Estudo da cinética da hidrólise enzimática do óleo de soja: experimental e modelagem

Detalhes bibliográficos
Autor(a) principal: Gurtat, Meline
Data de Publicação: 2018
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações do UNIOESTE
Texto Completo: https://tede.unioeste.br/handle/tede/6828
Resumo: The objective of this work was to evaluate the kinetic hydrolysis of soybean oil by action of the Lipozyme TL IM enzyme, especially the effect of the operational conditions such as water/oil molar ratio and temperature. In addition, to evaluate the capability of mathematical models to describe kinetic data including all compounds of the reaction (acylglycerols, glycerol, free fatty acids and water). Kinetic experiments, in batch reactor, were performed at different water/oil molar ratios (9:1, 20:1, 46:1 and 60:1) and temperatures (40, 52 and 64°C) under constant enzyme dosage (1% in relation to the oil mass) and stirring speed (250 rpm). The reaction yield was determined based on the free fatty acids concentration. Mathematical models based on the simplified Bi Bi Ping-Pong kinetic mechanism were used to describe kinetic data. The models were applied considering inhibition and non-inhibition by products of the reaction (without competitive inhibition; with inhibition by the free fatty acid; with inhibition by glycerol, with inhibition of both products). In the 46:1 and 60:1 water/oil molar ratios at temperatures of 52 and 64°C was achieved the higher reaction yield (77%). From the criterion of information Akaike and coefficient of determination values, it was verified that the mathematical model that consider non-inhibition by reaction products described better kinetic experimental data in all conditions. In addition, this model provided physically consistent results of the concentration profiles of the reaction compounds (triacylglycerols, diacylglycerols, monoacylglycerols, glycerol, free fatty acid and water).
id UNIOESTE-1_e3d302f3911dd10db81ca3b8e838c870
oai_identifier_str oai:tede.unioeste.br:tede/6828
network_acronym_str UNIOESTE-1
network_name_str Biblioteca Digital de Teses e Dissertações do UNIOESTE
repository_id_str
spelling Borba, Carlos Eduardohttp://lattes.cnpq.br/0750048720229101Silva, Camila dahttp://lattes.cnpq.br/0151285357488920Trigueros, Daniela Estelita Goeshttp://lattes.cnpq.br/5198880627063850Tormen, Lucianohttp://lattes.cnpq.br/0722056210523421Borba, Carlos Eduardohttp://lattes.cnpq.br/0750048720229101http://lattes.cnpq.br/4581485660515978Gurtat, Meline2023-09-23T00:52:02Z2018-03-22GURTAT, Meline. Estudo da cinética da hidrólise enzimática do óleo de soja: experimental e modelagem. 2018. 70 f. Dissertação (Mestrado em Engenharia Química) - Universidade Estadual do Oeste do Paraná, Toledo, 2018.https://tede.unioeste.br/handle/tede/6828The objective of this work was to evaluate the kinetic hydrolysis of soybean oil by action of the Lipozyme TL IM enzyme, especially the effect of the operational conditions such as water/oil molar ratio and temperature. In addition, to evaluate the capability of mathematical models to describe kinetic data including all compounds of the reaction (acylglycerols, glycerol, free fatty acids and water). Kinetic experiments, in batch reactor, were performed at different water/oil molar ratios (9:1, 20:1, 46:1 and 60:1) and temperatures (40, 52 and 64°C) under constant enzyme dosage (1% in relation to the oil mass) and stirring speed (250 rpm). The reaction yield was determined based on the free fatty acids concentration. Mathematical models based on the simplified Bi Bi Ping-Pong kinetic mechanism were used to describe kinetic data. The models were applied considering inhibition and non-inhibition by products of the reaction (without competitive inhibition; with inhibition by the free fatty acid; with inhibition by glycerol, with inhibition of both products). In the 46:1 and 60:1 water/oil molar ratios at temperatures of 52 and 64°C was achieved the higher reaction yield (77%). From the criterion of information Akaike and coefficient of determination values, it was verified that the mathematical model that consider non-inhibition by reaction products described better kinetic experimental data in all conditions. In addition, this model provided physically consistent results of the concentration profiles of the reaction compounds (triacylglycerols, diacylglycerols, monoacylglycerols, glycerol, free fatty acid and water).O objetivo do presente trabalho foi avaliar a cinética da hidrólise enzimática do óleo de soja pela ação da enzima Lipozyme TL IM diante da variação de condições experimentais razão molar água/óleo e temperatura, bem como determinar por meio de modelagem matemática, os perfis de concentrações de acilgliceróis (TAG, DAG e MAG), glicerol, e ácidos graxos livres e água. Para tanto, foram realizados experimentos cinéticos em reator batelada tipo tanque agitado. Os experimentos foram conduzidos com diferentes razões molares água/óleo (9:1, 20:1, 46:1 e 60:1) e diferentes temperaturas (40, 52 e 64°C), em condições constantes de dosagem de enzima (1% em relação a massa de óleo) e velocidade de agitação (250 rpm). O rendimento das reações foi quantificado em termos da concentração