EVALUATION OF MODIFIED AGGLUTINATION TEST AND ENZYME-LINKED IMMUNOSORBENT ASSAY IN DETECTION OF Toxoplasma gondii ANTIBODY IN HUMAN SERA
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Arquivos de Ciências da Saúde da UNIPAR (Online) |
Texto Completo: | https://revistas.unipar.br/index.php/saude/article/view/4561 |
Resumo: | Toxoplasmosis is a zoonosis of high prevalence around the world, leading to serious congenital alterations and in immune compromised individuals. Its diagnosis is based in the detection of serum antibodies. This work aimed to compare serum antibody to Toxoplasma gondii detection by ELISA for IgG antibodies, and the MAT with the results of IFAT. From 73 samples examined by IgG-IFAT, 53 (72.6%) were positive, IgG-ELISA 55 (75.4%) and MAT, 58 (79.4%). No significative differences among results of tests are found. MAT shows high sensibility (100%) and lower specificity (75%), there were not false-negative results, but showed false-positives (25%). ELISA showed sensibility of 98.1% and specificity of 85,0%, false-negative rate of 1.9% and false-positive rate of 15.0%. MAT performance, when compared with IFAT, suggests the utilization of this method in diagnostic and epidemiological preliminary tests, besides ELISA has been used as a confirmatory test, for its specificity. |
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Arquivos de Ciências da Saúde da UNIPAR (Online) |
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EVALUATION OF MODIFIED AGGLUTINATION TEST AND ENZYME-LINKED IMMUNOSORBENT ASSAY IN DETECTION OF Toxoplasma gondii ANTIBODY IN HUMAN SERAToxoplasmosis is a zoonosis of high prevalence around the world, leading to serious congenital alterations and in immune compromised individuals. Its diagnosis is based in the detection of serum antibodies. This work aimed to compare serum antibody to Toxoplasma gondii detection by ELISA for IgG antibodies, and the MAT with the results of IFAT. From 73 samples examined by IgG-IFAT, 53 (72.6%) were positive, IgG-ELISA 55 (75.4%) and MAT, 58 (79.4%). No significative differences among results of tests are found. MAT shows high sensibility (100%) and lower specificity (75%), there were not false-negative results, but showed false-positives (25%). ELISA showed sensibility of 98.1% and specificity of 85,0%, false-negative rate of 1.9% and false-positive rate of 15.0%. MAT performance, when compared with IFAT, suggests the utilization of this method in diagnostic and epidemiological preliminary tests, besides ELISA has been used as a confirmatory test, for its specificity.UNIPAR2014-05-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistas.unipar.br/index.php/saude/article/view/4561Arquivos de Ciências da Saúde da UNIPAR; v. 16 n. 1 (2012)1982-114Xreponame:Arquivos de Ciências da Saúde da UNIPAR (Online)instname:Universidade Paranaense (UNIPAR)instacron:UNIPARporhttps://revistas.unipar.br/index.php/saude/article/view/4561/2749Silva, Dayse Vaniele daMarques, Joil MoreiraCorrea, Nelton Anderson BespalezVelasquez, Leonardo GarciaSilva, Rodrigo Costa daLangoni, HelioSöhsten, Adriana Lebram vonSilva, Aristeu Vieira dainfo:eu-repo/semantics/openAccess2021-03-26T09:10:53Zoai:ojs2.revistas.unipar.br:article/4561Revistahttp://revistas.unipar.br/index.php/saudehttp://revistas.unipar.br/saude/oai||cedic@unipar.br|| arqsaude@unipar.br1982-114X1415-076Xopendoar:2021-03-26T09:10:53Arquivos de Ciências da Saúde da UNIPAR (Online) - Universidade Paranaense (UNIPAR)false |
dc.title.none.fl_str_mv |
EVALUATION OF MODIFIED AGGLUTINATION TEST AND ENZYME-LINKED IMMUNOSORBENT ASSAY IN DETECTION OF Toxoplasma gondii ANTIBODY IN HUMAN SERA |
title |
EVALUATION OF MODIFIED AGGLUTINATION TEST AND ENZYME-LINKED IMMUNOSORBENT ASSAY IN DETECTION OF Toxoplasma gondii ANTIBODY IN HUMAN SERA |
