohistochemically stained and assayed for beta catenin, geminin and MCM2. Results: Sattelites cysts and cellular pleomorfism were more prevalent in syndromic cases. The beta catenin staining pattern was membranous and its reactive extension does not show statistical difference between syndromic and sporadic KCOTs, whereas the syndromic lesions showed less intense reactivity for beta catenin. The reactivity for geminin and MCM2 in both groups showed a nuclear staining pattern. In these groups, the nuclear staining occurred predominantly in the first suprabasal layer. There is no statistical difference in the geminin reactivity between the groups, whereas the means of MCM2 positive cells was higher in sporadic KCOTs than syndromic KCOTs group (p=0.011). Conclusion: Histological features shows evidences of greater aggressiveness in syndromic KCOTs, but there is not significant evidence that ensures the higher proliferative potencial of syndromic KCOT using these markers
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spelling Rangel, Ana Lúcia Carrinho AyrozaCPF:25268054813http://lattes.cnpq.br/1438147088984375Busato, Mauro Carlos AgnerCPF:01920157964http://lattes.cnpq.br/1189765186576000Souza, Ricardo Sampaio deCPF:16558670836http://lattes.cnpq.br/9676052176262119CPF:07152695938http://lattes.cnpq.br/9966111821073224Santos, Jéssica Luana dos2017-07-10T14:57:33Z2016-12-162016-01-29SANTOS, Jéssica Luana dos. Expression of beta catenin, geminin and MCM2 in sporadic keratocystic odontogenic tumor and associated with the nevoid basal cell carcinoma syndrome. 2016. 62 f. Dissertação (Mestrado em Odontologia) - Universidade Estadual do Oeste do Parana, Cascavel, 2016.http://tede.unioeste.br:8080/tede/handle/tede/747Objective: The aim of this study was to investigate the beta catenin, geminin and MCM2 expression in sporadics and syndromics keratocystic odontogenic tumors (KCOTs). Material and Methods: Clinical data from 40 cases of KCOTs (30 syndromic and 10 sporadic cases) were coleted and sections from they were immunohistochemically stained and assayed for beta catenin, geminin and MCM2. Results: Sattelites cysts and cellular pleomorfism were more prevalent in syndromic cases. The beta catenin staining pattern was membranous and its reactive extension does not show statistical difference between syndromic and sporadic KCOTs, whereas the syndromic lesions showed less intense reactivity for beta catenin. The reactivity for geminin and MCM2 in both groups showed a nuclear staining pattern. In these groups, the nuclear staining occurred predominantly in the first suprabasal layer. There is no statistical difference in the geminin reactivity between the groups, whereas the means of MCM2 positive cells was higher in sporadic KCOTs than syndromic KCOTs group (p=0.011). Conclusion: Histological features shows evidences of greater aggressiveness in syndromic KCOTs, but there is not significant evidence that ensures the higher proliferative potencial of syndromic KCOT using these markersObjetivo: O objetivo deste estudo foi avaliar a expressão de beta catenina, geminina e MCM2 em tumors odontogênicos queratocísticos (KCOTs) sindrômicos e esporádicos. Material e Metódos: Dados clínicos de 40 casos de KCOTs (30 casos sindrômicos e 10 esporádicos) foram coletados e cortes histológicos foram imuno-histoquimicamente corados e avaliados para beta catenina, geminina e MCM2. Resultados: Cistos satélites e pleomorfismo celular foram mais prevalentes nos casos sindrômicos. O padrão de marcação da beta catenina foi membranoso e sua reatividade avaliada em extensão não foi estatisticamente diferente entre os grupos de casos sindrômicos e esporádicos, no entanto, lesões sindrômicas apresentaram reatividade menos intensa para beta catenina do que os casos esporádicos. A reatividade da geminina e MCM2 em ambos os grupos foi nuclear. Nesses grupos, a marcação ocorreu