de ácidos graxos livres. Para descrever a cinética de hidrólise enzimática do óleo de soja, foi empregado o mecanismo de Pingue-Pongue Bi Bi Simplificado considerando quatro hipóteses: sem inibição competitiva; com inibição pelo ácido graxo livre; com inibição pelo glicerol, e, com inibição de ambos os produtos. Os melhores rendimentos foram obtidos utilizando a razão molar água/óleo 46:1 e 60:1, nas temperaturas de 52 e 64°C (ambas com 77%), indicando a influência destas variáveis no processo de hidrólise do óleo pela ação da enzima Lipozyme TL IM. A partir dos valores do critério corrigido de informação Akaike e do coeficiente de determinação foi verificado que o Modelo 1 – Mecanismo de Pingue-Pongue Bi Bi Simplificado sem inibição competitiva descreveu bem curvas cinéticas experimentais e, forneceu resultados fisicamente consistentes dos perfis de concentrações dos demais componentes da reação (triacilgliceróis, diacilgliceróis, monoacilgliceróis, glicerol, ácido graxo livre e água).Submitted by Marilene Donadel (marilene.donadel@unioeste.br) on 2023-09-23T00:52:02Z No. of bitstreams: 1 Meline_Gurtat_2018.pdf: 2640446 bytes, checksum: 2a22e692c5fed7976375c71ec56e0830 (MD5)Made available in DSpace on 2023-09-23T00:52:02Z (GMT). No. of bitstreams: 1 Meline_Gurtat_2018.pdf: 2640446 bytes, checksum: 2a22e692c5fed7976375c71ec56e0830 (MD5) Previous issue date: 2018-03-22Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfpor-2624803687637593200500Universidade Estadual do Oeste do ParanáToledoPrograma de Pós-Graduação em Engenharia QuímicaUNIOESTEBrasilCentro de Engenharias e Ciências ExatasHidrólise enzimáticaÓleo de sojaLipozyme TL IMModelagem matemáticaEnzymatic hydrolysisSoybean oilMathematical modeling.ENGENHARIA QUIMICA::PROCESSOS INDUSTRIAIS DE ENGENHARIA QUIMICAEstudo da cinética da hidrólise enzimática do óleo de soja: experimental e modelagemStudy of the kinetics of the enzymatic hydrolysis of soybean oil: experimental and modelinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis1582274381427649589600600600600-773440212408214692288981387697583185912075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UNIOESTEinstname:Universidade Estadual do Oeste do Paraná (UNIOESTE)instacron:UNIOESTEORIGINALMeline_Gurtat_2018.pdfMeline_Gurtat_2018.pdfapplication/pdf2640446http://tede.unioeste.br:8080/tede/bitstream/tede/6828/2/Meline_Gurtat_2018.pdf2a22e692c5fed7976375c71ec56e0830MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://tede.unioeste.br:8080/tede/bitstream/tede/6828/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/68282023-10-02 17:08:05.137oai:tede.unioeste.br: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede.unioeste.br/PUBhttp://tede.unioeste.br/oai/requestbiblioteca.repositorio@unioeste.bropendoar:2023-10-02T20:08:05Biblioteca Digital de Teses e Dissertações do UNIOESTE - Universidade Estadual do Oeste do Paraná (UNIOESTE)false
dc.title.por.fl_str_mv Estudo da cinética da hidrólise enzimática do óleo de soja: experimental e modelagem
dc.title.alternative.eng.fl_str_mv Study of the kinetics of the enzymatic hydrolysis of soybean oil: experimental and modeling
title Estudo da cinética da hidrólise enzimática do óleo de soja: experimental e modelagem
spellingShingle Estudo da cinética da hidrólise enzimática do óleo de soja: experimental e modelagem
Gurtat, Meline
Hidrólise enzimática
Óleo de soja
Lipozyme TL IM
Modelagem matemática
Enzymatic hydrolysis
Soybean oil
Mathematical modeling.
ENGENHARIA QUIMICA::PROCESSOS INDUSTRIAIS DE ENGENHARIA QUIMICA
title_short Estudo da cinética da hidrólise enzimática do óleo de soja: experimental e modelagem
title_full Estudo da cinética da hidrólise enzimática do óleo de soja: experimental e modelagem
title_fullStr Estudo da cinética da hidrólise enzimática do óleo de soja: experimental e modelagem
title_full_unstemmed Estudo da cinética da hidrólise enzimática do óleo de soja: experimental e modelagem
title_sort Estudo da cinética da hidrólise enzimática do óleo de soja: experimental e modelagem
author Gurtat, Meline
author_facet Gurtat, Meline
author_role author
dc.contributor.advisor1.fl_str_mv Borba, Carlos Eduardo
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/0750048720229101
dc.contributor.advisor-co1.fl_str_mv Silva, Camila da
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/0151285357488920
dc.contributor.referee1.fl_str_mv Trigueros, Daniela Estelita Goes
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/5198880627063850
dc.contributor.referee2.fl_str_mv Tormen, Luciano
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/0722056210523421
dc.contributor.referee3.fl_str_mv Borba, Carlos Eduardo
dc.contributor.referee3Lattes.fl_str_mv http://lattes.cnpq.br/0750048720229101
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/4581485660515978
dc.contributor.author.fl_str_mv Gurtat, Meline
contributor_str_mv Borba, Carlos Eduardo
Silva, Camila da
Trigueros, Daniela Estelita Goes
Tormen, Luciano
Borba, Carlos Eduardo
dc.subject.por.fl_str_mv Hidrólise enzimática
Óleo de soja
Lipozyme TL IM
Modelagem matemática
topic Hidrólise enzimática
Óleo de soja
Lipozyme TL IM
Modelagem matemática
Enzymatic hydrolysis
Soybean oil
Mathematical modeling.