spellingShingle |
EVALUATION OF MODIFIED AGGLUTINATION TEST AND ENZYME-LINKED IMMUNOSORBENT ASSAY IN DETECTION OF Toxoplasma gondii ANTIBODY IN HUMAN SERA Silva, Dayse Vaniele da |
title_short |
EVALUATION OF MODIFIED AGGLUTINATION TEST AND ENZYME-LINKED IMMUNOSORBENT ASSAY IN DETECTION OF Toxoplasma gondii ANTIBODY IN HUMAN SERA |
title_full |
EVALUATION OF MODIFIED AGGLUTINATION TEST AND ENZYME-LINKED IMMUNOSORBENT ASSAY IN DETECTION OF Toxoplasma gondii ANTIBODY IN HUMAN SERA |
title_fullStr |
EVALUATION OF MODIFIED AGGLUTINATION TEST AND ENZYME-LINKED IMMUNOSORBENT ASSAY IN DETECTION OF Toxoplasma gondii ANTIBODY IN HUMAN SERA |
title_full_unstemmed |
EVALUATION OF MODIFIED AGGLUTINATION TEST AND ENZYME-LINKED IMMUNOSORBENT ASSAY IN DETECTION OF Toxoplasma gondii ANTIBODY IN HUMAN SERA |
title_sort |
EVALUATION OF MODIFIED AGGLUTINATION TEST AND ENZYME-LINKED IMMUNOSORBENT ASSAY IN DETECTION OF Toxoplasma gondii ANTIBODY IN HUMAN SERA |
author |
Silva, Dayse Vaniele da |
author_facet |
Silva, Dayse Vaniele da Marques, Joil Moreira Correa, Nelton Anderson Bespalez Velasquez, Leonardo Garcia Silva, Rodrigo Costa da Langoni, Helio Söhsten, Adriana Lebram von Silva, Aristeu Vieira da |
author_role |
author |
author2 |
Marques, Joil Moreira Correa, Nelton Anderson Bespalez Velasquez, Leonardo Garcia Silva, Rodrigo Costa da Langoni, Helio Söhsten, Adriana Lebram von Silva, Aristeu Vieira da |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Dayse Vaniele da Marques, Joil Moreira Correa, Nelton Anderson Bespalez Velasquez, Leonardo Garcia Silva, Rodrigo Costa da Langoni, Helio Söhsten, Adriana Lebram von Silva, Aristeu Vieira da |
description |
Toxoplasmosis is a zoonosis of high prevalence around the world, leading to serious congenital alterations and in immune compromised individuals. Its diagnosis is based in the detection of serum antibodies. This work aimed to compare serum antibody to Toxoplasma gondii detection by ELISA for IgG antibodies, and the MAT with the results of IFAT. From 73 samples examined by IgG-IFAT, 53 (72.6%) were positive, IgG-ELISA 55 (75.4%) and MAT, 58 (79.4%). No significative differences among results of tests are found. MAT shows high sensibility (100%) and lower specificity (75%), there were not false-negative results, but showed false-positives (25%). ELISA showed sensibility of 98.1% and specificity of 85,0%, false-negative rate of 1.9% and false-positive rate of 15.0%. MAT performance, when compared with IFAT, suggests the utilization of this method in diagnostic and epidemiological preliminary tests, besides ELISA has been used as a confirmatory test, for its specificity. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-05-21 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.unipar.br/index.php/saude/article/view/4561 |
url |
https://revistas.unipar.br/index.php/saude/article/view/4561 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revistas.unipar.br/index.php/saude/article/view/4561/2749 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UNIPAR |
publisher.none.fl_str_mv |
UNIPAR |
dc.source.none.fl_str_mv |
Arquivos de Ciências da Saúde da UNIPAR; v. 16 n. 1 (2012) 1982-114X reponame:Arquivos de Ciências da Saúde da UNIPAR (Online) instname:Universidade Paranaense (UNIPAR) instacron:UNIPAR |
instname_str |
Universidade Paranaense (UNIPAR) |
instacron_str |
UNIPAR |
institution |
UNIPAR |
reponame_str |
Arquivos de Ciências da Saúde da UNIPAR (Online) |
collection |
Arquivos de Ciências da Saúde da UNIPAR (Online) |
repository.name.fl_str_mv |
Arquivos de Ciências da Saúde da UNIPAR (Online) - Universidade Paranaense (UNIPAR) |
repository.mail.fl_str_mv |
||cedic@unipar.br|| arqsaude@unipar.br |
_version_ |
1800218853732515840 |