predominantemente na camada parabasal. Não houve diferença estatística entre lesões sindrômicas e esporádicas para geminina, já a MCM2 apresentou maior média de células positivas em KCOTs esporádicos (p= 0,011). Conclusão: Características histológicas mostraram evidências de maior agressividade em KCOTs sindrômicos, mas não houve achados que confirmem o maior potencial proliferativo de KCOTs sindrômicos utilizando beta catenina, geminina e MCM2.Made available in DSpace on 2017-07-10T14:57:33Z (GMT). No. of bitstreams: 1 jessica_ santos.pdf: 1533304 bytes, checksum: 6fbddec092b8984d7f3504674acba1e9 (MD5) Previous issue date: 2016-01-29Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfengUniversidade Estadual do Oeste do ParanaPrograma de Pós-Graduação Stricto Sensu em Odontologia Nível de MestradoUNIOESTEBROdontologiaNeoplasias maxilomandibularesimuno-histoquímicaProliferação de célulasJaw neoplasmsImmunohistochemistryCell proliferationCNPQ::CIENCIAS DA SAUDE::ODONTOLOGIAExpression of beta catenin, geminin and MCM2 in sporadic keratocystic odontogenic tumor and associated with the nevoid basal cell carcinoma syndromeAvaliação da imunorreatividade da beta catenina, geminina e MCM2 em tumores queratocísticos odontogênicos esporádicos e associados à síndromedo carcinoma nevóide basocelularinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UNIOESTEinstname:Universidade Estadual do Oeste do Paraná (UNIOESTE)instacron:UNIOESTEORIGINALjessica_ santos.pdfapplication/pdf1533304http://tede.unioeste.br:8080/tede/bitstream/tede/747/1/jessica_+santos.pdf6fbddec092b8984d7f3504674acba1e9MD51tede/7472018-02-16 14:55:38.243oai:tede.unioeste.br:tede/747Biblioteca Digital de Teses e Dissertaçõeshttp://tede.unioeste.br/PUBhttp://tede.unioeste.br/oai/requestbiblioteca.repositorio@unioeste.bropendoar:2018-02-16T16:55:38Biblioteca Digital de Teses e Dissertações do UNIOESTE - Universidade Estadual do Oeste do Paraná (UNIOESTE)false
dc.title.eng.fl_str_mv Expression of beta catenin, geminin and MCM2 in sporadic keratocystic odontogenic tumor and associated with the nevoid basal cell carcinoma syndrome
dc.title.alternative.por.fl_str_mv Avaliação da imunorreatividade da beta catenina, geminina e MCM2 em tumores queratocísticos odontogênicos esporádicos e associados à síndromedo carcinoma nevóide basocelular
title Expression of beta catenin, geminin and MCM2 in sporadic keratocystic odontogenic tumor and associated with the nevoid basal cell carcinoma syndrome
spellingShingle Expression of beta catenin, geminin and MCM2 in sporadic keratocystic odontogenic tumor and associated with the nevoid basal cell carcinoma syndrome
Santos, Jéssica Luana dos
Neoplasias maxilomandibulares
imuno-histoquímica
Proliferação de células
Jaw neoplasms
Immunohistochemistry
Cell proliferation
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
title_short Expression of beta catenin, geminin and MCM2 in sporadic keratocystic odontogenic tumor and associated with the nevoid basal cell carcinoma syndrome
title_full Expression of beta catenin, geminin and MCM2 in sporadic keratocystic odontogenic tumor and associated with the nevoid basal cell carcinoma syndrome
title_fullStr Expression of beta catenin, geminin and MCM2 in sporadic keratocystic odontogenic tumor and associated with the nevoid basal cell carcinoma syndrome
title_full_unstemmed Expression of beta catenin, geminin and MCM2 in sporadic keratocystic odontogenic tumor and associated with the nevoid basal cell carcinoma syndrome
title_sort Expression of beta catenin, geminin and MCM2 in sporadic keratocystic odontogenic tumor and associated with the nevoid basal cell carcinoma syndrome
author Santos, Jéssica Luana dos
author_facet Santos, Jéssica Luana dos
author_role author
dc.contributor.advisor1.fl_str_mv Rangel, Ana Lúcia Carrinho Ayroza
dc.contributor.advisor1ID.fl_str_mv CPF:25268054813
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/1438147088984375