ENGENHARIA QUIMICA::PROCESSOS INDUSTRIAIS DE ENGENHARIA QUIMICA
dc.subject.eng.fl_str_mv Enzymatic hydrolysis
Soybean oil
Mathematical modeling.
dc.subject.cnpq.fl_str_mv ENGENHARIA QUIMICA::PROCESSOS INDUSTRIAIS DE ENGENHARIA QUIMICA
description The objective of this work was to evaluate the kinetic hydrolysis of soybean oil by action of the Lipozyme TL IM enzyme, especially the effect of the operational conditions such as water/oil molar ratio and temperature. In addition, to evaluate the capability of mathematical models to describe kinetic data including all compounds of the reaction (acylglycerols, glycerol, free fatty acids and water). Kinetic experiments, in batch reactor, were performed at different water/oil molar ratios (9:1, 20:1, 46:1 and 60:1) and temperatures (40, 52 and 64°C) under constant enzyme dosage (1% in relation to the oil mass) and stirring speed (250 rpm). The reaction yield was determined based on the free fatty acids concentration. Mathematical models based on the simplified Bi Bi Ping-Pong kinetic mechanism were used to describe kinetic data. The models were applied considering inhibition and non-inhibition by products of the reaction (without competitive inhibition; with inhibition by the free fatty acid; with inhibition by glycerol, with inhibition of both products). In the 46:1 and 60:1 water/oil molar ratios at temperatures of 52 and 64°C was achieved the higher reaction yield (77%). From the criterion of information Akaike and coefficient of determination values, it was verified that the mathematical model that consider non-inhibition by reaction products described better kinetic experimental data in all conditions. In addition, this model provided physically consistent results of the concentration profiles of the reaction compounds (triacylglycerols, diacylglycerols, monoacylglycerols, glycerol, free fatty acid and water).
publishDate 2018
dc.date.issued.fl_str_mv 2018-03-22
dc.date.accessioned.fl_str_mv 2023-09-23T00:52:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv GURTAT, Meline. Estudo da cinética da hidrólise enzimática do óleo de soja: experimental e modelagem. 2018. 70 f. Dissertação (Mestrado em Engenharia Química) - Universidade Estadual do Oeste do Paraná, Toledo, 2018.
dc.identifier.uri.fl_str_mv https://tede.unioeste.br/handle/tede/6828
identifier_str_mv GURTAT, Meline. Estudo da cinética da hidrólise enzimática do óleo de soja: experimental e modelagem. 2018. 70 f. Dissertação (Mestrado em Engenharia Química) - Universidade Estadual do Oeste do Paraná, Toledo, 2018.
url https://tede.unioeste.br/handle/tede/6828
dc.language.iso.fl_str_mv por
language por
dc.relation.program.fl_str_mv 1582274381427649589
dc.relation.confidence.fl_str_mv 600
600
600
600
dc.relation.department.fl_str_mv -7734402124082146922
dc.relation.cnpq.fl_str_mv 8898138769758318591
dc.relation.sponsorship.fl_str_mv 2075167498588264571
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual do Oeste do Paraná
Toledo
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Engenharia Química
dc.publisher.initials.fl_str_mv UNIOESTE
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Centro de Engenharias e Ciências Exatas
publisher.none.fl_str_mv Universidade Estadual do Oeste do Paraná
Toledo
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações do UNIOESTE
instname:Universidade Estadual do Oeste do Paraná (UNIOESTE)
instacron:UNIOESTE
instname_str Universidade Estadual do Oeste do Paraná (UNIOESTE)
instacron_str UNIOESTE
institution UNIOESTE
reponame_str Biblioteca Digital de Teses e Dissertações do UNIOESTE
collection Biblioteca Digital de Teses e Dissertações do UNIOESTE
bitstream.url.fl_str_mv http://tede.unioeste.br:8080/tede/bitstream/tede/6828/2/Meline_Gurtat_2018.pdf
http://tede.unioeste.br:8080/tede/bitstream/tede/6828/1/license.txt
bitstream.checksum.fl_str_mv 2a22e692c5fed7976375c71ec56e0830
bd3efa91386c1718a7f26a329fdcb468
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações do UNIOESTE - Universidade Estadual do Oeste do Paraná (UNIOESTE)
repository.mail.fl_str_mv biblioteca.repositorio@unioeste.br
_version_ 1811723476476624896

Registros relacionados