dc.contributor.referee1.fl_str_mv Busato, Mauro Carlos Agner
dc.contributor.referee1ID.fl_str_mv CPF:01920157964
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/1189765186576000
dc.contributor.referee2.fl_str_mv Souza, Ricardo Sampaio de
dc.contributor.referee2ID.fl_str_mv CPF:16558670836
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/9676052176262119
dc.contributor.authorID.fl_str_mv CPF:07152695938
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/9966111821073224
dc.contributor.author.fl_str_mv Santos, Jéssica Luana dos
contributor_str_mv Rangel, Ana Lúcia Carrinho Ayroza
Busato, Mauro Carlos Agner
Souza, Ricardo Sampaio de
dc.subject.por.fl_str_mv Neoplasias maxilomandibulares
imuno-histoquímica
Proliferação de células
topic Neoplasias maxilomandibulares
imuno-histoquímica
Proliferação de células
Jaw neoplasms
Immunohistochemistry
Cell proliferation
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
dc.subject.eng.fl_str_mv Jaw neoplasms
Immunohistochemistry
Cell proliferation
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
description Objective: The aim of this study was to investigate the beta catenin, geminin and MCM2 expression in sporadics and syndromics keratocystic odontogenic tumors (KCOTs). Material and Methods: Clinical data from 40 cases of KCOTs (30 syndromic and 10 sporadic cases) were coleted and sections from they were immunohistochemically stained and assayed for beta catenin, geminin and MCM2. Results: Sattelites cysts and cellular pleomorfism were more prevalent in syndromic cases. The beta catenin staining pattern was membranous and its reactive extension does not show statistical difference between syndromic and sporadic KCOTs, whereas the syndromic lesions showed less intense reactivity for beta catenin. The reactivity for geminin and MCM2 in both groups showed a nuclear staining pattern. In these groups, the nuclear staining occurred predominantly in the first suprabasal layer. There is no statistical difference in the geminin reactivity between the groups, whereas the means of MCM2 positive cells was higher in sporadic KCOTs than syndromic KCOTs group (p=0.011). Conclusion: Histological features shows evidences of greater aggressiveness in syndromic KCOTs, but there is not significant evidence that ensures the higher proliferative potencial of syndromic KCOT using these markers
publishDate 2016
dc.date.available.fl_str_mv 2016-12-16
dc.date.issued.fl_str_mv 2016-01-29
dc.date.accessioned.fl_str_mv 2017-07-10T14:57:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, Jéssica Luana dos. Expression of beta catenin, geminin and MCM2 in sporadic keratocystic odontogenic tumor and associated with the nevoid basal cell carcinoma syndrome. 2016. 62 f. Dissertação (Mestrado em Odontologia) - Universidade Estadual do Oeste do Parana, Cascavel, 2016.
dc.identifier.uri.fl_str_mv http://tede.unioeste.br:8080/tede/handle/tede/747
identifier_str_mv SANTOS, Jéssica Luana dos. Expression of beta catenin, geminin and MCM2 in sporadic keratocystic odontogenic tumor and associated with the nevoid basal cell carcinoma syndrome. 2016. 62 f. Dissertação (Mestrado em Odontologia) - Universidade Estadual do Oeste do Parana, Cascavel, 2016.
url http://tede.unioeste.br:8080/tede/handle/tede/747
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual do Oeste do Parana
dc.publisher.program.fl_str_mv Programa de Pós-Graduação Stricto Sensu em Odontologia Nível de Mestrado
dc.publisher.initials.fl_str_mv UNIOESTE
dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Odontologia
publisher.none.fl_str_mv Universidade Estadual do Oeste do Parana
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações do UNIOESTE
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reponame_str Biblioteca Digital de Teses e Dissertações do UNIOESTE
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